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Protein

Mitogen-activated protein kinase 14

Gene

mapk14

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. mapk14a is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. MPK2 is activated by upstream MAPKK/MAPKKK and stimulates MAPKAP kinase 2 to phosphorylate small heat shock proteins. Does not phosphorylate myelin basic protein or MAPKAP kinase 1.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei151Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14 (EC:2.7.11.24)
Short name:
MAP kinase 14
Short name:
MAPK 14
Alternative name(s):
Mitogen-activated Mitogen-activated protein kinase 2
Short name:
MAP kinase 2
Short name:
MPK2
Gene namesi
Name:mapk14
Synonyms:mpk2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1018624. mapk14.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001862991 – 361Mitogen-activated protein kinase 14Add BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphothreonineBy similarity1
Modified residuei183PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47812.
PRIDEiP47812.

PTM databases

iPTMnetiP47812.

Interactioni

Protein-protein interaction databases

BioGridi98234. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliP47812.
SMRiP47812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 309Protein kinasePROSITE-ProRule annotationAdd BLAST285

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 183TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG014652.
KOiK04441.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PRINTSiPR01773. P38MAPKINASE.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.

Sequencei

Sequence statusi: Complete.

P47812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNQSYVFY RQELNKTLWE VPDRYQNLTP VGSGAYGSVC SSFDTRTALR
60 70 80 90 100
IAVKKLSRPF QSIIHAKRTY RELRLLKHMK HENVIGLLDV FSPAKSFEEF
110 120 130 140 150
NDVYLVTHLM GADLNNIVKC QKLTDDHVQF LIYQILRGLK YIHSAGIIHR
160 170 180 190 200
DLKPSNLAVN EDCELKILDF GLARHTDEEM TGYVATRWYR APEIMLNWMH
210 220 230 240 250
YNQTVDIWSV GCIMAELLTG RTLFPGTDHI DQLKLILRLV GTPEPELLQK
260 270 280 290 300
ISSEAARNYI QSLPYMPKMN FEDVFLGANP QAVDLLEKML VLDTDKRITA
310 320 330 340 350
AEALAHSYFA QYHDPDDEPI AEPYDQSFES RELDIEEWKR LTYEEVTCFV
360
PPPLDSEEME S
Length:361
Mass (Da):41,719
Last modified:February 1, 1996 - v1
Checksum:iF2B2E6C40800A70D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80751 mRNA. Translation: CAA56727.1.
BC056064 mRNA. Translation: AAH56064.1.
PIRiA54805.
RefSeqiNP_001080300.1. NM_001086831.1.
UniGeneiXl.1245.

Genome annotation databases

GeneIDi379992.
KEGGixla:379992.

Similar proteinsi

Entry informationi

Entry nameiMK14_XENLA
AccessioniPrimary (citable) accession number: P47812
Secondary accession number(s): Q5D076
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 22, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families