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P47821

- SSN8_YEAST

UniProt

P47821 - SSN8_YEAST

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Protein

RNA polymerase II holoenzyme cyclin-like subunit

Gene
SSN8, GIG3, NUT9, SRB11, UME3, YNL025C, N2805
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions.11 Publications

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  2. positive regulation of transcription by galactose Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter during meiosis Source: SGD
  5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: InterPro
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Cyclin, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33063-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II holoenzyme cyclin-like subunit
Alternative name(s):
Suppressor of RNA polymerase B 11
Gene namesi
Name:SSN8
Synonyms:GIG3, NUT9, SRB11, UME3
Ordered Locus Names:YNL025C
ORF Names:N2805
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL025c.
SGDiS000004970. SSN8.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. DNA-directed RNA polymerase II, holoenzyme Source: InterPro
  2. mediator complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323RNA polymerase II holoenzyme cyclin-like subunitPRO_0000080428Add
BLAST

Proteomic databases

MaxQBiP47821.
PaxDbiP47821.

Expressioni

Gene expression databases

GenevestigatoriP47821.

Interactioni

Subunit structurei

Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with ASK10.6 Publications

Protein-protein interaction databases

BioGridi35801. 230 interactions.
DIPiDIP-2007N.
IntActiP47821. 33 interactions.
MINTiMINT-385997.
STRINGi4932.YNL025C.

Structurei

3D structure databases

ProteinModelPortaliP47821.
SMRiP47821. Positions 1-306.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 176132Cyclin N-terminalAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5333.
GeneTreeiENSGT00690000101911.
HOGENOMiHOG000217621.
KOiK15161.
OMAiQHIRLIV.
OrthoDBiEOG744TM6.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
IPR023598. CyclinC.
[Graphical view]
PANTHERiPTHR10026. PTHR10026. 1 hit.
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF028758. PIRSF028758. 1 hit.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 3 hits.

Sequencei

Sequence statusi: Complete.

P47821-1 [UniParc]FASTAAdd to Basket

« Hide

MSGSFWTSTQ RHHWQYTKAS LAKERQKLWL LECQLFPQGL NIVMDSKQNG    50
IEQSITKNIP ITHRDLHYDK DYNLRIYCYF LIMKLGRRLN IRQYALATAH 100
IYLSRFLIKA SVREINLYML VTTCVYLACK VEECPQYIRT LVSEARTLWP 150
EFIPPDPTKV TEFEFYLLEE LESYLIVHHP YQSLKQIVQV LKQPPFQITL 200
SSDDLQNCWS LINDSYINDV HLLYPPHIIA VACLFITISI HGKPTKGSSL 250
ASAASEAIRD PKNSSSPVQI AFNRFMAESL VDLEEVMDTI QEQITLYDHW 300
DKYHEQWIKF LLHTLYLRPA SAI 323
Length:323
Mass (Da):37,791
Last modified:February 1, 1996 - v1
Checksum:i2BA16A3374CCF207
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20221 Genomic DNA. Translation: AAA69820.1.
U16248 Genomic DNA. Translation: AAA64270.1.
U20635 Genomic DNA. Translation: AAA85714.1.
Z71301 Genomic DNA. Translation: CAA95887.1.
BK006947 Genomic DNA. Translation: DAA10519.1.
PIRiS59373.
RefSeqiNP_014373.3. NM_001182864.3.

Genome annotation databases

EnsemblFungiiYNL025C; YNL025C; YNL025C.
GeneIDi855706.
KEGGisce:YNL025C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20221 Genomic DNA. Translation: AAA69820.1 .
U16248 Genomic DNA. Translation: AAA64270.1 .
U20635 Genomic DNA. Translation: AAA85714.1 .
Z71301 Genomic DNA. Translation: CAA95887.1 .
BK006947 Genomic DNA. Translation: DAA10519.1 .
PIRi S59373.
RefSeqi NP_014373.3. NM_001182864.3.

3D structure databases

ProteinModelPortali P47821.
SMRi P47821. Positions 1-306.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35801. 230 interactions.
DIPi DIP-2007N.
IntActi P47821. 33 interactions.
MINTi MINT-385997.
STRINGi 4932.YNL025C.

Proteomic databases

MaxQBi P47821.
PaxDbi P47821.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL025C ; YNL025C ; YNL025C .
GeneIDi 855706.
KEGGi sce:YNL025C.

Organism-specific databases

CYGDi YNL025c.
SGDi S000004970. SSN8.

Phylogenomic databases

eggNOGi COG5333.
GeneTreei ENSGT00690000101911.
HOGENOMi HOG000217621.
KOi K15161.
OMAi QHIRLIV.
OrthoDBi EOG744TM6.

Enzyme and pathway databases

BioCyci YEAST:G3O-33063-MONOMER.

Miscellaneous databases

NextBioi 980046.
PROi P47821.

Gene expression databases

Genevestigatori P47821.

Family and domain databases

Gene3Di 1.10.472.10. 2 hits.
InterProi IPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
IPR023598. CyclinC.
[Graphical view ]
PANTHERi PTHR10026. PTHR10026. 1 hit.
Pfami PF00134. Cyclin_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF028758. PIRSF028758. 1 hit.
SMARTi SM00385. CYCLIN. 2 hits.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SSN3.
    Strain: ATCC 204508 / S288c.
  2. "UME3, a new C-type cyclin involved in the developmental switch between meiotic and mitotic cell division in S. cerevisiae."
    Smith J.B., Mallory M.J., Strich R.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast."
    Kuchin S., Yeghiayan P., Carlson M.
    Proc. Natl. Acad. Sci. U.S.A. 92:4006-4010(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases."
    Hengartner C.J., Myer V.E., Liao S.-M., Wilson C.J., Koh S.S., Young R.A.
    Mol. Cell 2:43-53(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SSN3.
  7. "The structural and functional organization of the yeast mediator complex."
    Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
    J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED1 AND MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  8. "Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression."
    Zaman Z., Ansari A.Z., Koh S.S., Young R., Ptashne M.
    Proc. Natl. Acad. Sci. U.S.A. 98:2550-2554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SSN3.
  9. "A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast."
    Borggrefe T., Davis R., Erdjument-Bromage H., Tempst P., Kornberg R.D.
    J. Biol. Chem. 277:44202-44207(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SRB8-11 COMPLEX, FUNCTION OF THE SRB8-11 COMPLEX.
  10. "Ask10p mediates the oxidative stress-induced destruction of the Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p."
    Cohen T.J., Lee K., Rutkowski L.H., Strich R.
    Eukaryot. Cell 2:962-970(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ASK10.
  11. "Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12."
    Nelson C., Goto S., Lund K., Hung W., Sadowski I.
    Nature 421:187-190(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
  13. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
    Nair D., Kim Y., Myers L.C.
    J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
  14. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
    van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
    Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription."
    Larschan E., Winston F.
    Mol. Cell. Biol. 25:114-123(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE SRB8-11 COMPLEX.
  16. "Transcriptional activating regions target attached substrates to a cyclin-dependent kinase."
    Ansari A.Z., Ogirala A., Ptashne M.
    Proc. Natl. Acad. Sci. U.S.A. 102:2346-2349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA."
    Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P.
    Mol. Cell 22:179-192(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSSN8_YEAST
AccessioniPrimary (citable) accession number: P47821
Secondary accession number(s): D6W1F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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