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Protein

Transcription initiation factor TFIID subunit 4

Gene

Taf4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May function as a coactivator by serving as a site of protein-protein contact between activators like Sp1 (or btd) and TFIID complex.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • DNA binding transcription factor activity Source: InterPro
  • protein heterodimerization activity Source: InterPro
  • TFIIA-class transcription factor binding Source: FlyBase
  • transcription coactivator activity Source: GO_Central
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • regulation of transcription, DNA-templated Source: UniProtKB
  • RNA polymerase II transcriptional preinitiation complex assembly Source: GOC
  • transcription by RNA polymerase II Source: UniProtKB
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-674695 RNA Polymerase II Pre-transcription Events
R-DME-73776 RNA Polymerase II Promoter Escape
R-DME-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-DME-75953 RNA Polymerase II Transcription Initiation
R-DME-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 4
Alternative name(s):
110 kDa TBP-associated factor
Transcription initiation factor TFIID 110 kDa subunit
Short name:
TAFII-110
Short name:
p110
Gene namesi
Name:Taf4
Synonyms:TAF110
ORF Names:CG5444
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0010280 Taf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188701 – 921Transcription initiation factor TFIID subunit 4Add BLAST921

Proteomic databases

PaxDbiP47825
PRIDEiP47825

Expressioni

Gene expression databases

BgeeiFBgn0010280
ExpressionAtlasiP47825 differential
GenevisibleiP47825 DM

Interactioni

Subunit structurei

Belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (TAFs). Interacts with TFIIA-L when in complex with Tbp. Interacts with Taf1, Taf5, Taf11 and Taf12.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: InterPro
  • TFIIA-class transcription factor binding Source: FlyBase
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi65074, 32 interactors
DIPiDIP-237N
IntActiP47825, 24 interactors
MINTiP47825
STRINGi7227.FBpp0113081

Structurei

3D structure databases

ProteinModelPortaliP47825
SMRiP47825
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini290 – 386TAFHPROSITE-ProRule annotationAdd BLAST97

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi66 – 82Poly-GlnAdd BLAST17
Compositional biasi108 – 111Poly-Gln4
Compositional biasi259 – 265Poly-Gln7

Sequence similaritiesi

Belongs to the TAF4 family.Curated

Phylogenomic databases

eggNOGiKOG2341 Eukaryota
ENOG410XQS6 LUCA
GeneTreeiENSGT00390000011620
InParanoidiP47825
KOiK03129
OrthoDBiEOG091G03VS
PhylomeDBiP47825

Family and domain databases

CDDicd08045 TAF4, 1 hit
Gene3Di1.10.20.10, 1 hit
1.20.120.1110, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007900 TAF4
IPR037249 TAFH/NHR1_dom_sf
IPR003894 TAFH_NHR1
PfamiView protein in Pfam
PF05236 TAF4, 1 hit
PF07531 TAFH, 1 hit
SMARTiView protein in SMART
SM00549 TAFH, 1 hit
SUPFAMiSSF158553 SSF158553, 1 hit
SSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS51119 TAFH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P47825-1) [UniParc]FASTAAdd to basket
Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTSQTAAGN RITFTSQPLP NGTISIAGNP GAVISTAQLP NTTTIKTIQA
60 70 80 90 100
GIGGQHQGLQ QVHHVQQQQQ SQQQQQQQQQ TQSAGQPLLN SMLPAGVVVG
110 120 130 140 150
MRQQAPSQQQ QKNVPTNPLS RVVINSHMAG VRPQSPSITL STLNTGQTPA
160 170 180 190 200
LLVKTDNGFQ LLRVGTTTGP PTVTQTITNT SNNSNTTSTT NHPTTTQIRL
210 220 230 240 250
QTVPAAASMT NTTATSNIIV NSVASSGYAN SSQPPHLTQL NAQAPQLPQI
260 270 280 290 300
TQIQTIPAQQ SQQQQVNNVS SAGGTATAVS STTAATTTQQ GNTKEKCRKF
310 320 330 340 350
LANLIELSTR EPKPVEKNVR TLIQELVNAN VEPEEFCDRL ERLLNASPQP
360 370 380 390 400
CLIGFLKKSL PLLRQALYTK ELVIEGIKPP PQHVLGLAGL SQQLPKIQAQ
410 420 430 440 450
IRPIGPSQTT TIGQTQVRMI TPNALGTPRP TIGHTTISKQ PPNIRLPTAP
460 470 480 490 500
RLVNTGGIRT QIPSLQVPGQ ANIVQIRGPQ HAQLQRTGSV QIRATTRPPN
510 520 530 540 550
SVPTANKLTA VKVGQTQIKA ITPSLHPPSL AAISGGPPPT PTLSVLSTLN
560 570 580 590 600
SASTTTLPIP SLPTVHLPPE ALRAREQMQN SLNHNSNHFD AKLVEIKAPS
610 620 630 640 650
LHPPHMERIN ASLTPIGAKT MARPPPAINK AIGKKKRDAM EMDAKLNTSS
660 670 680 690 700
GGAASAANSF FQQSSMSSMY GDDDINDVAA MGGVNLAEES QRILGCTENI
710 720 730 740 750
GTQIRSCKDE VFLNLPSLQA RIRAITSEAG LDEPSQDVAV LISHACQERL
760 770 780 790 800
KNIVEKLAVI AEHRIDVIKL DPRYEPAKDV RGQIKFLEEL DKAEQKRHEE
810 820 830 840 850
LEREMLLRAA KSRSRVEDPE QAKMKARAKE MQRAEMEELR QRDANLTALQ
860 870 880 890 900
AIGPRKKLKL DGETVSSGAG SSGGGVLSSS GSAPTTLRPR IKRVNLRDML
910 920
FYMEQEREFC RSSMLFKTYL K
Length:921
Mass (Da):99,338
Last modified:February 1, 1996 - v1
Checksum:i27E6852859872767
GO
Isoform C (identifier: P47825-2) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     138-207: Missing.

Show »
Length:851
Mass (Da):92,061
Checksum:i9BEC6553E724D4A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114V → M in AAL39952 (PubMed:12537569).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004441138 – 207Missing in isoform C. 2 PublicationsAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06861 mRNA No translation available.
S63550 mRNA Translation: AAB27433.1
AE014296 Genomic DNA Translation: AAF49535.1
AE014296 Genomic DNA Translation: AAO41248.1
AY069807 mRNA Translation: AAL39952.1
BT025195 mRNA Translation: ABF17886.2
PIRiA48184
RefSeqiNP_001163453.1, NM_001169982.2 [P47825-1]
NP_001287077.1, NM_001300148.1 [P47825-1]
NP_524102.1, NM_079378.4 [P47825-1]
NP_730109.1, NM_168651.3 [P47825-1]
NP_788511.1, NM_176333.3 [P47825-2]
NP_996101.1, NM_206379.3 [P47825-2]
UniGeneiDm.7812

Genome annotation databases

EnsemblMetazoaiFBtr0075448; FBpp0075205; FBgn0010280 [P47825-1]
FBtr0075449; FBpp0075206; FBgn0010280 [P47825-1]
FBtr0075450; FBpp0075207; FBgn0010280 [P47825-2]
FBtr0075451; FBpp0089259; FBgn0010280 [P47825-2]
FBtr0301911; FBpp0291125; FBgn0010280 [P47825-1]
FBtr0345951; FBpp0311865; FBgn0010280 [P47825-1]
GeneIDi39765
KEGGidme:Dmel_CG5444

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTAF4_DROME
AccessioniPrimary (citable) accession number: P47825
Secondary accession number(s): P49845
, Q0E8E3, Q1LZ31, Q8T9E0, Q9VUY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health