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P47859

- PFKAP_RABIT

UniProt

P47859 - PFKAP_RABIT

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Protein
ATP-dependent 6-phosphofructokinase, platelet type
Gene
PFKP
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341ATP; via amide nitrogen By similarity
Active sitei175 – 1751Proton acceptor By similarity
Binding sitei210 – 2101Substrate; shared with dimeric partner By similarity
Binding sitei273 – 2731Substrate By similarity
Binding sitei301 – 3011Substrate; shared with dimeric partner By similarity
Binding sitei481 – 4811Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei576 – 5761Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei639 – 6391Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei665 – 6651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei744 – 7441Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 982ATP By similarity
Nucleotide bindingi127 – 1304ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, platelet type (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
PFK-P
Alternative name(s):
6-phosphofructokinase type C
Phosphofructo-1-kinase isozyme C
Short name:
PFK-C
Phosphohexokinase
Gene namesi
Name:PFKP
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 791791ATP-dependent 6-phosphofructokinase, platelet typeUniRule annotation
PRO_0000112026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei12 – 121Phosphoserine; by PKA1 Publication
Modified residuei386 – 3861Phosphoserine By similarity
Modified residuei395 – 3951N6-acetyllysine By similarity
Modified residuei486 – 4861N6-acetyllysine By similarity
Glycosylationi540 – 5401O-linked (GlcNAc) By similarity
Modified residuei651 – 6511Phosphotyrosine By similarity
Modified residuei688 – 6881N6-acetyllysine By similarity

Post-translational modificationi

GlcNAcylation decreases enzyme activity By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP47859.

Interactioni

Subunit structurei

Homo- and heterotetramers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000009500.

Structurei

3D structure databases

ProteinModelPortaliP47859.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 399399N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni173 – 1753Substrate binding By similarity
Regioni217 – 2193Substrate binding By similarity
Regioni307 – 3104Substrate binding By similarity
Regioni400 – 41112Interdomain linkerUniRule annotation
Add
BLAST
Regioni412 – 791380C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni538 – 5425Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni583 – 5853Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni671 – 6744Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47859-1 [UniParc]FASTAAdd to Basket

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MDNKVSASPR GSYRKFLEHL SGAGKAIGVL TSGGDAQGMN AAVRAVVRMG    50
IYVGAKVYFI YEGYQGMVDG GSNIVEANWE SVSSILQVGG TIIGSARSKA 100
FRTREGRLKA ACNLIHRGIT NLCVIGGSGS LTGANIFRME WSGLLEELAQ 150
DGKIDNEAVQ KYAYLNVVGM VGSIDNDFCG TDMTIGTDSA CHRIIEVIDA 200
IMTTAQSHQR TFVLEVMGRH CGYLALVSAL ACGADWVFLP ESPPEEGWEE 250
QMCVKLSENR AQKKRLNIII VAEGAIDTLN RPITSEKIKE LVVTQLGYDT 300
RVTILGHVQR GGTPSAFDRI LASRMGVEAV LALLEATPET PACVVSLSGN 350
HAVRLPLVEC VQMTQEVQKA MDERRFKDAV QLRGRSFENN LNTYKRLAIK 400
LPDDKIQKSN CNVAVINVGA PAAGMNAAVR SAVRVGIADG HKMFAVYDGF 450
DGFAKGQIKE IRWGDVGGWT GQGGSILGTK RILPGKYLEE IATQIRTHNI 500
NAILIIGGFE AYLGLLELSA AREKHEEFCV PMVMVPATVS NNVPGSDFSI 550
GADTALNTIT DTCDRIKQSA SGTKRRVFII ETMGGYCGYL ANMGGLAAGA 600
DAAYIFEEPF DIRDLQSNVE HLTEKMKTTI QRGLVLRNES CSENYTTDFI 650
YQLYSEEGRG VFDCRKNVLG HMQQGGAPSP FDRNFGTKIS ARAMQWITTK 700
LKESPGKGKR FVSDDSICVL GISKRNVLFQ PVAELKNETD FEHRIPKEQW 750
WLKLRPLMKI LAKYKTSYDV SDSGQLVPVR HRGGPEEPAA I 791
Length:791
Mass (Da):86,350
Last modified:February 1, 1996 - v1
Checksum:i3C10A36F229FD8E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01154 mRNA. Translation: AAA17707.1.
PIRiA53206.
RefSeqiNP_001076217.1. NM_001082748.1.
UniGeneiOcu.6209.

Genome annotation databases

GeneIDi100009526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01154 mRNA. Translation: AAA17707.1 .
PIRi A53206.
RefSeqi NP_001076217.1. NM_001082748.1.
UniGenei Ocu.6209.

3D structure databases

ProteinModelPortali P47859.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000009500.

Proteomic databases

PRIDEi P47859.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009526.

Organism-specific databases

CTDi 5214.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
HOVERGENi HBG000976.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and expression of the cDNA for the C isozyme of phosphofructo-1-kinase from rabbit brain."
    Li Y., Valaitis A.P., Latshaw S.P., Kwiatkowska D., Tripathi R.L., Campbell M.C., Kemp R.G.
    J. Biol. Chem. 269:5781-5787(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Brain.
  2. "The sites of phosphorylation of rabbit brain phosphofructo-1-kinase by cyclic AMP-dependent protein kinase."
    Valaitis A.P., Foe L.G., Kwiatkowska D., Latshaw S.P., Kemp R.G.
    Biochim. Biophys. Acta 995:187-194(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 5-17, PHOSPHORYLATION AT SER-12.

Entry informationi

Entry nameiPFKAP_RABIT
AccessioniPrimary (citable) accession number: P47859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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