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Protein

Protein CCC1

Gene

CCC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in both calcium and manganese homeostasis. Involved in the transfer of iron and Mn2+ from the cytosol to the vacuole for storage of these metals.3 Publications

GO - Molecular functioni

  1. ferrous iron transmembrane transporter activity Source: SGD
  2. manganese ion transmembrane transporter activity Source: SGD

GO - Biological processi

  1. cellular calcium ion homeostasis Source: SGD
  2. cellular iron ion homeostasis Source: SGD
  3. cellular manganese ion homeostasis Source: SGD
  4. ferrous iron transport Source: SGD
  5. manganese ion transmembrane transport Source: GOC
  6. manganese ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32334-MONOMER.

Protein family/group databases

TCDBi2.A.89.1.1. the vacuolar iron transporter (vit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CCC1
Alternative name(s):
Cross-complementer of CSG1 protein 1
Gene namesi
Name:CCC1
Ordered Locus Names:YLR220W
ORF Names:L8083.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR220w.
SGDiS000004210. CCC1.

Subcellular locationi

Golgi apparatus membrane 3 Publications. Vacuole membrane Curated; Multi-pass membrane protein Curated

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence AnalysisAdd
BLAST
Topological domaini121 – 1299VacuolarSequence Analysis
Transmembranei130 – 15021HelicalSequence AnalysisAdd
BLAST
Topological domaini151 – 23686CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei237 – 25721HelicalSequence AnalysisAdd
BLAST
Topological domaini258 – 2592VacuolarSequence Analysis
Transmembranei260 – 28021HelicalSequence AnalysisAdd
BLAST
Topological domaini281 – 30020CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei301 – 32121HelicalSequence AnalysisAdd
BLAST
Topological domaini322 – 3221VacuolarSequence Analysis

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
  2. fungal-type vacuole membrane Source: SGD
  3. Golgi apparatus Source: SGD
  4. Golgi membrane Source: UniProtKB-SubCell
  5. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Deletion causes an increase in metal sensitivity at 15 mM manganese, but does not affect invertase glycosylation. Deletion shows increased sensitivity to external iron, which is suppressed by MRS3 or MRS4 overexpression requiring oxygen but not respiration, and exacerbated by ectopic expression of the iron transporter FET4. Deletion also results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth. Deletion together with MRS3 and MRS4 restores cellular and mitochondrial iron homeostasis to near normal level, corrects the MRS3 and MRS4 double deletion phenotype (which shows increased resistance to cobalt but decreased resistance to copper and cadmium), and has near normal levels of aconitase activity. When overexpressed, maintains respiratory function in a YFH1 deletion mutant regardless of extracellular iron concentration, activates the iron-dependent transcription factor AFT1 resulting in an increase in iron uptake, cytosolic iron accumulation and a change in copper metabolism. Overexpression prevents excessive mitochondrial iron accumulation by limiting mitochondrial iron uptake and results in increased expression of the high affinity iron transport system composed of FET3 and FTR1. Overexpression also suppresses the rapamycin-resistant phenotype of PMR1 deletion mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Protein CCC1PRO_0000089394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Phosphoserine1 Publication
Modified residuei53 – 531Phosphoserine1 Publication
Modified residuei68 – 681Phosphoserine2 Publications
Modified residuei71 – 711Phosphoserine2 Publications
Modified residuei83 – 831Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47818.
PaxDbiP47818.

Expressioni

Inductioni

Down-regulated under iron starvation by TIS11. Transcriptionally up-regulated by YAP5 in response to increased cytosolic iron.2 Publications

Gene expression databases

GenevestigatoriP47818.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ECM4P361561EBI-2048372,EBI-2042717

Protein-protein interaction databases

BioGridi31487. 40 interactions.
DIPiDIP-782N.
MINTiMINT-4495435.
STRINGi4932.YLR220W.

Structurei

3D structure databases

ProteinModelPortaliP47818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CCC1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1814.
HOGENOMiHOG000096548.
InParanoidiP47818.
OMAiFGYGKTC.
OrthoDBiEOG7673MS.

Family and domain databases

InterProiIPR008217. Ccc1_fam.
[Graphical view]
PfamiPF01988. VIT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47818-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIVALKNAV VTLIQKAKGS GGTSELGGSE STPLLRGSNS NSSRHDNLSS
60 70 80 90 100
SSSDIIYGRN SAQDLENSPM SVGKDNRNGD NGSDNEKANL GFFQSVDPRV
110 120 130 140 150
ISDLIIGLSD GLTVPFALTA GLSSLGDAKL VITGGFAELI SGAISMGLGG
160 170 180 190 200
YLGAKSESDY YHAEVKKEKR KFYDNSNLIN REIEDILLEI NPNFSDETIV
210 220 230 240 250
SFIKDLQRTP ELMVDFIIRY GRGLDEPAEN RELISAVTIG GGYLLGGLVP
260 270 280 290 300
LVPYFFVSDV GTGLIYSIIV MVVTLFWFGY VKTKLSMGSG SSTSKKVTEG
310 320
VEMVVVGGVA AGAAWFFVKL LG
Length:322
Mass (Da):34,250
Last modified:February 1, 1996 - v1
Checksum:i374081A49F0C43D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24112 mRNA. Translation: AAA62622.1.
U14913 Genomic DNA. Translation: AAB67452.1.
U19027 Genomic DNA. Translation: AAB67409.1.
AY557952 Genomic DNA. Translation: AAS56278.1.
BK006945 Genomic DNA. Translation: DAA09536.1.
PIRiS43453.
RefSeqiNP_013321.1. NM_001182107.1.

Genome annotation databases

EnsemblFungiiYLR220W; YLR220W; YLR220W.
GeneIDi850917.
KEGGisce:YLR220W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24112 mRNA. Translation: AAA62622.1.
U14913 Genomic DNA. Translation: AAB67452.1.
U19027 Genomic DNA. Translation: AAB67409.1.
AY557952 Genomic DNA. Translation: AAS56278.1.
BK006945 Genomic DNA. Translation: DAA09536.1.
PIRiS43453.
RefSeqiNP_013321.1. NM_001182107.1.

3D structure databases

ProteinModelPortaliP47818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31487. 40 interactions.
DIPiDIP-782N.
MINTiMINT-4495435.
STRINGi4932.YLR220W.

Protein family/group databases

TCDBi2.A.89.1.1. the vacuolar iron transporter (vit) family.

Proteomic databases

MaxQBiP47818.
PaxDbiP47818.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR220W; YLR220W; YLR220W.
GeneIDi850917.
KEGGisce:YLR220W.

Organism-specific databases

CYGDiYLR220w.
SGDiS000004210. CCC1.

Phylogenomic databases

eggNOGiCOG1814.
HOGENOMiHOG000096548.
InParanoidiP47818.
OMAiFGYGKTC.
OrthoDBiEOG7673MS.

Enzyme and pathway databases

BioCyciYEAST:G3O-32334-MONOMER.

Miscellaneous databases

NextBioi967332.

Gene expression databases

GenevestigatoriP47818.

Family and domain databases

InterProiIPR008217. Ccc1_fam.
[Graphical view]
PfamiPF01988. VIT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence, mapping and disruption of CCC1, a gene that cross-complements the Ca(2+)-sensitive phenotype of csg1 mutants."
    Fu D., Beeler T.J., Dunn T.M.
    Yeast 10:515-521(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangers."
    Pozos T.C., Sekler I., Cyert M.S.
    Mol. Cell. Biol. 16:3730-3741(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: S288c / YPH1.
  6. "The role of the Saccharomyces cerevisiae CCC1 gene in the homeostasis of manganese ions."
    Lapinskas P.J., Lin S.-J., Culotta V.C.
    Mol. Microbiol. 21:519-528(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  7. "CCC1 suppresses mitochondrial damage in the yeast model of Friedreich's ataxia by limiting mitochondrial iron accumulation."
    Chen O.S., Kaplan J.
    J. Biol. Chem. 275:7626-7632(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: OVEREXPRESSION PHENOTYPE.
  8. "CCC1 is a transporter that mediates vacuolar iron storage in yeast."
    Li L., Chen O.S., McVey Ward D., Kaplan J.
    J. Biol. Chem. 276:29515-29519(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, CHARACTERIZATION OF DELETION MUTANT.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism."
    Li L., Kaplan J.
    J. Biol. Chem. 279:33653-33661(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF DELETION MUTANT.
  12. "Coordinated remodeling of cellular metabolism during iron deficiency through targeted mRNA degradation."
    Puig S., Askeland E., Thiele D.J.
    Cell 120:99-110(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  13. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  14. "Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae."
    Devasahayam G., Burke D.J., Sturgill T.W.
    Genetics 177:231-238(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EFFECT OF OVEREXPRESSION.
  15. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  16. "Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast."
    Li L., Bagley D., Ward D.M., Kaplan J.
    Mol. Cell. Biol. 28:1326-1337(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29; SER-68; SER-71 AND SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; SER-68; SER-71 AND SER-83, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCCC1_YEAST
AccessioniPrimary (citable) accession number: P47818
Secondary accession number(s): D6VYM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.