P47969 (PENK_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proenkephalin-A

Cleaved into the following 8 chains:

1. Synenkephalin
2. Met-enkephalin
   Alternative name(s):
   Opioid growth factor
   Short name=OGF
3. PENK(114-133)
4. PENK(143-184)
5. Met-enkephalin-Arg-Gly-Leu
6. Leu-enkephalin
7. PENK(238-259)
8. Met-enkephalin-Arg-Phe

Gene names

Name:

PENK

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Ref.2
Subcellular location	Secreted.
Post-translational modification	The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities	Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endorphin Neuropeptide Opioid peptide
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	behavioral fear response Inferred from electronic annotation. Source: Compara neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of pain Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Peptide	25 – 97	73	Synenkephalin		PRO_0000008219
	Peptide	100 – 104	5	Met-enkephalin		PRO_0000008220
	Peptide	107 – 111	5	Met-enkephalin		PRO_0000008221
	Peptide	114 – 133	20	PENK(114-133) By similarity		PRO_0000377685
	Peptide	136 – 140	5	Met-enkephalin		PRO_0000008223
	Peptide	143 – 184	42	PENK(143-184) By similarity		PRO_0000377686
	Peptide	187 – 194	8	Met-enkephalin-Arg-Gly-Leu		PRO_0000008225
	Propeptide	197 – 208	12			PRO_0000008226
	Peptide	211 – 215	5	Met-enkephalin		PRO_0000008227
	Propeptide	218 – 228	11			PRO_0000008228
	Peptide	231 – 235	5	Leu-enkephalin		PRO_0000008229
	Peptide	238 – 259	22	PENK(238-259) By similarity		PRO_0000377687
	Peptide	262 – 268	7	Met-enkephalin-Arg-Phe		PRO_0000008231
Amino acid modifications
	Disulfide bond	26 ↔ 48		By similarity
	Disulfide bond	30 ↔ 52		By similarity
	Disulfide bond	33 ↔ 65		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47969 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 64C85EE9F480F238	FASTA	268	30,436
	10 20 30 40 50 60 MARLLRLCTW LVALGPGLLA TVQAECSQDC AACSYRLVRP GDLNFLACTL ECEGQLPSLK 70 80 90 100 110 120 IWETCKDLLQ VSKQELPQEG ASSLRESGKQ DESHLLSKKY GGFMKRYGGF MKKVDELYPV 130 140 150 160 170 180 EPEEEANGGE ILTKRYGGFM KKDAEDGDAL ANSSDLLKEL LGTGDDRDRE NHHQEGGDSD 190 200 210 220 230 240 EGVSKRYGGF MRGLKRSPQV EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE 250 260 FLPSDEEGES YSKEVPEMEK RYGGFMRF

References

[1]	"Structure and expression of the guinea pig preproenkephalin gene: site-specific cleavage in the 3' untranslated region yields truncated mRNA transcripts in specific brain regions." Laforge K.S., Unterwald E.M., Kreek M.J. Mol. Cell. Biol. 15:2080-2089(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hartley.
[2]	"A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence." Kilpatrick D.L., Taniguchi T., Jones B.N., Stern A.S., Shively J.E., Hullihan J., Kimura S., Stein S., Udenfriend S. Proc. Natl. Acad. Sci. U.S.A. 78:3265-3268(1981) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 211-235, POSSIBLE BIOLOGICAL FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16138 Genomic DNA. Translation: AAA87900.1.
PIR	A18864.
RefSeq	NP_001166888.1. NM_001173417.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10141.ENSCPOP00000011497.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100379621.
Organism-specific databases
CTD	110182.
Phylogenomic databases
eggNOG	NOG40630.
HOGENOM	HOG000013003.
HOVERGEN	HBG000063.
InParanoid	P47969.
OrthoDB	EOG4HMJBF.
Family and domain databases
InterPro	IPR006024. Opioid_neupept. IPR000703. Proenkphlin_A. [Graphical view]
PANTHER	PTHR11438. PTHR11438. 1 hit. PTHR11438:SF3. PTHR11438:SF3. 1 hit.
Pfam	PF01160. Opiods_neuropep. 1 hit. [Graphical view]
PRINTS	PR01028. OPIOIDPRCRSR. PR01029. PENKAPRCRSR.
PROSITE	PS01252. OPIOIDS_PRECURSOR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PENK_CAVPO

Accession

Primary (citable) accession number: P47969

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program