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P47857

- PFKAM_MOUSE

UniProt

P47857 - PFKAM_MOUSE

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Protein
ATP-dependent 6-phosphofructokinase, muscle type
Gene
Pfkm, Pfk-m, Pfka
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251ATP; via amide nitrogen By similarity
Metal bindingi119 – 1191Magnesium; catalytic By similarity
Active sitei166 – 1661Proton acceptor By similarity
Binding sitei201 – 2011Substrate; shared with dimeric partner By similarity
Binding sitei264 – 2641Substrate By similarity
Binding sitei292 – 2921Substrate; shared with dimeric partner By similarity
Binding sitei471 – 4711Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei566 – 5661Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei629 – 6291Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei655 – 6551Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei735 – 7351Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 892ATP By similarity
Nucleotide bindingi118 – 1214ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. fructose binding Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW
  5. phosphofructokinase activity Source: MGI
  6. protein binding Source: MGI
  7. protein homodimerization activity Source: MGI

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glucose homeostasis Source: MGI
  4. glycolytic process Source: MGI
  5. glycolytic process through fructose-6-phosphate Source: MGI
  6. muscle cell cellular homeostasis Source: Ensembl
  7. positive regulation of insulin secretion Source: MGI
  8. protein oligomerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP47857.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, muscle type (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
PFK-M
Alternative name(s):
6-phosphofructokinase type A
Phosphofructo-1-kinase isozyme A
Short name:
PFK-A
Phosphohexokinase
Gene namesi
Name:Pfkm
Synonyms:Pfk-m, Pfka
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:97548. Pfkm.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
  2. apical plasma membrane Source: Ensembl
  3. cytosol Source: MGI
  4. sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 780779ATP-dependent 6-phosphofructokinase, muscle typeUniRule annotation
PRO_0000112018Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine By similarity
Glycosylationi530 – 5301O-linked (GlcNAc) By similarity
Modified residuei667 – 6671Phosphoserine By similarity
Modified residuei775 – 7751Phosphoserine By similarity

Post-translational modificationi

GlcNAcylation decreases enzyme activity By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP47857.
PaxDbiP47857.
PRIDEiP47857.

PTM databases

PhosphoSiteiP47857.

Miscellaneous databases

PMAP-CutDBP47857.

Expressioni

Gene expression databases

ArrayExpressiP47857.
BgeeiP47857.
CleanExiMM_PFKM.
GenevestigatoriP47857.

Interactioni

Subunit structurei

Homo- and heterotetramers.

Protein-protein interaction databases

BioGridi202125. 2 interactions.
IntActiP47857. 4 interactions.
MINTiMINT-1869891.

Structurei

3D structure databases

ProteinModelPortaliP47857.
SMRiP47857. Positions 9-756.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 390389N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni164 – 1663Substrate binding By similarity
Regioni208 – 2103Substrate binding By similarity
Regioni298 – 3014Substrate binding By similarity
Regioni391 – 40111Interdomain linkerUniRule annotation
Add
BLAST
Regioni402 – 780379C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni528 – 5325Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni573 – 5753Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni661 – 6644Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiP47857.
KOiK00850.
OMAiVYHMASK.
OrthoDBiEOG7ZSHV5.
PhylomeDBiP47857.
TreeFamiTF300411.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47857-1 [UniParc]FASTAAdd to Basket

« Hide

MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF    50
VHEGYQGLVD GGEHIREATW ESVSMMLQLG GTVIGSARCK DFREREGRLR 100
AAHNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLNDLQ KDGKITAEEA 150
TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIVEIVD AITTTAQSHQ 200
RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET 250
RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ 300
RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME 350
CVQVTKDVTK AMDEKRFDEA IKLRGRSFMN NWEVYKLLAH VRPPVSKGGL 400
HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE 450
AGWSYVGGWT GQGGSKLGTK RTLPKKNLEQ ISANITKFNI QGLVIIGGFE 500
AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSI GADTALNTIC 550
TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF 600
TIRDLQVNVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG 650
IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI 700
FANTPDSGCV LGMRKRALVF QPVTELKDQT DFEHRIPKEQ WWLKLRPILK 750
ILAKYEIDLD TSDHAHLEHI SRKRSGEAAV 780
Length:780
Mass (Da):85,269
Last modified:January 23, 2007 - v3
Checksum:i7917C7AC108B25C7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF249894 mRNA. Translation: AAF63762.1.
AK002711 mRNA. Translation: BAB22303.1.
AK049773 mRNA. Translation: BAC33913.1.
D21864 Genomic DNA. Translation: BAA21892.1.
D21865 mRNA. Translation: BAA21012.1.
CCDSiCCDS27786.1.
PIRiS53317.
RefSeqiNP_001156959.1. NM_001163487.1.
NP_001156960.1. NM_001163488.1.
NP_067489.3. NM_021514.4.
XP_006520665.1. XM_006520602.1.
UniGeneiMm.272582.

Genome annotation databases

EnsembliENSMUST00000051226; ENSMUSP00000059801; ENSMUSG00000033065.
ENSMUST00000163507; ENSMUSP00000132803; ENSMUSG00000033065.
GeneIDi18642.
KEGGimmu:18642.
UCSCiuc007xlv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF249894 mRNA. Translation: AAF63762.1 .
AK002711 mRNA. Translation: BAB22303.1 .
AK049773 mRNA. Translation: BAC33913.1 .
D21864 Genomic DNA. Translation: BAA21892.1 .
D21865 mRNA. Translation: BAA21012.1 .
CCDSi CCDS27786.1.
PIRi S53317.
RefSeqi NP_001156959.1. NM_001163487.1.
NP_001156960.1. NM_001163488.1.
NP_067489.3. NM_021514.4.
XP_006520665.1. XM_006520602.1.
UniGenei Mm.272582.

3D structure databases

ProteinModelPortali P47857.
SMRi P47857. Positions 9-756.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202125. 2 interactions.
IntActi P47857. 4 interactions.
MINTi MINT-1869891.

PTM databases

PhosphoSitei P47857.

Proteomic databases

MaxQBi P47857.
PaxDbi P47857.
PRIDEi P47857.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000051226 ; ENSMUSP00000059801 ; ENSMUSG00000033065 .
ENSMUST00000163507 ; ENSMUSP00000132803 ; ENSMUSG00000033065 .
GeneIDi 18642.
KEGGi mmu:18642.
UCSCi uc007xlv.2. mouse.

Organism-specific databases

CTDi 5213.
MGIi MGI:97548. Pfkm.

Phylogenomic databases

eggNOGi COG0205.
GeneTreei ENSGT00390000013209.
HOGENOMi HOG000200154.
HOVERGENi HBG000976.
InParanoidi P47857.
KOi K00850.
OMAi VYHMASK.
OrthoDBi EOG7ZSHV5.
PhylomeDBi P47857.
TreeFami TF300411.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK P47857.

Miscellaneous databases

ChiTaRSi PFKM. mouse.
NextBioi 294630.
PMAP-CutDB P47857.
PROi P47857.
SOURCEi Search...

Gene expression databases

ArrayExpressi P47857.
Bgeei P47857.
CleanExi MM_PFKM.
Genevestigatori P47857.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization, 5'flanking region and tissue-specific expression of mouse phosphofructokinase C gene."
    Gunasekera D., Kemp R.G.
    Gene 260:103-112(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney and Spinal cord.
  3. "Expression of mouse phosphofructokinase-M gene alternative transcripts: evidence for the conserved two-promoter system."
    Nakajima H., Noguchi T., Hamaguchi T., Tomita K., Hanafusa T., Kono N., Tanaka T., Kuwajima M., Matsuzawa Y.
    Biochem. J. 303:449-453(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-212 AND 259-345.
    Strain: ICR.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 367-374, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiPFKAM_MOUSE
AccessioniPrimary (citable) accession number: P47857
Secondary accession number(s): O35513, Q543L1, Q9JK94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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