P47824 (P2RX1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2X purinoceptor 1
Short name=P2X1

Alternative name(s):
ATP receptor
Purinergic receptor
RP-2 protein

Gene names

Name:

P2rx1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand-gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Seems to be linked to apoptosis, by increasing the intracellular concentration of calcium in the presence of ATP, leading to programmed cell death.
Subunit structure	Homo- or heteropolymers.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	High levels in vas deferens and urinary bladder. Lower extent in spinal cord, coeliac ganglion, lung and spleen (probably in the smooth muscle part of both organs).
Developmental stage	Expressed during apoptosis in thymocytes.
Induction	By irradiation and dexamethasone (in thymocytes).
Sequence similarities	Belongs to the P2X receptor family.

Ontologies

Keywords
Biological process	Apoptosis Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara ceramide biosynthetic process Inferred from electronic annotation. Source: Compara insemination Inferred from electronic annotation. Source: Compara platelet activation Inferred from electronic annotation. Source: Compara positive regulation of ion transport Inferred from electronic annotation. Source: Compara protein heterooligomerization Inferred from direct assay PubMed 15313628. Source: RGD protein homooligomerization Inferred from direct assay PubMed 15313628PubMed 17287520. Source: RGD regulation of action potential in neuron Inferred from direct assay PubMed 12237343PubMed 16934235. Source: RGD regulation of blood pressure Inferred from mutant phenotype PubMed 14679185. Source: RGD regulation of calcium ion transport Inferred from electronic annotation. Source: Compara regulation of vascular smooth muscle contraction Inferred from electronic annotation. Source: Compara response to ATP Inferred from electronic annotation. Source: Compara serotonin secretion by platelet Inferred from electronic annotation. Source: Compara synaptic transmission, glutamatergic Inferred from mutant phenotype PubMed 16000618. Source: RGD vasoconstriction Inferred from mutant phenotype PubMed 16006561. Source: RGD
Cellular_component	external side of cell outer membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay PubMed 16006561. Source: RGD neuron projection Inferred from direct assay PubMed 16000618. Source: RGD plasma membrane Inferred from direct assay PubMed 11205062PubMed 15313628. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: Compara protein complex Inferred from direct assay PubMed 17287520. Source: RGD
Molecular_function	ATP binding Inferred from direct assay PubMed 12237343PubMed 16934235. Source: RGD drug binding Inferred from direct assay PubMed 12237343. Source: RGD extracellular ATP-gated cation channel activity Inferred from direct assay PubMed 12237343PubMed 16934235. Source: RGD purinergic nucleotide receptor activity Inferred from Biological aspect of Ancestor. Source: RefGenome zinc ion binding Inferred from direct assay PubMed 12237343. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	P2X purinoceptor 1		PRO_0000161547
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 50	22	Helical; Name=1; Potential
	Topological domain	51 – 338	288	Extracellular Potential
	Transmembrane	339 – 358	20	Helical; Name=2; Potential
	Topological domain	359 – 399	41	Cytoplasmic Potential
	Region	331 – 338	8	Pore-forming motif Potential
	Compositional bias	351 – 354	4	Poly-Leu
Amino acid modifications
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	210	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Disulfide bond	117 ↔ 165		By similarity
	Disulfide bond	126 ↔ 149		By similarity
	Disulfide bond	132 ↔ 159		By similarity
	Disulfide bond	217 ↔ 227		By similarity
	Disulfide bond	261 ↔ 270		By similarity
Experimental info
	Sequence conflict	189 – 201	13	IKNSI…RFKVN → PQLAHGCYPCPPH Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P47824 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 04B79E171D78AEC2	FASTA	399	44,957
	10 20 30 40 50 60 MARRLQDELS AFFFEYDTPR MVLVRNKKVG VIFRLIQLVV LVYVIGWVFV YEKGYQTSSD 70 80 90 100 110 120 LISSVSVKLK GLAVTQLQGL GPQVWDVADY VFPAHGDSSF VVMTNFIVTP QQTQGHCAEN 130 140 150 160 170 180 PEGGICQDDS GCTPGKAERK AQGIRTGNCV PFNGTVKTCE IFGWCPVEVD DKIPSPALLR 190 200 210 220 230 240 EAENFTLFIK NSISFPRFKV NRRNLVEEVN GTYMKKCLYH KIQHPLCPVF NLGYVVRESG 250 260 270 280 290 300 QDFRSLAEKG GVVGITIDWK CDLDWHVRHC KPIYQFHGLY GEKNLSPGFN FRFARHFVQN 310 320 330 340 350 360 GTNRRHLFKV FGIHFDILVD GKAGKFDIIP TMTTIGSGIG IFGVATVLCD LLLLHILPKR 370 380 390 HYYKQKKFKY AEDMGPGEGE HDPVATSSTL GLQENMRTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new class of ligand-gated ion channel defined by P2x receptor for extracellular ATP." Valera S., Hussy N., Evans R.J., Adami N., North R.A., Surprenant A., Buell G.N. Nature 371:516-519(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Vas deferens.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	"Identification of mRNAs associated with programmed cell death in immature thymocytes." Owens G.P., Hahn W.E., Cohen J.J. Mol. Cell. Biol. 11:4177-4188(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 189-399. Strain: Sprague-Dawley. Tissue: Thymus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80477 mRNA. Translation: CAA56647.1. BC061742 mRNA. Translation: AAH61742.1. M80602 mRNA. Translation: AAA42065.1.
IPI	IPI00190984.
PIR	S50860.
RefSeq	NP_037129.1. NM_012997.2.
UniGene	Rn.91176.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000024068.
Protein family/group databases
TCDB	1.A.7.1.1. ATP-gated P2X receptor cation channel (P2X Receptor) family.
PTM databases
PhosphoSite	P47824.
Proteomic databases
PRIDE	P47824.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000024068; ENSRNOP00000024068; ENSRNOG00000017606.
GeneID	25505.
KEGG	rno:25505.
Organism-specific databases
CTD	5023.
RGD	3240. P2rx1.
Phylogenomic databases
eggNOG	NOG47843.
GeneTree	ENSGT00390000016028.
HOGENOM	HOG000232042.
HOVERGEN	HBG053086.
InParanoid	P47824.
KO	K05215.
OrthoDB	EOG42FSHT.
Gene expression databases
ArrayExpress	P47824.
Genevestigator	P47824.
GermOnline	ENSRNOG00000017606. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.490.10. 1 hit.
InterPro	IPR003044. P2X1_purnocptor. IPR027309. P2X_extracellular_dom. IPR001429. P2X_purnocptor. [Graphical view]
PANTHER	PTHR10125. PTHR10125. 1 hit. PTHR10125:SF9. PTHR10125:SF9. 1 hit.
Pfam	PF00864. P2X_receptor. 1 hit. [Graphical view]
PRINTS	PR01308. P2X1RECEPTOR. PR01307. P2XRECEPTOR.
TIGRFAMs	TIGR00863. P2X. 1 hit.
PROSITE	PS01212. P2X_RECEPTOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P47824.
ChEMBL	CHEMBL2530.
NextBio	606921.

Entry information

Entry name

P2RX1_RAT

Accession

Primary (citable) accession number: P47824

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program