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P47859

- PFKAP_RABIT

UniProt

P47859 - PFKAP_RABIT

Protein

ATP-dependent 6-phosphofructokinase, platelet type

Gene

PFKP

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341ATP; via amide nitrogenUniRule annotation
    Active sitei175 – 1751Proton acceptorUniRule annotation
    Binding sitei210 – 2101Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei273 – 2731SubstrateUniRule annotation
    Binding sitei301 – 3011Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei481 – 4811Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei576 – 5761Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei639 – 6391Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei665 – 6651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei744 – 7441Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi97 – 982ATPUniRule annotation
    Nucleotide bindingi127 – 1304ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase, platelet typeUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PFK-P
    Alternative name(s):
    6-phosphofructokinase type C
    Phosphofructo-1-kinase isozyme C
    Short name:
    PFK-C
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:PFKP
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 791791ATP-dependent 6-phosphofructokinase, platelet typePRO_0000112026Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei12 – 121Phosphoserine; by PKA1 Publication
    Modified residuei386 – 3861PhosphoserineBy similarity
    Modified residuei395 – 3951N6-acetyllysineBy similarity
    Modified residuei486 – 4861N6-acetyllysineBy similarity
    Glycosylationi540 – 5401O-linked (GlcNAc)By similarity
    Modified residuei651 – 6511PhosphotyrosineBy similarity
    Modified residuei688 – 6881N6-acetyllysineBy similarity

    Post-translational modificationi

    GlcNAcylation decreases enzyme activity.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP47859.

    Interactioni

    Subunit structurei

    Homo- and heterotetramers.UniRule annotation

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000009500.

    Structurei

    3D structure databases

    ProteinModelPortaliP47859.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 399399N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni217 – 2193Substrate bindingUniRule annotation
    Regioni307 – 3104Substrate bindingUniRule annotation
    Regioni400 – 41112Interdomain linkerAdd
    BLAST
    Regioni412 – 791380C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni538 – 5425Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni583 – 5853Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni671 – 6744Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000200154.
    HOVERGENiHBG000976.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47859-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNKVSASPR GSYRKFLEHL SGAGKAIGVL TSGGDAQGMN AAVRAVVRMG    50
    IYVGAKVYFI YEGYQGMVDG GSNIVEANWE SVSSILQVGG TIIGSARSKA 100
    FRTREGRLKA ACNLIHRGIT NLCVIGGSGS LTGANIFRME WSGLLEELAQ 150
    DGKIDNEAVQ KYAYLNVVGM VGSIDNDFCG TDMTIGTDSA CHRIIEVIDA 200
    IMTTAQSHQR TFVLEVMGRH CGYLALVSAL ACGADWVFLP ESPPEEGWEE 250
    QMCVKLSENR AQKKRLNIII VAEGAIDTLN RPITSEKIKE LVVTQLGYDT 300
    RVTILGHVQR GGTPSAFDRI LASRMGVEAV LALLEATPET PACVVSLSGN 350
    HAVRLPLVEC VQMTQEVQKA MDERRFKDAV QLRGRSFENN LNTYKRLAIK 400
    LPDDKIQKSN CNVAVINVGA PAAGMNAAVR SAVRVGIADG HKMFAVYDGF 450
    DGFAKGQIKE IRWGDVGGWT GQGGSILGTK RILPGKYLEE IATQIRTHNI 500
    NAILIIGGFE AYLGLLELSA AREKHEEFCV PMVMVPATVS NNVPGSDFSI 550
    GADTALNTIT DTCDRIKQSA SGTKRRVFII ETMGGYCGYL ANMGGLAAGA 600
    DAAYIFEEPF DIRDLQSNVE HLTEKMKTTI QRGLVLRNES CSENYTTDFI 650
    YQLYSEEGRG VFDCRKNVLG HMQQGGAPSP FDRNFGTKIS ARAMQWITTK 700
    LKESPGKGKR FVSDDSICVL GISKRNVLFQ PVAELKNETD FEHRIPKEQW 750
    WLKLRPLMKI LAKYKTSYDV SDSGQLVPVR HRGGPEEPAA I 791
    Length:791
    Mass (Da):86,350
    Last modified:February 1, 1996 - v1
    Checksum:i3C10A36F229FD8E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01154 mRNA. Translation: AAA17707.1.
    PIRiA53206.
    RefSeqiNP_001076217.1. NM_001082748.1.
    UniGeneiOcu.6209.

    Genome annotation databases

    GeneIDi100009526.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01154 mRNA. Translation: AAA17707.1 .
    PIRi A53206.
    RefSeqi NP_001076217.1. NM_001082748.1.
    UniGenei Ocu.6209.

    3D structure databases

    ProteinModelPortali P47859.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000009500.

    Proteomic databases

    PRIDEi P47859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009526.

    Organism-specific databases

    CTDi 5214.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000200154.
    HOVERGENi HBG000976.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of the cDNA for the C isozyme of phosphofructo-1-kinase from rabbit brain."
      Li Y., Valaitis A.P., Latshaw S.P., Kwiatkowska D., Tripathi R.L., Campbell M.C., Kemp R.G.
      J. Biol. Chem. 269:5781-5787(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Brain.
    2. "The sites of phosphorylation of rabbit brain phosphofructo-1-kinase by cyclic AMP-dependent protein kinase."
      Valaitis A.P., Foe L.G., Kwiatkowska D., Latshaw S.P., Kemp R.G.
      Biochim. Biophys. Acta 995:187-194(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 5-17, PHOSPHORYLATION AT SER-12.

    Entry informationi

    Entry nameiPFKAP_RABIT
    AccessioniPrimary (citable) accession number: P47859
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3