P47821 (SSN8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase II holoenzyme cyclin-like subunit

Alternative name(s):
Suppressor of RNA polymerase B 11

Gene names

Name:	SSN8
Synonyms:	GIG3, NUT9, SRB11, UME3
Ordered Locus Names:	YNL025C
ORF Names:	N2805

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17
Subunit structure	Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with ASK10. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Subcellular location	Nucleus Ref.17.
Sequence similarities	Belongs to the cyclin family. Cyclin C subfamily. Contains 1 cyclin N-terminal domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator Cyclin Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.14. Source: SGD positive regulation of transcription by galactose Inferred from mutant phenotype Ref.15. Source: SGD positive regulation of transcription from RNA polymerase II promoter during meiosis Inferred from mutant phenotype PubMed 12455972. Source: SGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	DNA-directed RNA polymerase II, holoenzyme Inferred from electronic annotation. Source: InterPro mediator complex Inferred from direct assay PubMed 11383511Ref.9. Source: SGD
Molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity Inferred from mutant phenotype Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	RNA polymerase II holoenzyme cyclin-like subunit		PRO_0000080428
Regions
	Domain	45 – 176	132	Cyclin N-terminal
Amino acid modifications
	Modified residue	266	1	Phosphoserine Ref.18

Sequences

	Sequence		Length	Mass (Da)
	P47821 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2BA16A3374CCF207	FASTA	323	37,791
	10 20 30 40 50 60 MSGSFWTSTQ RHHWQYTKAS LAKERQKLWL LECQLFPQGL NIVMDSKQNG IEQSITKNIP 70 80 90 100 110 120 ITHRDLHYDK DYNLRIYCYF LIMKLGRRLN IRQYALATAH IYLSRFLIKA SVREINLYML 130 140 150 160 170 180 VTTCVYLACK VEECPQYIRT LVSEARTLWP EFIPPDPTKV TEFEFYLLEE LESYLIVHHP 190 200 210 220 230 240 YQSLKQIVQV LKQPPFQITL SSDDLQNCWS LINDSYINDV HLLYPPHIIA VACLFITISI 250 260 270 280 290 300 HGKPTKGSSL ASAASEAIRD PKNSSSPVQI AFNRFMAESL VDLEEVMDTI QEQITLYDHW 310 320 DKYHEQWIKF LLHTLYLRPA SAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A kinase-cyclin pair in the RNA polymerase II holoenzyme." Liao S.-M., Zhang J., Jeffery D.A., Koleske A.J., Thompson C.M., Chao D.M., Viljoen M., van Vuuren H.J.J., Young R.A. Nature 374:193-196(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SSN3. Strain: ATCC 204508 / S288c.
[2]	"UME3, a new C-type cyclin involved in the developmental switch between meiotic and mitotic cell division in S. cerevisiae." Smith J.B., Mallory M.J., Strich R. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast." Kuchin S., Yeghiayan P., Carlson M. Proc. Natl. Acad. Sci. U.S.A. 92:4006-4010(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases." Hengartner C.J., Myer V.E., Liao S.-M., Wilson C.J., Koh S.S., Young R.A. Mol. Cell 2:43-53(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SSN3.
[7]	"The structural and functional organization of the yeast mediator complex." Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J. J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MED1 AND MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[8]	"Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression." Zaman Z., Ansari A.Z., Koh S.S., Young R., Ptashne M. Proc. Natl. Acad. Sci. U.S.A. 98:2550-2554(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SSN3.
[9]	"A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast." Borggrefe T., Davis R., Erdjument-Bromage H., Tempst P., Kornberg R.D. J. Biol. Chem. 277:44202-44207(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SRB8-11 COMPLEX, FUNCTION OF THE SRB8-11 COMPLEX.
[10]	"Ask10p mediates the oxidative stress-induced destruction of the Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p." Cohen T.J., Lee K., Rutkowski L.H., Strich R. Eukaryot. Cell 2:962-970(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ASK10.
[11]	"Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12." Nelson C., Goto S., Lund K., Hung W., Sadowski I. Nature 421:187-190(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"A high resolution protein interaction map of the yeast Mediator complex." Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M. Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[13]	"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts." Nair D., Kim Y., Myers L.C. J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[14]	"Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets." van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P. Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription." Larschan E., Winston F. Mol. Cell. Biol. 25:114-123(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE SRB8-11 COMPLEX.
[16]	"Transcriptional activating regions target attached substrates to a cyclin-dependent kinase." Ansari A.Z., Ogirala A., Ptashne M. Proc. Natl. Acad. Sci. U.S.A. 102:2346-2349(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[17]	"Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA." Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P. Mol. Cell 22:179-192(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[18]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20221 Genomic DNA. Translation: AAA69820.1. U16248 Genomic DNA. Translation: AAA64270.1. U20635 Genomic DNA. Translation: AAA85714.1. Z71301 Genomic DNA. Translation: CAA95887.1. BK006947 Genomic DNA. Translation: DAA10519.1.
PIR	S59373.
RefSeq	NP_014373.3. NM_001182864.3.
3D structure databases
ProteinModelPortal	P47821.
SMR	P47821. Positions 1-306.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2007N.
IntAct	P47821. 28 interactions.
MINT	MINT-385997.
STRING	4932.YNL025C.
Proteomic databases
PaxDb	P47821.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL025C; YNL025C; YNL025C.
GeneID	855706.
KEGG	sce:YNL019C. sce:YNL025C.
Organism-specific databases
CYGD	YNL025c.
SGD	S000004970. SSN8.
Phylogenomic databases
eggNOG	COG5333.
GeneTree	ENSGT00690000101911.
HOGENOM	HOG000217621.
KO	K15161.
OMA	KVEECPQ.
OrthoDB	EOG4BZRBH.
Gene expression databases
Genevestigator	P47821.
GermOnline	YNL025C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR013763. Cyclin-like. IPR015429. Cyclin_C/H/T/L. IPR006671. Cyclin_N. IPR023598. CyclinC. [Graphical view]
PANTHER	PTHR10026. PTHR10026. 1 hit.
Pfam	PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF028758. PIRSF028758. 1 hit.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	980046.

Entry information

Entry name

SSN8_YEAST

Accession

Primary (citable) accession number: P47821
Secondary accession number(s): D6W1F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program