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P47969

- PENK_CAVPO

UniProt

P47969 - PENK_CAVPO

Protein

Proenkephalin-A

Gene

PENK

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

    GO - Biological processi

    1. neuropeptide signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endorphin, Neuropeptide, Opioid peptide

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proenkephalin-A
    Cleaved into the following 8 chains:
    Gene namesi
    Name:PENK
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Peptidei25 – 9773SynenkephalinPRO_0000008219Add
    BLAST
    Peptidei100 – 1045Met-enkephalinPRO_0000008220
    Peptidei107 – 1115Met-enkephalinPRO_0000008221
    Peptidei114 – 13320PENK(114-133)By similarityPRO_0000377685Add
    BLAST
    Peptidei136 – 1405Met-enkephalinPRO_0000008223
    Peptidei143 – 18442PENK(143-184)By similarityPRO_0000377686Add
    BLAST
    Peptidei187 – 1948Met-enkephalin-Arg-Gly-LeuPRO_0000008225
    Propeptidei197 – 20812PRO_0000008226Add
    BLAST
    Peptidei211 – 2155Met-enkephalinPRO_0000008227
    Propeptidei218 – 22811PRO_0000008228Add
    BLAST
    Peptidei231 – 2355Leu-enkephalinPRO_0000008229
    Peptidei238 – 25922PENK(238-259)By similarityPRO_0000377687Add
    BLAST
    Peptidei262 – 2687Met-enkephalin-Arg-PhePRO_0000008231

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 48By similarity
    Disulfide bondi30 ↔ 52By similarity
    Disulfide bondi33 ↔ 65By similarity

    Post-translational modificationi

    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000011497.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40630.
    HOGENOMiHOG000013003.
    HOVERGENiHBG000063.
    InParanoidiP47969.

    Family and domain databases

    InterProiIPR006024. Opioid_neupept.
    IPR000703. Proenkphlin_A.
    [Graphical view]
    PANTHERiPTHR11438. PTHR11438. 1 hit.
    PTHR11438:SF3. PTHR11438:SF3. 1 hit.
    PfamiPF01160. Opiods_neuropep. 1 hit.
    [Graphical view]
    PRINTSiPR01028. OPIOIDPRCRSR.
    PR01029. PENKAPRCRSR.
    PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P47969-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARLLRLCTW LVALGPGLLA TVQAECSQDC AACSYRLVRP GDLNFLACTL    50
    ECEGQLPSLK IWETCKDLLQ VSKQELPQEG ASSLRESGKQ DESHLLSKKY 100
    GGFMKRYGGF MKKVDELYPV EPEEEANGGE ILTKRYGGFM KKDAEDGDAL 150
    ANSSDLLKEL LGTGDDRDRE NHHQEGGDSD EGVSKRYGGF MRGLKRSPQV 200
    EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE FLPSDEEGES 250
    YSKEVPEMEK RYGGFMRF 268
    Length:268
    Mass (Da):30,436
    Last modified:February 1, 1996 - v1
    Checksum:i64C85EE9F480F238
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16138 Genomic DNA. Translation: AAA87900.1.
    PIRiA18864.
    RefSeqiNP_001166888.1. NM_001173417.1.

    Genome annotation databases

    GeneIDi100379621.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16138 Genomic DNA. Translation: AAA87900.1 .
    PIRi A18864.
    RefSeqi NP_001166888.1. NM_001173417.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000011497.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100379621.

    Organism-specific databases

    CTDi 110182.

    Phylogenomic databases

    eggNOGi NOG40630.
    HOGENOMi HOG000013003.
    HOVERGENi HBG000063.
    InParanoidi P47969.

    Family and domain databases

    InterProi IPR006024. Opioid_neupept.
    IPR000703. Proenkphlin_A.
    [Graphical view ]
    PANTHERi PTHR11438. PTHR11438. 1 hit.
    PTHR11438:SF3. PTHR11438:SF3. 1 hit.
    Pfami PF01160. Opiods_neuropep. 1 hit.
    [Graphical view ]
    PRINTSi PR01028. OPIOIDPRCRSR.
    PR01029. PENKAPRCRSR.
    PROSITEi PS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of the guinea pig preproenkephalin gene: site-specific cleavage in the 3' untranslated region yields truncated mRNA transcripts in specific brain regions."
      Laforge K.S., Unterwald E.M., Kreek M.J.
      Mol. Cell. Biol. 15:2080-2089(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Hartley.
    2. "A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence."
      Kilpatrick D.L., Taniguchi T., Jones B.N., Stern A.S., Shively J.E., Hullihan J., Kimura S., Stein S., Udenfriend S.
      Proc. Natl. Acad. Sci. U.S.A. 78:3265-3268(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 211-235, POSSIBLE BIOLOGICAL FUNCTION.

    Entry informationi

    Entry nameiPENK_CAVPO
    AccessioniPrimary (citable) accession number: P47969
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3