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Protein

DNA-binding protein inhibitor ID-4

Gene

ID4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation (By similarity).By similarity

GO - Molecular functioni

  1. transcription corepressor activity Source: ProtInc

GO - Biological processi

  1. cellular protein localization Source: Ensembl
  2. central nervous system myelination Source: Ensembl
  3. cerebral cortex neuron differentiation Source: Ensembl
  4. circadian rhythm Source: Ensembl
  5. fat cell differentiation Source: Ensembl
  6. G1/S transition of mitotic cell cycle Source: Ensembl
  7. hippocampus development Source: Ensembl
  8. negative regulation of astrocyte differentiation Source: Ensembl
  9. negative regulation of fat cell differentiation Source: Ensembl
  10. negative regulation of neuron differentiation Source: Ensembl
  11. negative regulation of oligodendrocyte differentiation Source: Ensembl
  12. negative regulation of transcription, DNA-templated Source: GDB
  13. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  14. neuroblast proliferation Source: Ensembl
  15. positive regulation of cell proliferation Source: Ensembl
  16. positive regulation of osteoblast differentiation Source: Ensembl
  17. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  18. regulation of transcription from RNA polymerase II promoter Source: ProtInc
  19. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-4
Alternative name(s):
Class B basic helix-loop-helix protein 27
Short name:
bHLHb27
Inhibitor of DNA binding 4
Inhibitor of differentiation 4
Gene namesi
Name:ID4
Synonyms:BHLHB27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:5363. ID4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29611.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161DNA-binding protein inhibitor ID-4PRO_0000127250Add
BLAST

Proteomic databases

MaxQBiP47928.
PaxDbiP47928.
PRIDEiP47928.

Expressioni

Gene expression databases

BgeeiP47928.
CleanExiHS_ID4.
GenevestigatoriP47928.

Organism-specific databases

HPAiCAB022407.
HPA045710.

Interactioni

Subunit structurei

Heterodimer with other HLH proteins.

Binary interactionsi

WithEntry#Exp.IntActNotes
NCK1P163333EBI-1754719,EBI-389883

Protein-protein interaction databases

BioGridi109626. 1 interaction.
IntActiP47928. 6 interactions.
MINTiMINT-7241891.
STRINGi9606.ENSP00000367972.

Structurei

3D structure databases

ProteinModelPortaliP47928.
SMRiP47928. Positions 67-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 10453bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi39 – 4810Poly-Ala
Compositional biasi118 – 1247Poly-Pro

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG74134.
GeneTreeiENSGT00390000000908.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP47928.
KOiK17695.
OMAiGACPVAP.
OrthoDBiEOG7X9G8G.
PhylomeDBiP47928.
TreeFamiTF326217.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC
60 70 80 90 100
KAAEAAADEP ALCLQCDMND CYSRLRRLVP TIPPNKKVSK VEILQHVIDY
110 120 130 140 150
ILDLQLALET HPALLRQPPP PAPPHHPAGT CPAAPPRTPL TALNTDPAGA
160
VNKQGDSILC R
Length:161
Mass (Da):16,622
Last modified:January 31, 1996 - v1
Checksum:i5B14847AE7337339
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 145SGRKA → RPLR in AAA82882 (PubMed:7665172).Curated
Sequence conflicti39 – 402AA → Q in AAA82882 (PubMed:7665172).Curated
Sequence conflicti77 – 793RLV → WL in AAA82882 (PubMed:7665172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28368 mRNA. Translation: AAA73923.1.
Y07958 mRNA. Translation: CAA69255.1.
U16153 mRNA. Translation: AAA82882.1.
AL022726 Genomic DNA. Translation: CAA18779.1.
BC014941 mRNA. Translation: AAH14941.1.
CCDSiCCDS4544.1.
PIRiG01855.
JE0306.
RefSeqiNP_001537.1. NM_001546.3.
UniGeneiHs.519601.
Hs.663469.

Genome annotation databases

EnsembliENST00000378700; ENSP00000367972; ENSG00000172201.
GeneIDi3400.
KEGGihsa:3400.
UCSCiuc003ncw.4. human.

Polymorphism databases

DMDMi1352422.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28368 mRNA. Translation: AAA73923.1.
Y07958 mRNA. Translation: CAA69255.1.
U16153 mRNA. Translation: AAA82882.1.
AL022726 Genomic DNA. Translation: CAA18779.1.
BC014941 mRNA. Translation: AAH14941.1.
CCDSiCCDS4544.1.
PIRiG01855.
JE0306.
RefSeqiNP_001537.1. NM_001546.3.
UniGeneiHs.519601.
Hs.663469.

3D structure databases

ProteinModelPortaliP47928.
SMRiP47928. Positions 67-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109626. 1 interaction.
IntActiP47928. 6 interactions.
MINTiMINT-7241891.
STRINGi9606.ENSP00000367972.

Polymorphism databases

DMDMi1352422.

Proteomic databases

MaxQBiP47928.
PaxDbiP47928.
PRIDEiP47928.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378700; ENSP00000367972; ENSG00000172201.
GeneIDi3400.
KEGGihsa:3400.
UCSCiuc003ncw.4. human.

Organism-specific databases

CTDi3400.
GeneCardsiGC06P019837.
HGNCiHGNC:5363. ID4.
HPAiCAB022407.
HPA045710.
MIMi600581. gene.
neXtProtiNX_P47928.
PharmGKBiPA29611.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG74134.
GeneTreeiENSGT00390000000908.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP47928.
KOiK17695.
OMAiGACPVAP.
OrthoDBiEOG7X9G8G.
PhylomeDBiP47928.
TreeFamiTF326217.

Miscellaneous databases

GeneWikiiID4.
GenomeRNAii3400.
NextBioi13436.
PROiP47928.
SOURCEiSearch...

Gene expression databases

BgeeiP47928.
CleanExiHS_ID4.
GenevestigatoriP47928.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Kiesling T.L., Christy B.A.
    Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Abdominal adipose tissue.
  2. "cDNA cloning, tissue distribution and chromosomal localization of the human ID4 gene."
    Rigolet M., Rich T., Gross-Morand M.S., Molina-Gomes D., Viegas-Pequignot E., Junien C.
    DNA Res. 5:309-313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Molecular cloning of ID4, a novel dominant negative helix-loop-helix human gene on chromosome 6p21.3-p22."
    Pagliuca A., Bartoli P.C., Saccone S., Della Valle G., Lania L.
    Genomics 27:200-203(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.

Entry informationi

Entry nameiID4_HUMAN
AccessioniPrimary (citable) accession number: P47928
Secondary accession number(s): Q13005
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: January 31, 1996
Last modified: March 3, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.