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P47927

- AP2_ARATH

UniProt

P47927 - AP2_ARATH

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Protein
Floral homeotic protein APETALA 2
Gene
AP2, At4g36920, AP22.49, C7A10.440
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable transcriptional activator that promotes early floral meristem identity. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Plays a central role in the specification of floral identity, particularly for the normal development of sepals and petals in the wild-type flower. Acts as A class cadastral protein by repressing the C class floral homeotic gene AGAMOUS in association with other repressors like LEUNIG and SEUSS. It is also required during seed development.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi131 – 18757AP2/ERF 1
Add
BLAST
DNA bindingi223 – 28058AP2/ERF 2
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. flower development Source: TAIR
  3. meristem maintenance Source: TAIR
  4. ovule development Source: TAIR
  5. seed development Source: TAIR
  6. specification of floral organ identity Source: TAIR
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Floral homeotic protein APETALA 2
Gene namesi
Name:AP2
Ordered Locus Names:At4g36920
ORF Names:AP22.49, C7A10.440
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G36920.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi159 – 1591G → E in AP2-5; temperature-sensitive.
Mutagenesisi251 – 2511G → S in AP2-1; temperature-sensitive.
Mutagenesisi420 – 4201Q → E in AP2-5; temperature-sensitive.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Floral homeotic protein APETALA 2
PRO_0000112522Add
BLAST

Proteomic databases

PRIDEiP47927.

Expressioni

Tissue specificityi

Sepals, petals, stamens, carpels, developing ovules, inflorescence stem, leaf and stem.

Developmental stagei

It is detectable at low levels throughout the shoot apex and at enhanced levels in the inflorescence meristem, young floral buds and throughout the early stages of flower development and organogenesis. During floral organ differentiation it becomes spatially restricted to specific organ, tissue and cell types within the flower.

Inductioni

Negatively regulated by the C class floral homeotic protein AGAMOUS in stamens and carpels. MicroRNA 172 (miRNA172) negatively regulates APETALA2 at the translational level and may modulate its expression pattern. Seems not to be influenced by jasmonate and Alternaria brassicicola.3 Publications

Gene expression databases

GenevestigatoriP47927.

Interactioni

Subunit structurei

May form homodimer.

Protein-protein interaction databases

BioGridi15126. 4 interactions.
STRINGi3702.AT4G36920.1-P.

Structurei

3D structure databases

ProteinModelPortaliP47927.
SMRiP47927. Positions 131-189, 223-282.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi119 – 12810Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 5037Asp/Glu/Ser-rich (acidic; potential involvement with transcription)
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG247476.
HOGENOMiHOG000033977.
InParanoidiP47927.
KOiK09284.
OMAiESEEFCY.
PhylomeDBiP47927.

Family and domain databases

Gene3Di3.30.730.10. 2 hits.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 2 hits.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 2 hits.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 2 hits.
PROSITEiPS51032. AP2_ERF. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47927-1 [UniParc]FASTAAdd to Basket

« Hide

MWDLNDAPHQ TQREEESEEF CYSSPSKRVG SFSNSSSSAV VIEDGSDDDE    50
LNRVRPNNPL VTHQFFPEMD SNGGGVASGF PRAHWFGVKF CQSDLATGSS 100
AGKATNVAAA VVEPAQPLKK SRRGPRSRSS QYRGVTFYRR TGRWESHIWD 150
CGKQVYLGGF DTAHAAARAY DRAAIKFRGV EADINFNIDD YDDDLKQMTN 200
LTKEEFVHVL RRQSTGFPRG SSKYRGVTLH KCGRWEARMG QFLGKKYVYL 250
GLFDTEVEAA RAYDKAAIKC NGKDAVTNFD PSIYDEELNA ESSGNPTTPQ 300
DHNLDLSLGN SANSKHKSQD MRLRMNQQQQ DSLHSNEVLG LGQTGMLNHT 350
PNSNHQFPGS SNIGSGGGFS LFPAAENHRF DGRASTNQVL TNAAASSGFS 400
PHHHNQIFNS TSTPHQNWLQ TNGFQPPLMR PS 432
Length:432
Mass (Da):47,833
Last modified:February 1, 1996 - v1
Checksum:i376A355291EFFB42
GO

Sequence cautioni

The sequence AAM91531.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12546 mRNA. Translation: AAC13770.1.
Z99707 Genomic DNA. Translation: CAB16765.1.
AL161590 Genomic DNA. Translation: CAB80358.1.
CP002687 Genomic DNA. Translation: AEE86717.1.
CP002687 Genomic DNA. Translation: AEE86718.1.
AY128328 mRNA. Translation: AAM91531.1. Different initiation.
PIRiA85436.
RefSeqiNP_001190938.1. NM_001204009.1.
NP_195410.1. NM_119856.2.
UniGeneiAt.4638.

Genome annotation databases

EnsemblPlantsiAT4G36920.1; AT4G36920.1; AT4G36920.
AT4G36920.2; AT4G36920.2; AT4G36920.
GeneIDi829845.
KEGGiath:AT4G36920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12546 mRNA. Translation: AAC13770.1 .
Z99707 Genomic DNA. Translation: CAB16765.1 .
AL161590 Genomic DNA. Translation: CAB80358.1 .
CP002687 Genomic DNA. Translation: AEE86717.1 .
CP002687 Genomic DNA. Translation: AEE86718.1 .
AY128328 mRNA. Translation: AAM91531.1 . Different initiation.
PIRi A85436.
RefSeqi NP_001190938.1. NM_001204009.1.
NP_195410.1. NM_119856.2.
UniGenei At.4638.

3D structure databases

ProteinModelPortali P47927.
SMRi P47927. Positions 131-189, 223-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 15126. 4 interactions.
STRINGi 3702.AT4G36920.1-P.

Proteomic databases

PRIDEi P47927.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G36920.1 ; AT4G36920.1 ; AT4G36920 .
AT4G36920.2 ; AT4G36920.2 ; AT4G36920 .
GeneIDi 829845.
KEGGi ath:AT4G36920.

Organism-specific databases

TAIRi AT4G36920.

Phylogenomic databases

eggNOGi NOG247476.
HOGENOMi HOG000033977.
InParanoidi P47927.
KOi K09284.
OMAi ESEEFCY.
PhylomeDBi P47927.

Gene expression databases

Genevestigatori P47927.

Family and domain databases

Gene3Di 3.30.730.10. 2 hits.
InterProi IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view ]
Pfami PF00847. AP2. 2 hits.
[Graphical view ]
PRINTSi PR00367. ETHRSPELEMNT.
SMARTi SM00380. AP2. 2 hits.
[Graphical view ]
SUPFAMi SSF54171. SSF54171. 2 hits.
PROSITEi PS51032. AP2_ERF. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Control of Arabidopsis flower and seed development by the homeotic gene APETALA2."
    Jofuku K.D., den Boer B.G.W., Van Montagu M., Okamuro J.K.
    Plant Cell 6:1211-1225(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANTS AP2-1 AND AP2-5.
    Strain: cv. Columbia.
    Tissue: Flower.
  2. "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
    Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.
    , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
    Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 221-432.
    Strain: cv. Columbia.
  6. "Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product."
    Drews G.N., Bowman J.L., Meyerowitz E.M.
    Cell 65:991-1002(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes."
    Aukerman M.J., Sakai H.
    Plant Cell 15:2730-2741(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis."
    Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.
    Plant Physiol. 132:1020-1032(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development."
    Chen X.
    Science 303:2022-2025(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
    Nakano T., Suzuki K., Fujimura T., Shinshi H.
    Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiAP2_ARATH
AccessioniPrimary (citable) accession number: P47927
Secondary accession number(s): Q8L7P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutations in the APETALA2 gene result in the ectopic expression of AGAMOUS, leading to the replacement of sepals by carpels and stamens and of petals by stamens.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi