6-phosphofructokinase, muscle type - Mus musculus (Mouse)

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P47857 (K6PF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
6-phosphofructokinase, muscle type
EC=2.7.1.11

Alternative name(s):
Phosphofructo-1-kinase isozyme A
Short name=PFK-A
Short name=Phosphofructokinase-M
Phosphofructokinase 1
Phosphohexokinase

Gene names

Name:	Pfkm
Synonyms:	Pfk-m, Pfka

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP.
Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Cofactor	Magnesium.
Enzyme regulation	Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Homotetramer.
Post-translational modification	GlcNAcylation decreases enzyme activity By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glucose homeostasis Inferred from mutant phenotype PubMed 17595219. Source: MGI glycolysis Inferred from direct assay PubMed 2521854. Source: MGI muscle cell homeostasis Inferred from electronic annotation. Source: Compara positive regulation of insulin secretion Inferred from mutant phenotype PubMed 17595219. Source: MGI protein oligomerization Inferred from electronic annotation. Source: Compara
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro apical plasma membrane Inferred from electronic annotation. Source: Compara cytosol Inferred from direct assay PubMed 2521854. Source: MGI
Molecular_function	6-phosphofructokinase activity Inferred from sequence or structural similarity. Source: UniProtKB ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose binding Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 780

779

6-phosphofructokinase, muscle type

PRO_0000112018

Regions

Nucleotide binding

35 – 39

ATP By similarity

Nucleotide binding

193 – 197

ATP By similarity

Nucleotide binding

210 – 226

ATP By similarity

Sites

Active site

166

Proton acceptor By similarity

Metal binding

224

Magnesium; via carbonyl oxygen By similarity

Binding site

201

Substrate By similarity

Binding site

292

Substrate By similarity

Binding site

298

Substrate By similarity

Binding site

301

Substrate By similarity

Amino acid modifications

Modified residue

N-acetylthreonine By similarity

Modified residue

667

Phosphoserine By similarity

Modified residue

775

Phosphoserine By similarity

Glycosylation

530

O-linked (GlcNAc...) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P47857 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7917C7AC108B25C7
Show »

FASTA

780

85,269

        10         20         30         40         50         60 
MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD 

        70         80         90        100        110        120 
GGEHIREATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAHNLVKRGI TNLCVIGGDG 

       130        140        150        160        170        180 
SLTGADTFRS EWSDLLNDLQ KDGKITAEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS 

       190        200        210        220        230        240 
ALHRIVEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE 

       250        260        270        280        290        300 
EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ 

       310        320        330        340        350        360 
RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK 

       370        380        390        400        410        420 
AMDEKRFDEA IKLRGRSFMN NWEVYKLLAH VRPPVSKGGL HTVAVMNVGA PAAGMNAAVR 

       430        440        450        460        470        480 
STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKNLEQ 

       490        500        510        520        530        540 
ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSI 

       550        560        570        580        590        600 
GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF 

       610        620        630        640        650        660 
TIRDLQVNVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG 

       670        680        690        700        710        720 
HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF 

       730        740        750        760        770        780 
QPVTELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI SRKRSGEAAV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization, 5'flanking region and tissue-specific expression of mouse phosphofructokinase C gene." Gunasekera D., Kemp R.G. Gene 260:103-112(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney and Spinal cord.
[3]	"Expression of mouse phosphofructokinase-M gene alternative transcripts: evidence for the conserved two-promoter system." Nakajima H., Noguchi T., Hamaguchi T., Tomita K., Hanafusa T., Kono N., Tanaka T., Kuwajima M., Matsuzawa Y. Biochem. J. 303:449-453(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-212 AND 259-345. Strain: ICR.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 367-374, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF249894 mRNA. Translation: AAF63762.1. AK002711 mRNA. Translation: BAB22303.1. AK049773 mRNA. Translation: BAC33913.1. D21864 Genomic DNA. Translation: BAA21892.1. D21865 mRNA. Translation: BAA21012.1.
IPI	IPI00331541.
PIR	S53317.
RefSeq	NP_001156959.1. NM_001163487.1. NP_001156960.1. NM_001163488.1. NP_067489.3. NM_021514.4.
UniGene	Mm.272582.
3D structure databases
ProteinModelPortal	P47857.
SMR	P47857. Positions 9-756.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47857. 3 interactions.
PTM databases
PhosphoSite	P47857.
Proteomic databases
PaxDb	P47857.
PRIDE	P47857.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000051226; ENSMUSP00000059801; ENSMUSG00000033065. ENSMUST00000163507; ENSMUSP00000132803; ENSMUSG00000033065.
GeneID	18642.
KEGG	mmu:18642.
UCSC	uc007xlv.2. mouse.
Organism-specific databases
CTD	5213.
MGI	MGI:97548. Pfkm.
Phylogenomic databases
eggNOG	COG0205.
GeneTree	ENSGT00390000013209.
HOGENOM	HOG000200154.
HOVERGEN	HBG000976.
InParanoid	P47857.
KO	K00850.
OMA	VYHMASK.
OrthoDB	EOG4RJG0W.
Enzyme and pathway databases
SABIO-RK	P47857.
UniPathway	UPA00109; UER00182.
Gene expression databases
ArrayExpress	P47857.
Bgee	P47857.
CleanEx	MM_PFKM.
Genevestigator	P47857.
GermOnline	ENSMUSG00000033065. Mus musculus.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PFKM. mouse.
NextBio	294630.
PMAP-CutDB	P47857.
SOURCE	Search...

Entry information

Entry name

K6PF_MOUSE

Accession

Primary (citable) accession number: P47857
Secondary accession number(s): O35513, Q543L1, Q9JK94

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 128 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents