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P47927

- AP2_ARATH

UniProt

P47927 - AP2_ARATH

Protein

Floral homeotic protein APETALA 2

Gene

AP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Probable transcriptional activator that promotes early floral meristem identity. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Plays a central role in the specification of floral identity, particularly for the normal development of sepals and petals in the wild-type flower. Acts as A class cadastral protein by repressing the C class floral homeotic gene AGAMOUS in association with other repressors like LEUNIG and SEUSS. It is also required during seed development.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi131 – 18757AP2/ERF 1PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi223 – 28058AP2/ERF 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. flower development Source: TAIR
    3. meristem maintenance Source: TAIR
    4. ovule development Source: TAIR
    5. seed development Source: TAIR
    6. specification of floral organ identity Source: TAIR
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Differentiation, Flowering, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Floral homeotic protein APETALA 2
    Gene namesi
    Name:AP2
    Ordered Locus Names:At4g36920
    ORF Names:AP22.49, C7A10.440
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G36920.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi159 – 1591G → E in AP2-5; temperature-sensitive.
    Mutagenesisi251 – 2511G → S in AP2-1; temperature-sensitive.
    Mutagenesisi420 – 4201Q → E in AP2-5; temperature-sensitive.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 432432Floral homeotic protein APETALA 2PRO_0000112522Add
    BLAST

    Proteomic databases

    PRIDEiP47927.

    Expressioni

    Tissue specificityi

    Sepals, petals, stamens, carpels, developing ovules, inflorescence stem, leaf and stem.

    Developmental stagei

    It is detectable at low levels throughout the shoot apex and at enhanced levels in the inflorescence meristem, young floral buds and throughout the early stages of flower development and organogenesis. During floral organ differentiation it becomes spatially restricted to specific organ, tissue and cell types within the flower.

    Inductioni

    Negatively regulated by the C class floral homeotic protein AGAMOUS in stamens and carpels. MicroRNA 172 (miRNA172) negatively regulates APETALA2 at the translational level and may modulate its expression pattern. Seems not to be influenced by jasmonate and Alternaria brassicicola.3 Publications

    Gene expression databases

    GenevestigatoriP47927.

    Interactioni

    Subunit structurei

    May form homodimer.

    Protein-protein interaction databases

    BioGridi15126. 4 interactions.
    STRINGi3702.AT4G36920.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP47927.
    SMRiP47927. Positions 131-189, 223-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi119 – 12810Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi14 – 5037Asp/Glu/Ser-rich (acidic; potential involvement with transcription)Add
    BLAST

    Sequence similaritiesi

    Contains 2 AP2/ERF DNA-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG247476.
    HOGENOMiHOG000033977.
    InParanoidiP47927.
    KOiK09284.
    OMAiESEEFCY.
    PhylomeDBiP47927.

    Family and domain databases

    Gene3Di3.30.730.10. 2 hits.
    InterProiIPR001471. AP2/ERF_dom.
    IPR016177. DNA-bd_dom.
    [Graphical view]
    PfamiPF00847. AP2. 2 hits.
    [Graphical view]
    PRINTSiPR00367. ETHRSPELEMNT.
    SMARTiSM00380. AP2. 2 hits.
    [Graphical view]
    SUPFAMiSSF54171. SSF54171. 2 hits.
    PROSITEiPS51032. AP2_ERF. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47927-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWDLNDAPHQ TQREEESEEF CYSSPSKRVG SFSNSSSSAV VIEDGSDDDE    50
    LNRVRPNNPL VTHQFFPEMD SNGGGVASGF PRAHWFGVKF CQSDLATGSS 100
    AGKATNVAAA VVEPAQPLKK SRRGPRSRSS QYRGVTFYRR TGRWESHIWD 150
    CGKQVYLGGF DTAHAAARAY DRAAIKFRGV EADINFNIDD YDDDLKQMTN 200
    LTKEEFVHVL RRQSTGFPRG SSKYRGVTLH KCGRWEARMG QFLGKKYVYL 250
    GLFDTEVEAA RAYDKAAIKC NGKDAVTNFD PSIYDEELNA ESSGNPTTPQ 300
    DHNLDLSLGN SANSKHKSQD MRLRMNQQQQ DSLHSNEVLG LGQTGMLNHT 350
    PNSNHQFPGS SNIGSGGGFS LFPAAENHRF DGRASTNQVL TNAAASSGFS 400
    PHHHNQIFNS TSTPHQNWLQ TNGFQPPLMR PS 432
    Length:432
    Mass (Da):47,833
    Last modified:February 1, 1996 - v1
    Checksum:i376A355291EFFB42
    GO

    Sequence cautioni

    The sequence AAM91531.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12546 mRNA. Translation: AAC13770.1.
    Z99707 Genomic DNA. Translation: CAB16765.1.
    AL161590 Genomic DNA. Translation: CAB80358.1.
    CP002687 Genomic DNA. Translation: AEE86717.1.
    CP002687 Genomic DNA. Translation: AEE86718.1.
    AY128328 mRNA. Translation: AAM91531.1. Different initiation.
    PIRiA85436.
    RefSeqiNP_001190938.1. NM_001204009.1.
    NP_195410.1. NM_119856.2.
    UniGeneiAt.4638.

    Genome annotation databases

    EnsemblPlantsiAT4G36920.1; AT4G36920.1; AT4G36920.
    AT4G36920.2; AT4G36920.2; AT4G36920.
    GeneIDi829845.
    KEGGiath:AT4G36920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12546 mRNA. Translation: AAC13770.1 .
    Z99707 Genomic DNA. Translation: CAB16765.1 .
    AL161590 Genomic DNA. Translation: CAB80358.1 .
    CP002687 Genomic DNA. Translation: AEE86717.1 .
    CP002687 Genomic DNA. Translation: AEE86718.1 .
    AY128328 mRNA. Translation: AAM91531.1 . Different initiation.
    PIRi A85436.
    RefSeqi NP_001190938.1. NM_001204009.1.
    NP_195410.1. NM_119856.2.
    UniGenei At.4638.

    3D structure databases

    ProteinModelPortali P47927.
    SMRi P47927. Positions 131-189, 223-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 15126. 4 interactions.
    STRINGi 3702.AT4G36920.1-P.

    Proteomic databases

    PRIDEi P47927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G36920.1 ; AT4G36920.1 ; AT4G36920 .
    AT4G36920.2 ; AT4G36920.2 ; AT4G36920 .
    GeneIDi 829845.
    KEGGi ath:AT4G36920.

    Organism-specific databases

    TAIRi AT4G36920.

    Phylogenomic databases

    eggNOGi NOG247476.
    HOGENOMi HOG000033977.
    InParanoidi P47927.
    KOi K09284.
    OMAi ESEEFCY.
    PhylomeDBi P47927.

    Gene expression databases

    Genevestigatori P47927.

    Family and domain databases

    Gene3Di 3.30.730.10. 2 hits.
    InterProi IPR001471. AP2/ERF_dom.
    IPR016177. DNA-bd_dom.
    [Graphical view ]
    Pfami PF00847. AP2. 2 hits.
    [Graphical view ]
    PRINTSi PR00367. ETHRSPELEMNT.
    SMARTi SM00380. AP2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF54171. SSF54171. 2 hits.
    PROSITEi PS51032. AP2_ERF. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Control of Arabidopsis flower and seed development by the homeotic gene APETALA2."
      Jofuku K.D., den Boer B.G.W., Van Montagu M., Okamuro J.K.
      Plant Cell 6:1211-1225(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANTS AP2-1 AND AP2-5.
      Strain: cv. Columbia.
      Tissue: Flower.
    2. "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
      Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.
      , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
      Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 221-432.
      Strain: cv. Columbia.
    6. "Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product."
      Drews G.N., Bowman J.L., Meyerowitz E.M.
      Cell 65:991-1002(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes."
      Aukerman M.J., Sakai H.
      Plant Cell 15:2730-2741(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis."
      Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.
      Plant Physiol. 132:1020-1032(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. "A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development."
      Chen X.
      Science 303:2022-2025(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
      Nakano T., Suzuki K., Fujimura T., Shinshi H.
      Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiAP2_ARATH
    AccessioniPrimary (citable) accession number: P47927
    Secondary accession number(s): Q8L7P9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mutations in the APETALA2 gene result in the ectopic expression of AGAMOUS, leading to the replacement of sepals by carpels and stamens and of petals by stamens.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3