P47923 (NDK2_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase 2, chloroplastic
EC=2.7.4.6

Alternative name(s):
Nucleoside diphosphate kinase II
Short name=NDK II
Short name=NDP kinase II
Short name=NDPK II

Cleaved into the following 2 chains:

1. Nucleoside diphosphate kinase 2 high molecular weight
2. Nucleoside diphosphate kinase 2 low molecular weight

Gene names

Name:

NDPK2

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 64	64	Chloroplast Ref.1
	Chain	65 – 230	166	Nucleoside diphosphate kinase 2 high molecular weight		PRO_0000019437
	Chain	78 – 230	153	Nucleoside diphosphate kinase 2 low molecular weight		PRO_0000019438
Sites
	Active site	196	1	Pros-phosphohistidine intermediate By similarity
	Binding site	90	1	ATP By similarity
	Binding site	138	1	ATP By similarity
	Binding site	166	1	ATP By similarity
	Binding site	172	1	ATP By similarity
	Binding site	183	1	ATP By similarity
	Binding site	193	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47923 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A5A10604D9E6D505	FASTA	230	25,617
	10 20 30 40 50 60 MEAMAVFSGS NLFATSSLLL TTNSKTRYSQ LRTTQNLSAF SSKSHLFSPS STSSSYPKTF 70 80 90 100 110 120 RTRSSTESGI FLPRLITSLE QVDQAYIMVK PDGVQRGLVG EIISRFEKKG FKLTGLKLFQ 130 140 150 160 170 180 CSKELAEEHY KHLNQKSFFP KLIEYITSGP VVSMAWEGVG VVPSARKLIG ATDPLQAEPG 190 200 210 220 230 TIRGDFAVQT GRNIIHGSDS PENGEREIAL WFKEGELCEW TPVQEPWLRE

References

[1]	"Nucleoside diphosphate kinase from pea chloroplasts: purification, cDNA cloning and import into chloroplasts." Luebeck J., Soll J. Planta 196:668-673(1995) [PubMed: 7580854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 65-76 AND 78-107. Strain: cv. Golf. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z37990 mRNA. Translation: CAA86071.1.
PIR	S52785.
3D structure databases
ProteinModelPortal	P47923.
SMR	P47923. Positions 78-230.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK2_PEA

Accession

Primary (citable) accession number: P47923

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program