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P47866

- CRFR2_RAT

UniProt

P47866 - CRFR2_RAT

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Protein
Corticotropin-releasing factor receptor 2
Gene
Crhr2, Crf2r
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.1 Publication

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: RGD
  2. corticotropin-releasing hormone receptor activity Source: RGD
  3. peptide hormone binding Source: RGD

GO - Biological processi

  1. actin filament organization Source: RGD
  2. catecholamine biosynthetic process Source: RGD
  3. cellular response to glucocorticoid stimulus Source: RGD
  4. cerebellum development Source: RGD
  5. epithelial cell differentiation Source: RGD
  6. feeding behavior Source: RGD
  7. gastric motility Source: RGD
  8. hormone-mediated signaling pathway Source: GOC
  9. hypothalamus development Source: RGD
  10. long-term synaptic potentiation Source: RGD
  11. negative regulation of cAMP biosynthetic process Source: RGD
  12. negative regulation of calcium ion import Source: RGD
  13. negative regulation of defecation Source: RGD
  14. negative regulation of epinephrine secretion Source: RGD
  15. negative regulation of feeding behavior Source: RGD
  16. negative regulation of follicle-stimulating hormone secretion Source: RGD
  17. negative regulation of gene expression Source: RGD
  18. negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: RGD
  19. negative regulation of luteinizing hormone secretion Source: RGD
  20. negative regulation of norepinephrine secretion Source: RGD
  21. positive regulation of NF-kappaB transcription factor activity Source: RGD
  22. positive regulation of blood pressure Source: RGD
  23. positive regulation of cAMP biosynthetic process Source: RGD
  24. positive regulation of cAMP metabolic process Source: RGD
  25. positive regulation of gene expression Source: RGD
  26. positive regulation of heart rate Source: RGD
  27. positive regulation of interleukin-6 production Source: RGD
  28. positive regulation of serotonin secretion Source: RGD
  29. positive regulation of stress-activated MAPK cascade Source: RGD
  30. protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
  31. regulation of ERK1 and ERK2 cascade Source: RGD
  32. sensory perception of pain Source: RGD
  33. skeletal muscle tissue growth Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Corticotropin-releasing factor receptor 2
Short name:
CRF-R-2
Short name:
CRF-R2
Short name:
CRFR-2
Alternative name(s):
Corticotropin-releasing hormone receptor 2
Short name:
CRH-R-2
Short name:
CRH-R2
Gene namesi
Name:Crhr2
Synonyms:Crf2r
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi70547. Crhr2.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 108108Extracellular By similarity
Add
BLAST
Transmembranei109 – 13931Helical; Name=1; By similarity
Add
BLAST
Topological domaini140 – 1467Cytoplasmic By similarity
Transmembranei147 – 17125Helical; Name=2; By similarity
Add
BLAST
Topological domaini172 – 18514Extracellular By similarity
Add
BLAST
Transmembranei186 – 21429Helical; Name=3; By similarity
Add
BLAST
Topological domaini215 – 2217Cytoplasmic By similarity
Transmembranei222 – 24928Helical; Name=4; By similarity
Add
BLAST
Topological domaini250 – 26516Extracellular By similarity
Add
BLAST
Transmembranei266 – 29126Helical; Name=5; By similarity
Add
BLAST
Topological domaini292 – 30211Cytoplasmic By similarity
Add
BLAST
Transmembranei303 – 32725Helical; Name=6; By similarity
Add
BLAST
Topological domaini328 – 3347Extracellular By similarity
Transmembranei335 – 36430Helical; Name=7; By similarity
Add
BLAST
Topological domaini365 – 41147Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: RGD
  2. axon Source: RGD
  3. axon terminus Source: RGD
  4. cell body fiber Source: RGD
  5. dendrite Source: RGD
  6. integral component of membrane Source: UniProtKB-KW
  7. neuronal cell body Source: RGD
  8. perikaryon Source: RGD
  9. plasma membrane Source: RGD
  10. rough endoplasmic reticulum Source: RGD
  11. varicosity Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi13 – 131N → A, F or I: Allows cleavage of signal peptide. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Corticotropin-releasing factor receptor 2
PRO_0000012822Add
BLAST
Signal peptidei1 – 1919Not cleaved
Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi13 – 131N-linked (GlcNAc...)1 Publication
Disulfide bondi14 ↔ 50 By similarity
Disulfide bondi40 ↔ 83 By similarity
Glycosylationi41 – 411N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi64 ↔ 98 By similarity
Glycosylationi74 – 741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi86 – 861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi94 – 941N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi184 ↔ 254 By similarity

Post-translational modificationi

A N-glycosylation site within the signal peptide impedes its proper cleavage and function.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP47866.

PTM databases

PhosphoSiteiP47866.

Expressioni

Tissue specificityi

Predominantly expressed in limbic regions of the brain such as the lateral septum, the entorhinal cortex, the hypothalamic ventromedial nucleus and several amygdaloid nuclei. Also detectable in lung, kidney and heart.1 Publication

Gene expression databases

GenevestigatoriP47866.

Interactioni

Subunit structurei

Monomer. Interacts (via N-terminal extracellular domain) with CRF, UCN, UCN2 and UCN3 By similarity.

Protein-protein interaction databases

BioGridi249194. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliP47866.

Family & Domainsi

Domaini

The transmembrane domain is composed of seven transmembrane helices that are arranged in V-shape. Transmembrane helix 7 assumes a sharply kinked structure By similarity.
The uncleaved pseudo signal peptide prevents receptor's oligomerization and coupling to G(i) subunits. It is also responsible for the rather low receptor localization at the plasma membrane By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295825.
GeneTreeiENSGT00750000117453.
HOGENOMiHOG000230719.
HOVERGENiHBG106921.
KOiK04579.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR003053. GPCR_2_CRF2_rcpt.
IPR003051. GPCR_2_CRF_rcpt.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01279. CRFRECEPTOR.
PR01281. CRFRECEPTOR2.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform CRF2-alpha (identifier: P47866-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDAALLLSLL EANCSLALAE ELLLDGWGEP PDPEGPYSYC NTTLDQIGTC    50
WPQSAPGALV ERPCPEYFNG IKYNTTRNAY RECLENGTWA SRVNYSHCEP 100
ILDDKQRKYD LHYRIALIIN YLGHCVSVVA LVAAFLLFLV LRSIRCLRNV 150
IHWNLITTFI LRNITWFLLQ LIDHEVHEGN EVWCRCVTTI FNYFVVTNFF 200
WMFVEGCYLH TAIVMTYSTE HLRKWLFLFI GWCIPCPIIV AWAVGKLYYE 250
NEQCWFGKEP GDLVDYIYQG PIILVLLINF VFLFNIVRIL MTKLRASTTS 300
ETIQYRKAVK ATLVLLPLLG ITYMLFFVNP GEDDLSQIVF IYFNSFLQSF 350
QGFFVSVFYC FFNGEVRSAL RKRWHRWQDH HALRVPVARA MSIPTSPTRI 400
SFHSIKQTAA V 411

Note: Major isoform.

Length:411
Mass (Da):47,693
Last modified:January 22, 2014 - v2
Checksum:iF0BA7795F8C37AFE
GO
Isoform CRF2-beta (identifier: P47866-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MDAALLLSLLEANCSLALAEELLLDGWGEPPDPE → MGHPGSLPSA...CHRTTTRNFS

Show »
Length:431
Mass (Da):50,187
Checksum:i593B8DE5E58B057D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3434MDAAL…PPDPE → MGHPGSLPSAQLLLCLYSLL PLLQVAQPGRPLQDQPLWTL LEQYCHRTTTRNFS in isoform CRF2-beta.
VSP_002001Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931V → I in AAC52159. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16253 mRNA. Translation: AAC52159.1.
AABR06031050 Genomic DNA. No translation available.
CH474011 Genomic DNA. Translation: EDL88099.1.
PIRiA55610.
RefSeqiNP_073205.1. NM_022714.1.
UniGeneiRn.10023.

Genome annotation databases

EnsembliENSRNOT00000033672; ENSRNOP00000035712; ENSRNOG00000011145. [P47866-1]
GeneIDi64680.
KEGGirno:64680.
UCSCiRGD:70547. rat. [P47866-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16253 mRNA. Translation: AAC52159.1 .
AABR06031050 Genomic DNA. No translation available.
CH474011 Genomic DNA. Translation: EDL88099.1 .
PIRi A55610.
RefSeqi NP_073205.1. NM_022714.1.
UniGenei Rn.10023.

3D structure databases

ProteinModelPortali P47866.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249194. 7 interactions.

Chemistry

BindingDBi P47866.
ChEMBLi CHEMBL2111360.
GuidetoPHARMACOLOGYi 213.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P47866.

Proteomic databases

PRIDEi P47866.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000033672 ; ENSRNOP00000035712 ; ENSRNOG00000011145 . [P47866-1 ]
GeneIDi 64680.
KEGGi rno:64680.
UCSCi RGD:70547. rat. [P47866-1 ]

Organism-specific databases

CTDi 1395.
RGDi 70547. Crhr2.

Phylogenomic databases

eggNOGi NOG295825.
GeneTreei ENSGT00750000117453.
HOGENOMi HOG000230719.
HOVERGENi HBG106921.
KOi K04579.

Miscellaneous databases

NextBioi 35584281.
PROi P47866.

Gene expression databases

Genevestigatori P47866.

Family and domain databases

InterProi IPR017981. GPCR_2-like.
IPR003053. GPCR_2_CRF2_rcpt.
IPR003051. GPCR_2_CRF_rcpt.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view ]
Pfami PF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view ]
PRINTSi PR01279. CRFRECEPTOR.
PR01281. CRFRECEPTOR2.
PR00249. GPCRSECRETIN.
SMARTi SM00008. HormR. 1 hit.
[Graphical view ]
PROSITEi PS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a functionally distinct corticotropin-releasing factor receptor subtype from rat brain."
    Lovenberg T.W., Liaw C.W., Grigoriadis D.E., Clevenger W., Chalmers D.T., de Souza E.B., Oltersdorf T.
    Proc. Natl. Acad. Sci. U.S.A. 92:836-840(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Hypothalamus and Lung.
  2. Erratum
    Lovenberg T.W., Liaw C.W., Grigoriadis D.E., Clevenger W., Chalmers D.T., de Souza E.B., Oltersdorf T.
    Proc. Natl. Acad. Sci. U.S.A. 92:5759-5759(1995)
  3. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  5. "The corticotropin-releasing factor receptor type 2a contains an N-terminal pseudo signal peptide."
    Rutz C., Renner A., Alken M., Schulz K., Beyermann M., Wiesner B., Rosenthal W., Schulein R.
    J. Biol. Chem. 281:24910-24921(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-13, MUTAGENESIS OF ASN-13, NON-CLEAVABLE SIGNAL SEQUENCE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCRFR2_RAT
AccessioniPrimary (citable) accession number: P47866
Secondary accession number(s): G3V948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 22, 2014
Last modified: September 3, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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