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P47912

- LCF4_YEAST

UniProt

P47912 - LCF4_YEAST

Protein

Long-chain-fatty-acid--CoA ligase 4

Gene

FAA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Contributes, with FAA1, to the activation of imported myristate.

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: SGD

    GO - Biological processi

    1. long-chain fatty acid metabolic process Source: GOC
    2. long-chain fatty acid transport Source: SGD
    3. long-chain fatty-acyl-CoA metabolic process Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YMR246W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-chain-fatty-acid--CoA ligase 4 (EC:6.2.1.3)
    Alternative name(s):
    Fatty acid activator 4
    Long-chain acyl-CoA synthetase 4
    Gene namesi
    Name:FAA4
    Ordered Locus Names:YMR246W
    ORF Names:YM9408.08
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR246w.
    SGDiS000004860. FAA4.

    Subcellular locationi

    GO - Cellular componenti

    1. lipid particle Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 694694Long-chain-fatty-acid--CoA ligase 4PRO_0000193122Add
    BLAST

    Proteomic databases

    MaxQBiP47912.
    PaxDbiP47912.
    PeptideAtlasiP47912.
    PRIDEiP47912.

    Expressioni

    Gene expression databases

    GenevestigatoriP47912.

    Interactioni

    Protein-protein interaction databases

    BioGridi35425. 64 interactions.
    DIPiDIP-4361N.
    IntActiP47912. 29 interactions.
    MINTiMINT-516514.
    STRINGi4932.YMR246W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47912.
    SMRiP47912. Positions 101-683.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    GeneTreeiENSGT00690000102168.
    HOGENOMiHOG000159459.
    KOiK01897.
    OMAiGYVTSAQ.
    OrthoDBiEOG7CCC0K.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47912-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEQYSVAVG EAANEHETAP RRNIRVKDQP LIRPINSSAS TLYEFALECF    50
    TKGGKRDGMA WRDIIDIHET KKTIVKRVDG KDKPIEKTWL YYELTPYITM 100
    TYEEMICVMH DIGRGLIKIG VKPNGENKFH IFASTSHKWM KTFLGCMSQG 150
    IPVVTAYDTL GESGLIHSMV ETDSVAIFTD NQLLSKLAVP LKTAKNVKFV 200
    IHNEPIDPSD KRQNGKLYKA AKDAVDKIKE VRPDIKIYSF DEIIEIGKKA 250
    KDEVELHFPK PEDPACIMYT SGSTGTPKGV VLTHYNIVAG IGGVGHNVIG 300
    WIGPTDRIIA FLPLAHIFEL TFEFEAFYWN GILGYANVKT LTPTSTRNCQ 350
    GDLMEFKPTV MVGVAAVWET VRKGILAKIN ELPGWSQTLF WTVYALKERN 400
    IPCSGLLSGL IFKRIREATG GNLRFILNGG SAISIDAQKF LSNLLCPMLI 450
    GYGLTEGVAN ACVLEPEHFD YGIAGDLVGT ITAKLVDVED LGYFAKNNQG 500
    ELLFKGAPIC SEYYKNPEET AAAFTDDGWF RTGDIAEWTP KGQVKIIDRK 550
    KNLVKTLNGE YIALEKLESI YRSNPYVQNI CVYADENKVK PVGIVVPNLG 600
    HLSKLAIELG IMVPGEDVES YIHEKKLQDA VCKDMLSTAK SQGLNGIELL 650
    CGIVFFEEEW TPENGLVTSA QKLKRRDILA AVKPDVERVY KENT 694
    Length:694
    Mass (Da):77,267
    Last modified:February 1, 1996 - v1
    Checksum:iCF6524949C96D24D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti552 – 5521N → G in CAA88635. (PubMed:7962057)Curated
    Sequence conflicti631 – 6311V → G in CAA88635. (PubMed:7962057)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48744 Genomic DNA. Translation: CAA88635.1.
    Z48756 Genomic DNA. Translation: CAA88656.1.
    BK006946 Genomic DNA. Translation: DAA10147.1.
    PIRiS56060.
    RefSeqiNP_013974.1. NM_001182754.1.

    Genome annotation databases

    EnsemblFungiiYMR246W; YMR246W; YMR246W.
    GeneIDi855288.
    KEGGisce:YMR246W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48744 Genomic DNA. Translation: CAA88635.1 .
    Z48756 Genomic DNA. Translation: CAA88656.1 .
    BK006946 Genomic DNA. Translation: DAA10147.1 .
    PIRi S56060.
    RefSeqi NP_013974.1. NM_001182754.1.

    3D structure databases

    ProteinModelPortali P47912.
    SMRi P47912. Positions 101-683.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35425. 64 interactions.
    DIPi DIP-4361N.
    IntActi P47912. 29 interactions.
    MINTi MINT-516514.
    STRINGi 4932.YMR246W.

    Proteomic databases

    MaxQBi P47912.
    PaxDbi P47912.
    PeptideAtlasi P47912.
    PRIDEi P47912.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR246W ; YMR246W ; YMR246W .
    GeneIDi 855288.
    KEGGi sce:YMR246W.

    Organism-specific databases

    CYGDi YMR246w.
    SGDi S000004860. FAA4.

    Phylogenomic databases

    eggNOGi COG1022.
    GeneTreei ENSGT00690000102168.
    HOGENOMi HOG000159459.
    KOi K01897.
    OMAi GYVTSAQ.
    OrthoDBi EOG7CCC0K.

    Enzyme and pathway databases

    BioCyci YEAST:YMR246W-MONOMER.

    Miscellaneous databases

    NextBioi 978932.

    Gene expression databases

    Genevestigatori P47912.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Saccharomyces cerevisiae contains four fatty acid activation (FAA) genes: an assessment of their role in regulating protein N-myristoylation and cellular lipid metabolism."
      Johnson D.R., Knoll L.J., Levin D.E., Gordon J.I.
      J. Cell Biol. 127:751-762(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLCF4_YEAST
    AccessioniPrimary (citable) accession number: P47912
    Secondary accession number(s): D6W073, Q05743
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 31200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3