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Protein

ATP-dependent RNA helicase eIF4A

Gene

tif1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 708ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-429947. Deadenylation of mRNA.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SPO-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase eIF4A (EC:3.6.4.13)
Alternative name(s):
Eukaryotic initiation factor 4A
Short name:
eIF-4A
Translation initiation factor 1
Gene namesi
Name:tif1
Synonyms:tif41
ORF Names:SPAC1006.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1006.07.
PomBaseiSPAC1006.07.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation initiation factor 4F complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392ATP-dependent RNA helicase eIF4APRO_0000054966Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47943.
PRIDEiP47943.

PTM databases

iPTMnetiP47943.

Interactioni

Subunit structurei

Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G (By similarity).By similarity

Protein-protein interaction databases

BioGridi279388. 12 interactions.
MINTiMINT-4690367.

Structurei

3D structure databases

ProteinModelPortaliP47943.
SMRiP47943. Positions 20-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 220171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini231 – 392162Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi19 – 4729Q motifAdd
BLAST
Motifi168 – 1714DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000268797.
InParanoidiP47943.
KOiK03257.
OMAiMRKLEMG.
OrthoDBiEOG7FNCJ0.
PhylomeDBiP47943.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDQLEDSVI ETNYDEVIDT FDDMNLKPEL LRGIYAYGFE RPSAIQQRAI
60 70 80 90 100
MPILGERDVL AQAQSGTGKT ATFSISVLQK IDTSLKQCQA LILAPTRELA
110 120 130 140 150
QQIQKVVVAL GDLMNVECHA CIGGTLVRDD MAALQAGVHV VVGTPGRVHD
160 170 180 190 200
MIQRRALPTD AVQMFVLDEA DEMLSRGFKD QIYDIFQLLP PTAQVVLLSA
210 220 230 240 250
TMPQDVLEVT TKFMRDPIRI LVKKDELTLE GIKQFYVAVE KEEWKLDTLC
260 270 280 290 300
DLYETVTVTQ AVIFCNTRRK VDWLTEQLTE RDFTVSSMHG DMDQAQRDTL
310 320 330 340 350
MHEFRTGSSR ILITTDLLAR GIDVQQVSLV INYDLPANRE NYIHRIGRGG
360 370 380 390
RFGRKGVSIN FVTNDDVRMM REIEQFYNTH IEEMPMNIAD LI
Length:392
Mass (Da):44,436
Last modified:October 1, 1996 - v2
Checksum:iABEB12265B4AE34E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80796 mRNA. Translation: CAA56772.1.
L40627 mRNA. Translation: AAB61679.1.
CU329670 Genomic DNA. Translation: CAB60237.1.
PIRiS71745.
RefSeqiNP_594854.1. NM_001020283.2.

Genome annotation databases

EnsemblFungiiSPAC1006.07.1; SPAC1006.07.1:pep; SPAC1006.07.
GeneIDi2542948.
KEGGispo:SPAC1006.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80796 mRNA. Translation: CAA56772.1.
L40627 mRNA. Translation: AAB61679.1.
CU329670 Genomic DNA. Translation: CAB60237.1.
PIRiS71745.
RefSeqiNP_594854.1. NM_001020283.2.

3D structure databases

ProteinModelPortaliP47943.
SMRiP47943. Positions 20-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279388. 12 interactions.
MINTiMINT-4690367.

PTM databases

iPTMnetiP47943.

Proteomic databases

MaxQBiP47943.
PRIDEiP47943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1006.07.1; SPAC1006.07.1:pep; SPAC1006.07.
GeneIDi2542948.
KEGGispo:SPAC1006.07.

Organism-specific databases

EuPathDBiFungiDB:SPAC1006.07.
PomBaseiSPAC1006.07.

Phylogenomic databases

HOGENOMiHOG000268797.
InParanoidiP47943.
KOiK03257.
OMAiMRKLEMG.
OrthoDBiEOG7FNCJ0.
PhylomeDBiP47943.

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-429947. Deadenylation of mRNA.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SPO-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

PROiP47943.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The translation initiation factor eIF4A from Schizosaccharomyces pombe is closely related to its mammalian counterpart."
    Fischli A., Schmid S.R., Coppolecchia R., Linder P.
    Yeast 12:977-981(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Daga R.R., Jimenez J.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiIF4A_SCHPO
AccessioniPrimary (citable) accession number: P47943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.