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P47856

- GFPT1_MOUSE

UniProt

P47856 - GFPT1_MOUSE

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Gene

Gfpt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.1 Publication

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei442 – 4421SubstrateBy similarity
    Binding sitei582 – 5821SubstrateBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: Ensembl
    2. carbohydrate binding Source: Ensembl
    3. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. cellular response to insulin stimulus Source: Ensembl
    3. circadian regulation of gene expression Source: UniProtKB
    4. fructose 6-phosphate metabolic process Source: Ensembl
    5. glucosamine biosynthetic process Source: Ensembl
    6. glutamine metabolic process Source: UniProtKB-KW
    7. negative regulation of glycogen biosynthetic process Source: Ensembl
    8. protein homotetramerization Source: Ensembl
    9. response to sucrose Source: Ensembl
    10. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Biological rhythms

    Enzyme and pathway databases

    ReactomeiREACT_106572. XBP1(S) activates chaperone genes.
    REACT_198714. Synthesis of UDP-N-acetyl-glucosamine.
    UniPathwayiUPA00113; UER00528.

    Protein family/group databases

    MEROPSiC44.970.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (EC:2.6.1.16)
    Alternative name(s):
    D-fructose-6-phosphate amidotransferase 1
    Glutamine:fructose-6-phosphate amidotransferase 1
    Short name:
    GFAT 1
    Short name:
    GFAT1
    Hexosephosphate aminotransferase 1
    Gene namesi
    Name:Gfpt1
    Synonyms:Gfpt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:95698. Gfpt1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 697696Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1PRO_0000135281Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei259 – 2591Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47856.
    PaxDbiP47856.
    PRIDEiP47856.

    PTM databases

    PhosphoSiteiP47856.

    Expressioni

    Tissue specificityi

    Isoform 1 is predominantly expressed in hindlimb muscle and is also expressed weakly in the heart. Seems to be selectively expressed in striated muscle.1 Publication

    Inductioni

    Expression in the liver oscillates in an ultradian manner (with a 12 hour period/cycle).1 Publication

    Gene expression databases

    ArrayExpressiP47856.
    BgeeiP47856.
    CleanExiMM_GFPT1.
    GenevestigatoriP47856.

    Interactioni

    Subunit structurei

    Homotetramer, may also exist as homodimers.By similarity

    Protein-protein interaction databases

    BioGridi199902. 1 interaction.
    IntActiP47856. 2 interactions.
    MINTiMINT-4096028.

    Structurei

    3D structure databases

    ProteinModelPortaliP47856.
    SMRiP47856. Positions 2-697.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 303302Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini375 – 514140SIS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini546 – 687142SIS 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni311 – 678368IsomeraseBy similarityAdd
    BLAST
    Regioni392 – 3932Substrate-bindingBy similarity
    Regioni437 – 4393Substrate-bindingBy similarity

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
    Contains 2 SIS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    GeneTreeiENSGT00390000010049.
    HOGENOMiHOG000258898.
    HOVERGENiHBG051724.
    KOiK00820.
    OMAiMYDNRDS.
    OrthoDBiEOG73NG2S.
    PhylomeDBiP47856.
    TreeFamiTF300864.

    Family and domain databases

    Gene3Di3.60.20.10. 2 hits.
    InterProiIPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PfamiPF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 2 hits.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P47856-1) [UniParc]FASTAAdd to Basket

    Also known as: GFAT1m

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW    50
    EANACKIQLI KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG 100
    EPNPVNSHPQ RSDKNNEFIV IHNGIITNYK DLKKFLESKG YDFESETDTE 150
    TIAKLVKYMY DNWESQDVSF TTLVERVIQQ LEGAFALVFK SVHFPGQAVG 200
    TRRGSPLLIG VRSEHKLSTD HIPILYRTAR TQIGSTWWGS QAERGKDKKG 250
    SCGLSRVDST TCLFPVEEKA VEYYFASDAS AVIEHTNRVI FLEDDDVAAV 300
    VDGRLSIHRI KRTAGDHPGR AVQTLQMELQ QIMKGNFSSF MQKEIFEQPE 350
    SVVNTMRGRV NFDDYTVNLG GLKDHIKEIQ RCRRLILIAC GTSYHAGVAT 400
    RQVLEELTEL PVMVELASDF LDRNTPVFRD DVCFFISQSG ETADTLMGLR 450
    YCKERGALTV GITNTVGSSI SRETDCGVHI NAGPEIGVAS TKAYTSQFVS 500
    LVMFALMMCD DRISMQERRK EIMLGLKRLP DLIKEVLSMD DEIQKLATEL 550
    YHQKSVLIMG RGYHYATCLE GALKIKEITY MHSEGILAGE LKHGPLALVD 600
    KLMPVIMIIM RDHTYAKCQN ALQQVVARQG RPVVICDKED TETIKNTKRT 650
    IKVPHSVDCL QGILSVIPLQ LLAFHLAVLR GYDVDFPRNL AKSVTVE 697
    Length:697
    Mass (Da):78,539
    Last modified:January 23, 2007 - v3
    Checksum:iB7B01F6CD4CEB655
    GO
    Isoform 2 (identifier: P47856-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         229-244: Missing.

    Show »
    Length:681
    Mass (Da):76,723
    Checksum:iBE358A1054107D0B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti99 – 991H → T in AAH10516. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei229 – 24416Missing in isoform 2. 3 PublicationsVSP_007498Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00932 mRNA. Translation: AAC27348.1.
    AF334736 mRNA. Translation: AAK15341.1.
    AK019852 mRNA. Translation: BAB31882.1.
    BC002283 mRNA. Translation: AAH02283.1.
    BC010516 mRNA. Translation: AAH10516.1.
    BC050762 mRNA. Translation: AAH50762.1.
    CCDSiCCDS39545.1. [P47856-2]
    PIRiI53743.
    RefSeqiNP_038556.1. NM_013528.3. [P47856-2]
    UniGeneiMm.19893.

    Genome annotation databases

    EnsembliENSMUST00000032057; ENSMUSP00000032057; ENSMUSG00000029992. [P47856-1]
    ENSMUST00000113658; ENSMUSP00000109288; ENSMUSG00000029992. [P47856-2]
    GeneIDi14583.
    KEGGimmu:14583.
    UCSCiuc009csz.2. mouse. [P47856-2]
    uc009cta.2. mouse. [P47856-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00932 mRNA. Translation: AAC27348.1 .
    AF334736 mRNA. Translation: AAK15341.1 .
    AK019852 mRNA. Translation: BAB31882.1 .
    BC002283 mRNA. Translation: AAH02283.1 .
    BC010516 mRNA. Translation: AAH10516.1 .
    BC050762 mRNA. Translation: AAH50762.1 .
    CCDSi CCDS39545.1. [P47856-2 ]
    PIRi I53743.
    RefSeqi NP_038556.1. NM_013528.3. [P47856-2 ]
    UniGenei Mm.19893.

    3D structure databases

    ProteinModelPortali P47856.
    SMRi P47856. Positions 2-697.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199902. 1 interaction.
    IntActi P47856. 2 interactions.
    MINTi MINT-4096028.

    Protein family/group databases

    MEROPSi C44.970.

    PTM databases

    PhosphoSitei P47856.

    Proteomic databases

    MaxQBi P47856.
    PaxDbi P47856.
    PRIDEi P47856.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032057 ; ENSMUSP00000032057 ; ENSMUSG00000029992 . [P47856-1 ]
    ENSMUST00000113658 ; ENSMUSP00000109288 ; ENSMUSG00000029992 . [P47856-2 ]
    GeneIDi 14583.
    KEGGi mmu:14583.
    UCSCi uc009csz.2. mouse. [P47856-2 ]
    uc009cta.2. mouse. [P47856-1 ]

    Organism-specific databases

    CTDi 2673.
    MGIi MGI:95698. Gfpt1.

    Phylogenomic databases

    eggNOGi COG0449.
    GeneTreei ENSGT00390000010049.
    HOGENOMi HOG000258898.
    HOVERGENi HBG051724.
    KOi K00820.
    OMAi MYDNRDS.
    OrthoDBi EOG73NG2S.
    PhylomeDBi P47856.
    TreeFami TF300864.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00528 .
    Reactomei REACT_106572. XBP1(S) activates chaperone genes.
    REACT_198714. Synthesis of UDP-N-acetyl-glucosamine.

    Miscellaneous databases

    NextBioi 286318.
    PROi P47856.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P47856.
    Bgeei P47856.
    CleanExi MM_GFPT1.
    Genevestigatori P47856.

    Family and domain databases

    Gene3Di 3.60.20.10. 2 hits.
    InterProi IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    Pfami PF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 2 hits.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The murine glutamine:fructose-6-phosphate amidotransferase-encoding cDNA sequence."
      Sayeski P.P., Paterson A.J., Kudlow J.E.
      Gene 140:289-290(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: Swiss.
    2. "A novel variant of glutamine:fructose-6-phosphate amidotransferase-1 (GFAT1) mRNA is selectively expressed in striated muscle."
      DeHaven J.E., Robinson K.A., Nelson B.A., Buse M.G.
      Diabetes 50:2419-2424(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Skeletal muscle.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Mammary gland, Salivary gland and Testis.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination."
      Li M.D., Ruan H.B., Hughes M.E., Lee J.S., Singh J.P., Jones S.P., Nitabach M.N., Yang X.
      Cell Metab. 17:303-310(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiGFPT1_MOUSE
    AccessioniPrimary (citable) accession number: P47856
    Secondary accession number(s): Q91XG9, Q99LP7, Q99MJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 125 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3