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P47942

- DPYL2_RAT

UniProt

P47942 - DPYL2_RAT

Protein

Dihydropyrimidinase-related protein 2

Gene

Dpysl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei486 – 4861Not phosphorylated

    GO - Molecular functioni

    1. hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. brain development Source: RGD
    2. cytoskeleton organization Source: UniProtKB
    3. endocytosis Source: UniProtKB
    4. olfactory bulb development Source: RGD
    5. positive regulation of glutamate secretion Source: RGD
    6. pyrimidine nucleobase catabolic process Source: InterPro
    7. regulation of axon extension Source: RGD
    8. regulation of neuron differentiation Source: RGD
    9. response to amphetamine Source: RGD
    10. response to cocaine Source: RGD
    11. response to drug Source: RGD
    12. spinal cord development Source: RGD
    13. synaptic vesicle transport Source: RGD

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis

    Protein family/group databases

    MEROPSiM38.975.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydropyrimidinase-related protein 2
    Short name:
    DRP-2
    Alternative name(s):
    Collapsin response mediator protein 2
    Short name:
    CRMP-2
    Turned on after division 64 kDa protein
    Short name:
    TOAD-64
    Gene namesi
    Name:Dpysl2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 15

    Organism-specific databases

    RGDi2517. Dpysl2.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cytoplasmcytoskeleton By similarity. Membrane By similarity
    Note: Tightly but noncovalently associated with membranes.1 Publication

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. cytosol Source: RGD
    3. dendrite Source: RGD
    4. growth cone Source: RGD
    5. membrane Source: UniProtKB-SubCell
    6. microtubule Source: Ensembl
    7. mitochondrion Source: Ensembl
    8. neuronal cell body Source: RGD
    9. neuron projection Source: RGD
    10. protein complex Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 572572Dihydropyrimidinase-related protein 2PRO_0000165915Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei32 – 321Phosphotyrosine; by FYNBy similarity
    Modified residuei258 – 2581N6-succinyllysineBy similarity
    Modified residuei431 – 4311PhosphotyrosineBy similarity
    Modified residuei465 – 4651PhosphoserineBy similarity
    Modified residuei499 – 4991PhosphotyrosineBy similarity
    Modified residuei504 – 5041S-nitrosocysteine1 Publication
    Modified residuei509 – 5091Phosphothreonine2 Publications
    Modified residuei514 – 5141Phosphothreonine; by GSK3-betaBy similarity
    Modified residuei517 – 5171PhosphoserineBy similarity
    Modified residuei518 – 5181Phosphoserine1 Publication
    Modified residuei522 – 5221Phosphoserine; alternate1 Publication
    Modified residuei522 – 5221Phosphoserine; by DYRK2; alternateBy similarity
    Modified residuei555 – 5551Phosphothreonine; by ROCK2By similarity

    Post-translational modificationi

    Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B. Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, S-nitrosylation

    Proteomic databases

    PaxDbiP47942.
    PRIDEiP47942.

    2D gel databases

    World-2DPAGE0004:P47942.

    PTM databases

    PhosphoSiteiP47942.

    Expressioni

    Developmental stagei

    Expressed immediately after neuronal birth and is dramatically down-regulated in the adult.

    Gene expression databases

    GenevestigatoriP47942.

    Interactioni

    Subunit structurei

    Homotetramer, and heterotetramer with CRMP1, DPYSL3, DPYSL4 or DPYSL5. Interacts through its C-terminus with the C-terminus of CYFIP1/SRA1. Interacts with HTR4. Interacts with CLN6. Interacts with MICALL1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ppp2caP633312EBI-917570,EBI-7050205

    Protein-protein interaction databases

    BioGridi247451. 6 interactions.
    IntActiP47942. 4 interactions.
    MINTiMINT-224453.
    STRINGi10116.ENSRNOP00000012996.

    Structurei

    3D structure databases

    ProteinModelPortaliP47942.
    SMRiP47942. Positions 14-490.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0044.
    GeneTreeiENSGT00740000115123.
    HOGENOMiHOG000219145.
    HOVERGENiHBG000806.
    InParanoidiP47942.
    KOiK07528.
    OMAiEVPAFFK.
    OrthoDBiEOG7SJD48.
    PhylomeDBiP47942.
    TreeFamiTF314706.

    Family and domain databases

    Gene3Di2.30.40.10. 2 hits.
    InterProiIPR006680. Amidohydro_1.
    IPR011778. Hydantoinase/dihydroPyrase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view]
    PfamiPF01979. Amidohydro_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 2 hits.
    TIGRFAMsiTIGR02033. D-hydantoinase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P47942-1 [UniParc]FASTAAdd to Basket

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    MSYQGKKNIP RITSDRLLIK GGKIVNDDQS FYADIYMEDG LIKQIGENLI    50
    VPGGVKTIEA HSRMVIPGGI DVHTRFQMPD QGMTSADDFF QGTKAALAGG 100
    TTMIIDHVVP EPGTSLLAAF DQWREWADSK SCCDYSLHVD ITEWHKGIQE 150
    EMEALVKDHG VNSFLVYMAF KDRFQLTDSQ IYEVLSVIRD IGAIAQVHAE 200
    NGDIIAEEQQ RILDLGITGP EGHVLSRPEE VEAEAVNRSI TIANQTNCPL 250
    YVTKVMSKSA AEVIAQARKK GTVVYGEPIT ASLGTDGSHY WSKNWAKAAA 300
    FVTSPPLSPD PTTPDFLNSL LSCGDLQVTG SAHCTFNTAQ KAVGKDNFTL 350
    IPEGTNGTEE RMSVIWDKAV VTGKMDENQF VAVTSTNAAK VFNLYPRKGR 400
    ISVGSDADLV IWDPDSVKTI SAKTHNSALE YNIFEGMECR GSPLVVISQG 450
    KIVLEDGTLH VTEGSGRYIP RKPFPDFVYK RIKARSRLAE LRGVPRGLYD 500
    GPVCEVSVTP KTVTPASSAK TSPAKQQAPP VRNLHQSGFS LSGAQIDDNI 550
    PRRTTQRIVA PPGGRANITS LG 572
    Length:572
    Mass (Da):62,278
    Last modified:February 1, 1996 - v1
    Checksum:iC031F3BC038AA737
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46882 mRNA. Translation: CAA86981.1.
    PIRiA59280. S49985.
    RefSeqiNP_001099187.1. NM_001105717.2.
    UniGeneiRn.2889.

    Genome annotation databases

    EnsembliENSRNOT00000012996; ENSRNOP00000012996; ENSRNOG00000009625.
    GeneIDi25416.
    KEGGirno:25416.
    UCSCiRGD:2517. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46882 mRNA. Translation: CAA86981.1 .
    PIRi A59280. S49985.
    RefSeqi NP_001099187.1. NM_001105717.2.
    UniGenei Rn.2889.

    3D structure databases

    ProteinModelPortali P47942.
    SMRi P47942. Positions 14-490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247451. 6 interactions.
    IntActi P47942. 4 interactions.
    MINTi MINT-224453.
    STRINGi 10116.ENSRNOP00000012996.

    Protein family/group databases

    MEROPSi M38.975.

    PTM databases

    PhosphoSitei P47942.

    2D gel databases

    World-2DPAGE 0004:P47942.

    Proteomic databases

    PaxDbi P47942.
    PRIDEi P47942.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012996 ; ENSRNOP00000012996 ; ENSRNOG00000009625 .
    GeneIDi 25416.
    KEGGi rno:25416.
    UCSCi RGD:2517. rat.

    Organism-specific databases

    CTDi 1808.
    RGDi 2517. Dpysl2.

    Phylogenomic databases

    eggNOGi COG0044.
    GeneTreei ENSGT00740000115123.
    HOGENOMi HOG000219145.
    HOVERGENi HBG000806.
    InParanoidi P47942.
    KOi K07528.
    OMAi EVPAFFK.
    OrthoDBi EOG7SJD48.
    PhylomeDBi P47942.
    TreeFami TF314706.

    Miscellaneous databases

    NextBioi 606559.
    PROi P47942.

    Gene expression databases

    Genevestigatori P47942.

    Family and domain databases

    Gene3Di 2.30.40.10. 2 hits.
    InterProi IPR006680. Amidohydro_1.
    IPR011778. Hydantoinase/dihydroPyrase.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view ]
    Pfami PF01979. Amidohydro_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51338. SSF51338. 2 hits.
    TIGRFAMsi TIGR02033. D-hydantoinase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "TOAD-64, a gene expressed early in neuronal differentiation in the rat, is related to unc-33, a C. elegans gene involved in axon outgrowth."
      Minturn J.E., Fryer H.J.L., Geschwind D.H., Hockfield S.
      J. Neurosci. 15:6757-6766(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 136-142; 402-418; 441-450 AND 499-511, SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 44-56; 76-94; 147-157; 174-238; 259-268; 375-397; 424-467; 472-480; 533-552 AND 558-565, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.
    3. "Brain CRMP forms heterotetramers similar to liver dihydropyrimidinase."
      Wang L.H., Strittmatter S.M.
      J. Neurochem. 69:2261-2269(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CRMP1; DPYSL3 AND DPYSL4, SUBUNIT.
    4. "Neurofibrillary tangle-associated collapsin response mediator protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518, and Ser-522."
      Gu Y., Hamajima N., Ihara Y.
      Biochemistry 39:4267-4275(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-509; SER-518 AND SER-522, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "SNOSID, a proteomic method for identification of cysteine S-nitrosylation sites in complex protein mixtures."
      Hao G., Derakhshan B., Shi L., Campagne F., Gross S.S.
      Proc. Natl. Acad. Sci. U.S.A. 103:1012-1017(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: S-NITROSYLATION [LARGE SCALE ANALYSIS] AT CYS-504, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Lubec G., Chen W.-Q.
      Submitted (FEB-2007) to UniProtKB
      Cited for: PHOSPHORYLATION AT THR-509, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiDPYL2_RAT
    AccessioniPrimary (citable) accession number: P47942
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Lacks most of the conserved residues that are essential for binding the metal cofactor and hence for dihydropyrimidinase activity. Its enzyme activity is therefore unsure.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3