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P47858

- PFKAM_RAT

UniProt

P47858 - PFKAM_RAT

Protein

ATP-dependent 6-phosphofructokinase, muscle type

Gene

Pfkm

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251ATP; via amide nitrogenUniRule annotation
    Metal bindingi119 – 1191Magnesium; catalyticUniRule annotation
    Active sitei166 – 1661Proton acceptorUniRule annotation
    Binding sitei201 – 2011Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei264 – 2641SubstrateUniRule annotation
    Binding sitei292 – 2921Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei471 – 4711Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei566 – 5661Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei629 – 6291Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei655 – 6551Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei735 – 7351Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi88 – 892ATPUniRule annotation
    Nucleotide bindingi118 – 1214ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB
    2. AMP binding Source: RGD
    3. ATP binding Source: RGD
    4. carbohydrate binding Source: RGD
    5. fructose-6-phosphate binding Source: RGD
    6. metal ion binding Source: UniProtKB-KW
    7. phosphofructokinase activity Source: RGD

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. cellular glucose homeostasis Source: RGD
    3. fructose 1,6-bisphosphate metabolic process Source: RGD
    4. fructose 6-phosphate metabolic process Source: RGD
    5. glycolytic process Source: RGD
    6. protein homotetramerization Source: RGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP47858.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase, muscle typeUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PFK-M
    Alternative name(s):
    6-phosphofructokinase type A
    Phosphofructo-1-kinase isozyme A
    Short name:
    PFK-A
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:Pfkm
    Synonyms:Pfk-m
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi68419. Pfkm.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 780779ATP-dependent 6-phosphofructokinase, muscle typePRO_0000112020Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity
    Glycosylationi530 – 5301O-linked (GlcNAc)By similarity
    Modified residuei775 – 7751PhosphoserineBy similarity

    Post-translational modificationi

    GlcNAcylation decreases enzyme activity.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP47858.
    PRIDEiP47858.

    PTM databases

    PhosphoSiteiP47858.

    Expressioni

    Gene expression databases

    GenevestigatoriP47858.

    Interactioni

    Subunit structurei

    Homo- and heterotetramers.

    Protein-protein interaction databases

    BioGridi249268. 1 interaction.
    IntActiP47858. 1 interaction.
    MINTiMINT-89284.

    Structurei

    3D structure databases

    ProteinModelPortaliP47858.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 390389N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni164 – 1663Substrate bindingUniRule annotation
    Regioni208 – 2103Substrate bindingUniRule annotation
    Regioni298 – 3014Substrate bindingUniRule annotation
    Regioni391 – 40111Interdomain linkerAdd
    BLAST
    Regioni402 – 780379C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni528 – 5325Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni573 – 5753Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni661 – 6644Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000200154.
    HOVERGENiHBG000976.
    InParanoidiP47858.
    KOiK00850.
    PhylomeDBiP47858.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P47858-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTHEEHHEAK TLGIGKAIAV LTSGGDAQGM NATVRAVVRV GIFTGLRVFF    50
    VHEGYQGLVD GGEHIREATW ESVSMMLQLG GTVIGSARCK DFREREGRLR 100
    AAHNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLNDLQ KDGKITAEER 150
    TKSSYLNIVF LVGSIDNDFC GTDMTIGTDS ALHRIVEIVD AITTTAQSHQ 200
    RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET 250
    RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ 300
    RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NTAVRLPLME 350
    CVQVTKDVTK AMDEKRFDEA IKLRGRSFMN NWEVYKLLAH VRPPVSKGGL 400
    HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE 450
    AGWSYVGGWT GQGGSKLGTK RTLPKKNLEQ ISANITKYNI QGLVIIGGFE 500
    AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSI GADTALNTIC 550
    TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF 600
    TIRDLQVNVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG 650
    IFDSRKNVLG HMQQGGNPTP FDRNFATKMG AKATNWMSGK IKESYRNGRI 700
    FANTPDSGCV LGMRKRALVF QPVTELKDQT DFEHRIPKEQ WWLKLRPILK 750
    ILAKYEIDLD TSDHAHLEHI SRKRSGEAAV 780
    Length:780
    Mass (Da):85,560
    Last modified:January 23, 2007 - v3
    Checksum:iCECC3995C4F271FA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331T → A in BAA21013. 1 PublicationCurated
    Sequence conflicti46 – 461L → A in BAA21013. 1 PublicationCurated
    Sequence conflicti150 – 1501R → A in BAA21013. 1 PublicationCurated
    Sequence conflicti160 – 1601F → G in BAA21013. 1 PublicationCurated
    Sequence conflicti180 – 1801S → F in BAA21013. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25651 mRNA. Translation: AAC52786.1.
    D21869 mRNA. Translation: BAA21013.1.
    PIRiS71429.
    RefSeqiNP_113903.1. NM_031715.1.
    UniGeneiRn.11004.

    Genome annotation databases

    GeneIDi65152.
    KEGGirno:65152.
    UCSCiRGD:68419. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25651 mRNA. Translation: AAC52786.1 .
    D21869 mRNA. Translation: BAA21013.1 .
    PIRi S71429.
    RefSeqi NP_113903.1. NM_031715.1.
    UniGenei Rn.11004.

    3D structure databases

    ProteinModelPortali P47858.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249268. 1 interaction.
    IntActi P47858. 1 interaction.
    MINTi MINT-89284.

    PTM databases

    PhosphoSitei P47858.

    Proteomic databases

    PaxDbi P47858.
    PRIDEi P47858.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 65152.
    KEGGi rno:65152.
    UCSCi RGD:68419. rat.

    Organism-specific databases

    CTDi 5213.
    RGDi 68419. Pfkm.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000200154.
    HOVERGENi HBG000976.
    InParanoidi P47858.
    KOi K00850.
    PhylomeDBi P47858.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    SABIO-RK P47858.

    Miscellaneous databases

    NextBioi 613989.

    Gene expression databases

    Genevestigatori P47858.

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of expression of phosphofructokinase isoforms in isolated rat pancreatic islets and purified beta cells and cloning and expression of the rat phosphofructokinase-A isoform."
      Ma Z., Ramanadham S., Turk J., Kempe K., Hu Z., Ladenson J.
      Biochim. Biophys. Acta 1308:151-163(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Pancreatic islet.
    2. Ma Z.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 163-170 AND 494-498.
    3. Nakajima H.
      Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-213.
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiPFKAM_RAT
    AccessioniPrimary (citable) accession number: P47858
    Secondary accession number(s): Q63736
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3