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Protein

Synaptotagmin-5

Gene

Syt5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca2+-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi139 – 1391Calcium 1By similarity
Metal bindingi139 – 1391Calcium 2By similarity
Metal bindingi145 – 1451Calcium 1By similarity
Metal bindingi197 – 1971Calcium 1By similarity
Metal bindingi197 – 1971Calcium 2By similarity
Metal bindingi198 – 1981Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi199 – 1991Calcium 1By similarity
Metal bindingi199 – 1991Calcium 2By similarity
Metal bindingi199 – 1991Calcium 3By similarity
Metal bindingi202 – 2021Calcium 3By similarity
Metal bindingi205 – 2051Calcium 2By similarity
Metal bindingi205 – 2051Calcium 3By similarity

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: BHF-UCL
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: BHF-UCL
  • syntaxin binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-5
Alternative name(s):
Synaptotagmin IX
Synaptotagmin V
Short name:
SytV
Gene namesi
Name:Syt5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3806. Syt5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424VesicularSequence analysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence analysisAdd
BLAST
Topological domaini46 – 386341CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endosome, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Synaptotagmin-5PRO_0000183953Add
BLAST

Proteomic databases

PaxDbiP47861.
PRIDEiP47861.

PTM databases

iPTMnetiP47861.
PhosphoSiteiP47861.

Expressioni

Tissue specificityi

Expressed in kidney, adipose tissue, lung and heart, as well as at higher levels in brain.

Gene expression databases

GenevisibleiP47861. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with both alpha- and beta-tubulin (PubMed:12966166).By similarity1 Publication

GO - Molecular functioni

  • clathrin binding Source: BHF-UCL
  • syntaxin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi248527. 1 interaction.
STRINGi10116.ENSRNOP00000024547.

Structurei

3D structure databases

ProteinModelPortaliP47861.
SMRiP47861. Positions 108-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 21188C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini253 – 34492C2 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XRME. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiP47861.
KOiK19905.
OMAiMFSEPPT.
OrthoDBiEOG78PV8W.
PhylomeDBiP47861.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
[Graphical view]
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47861-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPEPPTPGS PAPETPPDSS RIRQGAVPAW VLATILLGSG LLVFSSCFCL
60 70 80 90 100
YRKRCRRRMG KKSQAQAQVH LQEVKELGRS YIDKVQPEIE ELDPSPSMPG
110 120 130 140 150
QQVLDKHQLG RLQYSLDYDF QTGQLLVGIL QAEGLAALDL GGSSDPYVSV
160 170 180 190 200
YLLPDKRRRH ETKVHRQTLN PHFGETFAFK VPYVELGGRV LVMAVYDFDR
210 220 230 240 250
FSRNDAIGEV RVPMSSVNLG RPVQAWRELQ VAPKEEQEKL GDICFSLRYV
260 270 280 290 300
PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN
310 320 330 340 350
TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGTAVG
360 370 380
GAGLRHWADM LANPRRPIAQ WHSLRPPDRA RPIPAP
Length:386
Mass (Da):43,127
Last modified:February 1, 1996 - v1
Checksum:i51B881ADD46E3FD6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti182 – 1821P → S.
Natural varianti237 – 2371Missing .

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84884 mRNA. Translation: CAA59311.1.
U26402 mRNA. Translation: AAA81382.1.
BC092198 mRNA. Translation: AAH92198.1.
PIRiI59387.
RefSeqiNP_062223.1. NM_019350.2.
UniGeneiRn.11030.

Genome annotation databases

EnsembliENSRNOT00000024547; ENSRNOP00000024547; ENSRNOG00000018217.
GeneIDi54309.
KEGGirno:54309.
UCSCiRGD:3806. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84884 mRNA. Translation: CAA59311.1.
U26402 mRNA. Translation: AAA81382.1.
BC092198 mRNA. Translation: AAH92198.1.
PIRiI59387.
RefSeqiNP_062223.1. NM_019350.2.
UniGeneiRn.11030.

3D structure databases

ProteinModelPortaliP47861.
SMRiP47861. Positions 108-375.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248527. 1 interaction.
STRINGi10116.ENSRNOP00000024547.

PTM databases

iPTMnetiP47861.
PhosphoSiteiP47861.

Proteomic databases

PaxDbiP47861.
PRIDEiP47861.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024547; ENSRNOP00000024547; ENSRNOG00000018217.
GeneIDi54309.
KEGGirno:54309.
UCSCiRGD:3806. rat.

Organism-specific databases

CTDi6861.
RGDi3806. Syt5.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XRME. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiP47861.
KOiK19905.
OMAiMFSEPPT.
OrthoDBiEOG78PV8W.
PhylomeDBiP47861.
TreeFamiTF315600.

Miscellaneous databases

PROiP47861.

Gene expression databases

GenevisibleiP47861. RN.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
[Graphical view]
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Synaptotagmin V: a novel synaptotagmin isoform expressed in rat brain."
    Craxton M.A., Goedert M.
    FEBS Lett. 361:196-200(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Identification of a nonneuronal isoform of synaptotagmin."
    Hudson A.W., Birnbaum M.J.
    Proc. Natl. Acad. Sci. U.S.A. 92:5895-5899(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Synaptotagmin IX, a possible linker between the perinuclear endocytic recycling compartment and the microtubules."
    Haberman Y., Grimberg E., Fukuda M., Sagi-Eisenberg R.
    J. Cell Sci. 116:4307-4318(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TUBULIN.

Entry informationi

Entry nameiSYT5_RAT
AccessioniPrimary (citable) accession number: P47861
Secondary accession number(s): Q56A28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.