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Protein

Proenkephalin-A

Gene

PENK

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endorphin, Neuropeptide, Opioid peptide

Names & Taxonomyi

Protein namesi
Recommended name:
Proenkephalin-A
Cleaved into the following 8 chains:
Gene namesi
Name:PENK
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PeptideiPRO_000000821925 – 97SynenkephalinAdd BLAST73
PeptideiPRO_0000008220100 – 104Met-enkephalin5
PeptideiPRO_0000008221107 – 111Met-enkephalin5
PeptideiPRO_0000377685114 – 133PENK(114-133)By similarityAdd BLAST20
PeptideiPRO_0000008223136 – 140Met-enkephalin5
PeptideiPRO_0000377686143 – 184PENK(143-184)By similarityAdd BLAST42
PeptideiPRO_0000008225187 – 194Met-enkephalin-Arg-Gly-Leu8
PropeptideiPRO_0000008226197 – 208Add BLAST12
PeptideiPRO_0000008227211 – 215Met-enkephalin5
PropeptideiPRO_0000008228218 – 228Add BLAST11
PeptideiPRO_0000008229231 – 235Leu-enkephalin5
PeptideiPRO_0000377687238 – 259PENK(238-259)By similarityAdd BLAST22
PeptideiPRO_0000008231262 – 268Met-enkephalin-Arg-Phe7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 48By similarity
Disulfide bondi30 ↔ 52By similarity
Disulfide bondi33 ↔ 65By similarity
Modified residuei252PhosphoserineBy similarity1

Post-translational modificationi

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000011497.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJUY. Eukaryota.
ENOG4110ZYD. LUCA.
HOGENOMiHOG000013003.
HOVERGENiHBG000063.
InParanoidiP47969.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLLRLCTW LVALGPGLLA TVQAECSQDC AACSYRLVRP GDLNFLACTL
60 70 80 90 100
ECEGQLPSLK IWETCKDLLQ VSKQELPQEG ASSLRESGKQ DESHLLSKKY
110 120 130 140 150
GGFMKRYGGF MKKVDELYPV EPEEEANGGE ILTKRYGGFM KKDAEDGDAL
160 170 180 190 200
ANSSDLLKEL LGTGDDRDRE NHHQEGGDSD EGVSKRYGGF MRGLKRSPQV
210 220 230 240 250
EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE FLPSDEEGES
260
YSKEVPEMEK RYGGFMRF
Length:268
Mass (Da):30,436
Last modified:February 1, 1996 - v1
Checksum:i64C85EE9F480F238
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16138 Genomic DNA. Translation: AAA87900.1.
PIRiA18864.
RefSeqiNP_001166888.1. NM_001173417.1.

Genome annotation databases

GeneIDi100379621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16138 Genomic DNA. Translation: AAA87900.1.
PIRiA18864.
RefSeqiNP_001166888.1. NM_001173417.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000011497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100379621.

Organism-specific databases

CTDi5179.

Phylogenomic databases

eggNOGiENOG410IJUY. Eukaryota.
ENOG4110ZYD. LUCA.
HOGENOMiHOG000013003.
HOVERGENiHBG000063.
InParanoidiP47969.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPENK_CAVPO
AccessioniPrimary (citable) accession number: P47969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.