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P47818

- CCC1_YEAST

UniProt

P47818 - CCC1_YEAST

Protein

Protein CCC1

Gene

CCC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Has a role in both calcium and manganese homeostasis. Involved in the transfer of iron and Mn2+ from the cytosol to the vacuole for storage of these metals.3 Publications

    GO - Molecular functioni

    1. ferrous iron transmembrane transporter activity Source: SGD
    2. manganese ion transmembrane transporter activity Source: SGD

    GO - Biological processi

    1. cellular calcium ion homeostasis Source: SGD
    2. cellular iron ion homeostasis Source: SGD
    3. cellular manganese ion homeostasis Source: SGD
    4. ferrous iron transport Source: SGD
    5. manganese ion transmembrane transport Source: GOC
    6. manganese ion transport Source: SGD

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32334-MONOMER.

    Protein family/group databases

    TCDBi2.A.89.1.1. the vacuolar iron transporter (vit) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein CCC1
    Alternative name(s):
    Cross-complementer of CSG1 protein 1
    Gene namesi
    Name:CCC1
    Ordered Locus Names:YLR220W
    ORF Names:L8083.6
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR220w.
    SGDiS000004210. CCC1.

    Subcellular locationi

    Golgi apparatus membrane 3 Publications. Vacuole membrane Curated; Multi-pass membrane protein Curated

    GO - Cellular componenti

    1. fungal-type vacuole Source: SGD
    2. fungal-type vacuole membrane Source: SGD
    3. Golgi apparatus Source: SGD
    4. Golgi membrane Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Vacuole

    Pathology & Biotechi

    Disruption phenotypei

    Deletion causes an increase in metal sensitivity at 15 mM manganese, but does not affect invertase glycosylation. Deletion shows increased sensitivity to external iron, which is suppressed by MRS3 or MRS4 overexpression requiring oxygen but not respiration, and exacerbated by ectopic expression of the iron transporter FET4. Deletion also results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth. Deletion together with MRS3 and MRS4 restores cellular and mitochondrial iron homeostasis to near normal level, corrects the MRS3 and MRS4 double deletion phenotype (which shows increased resistance to cobalt but decreased resistance to copper and cadmium), and has near normal levels of aconitase activity. When overexpressed, maintains respiratory function in a YFH1 deletion mutant regardless of extracellular iron concentration, activates the iron-dependent transcription factor AFT1 resulting in an increase in iron uptake, cytosolic iron accumulation and a change in copper metabolism. Overexpression prevents excessive mitochondrial iron accumulation by limiting mitochondrial iron uptake and results in increased expression of the high affinity iron transport system composed of FET3 and FTR1. Overexpression also suppresses the rapamycin-resistant phenotype of PMR1 deletion mutant.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322Protein CCC1PRO_0000089394Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei29 – 291Phosphoserine1 Publication
    Modified residuei53 – 531Phosphoserine1 Publication
    Modified residuei68 – 681Phosphoserine2 Publications
    Modified residuei71 – 711Phosphoserine2 Publications
    Modified residuei83 – 831Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47818.
    PaxDbiP47818.

    Expressioni

    Inductioni

    Down-regulated under iron starvation by TIS11. Transcriptionally up-regulated by YAP5 in response to increased cytosolic iron.2 Publications

    Gene expression databases

    GenevestigatoriP47818.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ECM4P361561EBI-2048372,EBI-2042717

    Protein-protein interaction databases

    BioGridi31487. 39 interactions.
    DIPiDIP-782N.
    MINTiMINT-4495435.
    STRINGi4932.YLR220W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47818.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini121 – 1299VacuolarSequence Analysis
    Topological domaini151 – 23686CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini258 – 2592VacuolarSequence Analysis
    Topological domaini281 – 30020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini322 – 3221VacuolarSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei100 – 12021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei301 – 32121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CCC1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1814.
    HOGENOMiHOG000096548.
    OMAiLDFMMRF.
    OrthoDBiEOG7673MS.

    Family and domain databases

    InterProiIPR008217. DUF125_TM.
    [Graphical view]
    PfamiPF01988. VIT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIVALKNAV VTLIQKAKGS GGTSELGGSE STPLLRGSNS NSSRHDNLSS    50
    SSSDIIYGRN SAQDLENSPM SVGKDNRNGD NGSDNEKANL GFFQSVDPRV 100
    ISDLIIGLSD GLTVPFALTA GLSSLGDAKL VITGGFAELI SGAISMGLGG 150
    YLGAKSESDY YHAEVKKEKR KFYDNSNLIN REIEDILLEI NPNFSDETIV 200
    SFIKDLQRTP ELMVDFIIRY GRGLDEPAEN RELISAVTIG GGYLLGGLVP 250
    LVPYFFVSDV GTGLIYSIIV MVVTLFWFGY VKTKLSMGSG SSTSKKVTEG 300
    VEMVVVGGVA AGAAWFFVKL LG 322
    Length:322
    Mass (Da):34,250
    Last modified:February 1, 1996 - v1
    Checksum:i374081A49F0C43D1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24112 mRNA. Translation: AAA62622.1.
    U14913 Genomic DNA. Translation: AAB67452.1.
    U19027 Genomic DNA. Translation: AAB67409.1.
    AY557952 Genomic DNA. Translation: AAS56278.1.
    BK006945 Genomic DNA. Translation: DAA09536.1.
    PIRiS43453.
    RefSeqiNP_013321.1. NM_001182107.1.

    Genome annotation databases

    EnsemblFungiiYLR220W; YLR220W; YLR220W.
    GeneIDi850917.
    KEGGisce:YLR220W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24112 mRNA. Translation: AAA62622.1 .
    U14913 Genomic DNA. Translation: AAB67452.1 .
    U19027 Genomic DNA. Translation: AAB67409.1 .
    AY557952 Genomic DNA. Translation: AAS56278.1 .
    BK006945 Genomic DNA. Translation: DAA09536.1 .
    PIRi S43453.
    RefSeqi NP_013321.1. NM_001182107.1.

    3D structure databases

    ProteinModelPortali P47818.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31487. 39 interactions.
    DIPi DIP-782N.
    MINTi MINT-4495435.
    STRINGi 4932.YLR220W.

    Protein family/group databases

    TCDBi 2.A.89.1.1. the vacuolar iron transporter (vit) family.

    Proteomic databases

    MaxQBi P47818.
    PaxDbi P47818.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR220W ; YLR220W ; YLR220W .
    GeneIDi 850917.
    KEGGi sce:YLR220W.

    Organism-specific databases

    CYGDi YLR220w.
    SGDi S000004210. CCC1.

    Phylogenomic databases

    eggNOGi COG1814.
    HOGENOMi HOG000096548.
    OMAi LDFMMRF.
    OrthoDBi EOG7673MS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32334-MONOMER.

    Miscellaneous databases

    NextBioi 967332.

    Gene expression databases

    Genevestigatori P47818.

    Family and domain databases

    InterProi IPR008217. DUF125_TM.
    [Graphical view ]
    Pfami PF01988. VIT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, mapping and disruption of CCC1, a gene that cross-complements the Ca(2+)-sensitive phenotype of csg1 mutants."
      Fu D., Beeler T.J., Dunn T.M.
      Yeast 10:515-521(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangers."
      Pozos T.C., Sekler I., Cyert M.S.
      Mol. Cell. Biol. 16:3730-3741(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: S288c / YPH1.
    6. "The role of the Saccharomyces cerevisiae CCC1 gene in the homeostasis of manganese ions."
      Lapinskas P.J., Lin S.-J., Culotta V.C.
      Mol. Microbiol. 21:519-528(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    7. "CCC1 suppresses mitochondrial damage in the yeast model of Friedreich's ataxia by limiting mitochondrial iron accumulation."
      Chen O.S., Kaplan J.
      J. Biol. Chem. 275:7626-7632(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: OVEREXPRESSION PHENOTYPE.
    8. "CCC1 is a transporter that mediates vacuolar iron storage in yeast."
      Li L., Chen O.S., McVey Ward D., Kaplan J.
      J. Biol. Chem. 276:29515-29519(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, CHARACTERIZATION OF DELETION MUTANT.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism."
      Li L., Kaplan J.
      J. Biol. Chem. 279:33653-33661(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF DELETION MUTANT.
    12. "Coordinated remodeling of cellular metabolism during iron deficiency through targeted mRNA degradation."
      Puig S., Askeland E., Thiele D.J.
      Cell 120:99-110(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    13. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    14. "Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae."
      Devasahayam G., Burke D.J., Sturgill T.W.
      Genetics 177:231-238(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: EFFECT OF OVEREXPRESSION.
    15. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    16. "Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast."
      Li L., Bagley D., Ward D.M., Kaplan J.
      Mol. Cell. Biol. 28:1326-1337(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-68; SER-71 AND SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; SER-68; SER-71 AND SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCCC1_YEAST
    AccessioniPrimary (citable) accession number: P47818
    Secondary accession number(s): D6VYM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2840 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3