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Protein

P2X purinoceptor 1

Gene

P2rx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ligand-gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Seems to be linked to apoptosis, by increasing the intracellular concentration of calcium in the presence of ATP, leading to programmed cell death.

GO - Molecular functioni

  • ATP binding Source: RGD
  • drug binding Source: RGD
  • extracellular ATP-gated cation channel activity Source: RGD
  • purinergic nucleotide receptor activity Source: Ensembl
  • zinc ion binding Source: RGD

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  • ceramide biosynthetic process Source: Ensembl
  • insemination Source: Ensembl
  • neuronal action potential Source: RGD
  • platelet activation Source: Ensembl
  • positive regulation of ion transport Source: Ensembl
  • protein heterooligomerization Source: RGD
  • protein homooligomerization Source: RGD
  • regulation of blood pressure Source: RGD
  • regulation of calcium ion transport Source: Ensembl
  • regulation of vascular smooth muscle contraction Source: Ensembl
  • response to ATP Source: Ensembl
  • serotonin secretion by platelet Source: Ensembl
  • synaptic transmission, glutamatergic Source: RGD
  • vasoconstriction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Apoptosis, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-139853. Elevation of cytosolic Ca2+ levels.
R-RNO-418346. Platelet homeostasis.

Protein family/group databases

TCDBi1.A.7.1.1. the atp-gated p2x receptor cation channel (p2x receptor) family.

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 1
Short name:
P2X1
Alternative name(s):
ATP receptor
Purinergic receptor
RP-2 protein
Gene namesi
Name:P2rx1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3240. P2rx1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence analysisAdd
BLAST
Transmembranei29 – 5022Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini51 – 338288ExtracellularSequence analysisAdd
BLAST
Transmembranei339 – 35820Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini359 – 39941CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • external side of cell outer membrane Source: Ensembl
  • integral component of plasma membrane Source: InterPro
  • membrane raft Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic membrane Source: Ensembl
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2530.
GuidetoPHARMACOLOGYi478.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399P2X purinoceptor 1PRO_0000161547Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi117 ↔ 165By similarity
Disulfide bondi126 ↔ 149By similarity
Disulfide bondi132 ↔ 159By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence analysis
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence analysis
Disulfide bondi217 ↔ 227By similarity
Disulfide bondi261 ↔ 270By similarity
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence analysis
Modified residuei387 – 3871PhosphoserineBy similarity
Modified residuei388 – 3881PhosphoserineBy similarity
Modified residuei389 – 3891PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP47824.
PRIDEiP47824.

PTM databases

iPTMnetiP47824.
PhosphoSiteiP47824.

Expressioni

Tissue specificityi

High levels in vas deferens and urinary bladder. Lower extent in spinal cord, coeliac ganglion, lung and spleen (probably in the smooth muscle part of both organs).

Developmental stagei

Expressed during apoptosis in thymocytes.

Inductioni

By irradiation and dexamethasone (in thymocytes).

Gene expression databases

ExpressionAtlasiP47824. baseline and differential.
GenevisibleiP47824. RN.

Interactioni

Subunit structurei

Homo- or heteropolymers.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024068.

Chemistry

BindingDBiP47824.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni331 – 3388Pore-forming motifSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi351 – 3544Poly-Leu

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiP47824.
KOiK05215.
OMAiFKYAEDM.
OrthoDBiEOG78PV92.
PhylomeDBiP47824.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003044. P2X1_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF9. PTHR10125:SF9. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01308. P2X1RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRLQDELS AFFFEYDTPR MVLVRNKKVG VIFRLIQLVV LVYVIGWVFV
60 70 80 90 100
YEKGYQTSSD LISSVSVKLK GLAVTQLQGL GPQVWDVADY VFPAHGDSSF
110 120 130 140 150
VVMTNFIVTP QQTQGHCAEN PEGGICQDDS GCTPGKAERK AQGIRTGNCV
160 170 180 190 200
PFNGTVKTCE IFGWCPVEVD DKIPSPALLR EAENFTLFIK NSISFPRFKV
210 220 230 240 250
NRRNLVEEVN GTYMKKCLYH KIQHPLCPVF NLGYVVRESG QDFRSLAEKG
260 270 280 290 300
GVVGITIDWK CDLDWHVRHC KPIYQFHGLY GEKNLSPGFN FRFARHFVQN
310 320 330 340 350
GTNRRHLFKV FGIHFDILVD GKAGKFDIIP TMTTIGSGIG IFGVATVLCD
360 370 380 390
LLLLHILPKR HYYKQKKFKY AEDMGPGEGE HDPVATSSTL GLQENMRTS
Length:399
Mass (Da):44,957
Last modified:February 1, 1996 - v1
Checksum:i04B79E171D78AEC2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti189 – 20113IKNSI…RFKVN → PQLAHGCYPCPPH (PubMed:2072913).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80477 mRNA. Translation: CAA56647.1.
BC061742 mRNA. Translation: AAH61742.1.
M80602 mRNA. Translation: AAA42065.1.
PIRiS50860.
RefSeqiNP_001135839.1. NM_001142367.2.
NP_001272344.1. NM_001285415.1.
NP_037129.1. NM_012997.3.
UniGeneiRn.91176.

Genome annotation databases

EnsembliENSRNOT00000024068; ENSRNOP00000024068; ENSRNOG00000017606.
GeneIDi25505.
KEGGirno:25505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80477 mRNA. Translation: CAA56647.1.
BC061742 mRNA. Translation: AAH61742.1.
M80602 mRNA. Translation: AAA42065.1.
PIRiS50860.
RefSeqiNP_001135839.1. NM_001142367.2.
NP_001272344.1. NM_001285415.1.
NP_037129.1. NM_012997.3.
UniGeneiRn.91176.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024068.

Chemistry

BindingDBiP47824.
ChEMBLiCHEMBL2530.
GuidetoPHARMACOLOGYi478.

Protein family/group databases

TCDBi1.A.7.1.1. the atp-gated p2x receptor cation channel (p2x receptor) family.

PTM databases

iPTMnetiP47824.
PhosphoSiteiP47824.

Proteomic databases

PaxDbiP47824.
PRIDEiP47824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024068; ENSRNOP00000024068; ENSRNOG00000017606.
GeneIDi25505.
KEGGirno:25505.

Organism-specific databases

CTDi5023.
RGDi3240. P2rx1.

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiP47824.
KOiK05215.
OMAiFKYAEDM.
OrthoDBiEOG78PV92.
PhylomeDBiP47824.

Enzyme and pathway databases

ReactomeiR-RNO-139853. Elevation of cytosolic Ca2+ levels.
R-RNO-418346. Platelet homeostasis.

Miscellaneous databases

PROiP47824.

Gene expression databases

ExpressionAtlasiP47824. baseline and differential.
GenevisibleiP47824. RN.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003044. P2X1_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF9. PTHR10125:SF9. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01308. P2X1RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new class of ligand-gated ion channel defined by P2x receptor for extracellular ATP."
    Valera S., Hussy N., Evans R.J., Adami N., North R.A., Surprenant A., Buell G.N.
    Nature 371:516-519(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Vas deferens.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. "Identification of mRNAs associated with programmed cell death in immature thymocytes."
    Owens G.P., Hahn W.E., Cohen J.J.
    Mol. Cell. Biol. 11:4177-4188(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 189-399.
    Strain: Sprague-Dawley.
    Tissue: Thymus.

Entry informationi

Entry nameiP2RX1_RAT
AccessioniPrimary (citable) accession number: P47824
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.