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Protein

Cannabinoid receptor 2

Gene

Cnr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.8 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Inflammatory response

Enzyme and pathway databases

ReactomeiR-MMU-373076. Class A/1 (Rhodopsin-like receptors).
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Cannabinoid receptor 2
Short name:
CB-2
Short name:
CB2
Short name:
mCB2
Gene namesi
Name:Cnr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:104650. Cnr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333ExtracellularSequence analysisAdd
BLAST
Transmembranei34 – 5926Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini60 – 7112CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9221Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini93 – 10412ExtracellularSequence analysisAdd
BLAST
Transmembranei105 – 12925Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini130 – 14920CytoplasmicSequence analysisAdd
BLAST
Transmembranei150 – 17223Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini173 – 18816ExtracellularSequence analysisAdd
BLAST
Transmembranei189 – 21426Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini215 – 24632CytoplasmicSequence analysisAdd
BLAST
Transmembranei247 – 26721Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini268 – 27912ExtracellularSequence analysisAdd
BLAST
Transmembranei280 – 30122Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini302 – 34746CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice are responsive to the psychotropic effects of cannabinoid but not to the cannabinoid-induced immunomodulation. They also show accelerated age-related trabecular bone loss and cortical expansion.1 Publication

Chemistry

ChEMBLiCHEMBL2111415.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Cannabinoid receptor 2PRO_0000069324Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi11 – 111N-linked (GlcNAc...)Sequence analysis
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei336 – 3361PhosphoserineCombined sources
Modified residuei338 – 3381PhosphothreonineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP47936.
PaxDbiP47936.
PRIDEiP47936.

PTM databases

iPTMnetiP47936.
PhosphoSiteiP47936.

Expressioni

Tissue specificityi

Expressed by cells of hematopoietic origin. Expressed in skin in suprabasal layers and hair follicles, in brain by neurons and glial cells and by osteoblasts, osteocytes, osteoclasts (at protein level).5 Publications

Gene expression databases

BgeeiP47936.
CleanExiMM_CNR2.
GenevisibleiP47936. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069957.

Chemistry

BindingDBiP47936.

Structurei

3D structure databases

ProteinModelPortaliP47936.
SMRiP47936. Positions 27-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiP47936.
KOiK04278.
OMAiLAHWKKC.
OrthoDBiEOG7CK36K.
PhylomeDBiP47936.
TreeFamiTF330052.

Family and domain databases

InterProiIPR001551. Canbinoid_rcpt_2.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR22750:SF10. PTHR22750:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00523. CANABINOID2R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47936-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGCRETEVT NGSNGGLEFN PMKEYMILSS GQQIAVAVLC TLMGLLSALE
60 70 80 90 100
NMAVLYIILS SRRLRRKPSY LFISSLAGAD FLASVIFACN FVIFHVFHGV
110 120 130 140 150
DSNAIFLLKI GSVTMTFTAS VGSLLLTAVD RYLCLCYPPT YKALVTRGRA
160 170 180 190 200
LVALCVMWVL SALISYLPLM GWTCCPSPCS ELFPLIPNDY LLGWLLFIAI
210 220 230 240 250
LFSGIIYTYG YVLWKAHRHV ATLAEHQDRQ VPGIARMRLD VRLAKTLGLV
260 270 280 290 300
LAVLLICWFP ALALMGHSLV TTLSDQVKEA FAFCSMLCLV NSMVNPIIYA
310 320 330 340
LRSGEIRSAA QHCLIGWKKY LQGLGPEGKE EGPRSSVTET EADVKTT
Length:347
Mass (Da):38,213
Last modified:February 1, 1996 - v1
Checksum:i10747611D41FF442
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641L → V in CAA63655 (PubMed:9261404).Curated
Sequence conflicti126 – 1261L → V in CAA63655 (PubMed:9261404).Curated
Sequence conflicti272 – 2721T → M in AAA63757 (Ref. 3) Curated
Sequence conflicti332 – 3321G → A in AAA63757 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86405 mRNA. Translation: CAA60159.1.
X93168 mRNA. Translation: CAA63655.1.
U21681 Genomic DNA. Translation: AAA63757.1.
AK036658 mRNA. Translation: BAC29520.1.
AK037898 mRNA. Translation: BAC29894.1.
AK134109 mRNA. Translation: BAE22017.1.
AL672076 Genomic DNA. Translation: CAM15899.1.
CH466552 Genomic DNA. Translation: EDL29965.1.
BC024052 mRNA. Translation: AAH24052.1.
CCDSiCCDS18793.1.
PIRiS70364.
RefSeqiNP_001292207.1. NM_001305278.1.
NP_034054.3. NM_009924.4.
UniGeneiMm.297251.

Genome annotation databases

EnsembliENSMUST00000068830; ENSMUSP00000069957; ENSMUSG00000062585.
ENSMUST00000097843; ENSMUSP00000095454; ENSMUSG00000062585.
GeneIDi12802.
KEGGimmu:12802.
UCSCiuc008vhh.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86405 mRNA. Translation: CAA60159.1.
X93168 mRNA. Translation: CAA63655.1.
U21681 Genomic DNA. Translation: AAA63757.1.
AK036658 mRNA. Translation: BAC29520.1.
AK037898 mRNA. Translation: BAC29894.1.
AK134109 mRNA. Translation: BAE22017.1.
AL672076 Genomic DNA. Translation: CAM15899.1.
CH466552 Genomic DNA. Translation: EDL29965.1.
BC024052 mRNA. Translation: AAH24052.1.
CCDSiCCDS18793.1.
PIRiS70364.
RefSeqiNP_001292207.1. NM_001305278.1.
NP_034054.3. NM_009924.4.
UniGeneiMm.297251.

3D structure databases

ProteinModelPortaliP47936.
SMRiP47936. Positions 27-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069957.

Chemistry

BindingDBiP47936.
ChEMBLiCHEMBL2111415.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP47936.
PhosphoSiteiP47936.

Proteomic databases

MaxQBiP47936.
PaxDbiP47936.
PRIDEiP47936.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068830; ENSMUSP00000069957; ENSMUSG00000062585.
ENSMUST00000097843; ENSMUSP00000095454; ENSMUSG00000062585.
GeneIDi12802.
KEGGimmu:12802.
UCSCiuc008vhh.3. mouse.

Organism-specific databases

CTDi1269.
MGIiMGI:104650. Cnr2.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiP47936.
KOiK04278.
OMAiLAHWKKC.
OrthoDBiEOG7CK36K.
PhylomeDBiP47936.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiR-MMU-373076. Class A/1 (Rhodopsin-like receptors).
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP47936.
SOURCEiSearch...

Gene expression databases

BgeeiP47936.
CleanExiMM_CNR2.
GenevisibleiP47936. MM.

Family and domain databases

InterProiIPR001551. Canbinoid_rcpt_2.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR22750:SF10. PTHR22750:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00523. CANABINOID2R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, expression and function of the murine CB2 peripheral cannabinoid receptor."
    Shire D., Calandra B., Rinaldi-Carmona M., Oustric D., Pessegue B., Cabanne O., le Fur G., Caput D., Ferrara P.
    Biochim. Biophys. Acta 1307:132-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: C57BL/6J.
    Tissue: Spleen.
  2. "The genes encoding the peripheral cannabinoid receptor and alpha-L-fucosidase are located near a newly identified common virus integration site, Evi11."
    Valk P.J.M., Hol S., Vankan Y., Ihle J.N., Askew D., Jenkins N.A., Gilbert D.J., Copeland N.G., de Both N.J., Loewenberg B., Delwel R.
    J. Virol. 71:6796-6804(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NFS/N.
  3. Buckley N.E., Bonner T.I.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone and Thymus.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  8. "Immunomodulation by cannabinoids is absent in mice deficient for the cannabinoid CB(2) receptor."
    Buckley N.E., McCoy K.L., Mezey E., Bonner T., Zimmer A., Felder C.C., Glass M., Zimmer A.
    Eur. J. Pharmacol. 396:141-149(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  9. "Hematopoietic cells expressing the peripheral cannabinoid receptor migrate in response to the endocannabinoid 2-arachidonoylglycerol."
    Jorda M.A., Verbakel S.E., Valk P.J.M., Vankan-Berkhoudt Y.V., Maccarrone M., Finazzi-Agro A., Loewenberg B., Delwel R.
    Blood 99:2786-2793(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  10. "Inhibition of skin tumor growth and angiogenesis in vivo by activation of cannabinoid receptors."
    Casanova M.L., Blazquez C., Martinez-Palacio J., Villanueva C., Fernandez-Acenero M.J., Huffman J.W., Jorcano J.L., Guzman M.
    J. Clin. Invest. 111:43-50(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  11. "Formation of B and T cell subsets require the cannabinoid receptor CB2."
    Ziring D., Wei B., Velazquez P., Schrage M., Buckley N.E., Braun J.
    Immunogenetics 58:714-725(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  12. Cited for: FUNCTION.
  13. Cited for: FUNCTION, TISSUE SPECIFICITY.
  14. "Direct suppression of CNS autoimmune inflammation via the cannabinoid receptor CB1 on neurons and CB2 on autoreactive T cells."
    Maresz K., Pryce G., Ponomarev E.D., Marsicano G., Croxford J.L., Shriver L.P., Ledent C., Cheng X., Carrier E.J., Mann M.K., Giovannoni G., Pertwee R.G., Yamamura T., Buckley N.E., Hillard C.J., Lutz B., Baker D., Dittel B.N.
    Nat. Med. 13:492-497(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. Cited for: FUNCTION, TISSUE SPECIFICITY.
  16. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335; SER-336 AND THR-338, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-338, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiCNR2_MOUSE
AccessioniPrimary (citable) accession number: P47936
Secondary accession number(s): Q544H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.