6-phosphofructokinase type C - Rattus norvegicus (Rat)

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P47860 (K6PP_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
6-phosphofructokinase type C
EC=2.7.1.11

Alternative name(s):
Phosphofructo-1-kinase isozyme C
Short name=PFK-C
Phosphofructokinase 1
Phosphohexokinase

Gene names

Name:	Pfkp
Synonyms:	Pfkc

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP By similarity.
Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Cofactor	Magnesium.
Enzyme regulation	Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Tissue specificity	Expressed at high level in neuroendocrine tissues.
Post-translational modification	GlcNAcylation decreases enzyme activity By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.
Sequence caution	The sequence AAA17757.1 differs from that shown. Reason: Frameshift at position 27.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 1,6-bisphosphate metabolic process Inferred from direct assay PubMed 2931076. Source: RGD fructose 6-phosphate metabolic process Inferred from direct assay PubMed 2931076. Source: RGD glycolysis Inferred from direct assay PubMed 2931076 PubMed 8255543. Source: RGD protein homotetramerization Inferred from direct assay PubMed 2931076. Source: RGD
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from sequence or structural similarity. Source: UniProtKB ATP binding Inferred from direct assay PubMed 2931076. Source: RGD fructose-6-phosphate binding Inferred from direct assay PubMed 2931076. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 788

788

6-phosphofructokinase type C

PRO_0000112027

Regions

Nucleotide binding

44 – 48

ATP By similarity

Nucleotide binding

202 – 206

ATP By similarity

Nucleotide binding

219 – 235

ATP By similarity

Sites

Active site

175

Proton acceptor By similarity

Metal binding

233

Magnesium; via carbonyl oxygen By similarity

Binding site

210

Substrate By similarity

Binding site

301

Substrate By similarity

Binding site

307

Substrate By similarity

Binding site

310

Substrate By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

386

Phosphoserine By similarity

Modified residue

395

N6-acetyllysine By similarity

Modified residue

486

N6-acetyllysine By similarity

Modified residue

651

Phosphotyrosine By similarity

Modified residue

688

N6-acetyllysine By similarity

Glycosylation

540

O-linked (GlcNAc...) By similarity

Experimental info

Sequence conflict

35 – 36

DA → ES in AAA17757. Ref.2

Sequence conflict

I → M in AAA17757. Ref.2

Sequence conflict

K → Q in AAA17757. Ref.2

Sequence conflict

111

A → T in AAA17757. Ref.2

Sequence conflict

220

H → Y in AAA17757. Ref.2

Sequence conflict

600

A → R in AAA17757. Ref.2

Sequence conflict

755

R → L in AAA17757. Ref.2

Sequence conflict

758

M → S in AAA17757. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P47860 [UniParc].

Last modified December 20, 2005. Version 2.
Checksum: 16FEB963C3297CA6
Show »

FASTA

788

85,720

        10         20         30         40         50         60 
MSDQDSSTSS TSFPKYLEHL SGDGKAIGVL TSGGDAQGMN AAVRAVVRMG IYTGAKVYFI 

        70         80         90        100        110        120 
YEGYQGMVDG GSNIVEAKWE CVSSILQVGG TIIGSARCQA FRSREGRLKA ACNLVRLGIT 

       130        140        150        160        170        180 
NLCVIGGDGS LTGANLFRKE WSGLLEELAK NGEIDSDTVK KHAYLNVVGM VGSIDNDFCG 

       190        200        210        220        230        240 
TDMTIGTDSA LHRIIEVVDA IMTTAQSHQR TFVLEVMGRH CGYLALVSAL ACGADWVFLP 

       250        260        270        280        290        300 
ESPPEEGWEE EMCLKLSENR ARKKRLNIII VSEGAIDTQN KPITSEKIKE LVVTNLGFDT 

       310        320        330        340        350        360 
RVTILGHVQR GGTPSAFDRI LASRMGVEAV LALLEATPET PACVVSLRGN QAVRLPLMEC 

       370        380        390        400        410        420 
VQMTQDVQKA MDERRFDEAV KLRGRSFEGN LNTYKRLAIK EPDDKIPKSN CNVAIINVGA 

       430        440        450        460        470        480 
PAAGMNAAVR SAVRVGIAEG HKMFAIYDGF DGLANGQIKE IGWGDVGGWT GQGGSILGTK 

       490        500        510        520        530        540 
RTLPGKYLEK IAEQMHSKNI NALLIIGGFE AYLGLLELAA ARNKHEAFCV PMVMVPATVS 

       550        560        570        580        590        600 
NNVPGSDFSI GADTALNTIT DTCDRIKQSA SGTKRRVFII ETMGGYCGYL ANMGGLAAGA 

       610        620        630        640        650        660 
DAAYIFEEQF DIRDLQSNVM HLTEKMKTSI QRGLVLRNEN CSVNYTTDFI YQLYSEEGKG 

       670        680        690        700        710        720 
VFDCRKNVLG HMQQGGAPSP FDRNFGTKIS AKAMEWISAK LKGSHGTGKK FVSDDSICVL 

       730        740        750        760        770        780 
GIQKRDLLFK PVAELRKATD FEHRIPKQQW WLKLRPIMKI LAKYEASYDM SDVGKLEPVH 


NHGELSAI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"Structure, distribution, and functional expression of the phosphofructokinase C isozyme." Gekakis N., Johnson R.C., Jerkins A., Mains R.E., Sul H.S. J. Biol. Chem. 269:3348-3355(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-788. Tissue: Hypothalamus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088847 mRNA. Translation: AAH88847.1. L25387 mRNA. Translation: AAA17757.1. Frameshift.
IPI	IPI00231954.
PIR	A53047.
RefSeq	NP_996729.1. NM_206847.1.
UniGene	Rn.2278.
3D structure databases
ProteinModelPortal	P47860.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47860. 2 interactions.
PTM databases
PhosphoSite	P47860.
2D gel databases
World-2DPAGE	0004:P47860.
Proteomic databases
PaxDb	P47860.
PRIDE	P47860.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	60416.
KEGG	rno:60416.
UCSC	RGD:61893. rat.
Organism-specific databases
CTD	5214.
RGD	61893. Pfkp.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000200154.
HOVERGEN	HBG000976.
InParanoid	P47860.
KO	K00850.
OrthoDB	EOG4NS39Z.
Enzyme and pathway databases
SABIO-RK	P47860.
UniPathway	UPA00109; UER00182.
Gene expression databases
ArrayExpress	P47860.
Genevestigator	P47860.
GermOnline	ENSRNOG00000017163. Rattus norvegicus.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	612122.

Entry information

Entry name

K6PP_RAT

Accession

Primary (citable) accession number: P47860
Secondary accession number(s): Q5HZX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 20, 2005
Last modified:	April 3, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents