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P47900 (P2RY1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
P2Y purinoceptor 1

Short name=P2Y1
Alternative name(s):
ATP receptor
Purinergic receptor
Gene names
Name:P2RY1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for extracellular adenine nucleotides such as ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein.

Induction

Repressed by the P2Y1 receptor-specific antagonists A3P5PS, A3P5P and A2P5P. These inhibit calcium ion mobilization and shape change in platelets.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processBlood coagulation
Hemostasis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

aging

Inferred from electronic annotation. Source: Ensembl

blood coagulation

Traceable author statement. Source: Reactome

brain development

Inferred from electronic annotation. Source: Ensembl

cell surface receptor signaling pathway

Traceable author statement Ref.3. Source: ProtInc

eating behavior

Inferred from electronic annotation. Source: Ensembl

negative regulation of binding

Inferred from electronic annotation. Source: Ensembl

negative regulation of norepinephrine secretion

Inferred from electronic annotation. Source: Ensembl

phospholipase C-activating G-protein coupled receptor signaling pathway

Traceable author statement Ref.3. Source: ProtInc

platelet activation

Traceable author statement. Source: Reactome

positive regulation of ERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of cytosolic calcium ion concentration

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of hormone secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of inositol trisphosphate biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of ion transport

Inferred from electronic annotation. Source: Ensembl

positive regulation of penile erection

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein phosphorylation

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

protein localization to plasma membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of receptor activity

Inferred from electronic annotation. Source: Ensembl

regulation of vasodilation

Inferred from electronic annotation. Source: Ensembl

relaxation of muscle

Inferred from electronic annotation. Source: InterPro

response to growth factor

Inferred from electronic annotation. Source: Ensembl

response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

signal transduction involved in regulation of gene expression

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: Ensembl

basolateral plasma membrane

Inferred from electronic annotation. Source: Ensembl

cell body

Inferred from sequence or structural similarity. Source: BHF-UCL

dendrite

Inferred from sequence or structural similarity. Source: BHF-UCL

integral component of plasma membrane

Traceable author statement Ref.3. Source: ProtInc

neuronal postsynaptic density

Inferred from sequence or structural similarity. Source: BHF-UCL

plasma membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

postsynaptic membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

   Molecular_functionADP binding

Inferred from electronic annotation. Source: Ensembl

ADP-activated nucleotide receptor activity

Inferred from electronic annotation. Source: Ensembl

ATP binding

Inferred from electronic annotation. Source: Ensembl

ATP-activated nucleotide receptor activity

Inferred from electronic annotation. Source: Ensembl

G-protein coupled purinergic nucleotide receptor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

receptor activity

Traceable author statement Ref.3. Source: ProtInc

scaffold protein binding

Inferred from sequence or structural similarity. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SLC9A3R1O147452EBI-8677223,EBI-349787

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373P2Y purinoceptor 1
PRO_0000070006

Regions

Topological domain1 – 5252Extracellular Potential
Transmembrane53 – 7422Helical; Name=1; Potential
Topological domain75 – 8713Cytoplasmic Potential
Transmembrane88 – 10922Helical; Name=2; Potential
Topological domain110 – 12617Extracellular Potential
Transmembrane127 – 14721Helical; Name=3; Potential
Topological domain148 – 16619Cytoplasmic Potential
Transmembrane167 – 18822Helical; Name=4; Potential
Topological domain189 – 21830Extracellular Potential
Transmembrane219 – 23820Helical; Name=5; Potential
Topological domain239 – 26527Cytoplasmic Potential
Transmembrane266 – 28520Helical; Name=6; Potential
Topological domain286 – 30318Extracellular Potential
Transmembrane304 – 32825Helical; Name=7; Potential
Topological domain329 – 37345Cytoplasmic Potential

Amino acid modifications

Glycosylation111N-linked (GlcNAc...) Potential
Glycosylation271N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1971N-linked (GlcNAc...) Potential
Disulfide bond124 ↔ 202 By similarity

Experimental info

Sequence conflict1381Missing in CAA89066. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P47900 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 4DC7C668B4145392

FASTA37342,072
        10         20         30         40         50         60 
MTEVLWPAVP NGTDAAFLAG PGSSWGNSTV ASTAAVSSSF KCALTKTGFQ FYYLPAVYIL 

        70         80         90        100        110        120 
VFIIGFLGNS VAIWMFVFHM KPWSGISVYM FNLALADFLY VLTLPALIFY YFNKTDWIFG 

       130        140        150        160        170        180 
DAMCKLQRFI FHVNLYGSIL FLTCISAHRY SGVVYPLKSL GRLKKKNAIC ISVLVWLIVV 

       190        200        210        220        230        240 
VAISPILFYS GTGVRKNKTI TCYDTTSDEY LRSYFIYSMC TTVAMFCVPL VLILGCYGLI 

       250        260        270        280        290        300 
VRALIYKDLD NSPLRRKSIY LVIIVLTVFA VSYIPFHVMK TMNLRARLDF QTPAMCAFND 

       310        320        330        340        350        360 
RVYATYQVTR GLASLNSCVD PILYFLAGDT FRRRLSRATR KASRRSEANL QSKSEDMTLN 

       370 
ILPEFKQNGD TSL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a human cDNA encoding endothelial P2Y1 purinoceptor."
Leon C., Vial C., Cazenave J.-P., Gachet C.
Gene 171:295-297(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Cloning and chromosomal localization of the human P2Y1 purinoceptor."
Ayyanathan K., Tania W., Harbansjit S., Raghbir A.S., Barnard E.A., Kunapuli S.P.
Biochem. Biophys. Res. Commun. 218:783-788(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning and tissue distribution of the human P2Y1 receptor."
Janssens R., Communi D., Pirotton S., Samson M., Parmentier M., Boeynaems J.-M.
Biochem. Biophys. Res. Commun. 221:588-593(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]Leon C., Vial C., Weber J., Cazenave J.-P., Gacher C.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Blood.
[5]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[7]"Molecular basis for ADP-induced platelet activation. II. The P2Y1 receptor mediates ADP-induced intracellular calcium mobilization and shape change in platelets."
Jin J., Daniel J.L., Kunapuli S.P.
J. Biol. Chem. 273:2030-2034(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 95-373, FUNCTION, INHIBITION.
Tissue: Platelet.
[8]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49205 mRNA. Translation: CAA89066.1.
U42030 mRNA. Translation: AAA97873.1.
U42029 mRNA. Translation: AAA97872.1.
S81950 Genomic DNA. Translation: AAB47091.1.
AJ006945 Genomic DNA. Translation: CAA07339.1.
AY136752 mRNA. Translation: AAN01278.1.
BC074784 mRNA. Translation: AAH74784.1.
BC074785 mRNA. Translation: AAH74785.1.
AF018284 mRNA. Translation: AAB94556.1.
PIRJC4737.
RefSeqNP_002554.1. NM_002563.3.
UniGeneHs.654526.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y36model-A1-373[»]
ProteinModelPortalP47900.
SMRP47900. Positions 23-342.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111067. 2 interactions.
IntActP47900. 1 interaction.
MINTMINT-1796592.
STRING9606.ENSP00000304767.

Chemistry

BindingDBP47900.
ChEMBLCHEMBL4315.
GuidetoPHARMACOLOGY323.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP47900.

Polymorphism databases

DMDM1352692.

Proteomic databases

PaxDbP47900.
PRIDEP47900.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000305097; ENSP00000304767; ENSG00000169860.
GeneID5028.
KEGGhsa:5028.
UCSCuc003ezq.3. human.

Organism-specific databases

CTD5028.
GeneCardsGC03P152552.
HGNCHGNC:8539. P2RY1.
HPAHPA015577.
HPA017674.
MIM601167. gene.
neXtProtNX_P47900.
PharmGKBPA32868.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG146579.
HOGENOMHOG000231307.
HOVERGENHBG101120.
InParanoidP47900.
KOK04270.
OMAKKNAVYI.
OrthoDBEOG7GFB51.
PhylomeDBP47900.
TreeFamTF350009.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.

Gene expression databases

BgeeP47900.
CleanExHS_P2RY1.
GenevestigatorP47900.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000142. P2Y1_rcpt.
[Graphical view]
PANTHERPTHR24231:SF2. PTHR24231:SF2. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00595. P2Y1PRNOCPTR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiP2RY1.
GenomeRNAi5028.
NextBio19362.
PROP47900.
SOURCESearch...

Entry information

Entry nameP2RY1_HUMAN
AccessionPrimary (citable) accession number: P47900
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries