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Protein

Aquaporin-3

Gene

Aqp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi1.A.8.9.1. the major intrinsic protein (mip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-3
Short name:
AQP-3
Alternative name(s):
31.4 kDa water channel protein
Aquaglyceroporin-3
Gene namesi
Name:Aqp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68428. Aqp3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28CytoplasmicSequence analysisAdd BLAST28
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Topological domaini50 – 53ExtracellularSequence analysis4
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 109CytoplasmicSequence analysisAdd BLAST35
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Topological domaini131 – 157ExtracellularSequence analysisAdd BLAST27
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 188CytoplasmicSequence analysis10
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210 – 244ExtracellularSequence analysisAdd BLAST35
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 292CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000639451 – 292Aquaporin-3Add BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP47862.

Expressioni

Tissue specificityi

Renal medulla and colon. Predominantly in the inner medulla.

Gene expression databases

BgeeiENSRNOG00000009797.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013803.

Structurei

3D structure databases

ProteinModelPortaliP47862.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi83 – 85NPA 13
Motifi215 – 217NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0224. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288287.
HOVERGENiHBG106057.
InParanoidiP47862.
KOiK09876.
PhylomeDBiP47862.
TreeFamiTF313173.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023275. Aquaporin_3.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02015. AQUAPORIN3.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRQKELMNR CGEMLHIRYR LLRQALAECL GTLILVMFGC GSVAQVVLSR
60 70 80 90 100
GTHGGFLTIN LAFGFAVTLA ILVAGQVSGA HLNPAVTFAM CFLAREPWIK
110 120 130 140 150
LPIYTLAQTL GAFLGAGIVF GLYYDAIWAF AGNELVVSGP NGTAGIFATY
160 170 180 190 200
PSGHLDMVNG FFDQFIGTAA LIVCVLAIVD PYNNPVPRGL EAFTVGLVVL
210 220 230 240 250
VIGTSMGFNS GYAVNPARDF GPRLFTALAG WGSEVFTTGQ NWWWVPIVSP
260 270 280 290
LLGSIGGVFV YQLMIGCHLE QPPPSTEAEN VKLAHMKHKE QI
Length:292
Mass (Da):31,384
Last modified:February 1, 1996 - v1
Checksum:i41D9D3119DBA55AD
GO

Sequence cautioni

The sequence BAA04559 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273P → L in AAI27491 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35108 mRNA. Translation: AAA53652.1.
BC127490 mRNA. Translation: AAI27491.1.
D17695 mRNA. Translation: BAA04559.1. Different initiation.
PIRiI59266.
RefSeqiNP_113891.1. NM_031703.1.
UniGeneiRn.11109.

Genome annotation databases

GeneIDi65133.
KEGGirno:65133.
UCSCiRGD:68428. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35108 mRNA. Translation: AAA53652.1.
BC127490 mRNA. Translation: AAI27491.1.
D17695 mRNA. Translation: BAA04559.1. Different initiation.
PIRiI59266.
RefSeqiNP_113891.1. NM_031703.1.
UniGeneiRn.11109.

3D structure databases

ProteinModelPortaliP47862.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013803.

Protein family/group databases

TCDBi1.A.8.9.1. the major intrinsic protein (mip) family.

Proteomic databases

PaxDbiP47862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65133.
KEGGirno:65133.
UCSCiRGD:68428. rat.

Organism-specific databases

CTDi360.
RGDi68428. Aqp3.

Phylogenomic databases

eggNOGiKOG0224. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288287.
HOVERGENiHBG106057.
InParanoidiP47862.
KOiK09876.
PhylomeDBiP47862.
TreeFamiTF313173.

Miscellaneous databases

PROiP47862.

Gene expression databases

BgeeiENSRNOG00000009797.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023275. Aquaporin_3.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02015. AQUAPORIN3.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAQP3_RAT
AccessioniPrimary (citable) accession number: P47862
Secondary accession number(s): A0JPL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.