P47849 (RF1_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1
Short name=RF-1

Gene names

Name:	prfA
Ordered Locus Names:	CBU_1965

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP-Rule MF_00093
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP-Rule MF_00093
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Peptide chain release factor 1 HAMAP-Rule MF_00093		PRO_0000177664
Amino acid modifications
	Modified residue	237	1	N5-methylglutamine By similarity
Experimental info
	Sequence conflict	99	1	K → N in CAA55563. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47849 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: EDB0A087C408F257	FASTA	361	40,763
	10 20 30 40 50 60 MKPSLIEKLK TLTYRYSEIG GLLSDSTVIN DQDRYRELGK EYAQLEPIVK CFQQFQQNEK 70 80 90 100 110 120 AIESAEEMQQ EKDPELRKLA EEELEQLTLK KEELEDQLKL LLVPKDPNDE RNVFLEIRAG 130 140 150 160 170 180 TGGNEAAIFA GDLFRMYARY AETKGWRVNI VSAHEGEHGG FKEVIARVIG EGVYSQLKFE 190 200 210 220 230 240 SGAHRVQRVP VTESQGRIHT SACTVAIMPE VDEIDQIKIN PAELRIDTFR ASGAGGQHVN 250 260 270 280 290 300 RTDSAIRITH LPTGVVVECQ DERSQHKNKA RAMSLLQSKL LAAERAKQDQ EQAAKRKSLV 310 320 330 340 350 360 GSGDRSERIR TYNFPQGRVT DHRINLTLYQ LDEVIEGDLD PVIGPLIREL QAEQLAELSG E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and linkage analysis of a Coxiella burnetii 2.1 kb NotI fragment." Willems H., Thiele D., Krauss H. Eur. J. Epidemiol. 11:559-561(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile phase I.
[2]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78969 Genomic DNA. Translation: CAA55563.1. AE016828 Genomic DNA. Translation: AAO91454.1.
PIR	A61648.
RefSeq	NP_820940.1. NC_002971.3.
3D structure databases
ProteinModelPortal	P47849.
SMR	P47849. Positions 8-356.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1965.
Proteomic databases
PRIDE	P47849.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91454; AAO91454; CBU_1965.
GeneID	1209878.
KEGG	cbu:CBU_1965.
PATRIC	17932673. VBICoxBur82552_1953.
Phylogenomic databases
eggNOG	COG0216.
HOGENOM	HOG000074815.
KO	K02835.
OMA	EMPEINP.
ProtClustDB	PRK00591.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1939-MONOMER.
Family and domain databases
Gene3D	3.30.160.20. 1 hit.
HAMAP	MF_00093. Rel_fac_1.
InterPro	IPR014720. dsRNA-bd-like_dom. IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. IPR004373. PrfA. [Graphical view]
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00019. prfA. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF1_COXBU

Accession

Primary (citable) accession number: P47849

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 9, 2003
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program