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P47860

- PFKAP_RAT

UniProt

P47860 - PFKAP_RAT

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Protein

ATP-dependent 6-phosphofructokinase, platelet type

Gene

Pfkp

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341ATP; via amide nitrogenUniRule annotation
Metal bindingi128 – 1281Magnesium; catalyticUniRule annotation
Active sitei175 – 1751Proton acceptorUniRule annotation
Binding sitei210 – 2101Substrate; shared with dimeric partnerUniRule annotation
Binding sitei273 – 2731SubstrateUniRule annotation
Binding sitei301 – 3011Substrate; shared with dimeric partnerUniRule annotation
Binding sitei481 – 4811Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei576 – 5761Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei639 – 6391Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei665 – 6651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei744 – 7441Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 982ATPUniRule annotation
Nucleotide bindingi127 – 1304ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB
  2. ATP binding Source: RGD
  3. fructose-6-phosphate binding Source: RGD
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 1,6-bisphosphate metabolic process Source: RGD
  3. fructose 6-phosphate metabolic process Source: RGD
  4. glycolytic process Source: RGD
  5. protein homotetramerization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP47860.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, platelet typeUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PFK-P
Alternative name(s):
6-phosphofructokinase type C
Phosphofructo-1-kinase isozyme C
Short name:
PFK-C
PhosphohexokinaseUniRule annotation
Gene namesi
Name:Pfkp
Synonyms:Pfkc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61893. Pfkp.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788ATP-dependent 6-phosphofructokinase, platelet typePRO_0000112027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Modified residuei386 – 3861PhosphoserineBy similarity
Modified residuei395 – 3951N6-acetyllysineBy similarity
Modified residuei486 – 4861N6-acetyllysineBy similarity
Glycosylationi540 – 5401O-linked (GlcNAc)By similarity
Modified residuei651 – 6511PhosphotyrosineBy similarity
Modified residuei688 – 6881N6-acetyllysineBy similarity

Post-translational modificationi

GlcNAcylation decreases enzyme activity.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP47860.
PRIDEiP47860.

2D gel databases

World-2DPAGE0004:P47860.

PTM databases

PhosphoSiteiP47860.

Expressioni

Tissue specificityi

Expressed at high level in neuroendocrine tissues.

Gene expression databases

GenevestigatoriP47860.

Interactioni

Subunit structurei

Homo- and heterotetramers.UniRule annotation

Protein-protein interaction databases

BioGridi248822. 2 interactions.
IntActiP47860. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP47860.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 399399N-terminal catalytic PFK domain 1Add
BLAST
Regioni173 – 1753Substrate bindingUniRule annotation
Regioni217 – 2193Substrate bindingUniRule annotation
Regioni307 – 3104Substrate bindingUniRule annotation
Regioni400 – 41112Interdomain linkerAdd
BLAST
Regioni412 – 788377C-terminal regulatory PFK domain 2Add
BLAST
Regioni538 – 5425Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni583 – 5853Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni671 – 6744Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiP47860.
KOiK00850.
PhylomeDBiP47860.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47860-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDQDSSTSS TSFPKYLEHL SGDGKAIGVL TSGGDAQGMN AAVRAVVRMG
60 70 80 90 100
IYTGAKVYFI YEGYQGMVDG GSNIVEAKWE CVSSILQVGG TIIGSARCQA
110 120 130 140 150
FRSREGRLKA ACNLVRLGIT NLCVIGGDGS LTGANLFRKE WSGLLEELAK
160 170 180 190 200
NGEIDSDTVK KHAYLNVVGM VGSIDNDFCG TDMTIGTDSA LHRIIEVVDA
210 220 230 240 250
IMTTAQSHQR TFVLEVMGRH CGYLALVSAL ACGADWVFLP ESPPEEGWEE
260 270 280 290 300
EMCLKLSENR ARKKRLNIII VSEGAIDTQN KPITSEKIKE LVVTNLGFDT
310 320 330 340 350
RVTILGHVQR GGTPSAFDRI LASRMGVEAV LALLEATPET PACVVSLRGN
360 370 380 390 400
QAVRLPLMEC VQMTQDVQKA MDERRFDEAV KLRGRSFEGN LNTYKRLAIK
410 420 430 440 450
EPDDKIPKSN CNVAIINVGA PAAGMNAAVR SAVRVGIAEG HKMFAIYDGF
460 470 480 490 500
DGLANGQIKE IGWGDVGGWT GQGGSILGTK RTLPGKYLEK IAEQMHSKNI
510 520 530 540 550
NALLIIGGFE AYLGLLELAA ARNKHEAFCV PMVMVPATVS NNVPGSDFSI
560 570 580 590 600
GADTALNTIT DTCDRIKQSA SGTKRRVFII ETMGGYCGYL ANMGGLAAGA
610 620 630 640 650
DAAYIFEEQF DIRDLQSNVM HLTEKMKTSI QRGLVLRNEN CSVNYTTDFI
660 670 680 690 700
YQLYSEEGKG VFDCRKNVLG HMQQGGAPSP FDRNFGTKIS AKAMEWISAK
710 720 730 740 750
LKGSHGTGKK FVSDDSICVL GIQKRDLLFK PVAELRKATD FEHRIPKQQW
760 770 780
WLKLRPIMKI LAKYEASYDM SDVGKLEPVH NHGELSAI
Length:788
Mass (Da):85,720
Last modified:December 20, 2005 - v2
Checksum:i16FEB963C3297CA6
GO

Sequence cautioni

The sequence AAA17757.1 differs from that shown. Reason: Frameshift at position 27. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 362DA → ES in AAA17757. (PubMed:8106374)Curated
Sequence conflicti51 – 511I → M in AAA17757. (PubMed:8106374)Curated
Sequence conflicti56 – 561K → Q in AAA17757. (PubMed:8106374)Curated
Sequence conflicti111 – 1111A → T in AAA17757. (PubMed:8106374)Curated
Sequence conflicti220 – 2201H → Y in AAA17757. (PubMed:8106374)Curated
Sequence conflicti600 – 6001A → R in AAA17757. (PubMed:8106374)Curated
Sequence conflicti755 – 7551R → L in AAA17757. (PubMed:8106374)Curated
Sequence conflicti758 – 7581M → S in AAA17757. (PubMed:8106374)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088847 mRNA. Translation: AAH88847.1.
L25387 mRNA. Translation: AAA17757.1. Frameshift.
PIRiA53047.
RefSeqiNP_996729.1. NM_206847.1.
UniGeneiRn.2278.

Genome annotation databases

GeneIDi60416.
KEGGirno:60416.
UCSCiRGD:61893. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088847 mRNA. Translation: AAH88847.1 .
L25387 mRNA. Translation: AAA17757.1 . Frameshift.
PIRi A53047.
RefSeqi NP_996729.1. NM_206847.1.
UniGenei Rn.2278.

3D structure databases

ProteinModelPortali P47860.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248822. 2 interactions.
IntActi P47860. 2 interactions.

PTM databases

PhosphoSitei P47860.

2D gel databases

World-2DPAGE 0004:P47860.

Proteomic databases

PaxDbi P47860.
PRIDEi P47860.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 60416.
KEGGi rno:60416.
UCSCi RGD:61893. rat.

Organism-specific databases

CTDi 5214.
RGDi 61893. Pfkp.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
HOVERGENi HBG000976.
InParanoidi P47860.
KOi K00850.
PhylomeDBi P47860.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK P47860.

Miscellaneous databases

NextBioi 612122.

Gene expression databases

Genevestigatori P47860.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "Structure, distribution, and functional expression of the phosphofructokinase C isozyme."
    Gekakis N., Johnson R.C., Jerkins A., Mains R.E., Sul H.S.
    J. Biol. Chem. 269:3348-3355(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-788.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiPFKAP_RAT
AccessioniPrimary (citable) accession number: P47860
Secondary accession number(s): Q5HZX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 20, 2005
Last modified: November 26, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3