Reviewed, UniProtKB/Swiss-Prot P47920 (NDKB_FLABI)

Names and origin

Protein names	Recommended name:     Nucleoside diphosphate kinase B       Short name=NDP kinase B       Short name=NDK B     EC=2.7.4.6
Organism	Flaveria bidentis (Coastalplain yellowtops)
Taxonomic identifier	4224 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Tageteae › Flaveria

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Nucleoside diphosphate kinase B		PRO_0000137137
Sites
	Active site	115	1	Pros-phosphohistidine intermediate By similarity
	Binding site	9	1	ATP By similarity
	Binding site	57	1	ATP By similarity
	Binding site	85	1	ATP By similarity
	Binding site	91	1	ATP By similarity
	Binding site	102	1	ATP By similarity
	Binding site	112	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47920-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2BE3158FFACBA3C0	FASTA	148	16,200
	10 20 30 40 50 60 MEHTFIMIKP DGVQRGLVGE IIGRFEKKGF SLKGLKLLTV DQAFAEKHYA DLSAKPFFNG 70 80 90 100 110 120 LVEYIISGPV VAMVWEGKNV VTTGRKIIGA TNPAESAPGT IRGDFAIDIG RNVIHGSDAV 130 140 ESAKKEIALW FPEGVANWSS SLHPWIYE

References

[1]	"Molecular and biochemical characterization of two nucleoside diphosphate kinase cDNA clones from Flaveria bidentis." Ananvoranich S., Grandmaison J., Gulick P.J. Genome 39:404-409(1996) [PubMed: 8984006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10283 mRNA. Translation: AAA19005.1.
3D structure databases
HSSP	HSSP built from PDB template 1NSQ based on UniProtKB P08879.
SMR	P47920. Positions 1-148.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.4.6. 190363.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKB_FLABI

Accession

Primary (citable) accession number: P47920

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)