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P47969 (PENK_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proenkephalin-A

Cleaved into the following 8 chains:

  1. Synenkephalin
  2. Met-enkephalin
    Alternative name(s):
    Opioid growth factor
    Short name=OGF
  3. PENK(114-133)
  4. PENK(143-184)
  5. Met-enkephalin-Arg-Gly-Leu
  6. Leu-enkephalin
  7. PENK(238-259)
  8. Met-enkephalin-Arg-Phe
Gene names
Name:PENK
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Ref.2

Subcellular location

Secreted.

Post-translational modification

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Sequence similarities

Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionEndorphin
Neuropeptide
Opioid peptide
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processneuropeptide signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Peptide25 – 9773Synenkephalin
PRO_0000008219
Peptide100 – 1045Met-enkephalin
PRO_0000008220
Peptide107 – 1115Met-enkephalin
PRO_0000008221
Peptide114 – 13320PENK(114-133) By similarity
PRO_0000377685
Peptide136 – 1405Met-enkephalin
PRO_0000008223
Peptide143 – 18442PENK(143-184) By similarity
PRO_0000377686
Peptide187 – 1948Met-enkephalin-Arg-Gly-Leu
PRO_0000008225
Propeptide197 – 20812
PRO_0000008226
Peptide211 – 2155Met-enkephalin
PRO_0000008227
Propeptide218 – 22811
PRO_0000008228
Peptide231 – 2355Leu-enkephalin
PRO_0000008229
Peptide238 – 25922PENK(238-259) By similarity
PRO_0000377687
Peptide262 – 2687Met-enkephalin-Arg-Phe
PRO_0000008231

Amino acid modifications

Disulfide bond26 ↔ 48 By similarity
Disulfide bond30 ↔ 52 By similarity
Disulfide bond33 ↔ 65 By similarity

Sequences

Sequence LengthMass (Da)Tools
P47969 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 64C85EE9F480F238

FASTA26830,436
        10         20         30         40         50         60 
MARLLRLCTW LVALGPGLLA TVQAECSQDC AACSYRLVRP GDLNFLACTL ECEGQLPSLK 

        70         80         90        100        110        120 
IWETCKDLLQ VSKQELPQEG ASSLRESGKQ DESHLLSKKY GGFMKRYGGF MKKVDELYPV 

       130        140        150        160        170        180 
EPEEEANGGE ILTKRYGGFM KKDAEDGDAL ANSSDLLKEL LGTGDDRDRE NHHQEGGDSD 

       190        200        210        220        230        240 
EGVSKRYGGF MRGLKRSPQV EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE 

       250        260 
FLPSDEEGES YSKEVPEMEK RYGGFMRF 

« Hide

References

[1]"Structure and expression of the guinea pig preproenkephalin gene: site-specific cleavage in the 3' untranslated region yields truncated mRNA transcripts in specific brain regions."
Laforge K.S., Unterwald E.M., Kreek M.J.
Mol. Cell. Biol. 15:2080-2089(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Hartley.
[2]"A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence."
Kilpatrick D.L., Taniguchi T., Jones B.N., Stern A.S., Shively J.E., Hullihan J., Kimura S., Stein S., Udenfriend S.
Proc. Natl. Acad. Sci. U.S.A. 78:3265-3268(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 211-235, POSSIBLE BIOLOGICAL FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16138 Genomic DNA. Translation: AAA87900.1.
PIRA18864.
RefSeqNP_001166888.1. NM_001173417.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000011497.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100379621.

Organism-specific databases

CTD110182.

Phylogenomic databases

eggNOGNOG40630.
HOGENOMHOG000013003.
HOVERGENHBG000063.
InParanoidP47969.

Family and domain databases

InterProIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePENK_CAVPO
AccessionPrimary (citable) accession number: P47969
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 13, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families