P47877 (IBP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 2
Short name=IBP-2
Short name=IGF-binding protein 2
Short name=IGFBP-2
Short name=mIGFBP-2

Gene names

Name:	Igfbp2
Synonyms:	Igfbp-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits IGF-mediated growth and developmental rates By similarity. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subunit structure	Binds IGF2 more than IGF1.
Subcellular location	Secreted.
Tissue specificity	Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. Ref.2
Developmental stage	Expressed embryos at day 11.5. Also expressed in fetal livers with expression increasing dramatically after birth. Expression decreases only slightly after postnatal day 3 and remains abundant thereafter. Ref.2
Domain	The C-terminus is required for IGF-binding and growth inhibition By similarity.
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Biological process	Growth regulation
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from electronic annotation. Source: Compara cellular response to hormone stimulus Inferred from electronic annotation. Source: Compara female pregnancy Inferred from electronic annotation. Source: Compara positive regulation of activated T cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell growth Inferred from electronic annotation. Source: InterPro regulation of insulin-like growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB response to drug Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to lithium ion Inferred from electronic annotation. Source: Compara response to mechanical stimulus Inferred from electronic annotation. Source: Compara response to nutrient Inferred from electronic annotation. Source: Compara response to retinoic acid Inferred from electronic annotation. Source: Compara response to stress Inferred from electronic annotation. Source: Compara signal transduction Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara cytoplasmic vesicle Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 7510770PubMed 7679139. Source: MGI
Molecular_function	insulin-like growth factor I binding Inferred from sequence or structural similarity. Source: UniProtKB insulin-like growth factor II binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	By similarity
	Chain	35 – 305	271	Insulin-like growth factor-binding protein 2		PRO_0000014371
Regions
	Domain	36 – 118	83	IGFBP N-terminal
	Domain	204 – 286	83	Thyroglobulin type-1
	Motif	281 – 283	3	Cell attachment site
Amino acid modifications
	Disulfide bond	207 ↔ 241		By similarity
	Disulfide bond	252 ↔ 263		By similarity
	Disulfide bond	265 ↔ 286		By similarity
Experimental info
	Sequence conflict	47	1	R → A in CAA57270. Ref.2
	Sequence conflict	133	1	D → H in AAB60709. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47877 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 179FE6A304E93A16	FASTA	305	32,847
	10 20 30 40 50 60 MLPRLGGPAL PLLLPSLLLL LLLGAGGCGP GVRAEVLFRC PPCTPERLAA CGPPPDAPCA 70 80 90 100 110 120 ELVREPGCGC CSVCARQEGE ACGVYIPRCA QTLRCYPNPG SELPLKALVT GAGTCEKRRV 130 140 150 160 170 180 GTTPQQVADS DDDHSEGGLV ENHVDGTMNM LGGGSSAGRK PLKSGMKELA VFREKVNEQH 190 200 210 220 230 240 RQMGKGAKHL SLEEPKKLRP PPARTPCQQE LDQVLERIST MRLPDDRGPL EHLYSLHIPN 250 260 270 280 290 300 CDKHGRYNLK QCKMSLNGQR GECWCVNPNT GKPIQGAPTI RGDPECHLFY NEQQETGGAH AQSVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the gene encoding murine insulin-like growth factor-binding protein-2, mIGFBP-2." Landwehr J., Kaupmann K., Heinrich G., Schwander J. Gene 124:281-286(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Fetal liver.
[2]	"cDNA cloning and mRNA expression of the six mouse insulin-like growth factor binding proteins." Schuller A.G.P., Groffen C., van Neck J.W., Zwarthoff E.C., Drop S.L.S. Mol. Cell. Endocrinol. 104:57-66(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Liver.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N-3. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L05439 , L05436, L05437, L05438 Genomic DNA. Translation: AAB60709.1. X81580 mRNA. Translation: CAA57270.1. CH466548 Genomic DNA. Translation: EDL00293.1. BC012724 mRNA. Translation: AAH12724.1. BC054473 mRNA. Translation: AAH54473.1.
IPI	IPI00313327.
PIR	I48601. JN0508.
RefSeq	NP_032368.2. NM_008342.3.
UniGene	Mm.141936.
3D structure databases
ProteinModelPortal	P47877.
SMR	P47877. Positions 200-305.
ModBase	Search...
Protein family/group databases
MEROPS	I31.953.
Proteomic databases
PaxDb	P47877.
PRIDE	P47877.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047328; ENSMUSP00000046610; ENSMUSG00000039323.
GeneID	16008.
KEGG	mmu:16008.
UCSC	uc007bkw.1. mouse.
Organism-specific databases
CTD	3485.
MGI	MGI:96437. Igfbp2.
Phylogenomic databases
eggNOG	NOG47500.
GeneTree	ENSGT00550000074457.
HOGENOM	HOG000253012.
HOVERGEN	HBG002631.
InParanoid	Q91VK7.
OMA	GAGTCEK.
OrthoDB	EOG4TTGJD.
Gene expression databases
ArrayExpress	P47877.
Bgee	P47877.
CleanEx	MM_IGFBP2.
Genevestigator	P47877.
GermOnline	ENSMUSG00000039323. Mus musculus.
Family and domain databases
Gene3D	4.10.800.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt. IPR012210. IGFBP-2. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
PANTHER	PTHR11551. PTHR11551. 1 hit. PTHR11551:SF5. PTHR11551:SF5. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01978. IGFBPFAMILY2.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	IGFBP2. mouse.
NextBio	288814.
SOURCE	Search...

Entry information

Entry name

IBP2_MOUSE

Accession

Primary (citable) accession number: P47877
Secondary accession number(s): Q61722, Q91VK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program