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Protein

Ribosome hibernation promotion factor

Gene

hpf

Organism
Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Might modulate either transcription and/or translation.1 Publication
Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth.UniRule annotation

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome hibernation promotion factorUniRule annotation
Short name:
HPFUniRule annotation
Alternative name(s):
Dark-response protein 211 Publication
Short name:
DRP-211 Publication
Light-repressed protein A1 Publication
Gene namesi
Name:hpfUniRule annotation
Synonyms:lrtA1 Publication
Ordered Locus Names:SYNPCC7002_A0263
OrganismiSynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Taxonomic identifieri32049 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001688 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Knockout mutants grown in continuous light appear to be the same as wild-type, showing no differences in growth or photosynthetic activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085871 – 193Ribosome hibernation promotion factorAdd BLAST193

Expressioni

Inductioni

Is repressed by light, since the transcript is synthesized in the dark, but upon 20 minutes of illumination, transcript levels fall below detectable limits. This light-repressed transcript is present only in dark-adapted cells. Is rapidly translated at the onset of illumination; in fact, is one of the first proteins to be synthesized upon illumination of dark-adapted cells. Repression of the transcript in the light requires protein synthesis, which suggests that a specific protein is directly involved in lrtA light repression.2 Publications

Interactioni

Subunit structurei

Interacts with 100S ribosomes.UniRule annotation

Protein-protein interaction databases

STRINGi32049.SYNPCC7002_A0263.

Structurei

3D structure databases

ProteinModelPortaliP47908.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105MS2. Bacteria.
COG1544. LUCA.
HOGENOMiHOG000074929.
KOiK05808.
OMAiGWTYGVI.
OrthoDBiPOG091H00J6.

Family and domain databases

CDDicd00552. RaiA. 1 hit.
Gene3Di3.30.160.100. 1 hit.
HAMAPiMF_00839. HPF. 1 hit.
InterProiIPR032528. Ribosom_S30AE_C.
IPR003489. Ribosomal_S30Ae/sigma54_mod.
[Graphical view]
PfamiPF16321. Ribosom_S30AE_C. 1 hit.
PF02482. Ribosomal_S30AE. 1 hit.
[Graphical view]
SUPFAMiSSF69754. SSF69754. 1 hit.
TIGRFAMsiTIGR00741. yfiA. 1 hit.

Sequencei

Sequence statusi: Complete.

P47908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLIQGNNI AVTESIHDYV ESKLEKATKH FQTFATKVDV HLSVANNARI
60 70 80 90 100
TDKHKAEVTV YANGTVIRAQ EGSENLYASI DLVSDKIARQ LRKYKEKNFG
110 120 130 140 150
KKTHVQEKTS EVLPEDPVPD NLIGDRAPEL PSEVVRMKYF AMPPMTIDEA
160 170 180 190
LEQLQLVDHD FYMFLNKDTN AINVIYIRNH GGYGVIQPRL GKE
Length:193
Mass (Da):21,911
Last modified:June 10, 2008 - v2
Checksum:i6521B45874951D98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122 – 131LIGDRAPELP → SLRSRPRAS in AAA62586 (PubMed:8063707).Curated10
Sequence conflicti149E → Q in AAA62586 (PubMed:8063707).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29194 Genomic DNA. Translation: AAA62586.1.
CP000951 Genomic DNA. Translation: ACA98273.1.
PIRiA53914.
RefSeqiWP_012305897.1. NC_010475.1.

Genome annotation databases

EnsemblBacteriaiACA98273; ACA98273; SYNPCC7002_A0263.
KEGGisyp:SYNPCC7002_A0263.
PATRICi23814962. VBISynSp37135_0444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29194 Genomic DNA. Translation: AAA62586.1.
CP000951 Genomic DNA. Translation: ACA98273.1.
PIRiA53914.
RefSeqiWP_012305897.1. NC_010475.1.

3D structure databases

ProteinModelPortaliP47908.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi32049.SYNPCC7002_A0263.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA98273; ACA98273; SYNPCC7002_A0263.
KEGGisyp:SYNPCC7002_A0263.
PATRICi23814962. VBISynSp37135_0444.

Phylogenomic databases

eggNOGiENOG4105MS2. Bacteria.
COG1544. LUCA.
HOGENOMiHOG000074929.
KOiK05808.
OMAiGWTYGVI.
OrthoDBiPOG091H00J6.

Family and domain databases

CDDicd00552. RaiA. 1 hit.
Gene3Di3.30.160.100. 1 hit.
HAMAPiMF_00839. HPF. 1 hit.
InterProiIPR032528. Ribosom_S30AE_C.
IPR003489. Ribosomal_S30Ae/sigma54_mod.
[Graphical view]
PfamiPF16321. Ribosom_S30AE_C. 1 hit.
PF02482. Ribosomal_S30AE. 1 hit.
[Graphical view]
SUPFAMiSSF69754. SSF69754. 1 hit.
TIGRFAMsiTIGR00741. yfiA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPF_SYNP2
AccessioniPrimary (citable) accession number: P47908
Secondary accession number(s): B1XMT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: June 10, 2008
Last modified: November 2, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The transcript for lrtA is more stable in the dark than in the light: a 3-hour half-life is estimated in the dark, whereas a 4-minute half-life is observed in the light.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.