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P47821 (SSN8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase II holoenzyme cyclin-like subunit
Alternative name(s):
Suppressor of RNA polymerase B 11
Gene names
Name:SSN8
Synonyms:GIG3, NUT9, SRB11, UME3
Ordered Locus Names:YNL025C
ORF Names:N2805
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17

Subunit structure

Component of the SRB8-11 complex which consists of SRB8, SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair also associate with the RNA polymerase II holoenzyme. Interacts with ASK10. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Nucleus Ref.17.

Sequence similarities

Belongs to the cyclin family. Cyclin C subfamily.

Contains 1 cyclin N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323RNA polymerase II holoenzyme cyclin-like subunit
PRO_0000080428

Regions

Domain45 – 176132Cyclin N-terminal

Sequences

Sequence LengthMass (Da)Tools
P47821 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 2BA16A3374CCF207

FASTA32337,791
        10         20         30         40         50         60 
MSGSFWTSTQ RHHWQYTKAS LAKERQKLWL LECQLFPQGL NIVMDSKQNG IEQSITKNIP 

        70         80         90        100        110        120 
ITHRDLHYDK DYNLRIYCYF LIMKLGRRLN IRQYALATAH IYLSRFLIKA SVREINLYML 

       130        140        150        160        170        180 
VTTCVYLACK VEECPQYIRT LVSEARTLWP EFIPPDPTKV TEFEFYLLEE LESYLIVHHP 

       190        200        210        220        230        240 
YQSLKQIVQV LKQPPFQITL SSDDLQNCWS LINDSYINDV HLLYPPHIIA VACLFITISI 

       250        260        270        280        290        300 
HGKPTKGSSL ASAASEAIRD PKNSSSPVQI AFNRFMAESL VDLEEVMDTI QEQITLYDHW 

       310        320 
DKYHEQWIKF LLHTLYLRPA SAI 

« Hide

References

« Hide 'large scale' references
[1]"A kinase-cyclin pair in the RNA polymerase II holoenzyme."
Liao S.-M., Zhang J., Jeffery D.A., Koleske A.J., Thompson C.M., Chao D.M., Viljoen M., van Vuuren H.J.J., Young R.A.
Nature 374:193-196(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SSN3.
Strain: ATCC 204508 / S288c.
[2]"UME3, a new C-type cyclin involved in the developmental switch between meiotic and mitotic cell division in S. cerevisiae."
Smith J.B., Mallory M.J., Strich R.
Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast."
Kuchin S., Yeghiayan P., Carlson M.
Proc. Natl. Acad. Sci. U.S.A. 92:4006-4010(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases."
Hengartner C.J., Myer V.E., Liao S.-M., Wilson C.J., Koh S.S., Young R.A.
Mol. Cell 2:43-53(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SSN3.
[7]"The structural and functional organization of the yeast mediator complex."
Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J.
J. Biol. Chem. 276:42003-42010(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MED1 AND MED4, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[8]"Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression."
Zaman Z., Ansari A.Z., Koh S.S., Young R., Ptashne M.
Proc. Natl. Acad. Sci. U.S.A. 98:2550-2554(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SSN3.
[9]"A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast."
Borggrefe T., Davis R., Erdjument-Bromage H., Tempst P., Kornberg R.D.
J. Biol. Chem. 277:44202-44207(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SRB8-11 COMPLEX, FUNCTION OF THE SRB8-11 COMPLEX.
[10]"Ask10p mediates the oxidative stress-induced destruction of the Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p."
Cohen T.J., Lee K., Rutkowski L.H., Strich R.
Eukaryot. Cell 2:962-970(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ASK10.
[11]"Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12."
Nelson C., Goto S., Lund K., Hung W., Sadowski I.
Nature 421:187-190(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"A high resolution protein interaction map of the yeast Mediator complex."
Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M.
Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY OF THE MEDIATOR COMPLEX.
[13]"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."
Nair D., Kim Y., Myers L.C.
J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[14]"Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."
van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P., van Leenen D., Holstege F.C.P.
Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription."
Larschan E., Winston F.
Mol. Cell. Biol. 25:114-123(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE SRB8-11 COMPLEX.
[16]"Transcriptional activating regions target attached substrates to a cyclin-dependent kinase."
Ansari A.Z., Ogirala A., Ptashne M.
Proc. Natl. Acad. Sci. U.S.A. 102:2346-2349(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA."
Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P.
Mol. Cell 22:179-192(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20221 Genomic DNA. Translation: AAA69820.1.
U16248 Genomic DNA. Translation: AAA64270.1.
U20635 Genomic DNA. Translation: AAA85714.1.
Z71301 Genomic DNA. Translation: CAA95887.1.
BK006947 Genomic DNA. Translation: DAA10519.1.
PIRS59373.
RefSeqNP_014373.3. NM_001182864.3.

3D structure databases

ProteinModelPortalP47821.
SMRP47821. Positions 1-306.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35801. 228 interactions.
DIPDIP-2007N.
IntActP47821. 33 interactions.
MINTMINT-385997.
STRING4932.YNL025C.

Proteomic databases

MaxQBP47821.
PaxDbP47821.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL025C; YNL025C; YNL025C.
GeneID855706.
KEGGsce:YNL025C.

Organism-specific databases

CYGDYNL025c.
SGDS000004970. SSN8.

Phylogenomic databases

eggNOGCOG5333.
GeneTreeENSGT00690000101911.
HOGENOMHOG000217621.
KOK15161.
OMAQHIRLIV.
OrthoDBEOG744TM6.

Enzyme and pathway databases

BioCycYEAST:G3O-33063-MONOMER.

Gene expression databases

GenevestigatorP47821.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
InterProIPR013763. Cyclin-like.
IPR015429. Cyclin_C/H/T/L.
IPR006671. Cyclin_N.
IPR023598. CyclinC.
[Graphical view]
PANTHERPTHR10026. PTHR10026. 1 hit.
PfamPF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFPIRSF028758. PIRSF028758. 1 hit.
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 3 hits.
ProtoNetSearch...

Other

NextBio980046.
PROP47821.

Entry information

Entry nameSSN8_YEAST
AccessionPrimary (citable) accession number: P47821
Secondary accession number(s): D6W1F3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families