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Protein

RNA polymerase II holoenzyme cyclin-like subunit

Gene

SSN8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions.11 Publications

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD
  • RNA polymerase II core promoter sequence-specific DNA binding Source: SGD

GO - Biological processi

Keywordsi

Molecular functionActivator, Cyclin, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33063-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II holoenzyme cyclin-like subunit
Alternative name(s):
Suppressor of RNA polymerase B 11
Gene namesi
Name:SSN8
Synonyms:GIG3, NUT9, SRB11, UME3
Ordered Locus Names:YNL025C
ORF Names:N2805
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL025C.
SGDiS000004970. SSN8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000804281 – 323RNA polymerase II holoenzyme cyclin-like subunitAdd BLAST323

Proteomic databases

MaxQBiP47821.
PRIDEiP47821.

PTM databases

iPTMnetiP47821.

Interactioni

Subunit structurei

Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with ASK10.6 Publications

Protein-protein interaction databases

BioGridi35801. 305 interactors.
DIPiDIP-2007N.
IntActiP47821. 35 interactors.
MINTiMINT-385997.
STRINGi4932.YNL025C.

Structurei

3D structure databases

ProteinModelPortaliP47821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 176Cyclin N-terminalAdd BLAST132

Sequence similaritiesi

Belongs to the cyclin family. Cyclin C subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119191.
HOGENOMiHOG000217621.
InParanoidiP47821.
KOiK15161.
OMAiSSHHQQW.
OrthoDBiEOG092C2LUP.

Family and domain databases

CDDicd00043. CYCLIN. 1 hit.
Gene3Di1.10.472.10. 2 hits.
InterProiView protein in InterPro
IPR013763. Cyclin-like.
IPR036915. Cyclin-like_sf.
IPR006671. Cyclin_N.
IPR028367. CyclinC/Ssn8.
PANTHERiPTHR10026:SF7. PTHR10026:SF7. 1 hit.
PfamiView protein in Pfam
PF00134. Cyclin_N. 1 hit.
SMARTiView protein in SMART
SM00385. CYCLIN. 2 hits.
SUPFAMiSSF47954. SSF47954. 3 hits.

Sequencei

Sequence statusi: Complete.

P47821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSFWTSTQ RHHWQYTKAS LAKERQKLWL LECQLFPQGL NIVMDSKQNG
60 70 80 90 100
IEQSITKNIP ITHRDLHYDK DYNLRIYCYF LIMKLGRRLN IRQYALATAH
110 120 130 140 150
IYLSRFLIKA SVREINLYML VTTCVYLACK VEECPQYIRT LVSEARTLWP
160 170 180 190 200
EFIPPDPTKV TEFEFYLLEE LESYLIVHHP YQSLKQIVQV LKQPPFQITL
210 220 230 240 250
SSDDLQNCWS LINDSYINDV HLLYPPHIIA VACLFITISI HGKPTKGSSL
260 270 280 290 300
ASAASEAIRD PKNSSSPVQI AFNRFMAESL VDLEEVMDTI QEQITLYDHW
310 320
DKYHEQWIKF LLHTLYLRPA SAI
Length:323
Mass (Da):37,791
Last modified:February 1, 1996 - v1
Checksum:i2BA16A3374CCF207
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20221 Genomic DNA. Translation: AAA69820.1.
U16248 Genomic DNA. Translation: AAA64270.1.
U20635 Genomic DNA. Translation: AAA85714.1.
Z71301 Genomic DNA. Translation: CAA95887.1.
BK006947 Genomic DNA. Translation: DAA10519.1.
PIRiS59373.
RefSeqiNP_014373.3. NM_001182864.3.

Genome annotation databases

EnsemblFungiiYNL025C; YNL025C; YNL025C.
GeneIDi855706.
KEGGisce:YNL025C.

Similar proteinsi

Entry informationi

Entry nameiSSN8_YEAST
AccessioniPrimary (citable) accession number: P47821
Secondary accession number(s): D6W1F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 22, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names