Reviewed, UniProtKB/Swiss-Prot P47912 (LCF4_YEAST)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase 4
    EC=6.2.1.3
Alternative name(s):
    Long-chain acyl-CoA synthetase 4
    Fatty acid activator 4

Gene names

Name:	FAA4
Ordered Locus Names:	YMR246W
ORF Names:	YM9408.08

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Contributes, with FAA1, to the activation of imported myristate.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium By similarity.
Miscellaneous	Present with 31200 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Ref.1 Inferred from genetic interaction. Source: SGD fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid transport Inferred from genetic interaction. Source: SGD
Cellular component	lipid particle Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Inferred from direct assay. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 694	694	Long-chain-fatty-acid--CoA ligase 4		PRO_0000193122
Experimental info
	Sequence conflict	552	1	N → G in CAA88635. Ref.1
	Sequence conflict	631	1	V → G in CAA88635. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47912-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CF6524949C96D24D	FASTA	694	77,267
	10 20 30 40 50 60 MTEQYSVAVG EAANEHETAP RRNIRVKDQP LIRPINSSAS TLYEFALECF TKGGKRDGMA 70 80 90 100 110 120 WRDIIDIHET KKTIVKRVDG KDKPIEKTWL YYELTPYITM TYEEMICVMH DIGRGLIKIG 130 140 150 160 170 180 VKPNGENKFH IFASTSHKWM KTFLGCMSQG IPVVTAYDTL GESGLIHSMV ETDSVAIFTD 190 200 210 220 230 240 NQLLSKLAVP LKTAKNVKFV IHNEPIDPSD KRQNGKLYKA AKDAVDKIKE VRPDIKIYSF 250 260 270 280 290 300 DEIIEIGKKA KDEVELHFPK PEDPACIMYT SGSTGTPKGV VLTHYNIVAG IGGVGHNVIG 310 320 330 340 350 360 WIGPTDRIIA FLPLAHIFEL TFEFEAFYWN GILGYANVKT LTPTSTRNCQ GDLMEFKPTV 370 380 390 400 410 420 MVGVAAVWET VRKGILAKIN ELPGWSQTLF WTVYALKERN IPCSGLLSGL IFKRIREATG 430 440 450 460 470 480 GNLRFILNGG SAISIDAQKF LSNLLCPMLI GYGLTEGVAN ACVLEPEHFD YGIAGDLVGT 490 500 510 520 530 540 ITAKLVDVED LGYFAKNNQG ELLFKGAPIC SEYYKNPEET AAAFTDDGWF RTGDIAEWTP 550 560 570 580 590 600 KGQVKIIDRK KNLVKTLNGE YIALEKLESI YRSNPYVQNI CVYADENKVK PVGIVVPNLG 610 620 630 640 650 660 HLSKLAIELG IMVPGEDVES YIHEKKLQDA VCKDMLSTAK SQGLNGIELL CGIVFFEEEW 670 680 690 TPENGLVTSA QKLKRRDILA AVKPDVERVY KENT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae contains four fatty acid activation (FAA) genes: an assessment of their role in regulating protein N-myristoylation and cellular lipid metabolism." Johnson D.R., Knoll L.J., Levin D.E., Gordon J.I. J. Cell Biol. 127:751-762(1994) [PubMed: 7962057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48744 Genomic DNA. Translation: CAA88635.1. Z48756 Genomic DNA. Translation: CAA88656.1.
PIR	S56060.
RefSeq	NP_013974.1.
3D structure databases
SMR	P47912. Positions 99-683.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4361N.
IntAct	P47912. 27 interactions.
STRING	P47912.
Proteomic databases
PeptideAtlas	P47912.
PRIDE	P47912.
Genome annotation databases
Ensembl	YMR246W; YMR246W; YMR246W; Saccharomyces cerevisiae. [Genome view]
GeneID	855288.
KEGG	sce:YMR246W.
NMPDR	fig|4932.3.peg.5029.
Organism-specific databases
CYGD	YMR246w.
SGD	S000004860. FAA4.
Phylogenomic databases
eggNOG	fuNOG04652.
HOGENOM	HBG721674.
OMA	VMVGVAA.
OrthoDB	EOG9JM95Z.
PhylomeDB	P47912.
Enzyme and pathway databases
BRENDA	6.2.1.3. 250.
Gene expression databases
ArrayExpress	P47912.
Genevestigator	P47912.
GermOnline	YMR246W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978932.

Entry information

Entry name

LCF4_YEAST

Accession

Primary (citable) accession number: P47912
Secondary accession number(s): Q05743

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 19, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)