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P47821

- SSN8_YEAST

UniProt

P47821 - SSN8_YEAST

Protein

RNA polymerase II holoenzyme cyclin-like subunit

Gene

SSN8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions.11 Publications

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of transcription by galactose Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
    5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Cyclin, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33063-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase II holoenzyme cyclin-like subunit
    Alternative name(s):
    Suppressor of RNA polymerase B 11
    Gene namesi
    Name:SSN8
    Synonyms:GIG3, NUT9, SRB11, UME3
    Ordered Locus Names:YNL025C
    ORF Names:N2805
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL025c.
    SGDiS000004970. SSN8.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. DNA-directed RNA polymerase II, holoenzyme Source: InterPro
    2. mediator complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323RNA polymerase II holoenzyme cyclin-like subunitPRO_0000080428Add
    BLAST

    Proteomic databases

    MaxQBiP47821.
    PaxDbiP47821.

    Expressioni

    Gene expression databases

    GenevestigatoriP47821.

    Interactioni

    Subunit structurei

    Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with ASK10.6 Publications

    Protein-protein interaction databases

    BioGridi35801. 230 interactions.
    DIPiDIP-2007N.
    IntActiP47821. 33 interactions.
    MINTiMINT-385997.
    STRINGi4932.YNL025C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47821.
    SMRiP47821. Positions 1-306.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 176132Cyclin N-terminalAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cyclin family. Cyclin C subfamily.Curated
    Contains 1 cyclin N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG5333.
    GeneTreeiENSGT00690000101911.
    HOGENOMiHOG000217621.
    KOiK15161.
    OMAiQHIRLIV.
    OrthoDBiEOG744TM6.

    Family and domain databases

    Gene3Di1.10.472.10. 2 hits.
    InterProiIPR013763. Cyclin-like.
    IPR015429. Cyclin_C/H/T/L.
    IPR006671. Cyclin_N.
    IPR023598. CyclinC.
    [Graphical view]
    PANTHERiPTHR10026. PTHR10026. 1 hit.
    PfamiPF00134. Cyclin_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF028758. PIRSF028758. 1 hit.
    SMARTiSM00385. CYCLIN. 2 hits.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P47821-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGSFWTSTQ RHHWQYTKAS LAKERQKLWL LECQLFPQGL NIVMDSKQNG    50
    IEQSITKNIP ITHRDLHYDK DYNLRIYCYF LIMKLGRRLN IRQYALATAH 100
    IYLSRFLIKA SVREINLYML VTTCVYLACK VEECPQYIRT LVSEARTLWP 150
    EFIPPDPTKV TEFEFYLLEE LESYLIVHHP YQSLKQIVQV LKQPPFQITL 200
    SSDDLQNCWS LINDSYINDV HLLYPPHIIA VACLFITISI HGKPTKGSSL 250
    ASAASEAIRD PKNSSSPVQI AFNRFMAESL VDLEEVMDTI QEQITLYDHW 300
    DKYHEQWIKF LLHTLYLRPA SAI 323
    Length:323
    Mass (Da):37,791
    Last modified:February 1, 1996 - v1
    Checksum:i2BA16A3374CCF207
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20221 Genomic DNA. Translation: AAA69820.1.
    U16248 Genomic DNA. Translation: AAA64270.1.
    U20635 Genomic DNA. Translation: AAA85714.1.
    Z71301 Genomic DNA. Translation: CAA95887.1.
    BK006947 Genomic DNA. Translation: DAA10519.1.
    PIRiS59373.
    RefSeqiNP_014373.3. NM_001182864.3.

    Genome annotation databases

    EnsemblFungiiYNL025C; YNL025C; YNL025C.
    GeneIDi855706.
    KEGGisce:YNL025C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20221 Genomic DNA. Translation: AAA69820.1 .
    U16248 Genomic DNA. Translation: AAA64270.1 .
    U20635 Genomic DNA. Translation: AAA85714.1 .
    Z71301 Genomic DNA. Translation: CAA95887.1 .
    BK006947 Genomic DNA. Translation: DAA10519.1 .
    PIRi S59373.
    RefSeqi NP_014373.3. NM_001182864.3.

    3D structure databases

    ProteinModelPortali P47821.
    SMRi P47821. Positions 1-306.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35801. 230 interactions.
    DIPi DIP-2007N.
    IntActi P47821. 33 interactions.
    MINTi MINT-385997.
    STRINGi 4932.YNL025C.

    Proteomic databases

    MaxQBi P47821.
    PaxDbi P47821.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL025C ; YNL025C ; YNL025C .
    GeneIDi 855706.
    KEGGi sce:YNL025C.

    Organism-specific databases

    CYGDi YNL025c.
    SGDi S000004970. SSN8.

    Phylogenomic databases

    eggNOGi COG5333.
    GeneTreei ENSGT00690000101911.
    HOGENOMi HOG000217621.
    KOi K15161.
    OMAi QHIRLIV.
    OrthoDBi EOG744TM6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33063-MONOMER.

    Miscellaneous databases

    NextBioi 980046.
    PROi P47821.

    Gene expression databases

    Genevestigatori P47821.

    Family and domain databases

    Gene3Di 1.10.472.10. 2 hits.
    InterProi IPR013763. Cyclin-like.
    IPR015429. Cyclin_C/H/T/L.
    IPR006671. Cyclin_N.
    IPR023598. CyclinC.
    [Graphical view ]
    PANTHERi PTHR10026. PTHR10026. 1 hit.
    Pfami PF00134. Cyclin_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF028758. PIRSF028758. 1 hit.
    SMARTi SM00385. CYCLIN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SSN3.
      Strain: ATCC 204508 / S288c.
    2. "UME3, a new C-type cyclin involved in the developmental switch between meiotic and mitotic cell division in S. cerevisiae."
      Smith J.B., Mallory M.J., Strich R.
      Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast."
      Kuchin S., Yeghiayan P., Carlson M.
      Proc. Natl. Acad. Sci. U.S.A. 92:4006-4010(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases."
      Hengartner C.J., Myer V.E., Liao S.-M., Wilson C.J., Koh S.S., Young R.A.
      Mol. Cell 2:43-53(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SSN3.
    7. "The structural and functional organization of the yeast mediator complex."
      Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
      J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED1 AND MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    8. "Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression."
      Zaman Z., Ansari A.Z., Koh S.S., Young R., Ptashne M.
      Proc. Natl. Acad. Sci. U.S.A. 98:2550-2554(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SSN3.
    9. "A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast."
      Borggrefe T., Davis R., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      J. Biol. Chem. 277:44202-44207(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SRB8-11 COMPLEX, FUNCTION OF THE SRB8-11 COMPLEX.
    10. "Ask10p mediates the oxidative stress-induced destruction of the Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p."
      Cohen T.J., Lee K., Rutkowski L.H., Strich R.
      Eukaryot. Cell 2:962-970(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ASK10.
    11. "Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12."
      Nelson C., Goto S., Lund K., Hung W., Sadowski I.
      Nature 421:187-190(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
    13. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
      Nair D., Kim Y., Myers L.C.
      J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
    14. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
      van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
      Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription."
      Larschan E., Winston F.
      Mol. Cell. Biol. 25:114-123(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE SRB8-11 COMPLEX.
    16. "Transcriptional activating regions target attached substrates to a cyclin-dependent kinase."
      Ansari A.Z., Ogirala A., Ptashne M.
      Proc. Natl. Acad. Sci. U.S.A. 102:2346-2349(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    17. "Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA."
      Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P.
      Mol. Cell 22:179-192(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSSN8_YEAST
    AccessioniPrimary (citable) accession number: P47821
    Secondary accession number(s): D6W1F3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3