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Protein

Insulin-like growth factor-binding protein 3

Gene

Igfbp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

GO - Molecular functioni

  • fibronectin binding Source: MGI
  • insulin-like growth factor binding Source: MGI
  • insulin-like growth factor I binding Source: MGI
  • protein tyrosine phosphatase activator activity Source: UniProtKB

GO - Biological processi

  • negative regulation of cell proliferation Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • negative regulation of smooth muscle cell migration Source: MGI
  • negative regulation of smooth muscle cell proliferation Source: MGI
  • osteoblast differentiation Source: BHF-UCL
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of catalytic activity Source: MGI
  • positive regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of myoblast differentiation Source: UniProtKB
  • protein phosphorylation Source: MGI
  • regulation of cell growth Source: MGI
  • regulation of glucose metabolic process Source: MGI
  • regulation of growth Source: MGI
  • type B pancreatic cell proliferation Source: MGI
Complete GO annotation...

Keywords - Ligandi

Growth factor binding

Enzyme and pathway databases

ReactomeiR-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiI31.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 3
Short name:
IBP-3
Short name:
IGF-binding protein 3
Short name:
IGFBP-3
Gene namesi
Name:Igfbp3
Synonyms:Igfbp-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96438. Igfbp3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 292265Insulin-like growth factor-binding protein 3PRO_0000014379Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Modified residuei149 – 1491PhosphoserineBy similarity
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence analysis
Modified residuei202 – 2021PhosphoserineBy similarity
Disulfide bondi214 ↔ 241PROSITE-ProRule annotation
Disulfide bondi252 ↔ 263PROSITE-ProRule annotation
Disulfide bondi265 ↔ 286PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP47878.
PRIDEiP47878.

PTM databases

iPTMnetiP47878.
PhosphoSiteiP47878.

Expressioni

Gene expression databases

CleanExiMM_IGFBP3.
GenevisibleiP47878. MM.

Interactioni

Subunit structurei

Interacts with XLKD1. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219 (By similarity).By similarity

GO - Molecular functioni

  • fibronectin binding Source: MGI
  • insulin-like growth factor binding Source: MGI
  • insulin-like growth factor I binding Source: MGI

Protein-protein interaction databases

IntActiP47878. 1 interaction.
STRINGi10090.ENSMUSP00000020702.

Structurei

3D structure databases

ProteinModelPortaliP47878.
SMRiP47878. Positions 76-118, 212-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 11984IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini211 – 28676Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHZU. Eukaryota.
ENOG4111MF3. LUCA.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP47878.
KOiK10138.
OMAiRALEQCK.
OrthoDBiEOG74N5HG.
PhylomeDBiP47878.
TreeFamiTF331211.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALSQ
60 70 80 90 100
CAPPPTAPAC TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP
110 120 130 140 150
AEQYPLRALL NGRGFCANAS AAGSLSTYLP SQPAPGNISE SEEEHNAGSV
160 170 180 190 200
ESQVVPSTHR VTDSKFHPLH AKMDVIKKGH ARDSQRYKVD YESQSTDTQN
210 220 230 240 250
FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK
260 270 280 290
QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ
Length:292
Mass (Da):31,687
Last modified:October 16, 2013 - v2
Checksum:i317F830AA843B73D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 283RAG → ELA in CAA57271 (PubMed:7529732).Curated
Sequence conflicti34 – 341Missing in CAA57271 (PubMed:7529732).Curated
Sequence conflicti48 – 481L → V in CAA57271 (PubMed:7529732).Curated
Sequence conflicti251 – 2511Q → R in CAA57271 (PubMed:7529732).Curated
Sequence conflicti260 – 2612RG → QS in CAA57271 (PubMed:7529732).Curated
Sequence conflicti272 – 2721P → R in CAA57271 (PubMed:7529732).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81581 mRNA. Translation: CAA57271.1.
AL607124 Genomic DNA. No translation available.
BC058261 mRNA. Translation: AAH58261.1.
CCDSiCCDS24428.1.
PIRiI48602.
RefSeqiNP_032369.2. NM_008343.2.
UniGeneiMm.29254.

Genome annotation databases

EnsembliENSMUST00000020702; ENSMUSP00000020702; ENSMUSG00000020427.
ENSMUST00000135887; ENSMUSP00000131670; ENSMUSG00000020427.
GeneIDi16009.
KEGGimmu:16009.
UCSCiuc007hzi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81581 mRNA. Translation: CAA57271.1.
AL607124 Genomic DNA. No translation available.
BC058261 mRNA. Translation: AAH58261.1.
CCDSiCCDS24428.1.
PIRiI48602.
RefSeqiNP_032369.2. NM_008343.2.
UniGeneiMm.29254.

3D structure databases

ProteinModelPortaliP47878.
SMRiP47878. Positions 76-118, 212-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP47878. 1 interaction.
STRINGi10090.ENSMUSP00000020702.

Protein family/group databases

MEROPSiI31.952.

PTM databases

iPTMnetiP47878.
PhosphoSiteiP47878.

Proteomic databases

PaxDbiP47878.
PRIDEiP47878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020702; ENSMUSP00000020702; ENSMUSG00000020427.
ENSMUST00000135887; ENSMUSP00000131670; ENSMUSG00000020427.
GeneIDi16009.
KEGGimmu:16009.
UCSCiuc007hzi.2. mouse.

Organism-specific databases

CTDi3486.
MGIiMGI:96438. Igfbp3.

Phylogenomic databases

eggNOGiENOG410IHZU. Eukaryota.
ENOG4111MF3. LUCA.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP47878.
KOiK10138.
OMAiRALEQCK.
OrthoDBiEOG74N5HG.
PhylomeDBiP47878.
TreeFamiTF331211.

Enzyme and pathway databases

ReactomeiR-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi288818.
PROiP47878.
SOURCEiSearch...

Gene expression databases

CleanExiMM_IGFBP3.
GenevisibleiP47878. MM.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR012211. IGFBP-3.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF3. PTHR11551:SF3. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01979. IGFBPFAMILY3.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and mRNA expression of the six mouse insulin-like growth factor binding proteins."
    Schuller A.G.P., Groffen C., van Neck J.W., Zwarthoff E.C., Drop S.L.S.
    Mol. Cell. Endocrinol. 104:57-66(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiIBP3_MOUSE
AccessioniPrimary (citable) accession number: P47878
Secondary accession number(s): Q6PE62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 16, 2013
Last modified: May 11, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.