Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Gene

Gfpt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.1 Publication

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.By similarity

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Gfpt2), Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Gfpt1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei442SubstrateBy similarity1
Binding sitei593SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processBiological rhythms

Enzyme and pathway databases

ReactomeiR-MMU-446210 Synthesis of UDP-N-acetyl-glucosamine
UniPathwayiUPA00113; UER00528

Protein family/group databases

MEROPSiC44.970

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (EC:2.6.1.16By similarity)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 1
Glutamine:fructose-6-phosphate amidotransferase 1
Short name:
GFAT 1
Short name:
GFAT1
Hexosephosphate aminotransferase 1
Gene namesi
Name:Gfpt1
Synonyms:Gfpt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95698 Gfpt1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001352812 – 697Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1Add BLAST696

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei259PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47856
PaxDbiP47856
PeptideAtlasiP47856
PRIDEiP47856

PTM databases

iPTMnetiP47856
PhosphoSitePlusiP47856

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in hindlimb muscle and is also expressed weakly in the heart. Seems to be selectively expressed in striated muscle.1 Publication

Inductioni

Expression in the liver oscillates in an ultradian manner (with a 12 hour period/cycle).1 Publication

Gene expression databases

BgeeiENSMUSG00000029992
CleanExiMM_GFPT1
ExpressionAtlasiP47856 baseline and differential
GenevisibleiP47856 MM

Interactioni

Subunit structurei

Homotetramer, may also exist as homodimers.By similarity

Protein-protein interaction databases

BioGridi1999023 interactors.
IntActiP47856 3 interactors.
MINTiP47856
STRINGi10090.ENSMUSP00000109288

Structurei

3D structure databases

ProteinModelPortaliP47856
SMRiP47856
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 303Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST302
Domaini375 – 514SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini546 – 687SIS 2PROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni311 – 678IsomeraseBy similarityAdd BLAST368
Regioni392 – 393Substrate-bindingBy similarity2
Regioni437 – 439Substrate-bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiKOG1268 Eukaryota
COG0449 LUCA
GeneTreeiENSGT00390000010049
HOGENOMiHOG000258898
HOVERGENiHBG051724
InParanoidiP47856
KOiK00820
OMAiASEYRYA
OrthoDBiEOG091G02N5
PhylomeDBiP47856
TreeFamiTF300864

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 2 hits
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P47856-1) [UniParc]FASTAAdd to basket
Also known as: GFAT1m

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW
60 70 80 90 100
EANACKIQLI KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG
110 120 130 140 150
EPNPVNSHPQ RSDKNNEFIV IHNGIITNYK DLKKFLESKG YDFESETDTE
160 170 180 190 200
TIAKLVKYMY DNWESQDVSF TTLVERVIQQ LEGAFALVFK SVHFPGQAVG
210 220 230 240 250
TRRGSPLLIG VRSEHKLSTD HIPILYRTAR TQIGSTWWGS QAERGKDKKG
260 270 280 290 300
SCGLSRVDST TCLFPVEEKA VEYYFASDAS AVIEHTNRVI FLEDDDVAAV
310 320 330 340 350
VDGRLSIHRI KRTAGDHPGR AVQTLQMELQ QIMKGNFSSF MQKEIFEQPE
360 370 380 390 400
SVVNTMRGRV NFDDYTVNLG GLKDHIKEIQ RCRRLILIAC GTSYHAGVAT
410 420 430 440 450
RQVLEELTEL PVMVELASDF LDRNTPVFRD DVCFFISQSG ETADTLMGLR
460 470 480 490 500
YCKERGALTV GITNTVGSSI SRETDCGVHI NAGPEIGVAS TKAYTSQFVS
510 520 530 540 550
LVMFALMMCD DRISMQERRK EIMLGLKRLP DLIKEVLSMD DEIQKLATEL
560 570 580 590 600
YHQKSVLIMG RGYHYATCLE GALKIKEITY MHSEGILAGE LKHGPLALVD
610 620 630 640 650
KLMPVIMIIM RDHTYAKCQN ALQQVVARQG RPVVICDKED TETIKNTKRT
660 670 680 690
IKVPHSVDCL QGILSVIPLQ LLAFHLAVLR GYDVDFPRNL AKSVTVE
Length:697
Mass (Da):78,539
Last modified:January 23, 2007 - v3
Checksum:iB7B01F6CD4CEB655
GO
Isoform 2 (identifier: P47856-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     229-244: Missing.

Show »
Length:681
Mass (Da):76,723
Checksum:iBE358A1054107D0B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99H → T in AAH10516 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007498229 – 244Missing in isoform 2. 3 PublicationsAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00932 mRNA Translation: AAC27348.1
AF334736 mRNA Translation: AAK15341.1
AK019852 mRNA Translation: BAB31882.1
BC002283 mRNA Translation: AAH02283.1
BC010516 mRNA Translation: AAH10516.1
BC050762 mRNA Translation: AAH50762.1
CCDSiCCDS39545.1 [P47856-2]
PIRiI53743
RefSeqiNP_038556.1, NM_013528.3 [P47856-2]
UniGeneiMm.19893

Genome annotation databases

EnsembliENSMUST00000032057; ENSMUSP00000032057; ENSMUSG00000029992 [P47856-1]
ENSMUST00000113658; ENSMUSP00000109288; ENSMUSG00000029992 [P47856-2]
GeneIDi14583
KEGGimmu:14583
UCSCiuc009csz.2 mouse [P47856-2]
uc009cta.2 mouse [P47856-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGFPT1_MOUSE
AccessioniPrimary (citable) accession number: P47856
Secondary accession number(s): Q91XG9, Q99LP7, Q99MJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome