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Protein

ATP-dependent RNA helicase eIF4A

Gene

tif1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 70ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: PomBase
  • translation initiation factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational initiation Source: PomBase
  • regulation of gene expression Source: GO_Central
  • RNA secondary structure unwinding Source: GO_Central
  • translational initiation Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SPO-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-SPO-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase eIF4A (EC:3.6.4.13)
Alternative name(s):
Eukaryotic initiation factor 4A
Short name:
eIF-4A
Translation initiation factor 1
Gene namesi
Name:tif1
Synonyms:tif41
ORF Names:SPAC1006.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1006.07
PomBaseiSPAC1006.07

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549661 – 392ATP-dependent RNA helicase eIF4AAdd BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47943
PaxDbiP47943
PRIDEiP47943

PTM databases

iPTMnetiP47943

Interactioni

Subunit structurei

Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G (By similarity).By similarity

Protein-protein interaction databases

BioGridi279388, 11 interactors
STRINGi4896.SPAC1006.07.1

Structurei

3D structure databases

ProteinModelPortaliP47943
SMRiP47943
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 220Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini231 – 392Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi19 – 47Q motifAdd BLAST29
Motifi168 – 171DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000268797
InParanoidiP47943
KOiK03257
OMAiRNPIRIL
OrthoDBiEOG092C2B1L
PhylomeDBiP47943

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P47943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDQLEDSVI ETNYDEVIDT FDDMNLKPEL LRGIYAYGFE RPSAIQQRAI
60 70 80 90 100
MPILGERDVL AQAQSGTGKT ATFSISVLQK IDTSLKQCQA LILAPTRELA
110 120 130 140 150
QQIQKVVVAL GDLMNVECHA CIGGTLVRDD MAALQAGVHV VVGTPGRVHD
160 170 180 190 200
MIQRRALPTD AVQMFVLDEA DEMLSRGFKD QIYDIFQLLP PTAQVVLLSA
210 220 230 240 250
TMPQDVLEVT TKFMRDPIRI LVKKDELTLE GIKQFYVAVE KEEWKLDTLC
260 270 280 290 300
DLYETVTVTQ AVIFCNTRRK VDWLTEQLTE RDFTVSSMHG DMDQAQRDTL
310 320 330 340 350
MHEFRTGSSR ILITTDLLAR GIDVQQVSLV INYDLPANRE NYIHRIGRGG
360 370 380 390
RFGRKGVSIN FVTNDDVRMM REIEQFYNTH IEEMPMNIAD LI
Length:392
Mass (Da):44,436
Last modified:October 1, 1996 - v2
Checksum:iABEB12265B4AE34E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80796 mRNA Translation: CAA56772.1
L40627 mRNA Translation: AAB61679.1
CU329670 Genomic DNA Translation: CAB60237.1
PIRiS71745
RefSeqiNP_594854.1, NM_001020283.2

Genome annotation databases

EnsemblFungiiSPAC1006.07.1; SPAC1006.07.1:pep; SPAC1006.07
GeneIDi2542948
KEGGispo:SPAC1006.07

Similar proteinsi

Entry informationi

Entry nameiIF4A_SCHPO
AccessioniPrimary (citable) accession number: P47943
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

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