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P47912

- LCF4_YEAST

UniProt

P47912 - LCF4_YEAST

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Protein

Long-chain-fatty-acid--CoA ligase 4

Gene

FAA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Contributes, with FAA1, to the activation of imported myristate.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: SGD

GO - Biological processi

  1. long-chain fatty acid metabolic process Source: GOC
  2. long-chain fatty acid transport Source: SGD
  3. long-chain fatty-acyl-CoA metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YMR246W-MONOMER.
ReactomeiREACT_239919. Synthesis of very long-chain fatty acyl-CoAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 4 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 4
Long-chain acyl-CoA synthetase 4
Gene namesi
Name:FAA4
Ordered Locus Names:YMR246W
ORF Names:YM9408.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR246w.
SGDiS000004860. FAA4.

Subcellular locationi

GO - Cellular componenti

  1. lipid particle Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 694694Long-chain-fatty-acid--CoA ligase 4PRO_0000193122Add
BLAST

Proteomic databases

MaxQBiP47912.
PaxDbiP47912.
PeptideAtlasiP47912.
PRIDEiP47912.

Expressioni

Gene expression databases

GenevestigatoriP47912.

Interactioni

Protein-protein interaction databases

BioGridi35425. 65 interactions.
DIPiDIP-4361N.
IntActiP47912. 29 interactions.
MINTiMINT-516514.
STRINGi4932.YMR246W.

Structurei

3D structure databases

ProteinModelPortaliP47912.
SMRiP47912. Positions 101-683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000102168.
HOGENOMiHOG000159459.
InParanoidiP47912.
KOiK01897.
OMAiGYVTSAQ.
OrthoDBiEOG7CCC0K.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47912-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEQYSVAVG EAANEHETAP RRNIRVKDQP LIRPINSSAS TLYEFALECF
60 70 80 90 100
TKGGKRDGMA WRDIIDIHET KKTIVKRVDG KDKPIEKTWL YYELTPYITM
110 120 130 140 150
TYEEMICVMH DIGRGLIKIG VKPNGENKFH IFASTSHKWM KTFLGCMSQG
160 170 180 190 200
IPVVTAYDTL GESGLIHSMV ETDSVAIFTD NQLLSKLAVP LKTAKNVKFV
210 220 230 240 250
IHNEPIDPSD KRQNGKLYKA AKDAVDKIKE VRPDIKIYSF DEIIEIGKKA
260 270 280 290 300
KDEVELHFPK PEDPACIMYT SGSTGTPKGV VLTHYNIVAG IGGVGHNVIG
310 320 330 340 350
WIGPTDRIIA FLPLAHIFEL TFEFEAFYWN GILGYANVKT LTPTSTRNCQ
360 370 380 390 400
GDLMEFKPTV MVGVAAVWET VRKGILAKIN ELPGWSQTLF WTVYALKERN
410 420 430 440 450
IPCSGLLSGL IFKRIREATG GNLRFILNGG SAISIDAQKF LSNLLCPMLI
460 470 480 490 500
GYGLTEGVAN ACVLEPEHFD YGIAGDLVGT ITAKLVDVED LGYFAKNNQG
510 520 530 540 550
ELLFKGAPIC SEYYKNPEET AAAFTDDGWF RTGDIAEWTP KGQVKIIDRK
560 570 580 590 600
KNLVKTLNGE YIALEKLESI YRSNPYVQNI CVYADENKVK PVGIVVPNLG
610 620 630 640 650
HLSKLAIELG IMVPGEDVES YIHEKKLQDA VCKDMLSTAK SQGLNGIELL
660 670 680 690
CGIVFFEEEW TPENGLVTSA QKLKRRDILA AVKPDVERVY KENT
Length:694
Mass (Da):77,267
Last modified:February 1, 1996 - v1
Checksum:iCF6524949C96D24D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti552 – 5521N → G in CAA88635. (PubMed:7962057)Curated
Sequence conflicti631 – 6311V → G in CAA88635. (PubMed:7962057)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48744 Genomic DNA. Translation: CAA88635.1.
Z48756 Genomic DNA. Translation: CAA88656.1.
BK006946 Genomic DNA. Translation: DAA10147.1.
PIRiS56060.
RefSeqiNP_013974.1. NM_001182754.1.

Genome annotation databases

EnsemblFungiiYMR246W; YMR246W; YMR246W.
GeneIDi855288.
KEGGisce:YMR246W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48744 Genomic DNA. Translation: CAA88635.1 .
Z48756 Genomic DNA. Translation: CAA88656.1 .
BK006946 Genomic DNA. Translation: DAA10147.1 .
PIRi S56060.
RefSeqi NP_013974.1. NM_001182754.1.

3D structure databases

ProteinModelPortali P47912.
SMRi P47912. Positions 101-683.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35425. 65 interactions.
DIPi DIP-4361N.
IntActi P47912. 29 interactions.
MINTi MINT-516514.
STRINGi 4932.YMR246W.

Proteomic databases

MaxQBi P47912.
PaxDbi P47912.
PeptideAtlasi P47912.
PRIDEi P47912.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR246W ; YMR246W ; YMR246W .
GeneIDi 855288.
KEGGi sce:YMR246W.

Organism-specific databases

CYGDi YMR246w.
SGDi S000004860. FAA4.

Phylogenomic databases

eggNOGi COG1022.
GeneTreei ENSGT00690000102168.
HOGENOMi HOG000159459.
InParanoidi P47912.
KOi K01897.
OMAi GYVTSAQ.
OrthoDBi EOG7CCC0K.

Enzyme and pathway databases

BioCyci YEAST:YMR246W-MONOMER.
Reactomei REACT_239919. Synthesis of very long-chain fatty acyl-CoAs.

Miscellaneous databases

NextBioi 978932.

Gene expression databases

Genevestigatori P47912.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Saccharomyces cerevisiae contains four fatty acid activation (FAA) genes: an assessment of their role in regulating protein N-myristoylation and cellular lipid metabolism."
    Johnson D.R., Knoll L.J., Levin D.E., Gordon J.I.
    J. Cell Biol. 127:751-762(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLCF4_YEAST
AccessioniPrimary (citable) accession number: P47912
Secondary accession number(s): D6W073, Q05743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 31200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3