P47862 (AQP3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin-3
Short name=AQP-3

Alternative name(s):
31.4 kDa water channel protein
Aquaglyceroporin-3

Gene names

Name:

Aqp3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Ref.1
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Renal medulla and colon. Predominantly in the inner medulla.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]
Sequence caution	The sequence BAA04559.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	water channel activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Aquaporin-3		PRO_0000063945
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 49	21	Helical; Potential
	Topological domain	50 – 53	4	Extracellular Potential
	Transmembrane	54 – 74	21	Helical; Potential
	Topological domain	75 – 109	35	Cytoplasmic Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Topological domain	131 – 157	27	Extracellular Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Topological domain	179 – 188	10	Cytoplasmic Potential
	Transmembrane	189 – 209	21	Helical; Potential
	Topological domain	210 – 244	35	Extracellular Potential
	Transmembrane	245 – 265	21	Helical; Potential
	Topological domain	266 – 292	27	Cytoplasmic Potential
	Motif	83 – 85	3	NPA 1
	Motif	215 – 217	3	NPA 2
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	273	1	P → L in AAI27491. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47862 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 41D9D3119DBA55AD	FASTA	292	31,384
	10 20 30 40 50 60 MGRQKELMNR CGEMLHIRYR LLRQALAECL GTLILVMFGC GSVAQVVLSR GTHGGFLTIN 70 80 90 100 110 120 LAFGFAVTLA ILVAGQVSGA HLNPAVTFAM CFLAREPWIK LPIYTLAQTL GAFLGAGIVF 130 140 150 160 170 180 GLYYDAIWAF AGNELVVSGP NGTAGIFATY PSGHLDMVNG FFDQFIGTAA LIVCVLAIVD 190 200 210 220 230 240 PYNNPVPRGL EAFTVGLVVL VIGTSMGFNS GYAVNPARDF GPRLFTALAG WGSEVFTTGQ 250 260 270 280 290 NWWWVPIVSP LLGSIGGVFV YQLMIGCHLE QPPPSTEAEN VKLAHMKHKE QI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of AQP3, a water channel from the medullary collecting duct of rat kidney." Echevarria M., Windhager E.E., Tate S.S., Frindt G. Proc. Natl. Acad. Sci. U.S.A. 91:10997-11001(1994) [PubMed: 7526388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells." Ishibashi K., Sasaki S., Fushimi K., Uchida S., Kuwahara M., Saito H., Furukawa T., Nakajima K., Yamaguchi Y., Gojobori T., Marumo F. Proc. Natl. Acad. Sci. U.S.A. 91:6269-6273(1994) [PubMed: 7517548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-292. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L35108 mRNA. Translation: AAA53652.1. BC127490 mRNA. Translation: AAI27491.1. D17695 mRNA. Translation: BAA04559.1. Different initiation.
IPI	IPI00191131.
PIR	I59266.
RefSeq	NP_113891.1. NM_031703.1.
UniGene	Rn.11109.
3D structure databases
ProteinModelPortal	P47862.
ModBase	Search...
Protein-protein interaction databases
STRING	P47862.
Protein family/group databases
TCDB	1.A.8.9.1. major intrinsic protein (MIP) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000013803; ENSRNOP00000013803; ENSRNOG00000009797.
GeneID	65133.
KEGG	rno:65133.
NMPDR	fig|10116.3.peg.22978.
UCSC	NM_031703. rat.
Organism-specific databases
CTD	360.
RGD	68428. Aqp3.
Phylogenomic databases
GeneTree	ENSGT00510000046311.
HOVERGEN	HBG106057.
InParanoid	P47862.
OrthoDB	EOG4XD3RR.
PhylomeDB	P47862.
Gene expression databases
ArrayExpress	P47862.
Genevestigator	P47862.
GermOnline	ENSRNOG00000009797. Rattus norvegicus.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR023271. Aquaporin-like. IPR023275. Aquaporin_3. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
KO	K09876.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR02015. AQUAPORIN3. PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613939.

Entry information

Entry name

AQP3_RAT

Accession

Primary (citable) accession number: P47862
Secondary accession number(s): A0JPL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program