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P47818 (CCC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein CCC1
Alternative name(s):
Cross-complementer of CSG1 protein 1
Gene names
Name:CCC1
Ordered Locus Names:YLR220W
ORF Names:L8083.6
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a role in both calcium and manganese homeostasis. Involved in the transfer of iron and Mn2+ from the cytosol to the vacuole for storage of these metals. Ref.5 Ref.6 Ref.8

Subcellular location

Golgi apparatus membrane. Vacuole membrane; Multi-pass membrane protein Potential Ref.6 Ref.8 Ref.9.

Induction

Down-regulated under iron starvation by TIS11. Transcriptionally up-regulated by YAP5 in response to increased cytosolic iron. Ref.12 Ref.16

Disruption phenotype

Deletion causes an increase in metal sensitivity at 15 mM manganese, but does not affect invertase glycosylation. Deletion shows increased sensitivity to external iron, which is suppressed by MRS3 or MRS4 overexpression requiring oxygen but not respiration, and exacerbated by ectopic expression of the iron transporter FET4. Deletion also results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth. Deletion together with MRS3 and MRS4 restores cellular and mitochondrial iron homeostasis to near normal level, corrects the MRS3 and MRS4 double deletion phenotype (which shows increased resistance to cobalt but decreased resistance to copper and cadmium), and has near normal levels of aconitase activity. When overexpressed, maintains respiratory function in a YFH1 deletion mutant regardless of extracellular iron concentration, activates the iron-dependent transcription factor AFT1 resulting in an increase in iron uptake, cytosolic iron accumulation and a change in copper metabolism. Overexpression prevents excessive mitochondrial iron accumulation by limiting mitochondrial iron uptake and results in increased expression of the high affinity iron transport system composed of FET3 and FTR1. Overexpression also suppresses the rapamycin-resistant phenotype of PMR1 deletion mutant. Ref.6

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CCC1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ECM4P361561EBI-2048372,EBI-2042717

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Protein CCC1
PRO_0000089394

Regions

Topological domain1 – 9999Cytoplasmic Potential
Transmembrane100 – 12021Helical; Potential
Topological domain121 – 1299Vacuolar Potential
Transmembrane130 – 15021Helical; Potential
Topological domain151 – 23686Cytoplasmic Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 2592Vacuolar Potential
Transmembrane260 – 28021Helical; Potential
Topological domain281 – 30020Cytoplasmic Potential
Transmembrane301 – 32121Helical; Potential
Topological domain3221Vacuolar Potential

Amino acid modifications

Modified residue291Phosphoserine Ref.17
Modified residue531Phosphoserine Ref.18
Modified residue681Phosphoserine Ref.17 Ref.18
Modified residue711Phosphoserine Ref.17 Ref.18
Modified residue831Phosphoserine Ref.15 Ref.17 Ref.18

Sequences

Sequence LengthMass (Da)Tools
P47818 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 374081A49F0C43D1

FASTA32234,250
        10         20         30         40         50         60 
MSIVALKNAV VTLIQKAKGS GGTSELGGSE STPLLRGSNS NSSRHDNLSS SSSDIIYGRN 

        70         80         90        100        110        120 
SAQDLENSPM SVGKDNRNGD NGSDNEKANL GFFQSVDPRV ISDLIIGLSD GLTVPFALTA 

       130        140        150        160        170        180 
GLSSLGDAKL VITGGFAELI SGAISMGLGG YLGAKSESDY YHAEVKKEKR KFYDNSNLIN 

       190        200        210        220        230        240 
REIEDILLEI NPNFSDETIV SFIKDLQRTP ELMVDFIIRY GRGLDEPAEN RELISAVTIG 

       250        260        270        280        290        300 
GGYLLGGLVP LVPYFFVSDV GTGLIYSIIV MVVTLFWFGY VKTKLSMGSG SSTSKKVTEG 

       310        320 
VEMVVVGGVA AGAAWFFVKL LG 

« Hide

References

« Hide 'large scale' references
[1]"Sequence, mapping and disruption of CCC1, a gene that cross-complements the Ca(2+)-sensitive phenotype of csg1 mutants."
Fu D., Beeler T.J., Dunn T.M.
Yeast 10:515-521(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangers."
Pozos T.C., Sekler I., Cyert M.S.
Mol. Cell. Biol. 16:3730-3741(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: S288c / YPH1.
[6]"The role of the Saccharomyces cerevisiae CCC1 gene in the homeostasis of manganese ions."
Lapinskas P.J., Lin S.-J., Culotta V.C.
Mol. Microbiol. 21:519-528(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[7]"CCC1 suppresses mitochondrial damage in the yeast model of Friedreich's ataxia by limiting mitochondrial iron accumulation."
Chen O.S., Kaplan J.
J. Biol. Chem. 275:7626-7632(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: OVEREXPRESSION PHENOTYPE.
[8]"CCC1 is a transporter that mediates vacuolar iron storage in yeast."
Li L., Chen O.S., McVey Ward D., Kaplan J.
J. Biol. Chem. 276:29515-29519(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, CHARACTERIZATION OF DELETION MUTANT.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism."
Li L., Kaplan J.
J. Biol. Chem. 279:33653-33661(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF DELETION MUTANT.
[12]"Coordinated remodeling of cellular metabolism during iron deficiency through targeted mRNA degradation."
Puig S., Askeland E., Thiele D.J.
Cell 120:99-110(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[13]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[14]"Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae."
Devasahayam G., Burke D.J., Sturgill T.W.
Genetics 177:231-238(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: EFFECT OF OVEREXPRESSION.
[15]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[16]"Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast."
Li L., Bagley D., Ward D.M., Kaplan J.
Mol. Cell. Biol. 28:1326-1337(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[17]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-68; SER-71 AND SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; SER-68; SER-71 AND SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L24112 mRNA. Translation: AAA62622.1.
U14913 Genomic DNA. Translation: AAB67452.1.
U19027 Genomic DNA. Translation: AAB67409.1.
AY557952 Genomic DNA. Translation: AAS56278.1.
BK006945 Genomic DNA. Translation: DAA09536.1.
PIRS43453.
RefSeqNP_013321.1. NM_001182107.1.

3D structure databases

ProteinModelPortalP47818.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31487. 39 interactions.
DIPDIP-782N.
MINTMINT-4495435.
STRING4932.YLR220W.

Protein family/group databases

TCDB2.A.89.1.1. the vacuolar iron transporter (vit) family.

Proteomic databases

PaxDbP47818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR220W; YLR220W; YLR220W.
GeneID850917.
KEGGsce:YLR220W.

Organism-specific databases

CYGDYLR220w.
SGDS000004210. CCC1.

Phylogenomic databases

eggNOGCOG1814.
HOGENOMHOG000096548.
OMAFGYGKTC.
OrthoDBEOG7673MS.

Enzyme and pathway databases

BioCycYEAST:G3O-32334-MONOMER.

Gene expression databases

GenevestigatorP47818.

Family and domain databases

InterProIPR008217. DUF125_TM.
[Graphical view]
PfamPF01988. VIT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967332.

Entry information

Entry nameCCC1_YEAST
AccessionPrimary (citable) accession number: P47818
Secondary accession number(s): D6VYM0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families