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Protein

Long-chain-fatty-acid--CoA ligase 4

Gene

FAA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Contributes, with FAA1, to the activation of imported myristate.

Miscellaneous

Present with 31200 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

  • long-chain fatty acid import Source: SGD
  • long-chain fatty-acyl-CoA metabolic process Source: SGD
  • sphingoid long-chain base transport Source: SGD

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YMR246W-MONOMER
ReactomeiR-SCE-75876 Synthesis of very long-chain fatty acyl-CoAs

Chemistry databases

SwissLipidsiSLP:000000130

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 4 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 4
Long-chain acyl-CoA synthetase 4
Gene namesi
Name:FAA4
Ordered Locus Names:YMR246W
ORF Names:YM9408.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR246W
SGDiS000004860 FAA4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931221 – 694Long-chain-fatty-acid--CoA ligase 4Add BLAST694

Proteomic databases

MaxQBiP47912
PaxDbiP47912
PRIDEiP47912

Interactioni

Protein-protein interaction databases

BioGridi35425111 interactors.
DIPiDIP-4361N
IntActiP47912 45 interactors.
MINTiP47912
STRINGi4932.YMR246W

Structurei

3D structure databases

ProteinModelPortaliP47912
SMRiP47912
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00870000136474
HOGENOMiHOG000159459
InParanoidiP47912
KOiK01897
OMAiSVLPIWH
OrthoDBiEOG092C0RYI

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P47912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQYSVAVG EAANEHETAP RRNIRVKDQP LIRPINSSAS TLYEFALECF
60 70 80 90 100
TKGGKRDGMA WRDIIDIHET KKTIVKRVDG KDKPIEKTWL YYELTPYITM
110 120 130 140 150
TYEEMICVMH DIGRGLIKIG VKPNGENKFH IFASTSHKWM KTFLGCMSQG
160 170 180 190 200
IPVVTAYDTL GESGLIHSMV ETDSVAIFTD NQLLSKLAVP LKTAKNVKFV
210 220 230 240 250
IHNEPIDPSD KRQNGKLYKA AKDAVDKIKE VRPDIKIYSF DEIIEIGKKA
260 270 280 290 300
KDEVELHFPK PEDPACIMYT SGSTGTPKGV VLTHYNIVAG IGGVGHNVIG
310 320 330 340 350
WIGPTDRIIA FLPLAHIFEL TFEFEAFYWN GILGYANVKT LTPTSTRNCQ
360 370 380 390 400
GDLMEFKPTV MVGVAAVWET VRKGILAKIN ELPGWSQTLF WTVYALKERN
410 420 430 440 450
IPCSGLLSGL IFKRIREATG GNLRFILNGG SAISIDAQKF LSNLLCPMLI
460 470 480 490 500
GYGLTEGVAN ACVLEPEHFD YGIAGDLVGT ITAKLVDVED LGYFAKNNQG
510 520 530 540 550
ELLFKGAPIC SEYYKNPEET AAAFTDDGWF RTGDIAEWTP KGQVKIIDRK
560 570 580 590 600
KNLVKTLNGE YIALEKLESI YRSNPYVQNI CVYADENKVK PVGIVVPNLG
610 620 630 640 650
HLSKLAIELG IMVPGEDVES YIHEKKLQDA VCKDMLSTAK SQGLNGIELL
660 670 680 690
CGIVFFEEEW TPENGLVTSA QKLKRRDILA AVKPDVERVY KENT
Length:694
Mass (Da):77,267
Last modified:February 1, 1996 - v1
Checksum:iCF6524949C96D24D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti552N → G in CAA88635 (PubMed:7962057).Curated1
Sequence conflicti631V → G in CAA88635 (PubMed:7962057).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48744 Genomic DNA Translation: CAA88635.1
Z48756 Genomic DNA Translation: CAA88656.1
BK006946 Genomic DNA Translation: DAA10147.1
PIRiS56060
RefSeqiNP_013974.1, NM_001182754.1

Genome annotation databases

EnsemblFungiiYMR246W; YMR246W; YMR246W
GeneIDi855288
KEGGisce:YMR246W

Similar proteinsi

Entry informationi

Entry nameiLCF4_YEAST
AccessioniPrimary (citable) accession number: P47912
Secondary accession number(s): D6W073, Q05743
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 25, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome