P47922 (NDK1_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase 1
EC=2.7.4.6

Alternative name(s):
Nucleoside diphosphate kinase I
Short name=NDK I
Short name=NDP kinase I
Short name=NDPK I

Gene names

Name:	NDPK1
Synonyms:	NDKN1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. This NDK is microtubule-associated.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism Phytochrome signaling pathway
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro red, far-red light phototransduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Nucleoside diphosphate kinase 1		PRO_0000137142
Sites
	Active site	116	1	Pros-phosphohistidine intermediate By similarity
	Binding site	10	1	ATP By similarity
	Binding site	58	1	ATP By similarity
	Binding site	86	1	ATP By similarity
	Binding site	92	1	ATP By similarity
	Binding site	103	1	ATP By similarity
	Binding site	113	1	ATP By similarity
Experimental info
	Sequence conflict	140	1	E → Q in BAA12982. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47922 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7F1E17795BE598C0	FASTA	149	16,463
	10 20 30 40 50 60 MAEQTFIMIK PDGVQRGLVG EIISRFEKKG FYLKGLKFVN VERAFAEKHY ADLSAKPFFS 70 80 90 100 110 120 GLVDYIISGP VVAMIWEGKN VVTTGRKIIG ATNPAQSEPG TIRGDFAIDI GRNVIHGSDA 130 140 VESANKEIAL WFPEGAANWE SSLHSWIYE

References

[1]	"Molecular cloning, sequence determination and heterologous expression of nucleoside diphosphate kinase from Pisum sativum." Finan P., White I.R., Redpath S.H., Findlay J.B.C., Millner P.A. Plant Mol. Biol. 25:59-67(1994) [PubMed: 8003697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Feltham First. Tissue: Seed.
[2]	"Phytochrome-mediated light signals are transduced to nucleoside diphosphate kinase in Pisum sativum L. cv. Alaska." Tanaka N., Ogura T., Noguchi T., Hirano H., Yabe N., Hasunuma K. J. Photochem. Photobiol. B 45:113-121(1998) [PubMed: 9868801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Alaska.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71388 mRNA. Translation: CAA50511.1. D86052 mRNA. Translation: BAA12982.1.
PIR	S33170. S46513.
3D structure databases
ProteinModelPortal	P47922.
SMR	P47922. Positions 3-149.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK1_PEA

Accession

Primary (citable) accession number: P47922
Secondary accession number(s): Q41047

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program