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P47856

- GFPT1_MOUSE

UniProt

P47856 - GFPT1_MOUSE

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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Gene

Gfpt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.1 Publication

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei442 – 4421SubstrateBy similarity
Binding sitei582 – 5821SubstrateBy similarity

GO - Molecular functioni

  1. amino acid binding Source: Ensembl
  2. carbohydrate binding Source: Ensembl
  3. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. cellular response to insulin stimulus Source: Ensembl
  3. circadian regulation of gene expression Source: UniProtKB
  4. fructose 6-phosphate metabolic process Source: Ensembl
  5. glucosamine biosynthetic process Source: Ensembl
  6. glutamine metabolic process Source: UniProtKB-KW
  7. negative regulation of glycogen biosynthetic process Source: Ensembl
  8. protein homotetramerization Source: Ensembl
  9. response to sucrose Source: Ensembl
  10. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Biological rhythms

Enzyme and pathway databases

ReactomeiREACT_106572. XBP1(S) activates chaperone genes.
REACT_198714. Synthesis of UDP-N-acetyl-glucosamine.
UniPathwayiUPA00113; UER00528.

Protein family/group databases

MEROPSiC44.970.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 1
Glutamine:fructose-6-phosphate amidotransferase 1
Short name:
GFAT 1
Short name:
GFAT1
Hexosephosphate aminotransferase 1
Gene namesi
Name:Gfpt1
Synonyms:Gfpt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:95698. Gfpt1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 697696Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1PRO_0000135281Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei259 – 2591Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47856.
PaxDbiP47856.
PRIDEiP47856.

PTM databases

PhosphoSiteiP47856.

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in hindlimb muscle and is also expressed weakly in the heart. Seems to be selectively expressed in striated muscle.1 Publication

Inductioni

Expression in the liver oscillates in an ultradian manner (with a 12 hour period/cycle).1 Publication

Gene expression databases

BgeeiP47856.
CleanExiMM_GFPT1.
ExpressionAtlasiP47856. baseline and differential.
GenevestigatoriP47856.

Interactioni

Subunit structurei

Homotetramer, may also exist as homodimers.By similarity

Protein-protein interaction databases

BioGridi199902. 1 interaction.
IntActiP47856. 2 interactions.
MINTiMINT-4096028.

Structurei

3D structure databases

ProteinModelPortaliP47856.
SMRiP47856. Positions 16-230, 272-697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 303302Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini375 – 514140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini546 – 687142SIS 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni311 – 678368IsomeraseBy similarityAdd
BLAST
Regioni392 – 3932Substrate-bindingBy similarity
Regioni437 – 4393Substrate-bindingBy similarity

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiP47856.
KOiK00820.
OMAiMYDNRDS.
OrthoDBiEOG73NG2S.
PhylomeDBiP47856.
TreeFamiTF300864.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P47856-1) [UniParc]FASTAAdd to Basket

Also known as: GFAT1m

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW
60 70 80 90 100
EANACKIQLI KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG
110 120 130 140 150
EPNPVNSHPQ RSDKNNEFIV IHNGIITNYK DLKKFLESKG YDFESETDTE
160 170 180 190 200
TIAKLVKYMY DNWESQDVSF TTLVERVIQQ LEGAFALVFK SVHFPGQAVG
210 220 230 240 250
TRRGSPLLIG VRSEHKLSTD HIPILYRTAR TQIGSTWWGS QAERGKDKKG
260 270 280 290 300
SCGLSRVDST TCLFPVEEKA VEYYFASDAS AVIEHTNRVI FLEDDDVAAV
310 320 330 340 350
VDGRLSIHRI KRTAGDHPGR AVQTLQMELQ QIMKGNFSSF MQKEIFEQPE
360 370 380 390 400
SVVNTMRGRV NFDDYTVNLG GLKDHIKEIQ RCRRLILIAC GTSYHAGVAT
410 420 430 440 450
RQVLEELTEL PVMVELASDF LDRNTPVFRD DVCFFISQSG ETADTLMGLR
460 470 480 490 500
YCKERGALTV GITNTVGSSI SRETDCGVHI NAGPEIGVAS TKAYTSQFVS
510 520 530 540 550
LVMFALMMCD DRISMQERRK EIMLGLKRLP DLIKEVLSMD DEIQKLATEL
560 570 580 590 600
YHQKSVLIMG RGYHYATCLE GALKIKEITY MHSEGILAGE LKHGPLALVD
610 620 630 640 650
KLMPVIMIIM RDHTYAKCQN ALQQVVARQG RPVVICDKED TETIKNTKRT
660 670 680 690
IKVPHSVDCL QGILSVIPLQ LLAFHLAVLR GYDVDFPRNL AKSVTVE
Length:697
Mass (Da):78,539
Last modified:January 23, 2007 - v3
Checksum:iB7B01F6CD4CEB655
GO
Isoform 2 (identifier: P47856-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     229-244: Missing.

Show »
Length:681
Mass (Da):76,723
Checksum:iBE358A1054107D0B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991H → T in AAH10516. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei229 – 24416Missing in isoform 2. 3 PublicationsVSP_007498Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00932 mRNA. Translation: AAC27348.1.
AF334736 mRNA. Translation: AAK15341.1.
AK019852 mRNA. Translation: BAB31882.1.
BC002283 mRNA. Translation: AAH02283.1.
BC010516 mRNA. Translation: AAH10516.1.
BC050762 mRNA. Translation: AAH50762.1.
CCDSiCCDS39545.1. [P47856-2]
PIRiI53743.
RefSeqiNP_038556.1. NM_013528.3. [P47856-2]
UniGeneiMm.19893.

Genome annotation databases

EnsembliENSMUST00000032057; ENSMUSP00000032057; ENSMUSG00000029992. [P47856-1]
ENSMUST00000113658; ENSMUSP00000109288; ENSMUSG00000029992. [P47856-2]
GeneIDi14583.
KEGGimmu:14583.
UCSCiuc009csz.2. mouse. [P47856-2]
uc009cta.2. mouse. [P47856-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00932 mRNA. Translation: AAC27348.1 .
AF334736 mRNA. Translation: AAK15341.1 .
AK019852 mRNA. Translation: BAB31882.1 .
BC002283 mRNA. Translation: AAH02283.1 .
BC010516 mRNA. Translation: AAH10516.1 .
BC050762 mRNA. Translation: AAH50762.1 .
CCDSi CCDS39545.1. [P47856-2 ]
PIRi I53743.
RefSeqi NP_038556.1. NM_013528.3. [P47856-2 ]
UniGenei Mm.19893.

3D structure databases

ProteinModelPortali P47856.
SMRi P47856. Positions 16-230, 272-697.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199902. 1 interaction.
IntActi P47856. 2 interactions.
MINTi MINT-4096028.

Protein family/group databases

MEROPSi C44.970.

PTM databases

PhosphoSitei P47856.

Proteomic databases

MaxQBi P47856.
PaxDbi P47856.
PRIDEi P47856.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032057 ; ENSMUSP00000032057 ; ENSMUSG00000029992 . [P47856-1 ]
ENSMUST00000113658 ; ENSMUSP00000109288 ; ENSMUSG00000029992 . [P47856-2 ]
GeneIDi 14583.
KEGGi mmu:14583.
UCSCi uc009csz.2. mouse. [P47856-2 ]
uc009cta.2. mouse. [P47856-1 ]

Organism-specific databases

CTDi 2673.
MGIi MGI:95698. Gfpt1.

Phylogenomic databases

eggNOGi COG0449.
GeneTreei ENSGT00390000010049.
HOGENOMi HOG000258898.
HOVERGENi HBG051724.
InParanoidi P47856.
KOi K00820.
OMAi MYDNRDS.
OrthoDBi EOG73NG2S.
PhylomeDBi P47856.
TreeFami TF300864.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00528 .
Reactomei REACT_106572. XBP1(S) activates chaperone genes.
REACT_198714. Synthesis of UDP-N-acetyl-glucosamine.

Miscellaneous databases

NextBioi 286318.
PROi P47856.
SOURCEi Search...

Gene expression databases

Bgeei P47856.
CleanExi MM_GFPT1.
ExpressionAtlasi P47856. baseline and differential.
Genevestigatori P47856.

Family and domain databases

Gene3Di 3.60.20.10. 2 hits.
InterProi IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
Pfami PF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 2 hits.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The murine glutamine:fructose-6-phosphate amidotransferase-encoding cDNA sequence."
    Sayeski P.P., Paterson A.J., Kudlow J.E.
    Gene 140:289-290(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Swiss.
  2. "A novel variant of glutamine:fructose-6-phosphate amidotransferase-1 (GFAT1) mRNA is selectively expressed in striated muscle."
    DeHaven J.E., Robinson K.A., Nelson B.A., Buse M.G.
    Diabetes 50:2419-2424(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Skeletal muscle.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary gland, Salivary gland and Testis.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination."
    Li M.D., Ruan H.B., Hughes M.E., Lee J.S., Singh J.P., Jones S.P., Nitabach M.N., Yang X.
    Cell Metab. 17:303-310(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiGFPT1_MOUSE
AccessioniPrimary (citable) accession number: P47856
Secondary accession number(s): Q91XG9, Q99LP7, Q99MJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3