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P47866

- CRFR2_RAT

UniProt

P47866 - CRFR2_RAT

Protein

Corticotropin-releasing factor receptor 2

Gene

Crhr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (22 Jan 2014)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.1 Publication

    GO - Molecular functioni

    1. corticotropin-releasing hormone receptor activity Source: RGD
    2. G-protein coupled receptor activity Source: RGD
    3. peptide hormone binding Source: RGD

    GO - Biological processi

    1. actin filament organization Source: RGD
    2. catecholamine biosynthetic process Source: RGD
    3. cellular response to glucocorticoid stimulus Source: RGD
    4. cerebellum development Source: RGD
    5. epithelial cell differentiation Source: RGD
    6. feeding behavior Source: RGD
    7. gastric motility Source: RGD
    8. hormone-mediated signaling pathway Source: GOC
    9. hypothalamus development Source: RGD
    10. long-term synaptic potentiation Source: RGD
    11. negative regulation of calcium ion import Source: RGD
    12. negative regulation of cAMP biosynthetic process Source: RGD
    13. negative regulation of defecation Source: RGD
    14. negative regulation of epinephrine secretion Source: RGD
    15. negative regulation of feeding behavior Source: RGD
    16. negative regulation of follicle-stimulating hormone secretion Source: RGD
    17. negative regulation of gene expression Source: RGD
    18. negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: RGD
    19. negative regulation of luteinizing hormone secretion Source: RGD
    20. negative regulation of norepinephrine secretion Source: RGD
    21. positive regulation of blood pressure Source: RGD
    22. positive regulation of cAMP biosynthetic process Source: RGD
    23. positive regulation of cAMP metabolic process Source: RGD
    24. positive regulation of gene expression Source: RGD
    25. positive regulation of heart rate Source: RGD
    26. positive regulation of interleukin-6 production Source: RGD
    27. positive regulation of NF-kappaB transcription factor activity Source: RGD
    28. positive regulation of serotonin secretion Source: RGD
    29. positive regulation of stress-activated MAPK cascade Source: RGD
    30. protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
    31. regulation of ERK1 and ERK2 cascade Source: RGD
    32. sensory perception of pain Source: RGD
    33. skeletal muscle tissue growth Source: RGD

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Corticotropin-releasing factor receptor 2
    Short name:
    CRF-R-2
    Short name:
    CRF-R2
    Short name:
    CRFR-2
    Alternative name(s):
    Corticotropin-releasing hormone receptor 2
    Short name:
    CRH-R-2
    Short name:
    CRH-R2
    Gene namesi
    Name:Crhr2
    Synonyms:Crf2r
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi70547. Crhr2.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. axon Source: RGD
    2. axon terminus Source: RGD
    3. cell body fiber Source: RGD
    4. dendrite Source: RGD
    5. Golgi apparatus Source: RGD
    6. integral component of membrane Source: UniProtKB-KW
    7. neuronal cell body Source: RGD
    8. perikaryon Source: RGD
    9. plasma membrane Source: RGD
    10. rough endoplasmic reticulum Source: RGD
    11. varicosity Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi13 – 131N → A, F or I: Allows cleavage of signal peptide. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411Corticotropin-releasing factor receptor 2PRO_0000012822Add
    BLAST
    Signal peptidei1 – 1919Not cleavedAdd
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi13 – 131N-linked (GlcNAc...)1 Publication
    Disulfide bondi14 ↔ 50By similarity
    Disulfide bondi40 ↔ 83By similarity
    Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi64 ↔ 98By similarity
    Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi184 ↔ 254By similarity

    Post-translational modificationi

    A N-glycosylation site within the signal peptide impedes its proper cleavage and function.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP47866.

    PTM databases

    PhosphoSiteiP47866.

    Expressioni

    Tissue specificityi

    Predominantly expressed in limbic regions of the brain such as the lateral septum, the entorhinal cortex, the hypothalamic ventromedial nucleus and several amygdaloid nuclei. Also detectable in lung, kidney and heart.1 Publication

    Gene expression databases

    GenevestigatoriP47866.

    Interactioni

    Subunit structurei

    Monomer. Interacts (via N-terminal extracellular domain) with CRF, UCN, UCN2 and UCN3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi249194. 7 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP47866.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 108108ExtracellularBy similarityAdd
    BLAST
    Topological domaini140 – 1467CytoplasmicBy similarity
    Topological domaini172 – 18514ExtracellularBy similarityAdd
    BLAST
    Topological domaini215 – 2217CytoplasmicBy similarity
    Topological domaini250 – 26516ExtracellularBy similarityAdd
    BLAST
    Topological domaini292 – 30211CytoplasmicBy similarityAdd
    BLAST
    Topological domaini328 – 3347ExtracellularBy similarity
    Topological domaini365 – 41147CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei109 – 13931Helical; Name=1By similarityAdd
    BLAST
    Transmembranei147 – 17125Helical; Name=2By similarityAdd
    BLAST
    Transmembranei186 – 21429Helical; Name=3By similarityAdd
    BLAST
    Transmembranei222 – 24928Helical; Name=4By similarityAdd
    BLAST
    Transmembranei266 – 29126Helical; Name=5By similarityAdd
    BLAST
    Transmembranei303 – 32725Helical; Name=6By similarityAdd
    BLAST
    Transmembranei335 – 36430Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Domaini

    The transmembrane domain is composed of seven transmembrane helices that are arranged in V-shape. Transmembrane helix 7 assumes a sharply kinked structure By similarity.By similarity
    The uncleaved pseudo signal peptide prevents receptor's oligomerization and coupling to G(i) subunits. It is also responsible for the rather low receptor localization at the plasma membrane By similarity.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295825.
    GeneTreeiENSGT00750000117453.
    HOGENOMiHOG000230719.
    HOVERGENiHBG106921.
    KOiK04579.

    Family and domain databases

    InterProiIPR017981. GPCR_2-like.
    IPR003053. GPCR_2_CRF2_rcpt.
    IPR003051. GPCR_2_CRF_rcpt.
    IPR001879. GPCR_2_extracellular_dom.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view]
    PfamiPF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view]
    PRINTSiPR01279. CRFRECEPTOR.
    PR01281. CRFRECEPTOR2.
    PR00249. GPCRSECRETIN.
    SMARTiSM00008. HormR. 1 hit.
    [Graphical view]
    PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform CRF2-alpha (identifier: P47866-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDAALLLSLL EANCSLALAE ELLLDGWGEP PDPEGPYSYC NTTLDQIGTC    50
    WPQSAPGALV ERPCPEYFNG IKYNTTRNAY RECLENGTWA SRVNYSHCEP 100
    ILDDKQRKYD LHYRIALIIN YLGHCVSVVA LVAAFLLFLV LRSIRCLRNV 150
    IHWNLITTFI LRNITWFLLQ LIDHEVHEGN EVWCRCVTTI FNYFVVTNFF 200
    WMFVEGCYLH TAIVMTYSTE HLRKWLFLFI GWCIPCPIIV AWAVGKLYYE 250
    NEQCWFGKEP GDLVDYIYQG PIILVLLINF VFLFNIVRIL MTKLRASTTS 300
    ETIQYRKAVK ATLVLLPLLG ITYMLFFVNP GEDDLSQIVF IYFNSFLQSF 350
    QGFFVSVFYC FFNGEVRSAL RKRWHRWQDH HALRVPVARA MSIPTSPTRI 400
    SFHSIKQTAA V 411

    Note: Major isoform.

    Length:411
    Mass (Da):47,693
    Last modified:January 22, 2014 - v2
    Checksum:iF0BA7795F8C37AFE
    GO
    Isoform CRF2-beta (identifier: P47866-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-34: MDAALLLSLLEANCSLALAEELLLDGWGEPPDPE → MGHPGSLPSA...CHRTTTRNFS

    Show »
    Length:431
    Mass (Da):50,187
    Checksum:i593B8DE5E58B057D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 931V → I in AAC52159. (PubMed:7846062)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 3434MDAAL…PPDPE → MGHPGSLPSAQLLLCLYSLL PLLQVAQPGRPLQDQPLWTL LEQYCHRTTTRNFS in isoform CRF2-beta. CuratedVSP_002001Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16253 mRNA. Translation: AAC52159.1.
    AABR06031050 Genomic DNA. No translation available.
    CH474011 Genomic DNA. Translation: EDL88099.1.
    PIRiA55610.
    RefSeqiNP_073205.1. NM_022714.1.
    UniGeneiRn.10023.

    Genome annotation databases

    EnsembliENSRNOT00000033672; ENSRNOP00000035712; ENSRNOG00000011145. [P47866-1]
    GeneIDi64680.
    KEGGirno:64680.
    UCSCiRGD:70547. rat. [P47866-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16253 mRNA. Translation: AAC52159.1 .
    AABR06031050 Genomic DNA. No translation available.
    CH474011 Genomic DNA. Translation: EDL88099.1 .
    PIRi A55610.
    RefSeqi NP_073205.1. NM_022714.1.
    UniGenei Rn.10023.

    3D structure databases

    ProteinModelPortali P47866.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249194. 7 interactions.

    Chemistry

    BindingDBi P47866.
    ChEMBLi CHEMBL2111360.
    GuidetoPHARMACOLOGYi 213.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P47866.

    Proteomic databases

    PRIDEi P47866.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000033672 ; ENSRNOP00000035712 ; ENSRNOG00000011145 . [P47866-1 ]
    GeneIDi 64680.
    KEGGi rno:64680.
    UCSCi RGD:70547. rat. [P47866-1 ]

    Organism-specific databases

    CTDi 1395.
    RGDi 70547. Crhr2.

    Phylogenomic databases

    eggNOGi NOG295825.
    GeneTreei ENSGT00750000117453.
    HOGENOMi HOG000230719.
    HOVERGENi HBG106921.
    KOi K04579.

    Miscellaneous databases

    NextBioi 35584281.
    PROi P47866.

    Gene expression databases

    Genevestigatori P47866.

    Family and domain databases

    InterProi IPR017981. GPCR_2-like.
    IPR003053. GPCR_2_CRF2_rcpt.
    IPR003051. GPCR_2_CRF_rcpt.
    IPR001879. GPCR_2_extracellular_dom.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view ]
    Pfami PF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view ]
    PRINTSi PR01279. CRFRECEPTOR.
    PR01281. CRFRECEPTOR2.
    PR00249. GPCRSECRETIN.
    SMARTi SM00008. HormR. 1 hit.
    [Graphical view ]
    PROSITEi PS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a functionally distinct corticotropin-releasing factor receptor subtype from rat brain."
      Lovenberg T.W., Liaw C.W., Grigoriadis D.E., Clevenger W., Chalmers D.T., de Souza E.B., Oltersdorf T.
      Proc. Natl. Acad. Sci. U.S.A. 92:836-840(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Hypothalamus and Lung.
    2. Erratum
      Lovenberg T.W., Liaw C.W., Grigoriadis D.E., Clevenger W., Chalmers D.T., de Souza E.B., Oltersdorf T.
      Proc. Natl. Acad. Sci. U.S.A. 92:5759-5759(1995)
    3. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
      , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
      Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    5. "The corticotropin-releasing factor receptor type 2a contains an N-terminal pseudo signal peptide."
      Rutz C., Renner A., Alken M., Schulz K., Beyermann M., Wiesner B., Rosenthal W., Schulein R.
      J. Biol. Chem. 281:24910-24921(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-13, MUTAGENESIS OF ASN-13, NON-CLEAVABLE SIGNAL SEQUENCE, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCRFR2_RAT
    AccessioniPrimary (citable) accession number: P47866
    Secondary accession number(s): G3V948
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 22, 2014
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3