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UniProtKB - P47811 (MK14_MOUSE)

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Protein

Mitogen-activated protein kinase 14

Gene

Mapk14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Phosphorylates S100A9 at 'Thr-113' (By similarity).By similarity4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+

Enzyme regulationi

Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as bacterial lipopolysaccharide (LPS) and interleukin-1. Activation occurs through dual phosphorylation of Thr-180 and Tyr-182 by either of two dual specificity kinases, MAP2K3/MKK3 or MAP2K6/MKK6, and potentially also MAP2K4/MKK4, as well as by TAB1-mediated autophosphorylation. MAPK14 phosphorylated on both Thr-180 and Tyr-182 is 10-20-fold more active than MAPK14 phosphorylated only on Thr-180, whereas MAPK14 phosphorylated on Tyr-182 alone is inactive. whereas Thr-180 is necessary for catalysis, Tyr-182 may be required for auto-activation and substrate recognition. Phosphorylated at Tyr-323 by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells. This alternative pathway is inhibited by GADD45A. Inhibited by dual specificity phosphatases, such as DUSP1, DUSP10, and DUSP16. Specifically inhibited by the binding of pyridinyl-imidazole compounds, which are cytokine-suppressive anti-inflammatory drugs (CSAID). SB203580 is an inhibitor of MAPK14.3 Publications

Kineticsi

  1. KM=212 µM for ATP (when both Thr-180 and Tyr-182 are phosphorylated)1 Publication
  2. KM=1669 µM for ATP (when only Thr-180 is phosphorylated)1 Publication
  3. KM=656 µM for EGFR peptide as a substrate (when both Thr-180 and Tyr-182 are phosphorylated)1 Publication
  4. KM=2800 µM for EGFR peptide as a substrate (when only Thr-180 is phosphorylated)1 Publication
  5. KM=2.03 µM for ATF2 as a substrate (when both Thr-180 and Tyr-182 are phosphorylated)1 Publication
  6. KM=20.1 µM for ATF2 as a substrate (when only Thr-180 is phosphorylated)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei32Inhibitor2 Publications1
    Binding sitei35Inhibitor2 Publications1
    Binding sitei53ATPPROSITE-ProRule annotation1
    Binding sitei53Inhibitor2 Publications1
    Binding sitei71Inhibitor2 Publications1
    Binding sitei109Inhibitor; via amide nitrogen and carbonyl oxygen2 Publications1
    Binding sitei111Inhibitor; via amide nitrogen2 Publications1
    Active sitei168Proton acceptorPROSITE-ProRule annotation1
    Binding sitei168Inhibitor2 Publications1
    Binding sitei169Inhibitor; via carbonyl oxygen2 Publications1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi30 – 38ATPPROSITE-ProRule annotation9

    GO - Molecular functioni

    • ATP binding Source: MGI
    • enzyme binding Source: MGI
    • kinase activity Source: MGI
    • MAP kinase activity Source: UniProtKB
    • mitogen-activated protein kinase p38 binding Source: MGI
    • NFAT protein binding Source: BHF-UCL
    • protein C-terminus binding Source: MGI
    • protein kinase activity Source: MGI
    • protein phosphatase binding Source: MGI
    • protein serine/threonine kinase activity Source: MGI
    View the complete GO annotation on QuickGO ...

    GO - Biological processi

    • angiogenesis Source: MGI
    • apoptotic process Source: UniProtKB-KW
    • cartilage condensation Source: AgBase
    • cell morphogenesis Source: MGI
    • cellular response to DNA damage stimulus Source: MGI
    • cellular response to ionizing radiation Source: MGI
    • cellular response to lipopolysaccharide Source: MGI
    • cellular response to lipoteichoic acid Source: MGI
    • cellular response to tumor necrosis factor Source: MGI
    • cellular response to vascular endothelial growth factor stimulus Source: MGI
    • cellular response to virus Source: MGI
    • chondrocyte differentiation Source: MGI
    • DNA damage checkpoint Source: MGI
    • fatty acid oxidation Source: MGI
    • glucose metabolic process Source: MGI
    • intracellular signal transduction Source: UniProtKB
    • lipopolysaccharide-mediated signaling pathway Source: MGI
    • myoblast differentiation involved in skeletal muscle regeneration Source: MGI
    • negative regulation of canonical Wnt signaling pathway Source: AgBase
    • osteoclast differentiation Source: BHF-UCL
    • p38MAPK cascade Source: UniProtKB
    • peptidyl-serine phosphorylation Source: BHF-UCL
    • placenta development Source: MGI
    • positive regulation of brown fat cell differentiation Source: MGI
    • positive regulation of cardiac muscle cell proliferation Source: MGI
    • positive regulation of cyclase activity Source: MGI
    • positive regulation of cytokine secretion involved in immune response Source: CAFA
    • positive regulation of erythrocyte differentiation Source: MGI
    • positive regulation of gene expression Source: MGI
    • positive regulation of glucose import Source: MGI
    • positive regulation of interleukin-12 secretion Source: MGI
    • positive regulation of macrophage chemotaxis Source: CAFA
    • positive regulation of metallopeptidase activity Source: CAFA
    • positive regulation of myoblast differentiation Source: UniProtKB
    • positive regulation of myoblast fusion Source: UniProtKB
    • positive regulation of myotube differentiation Source: UniProtKB
    • positive regulation of protein import into nucleus Source: MGI
    • positive regulation of reactive oxygen species metabolic process Source: MGI
    • positive regulation of transcription by RNA polymerase II Source: MGI
    • protein autophosphorylation Source: MGI
    • protein phosphorylation Source: MGI
    • regulation of cytokine production involved in inflammatory response Source: MGI
    • regulation of ossification Source: MGI
    • regulation of transcription, DNA-templated Source: MGI
    • regulation of transcription by RNA polymerase II Source: UniProtKB
    • response to lipopolysaccharide Source: MGI
    • response to muramyl dipeptide Source: MGI
    • response to muscle stretch Source: MGI
    • signal transduction in response to DNA damage Source: MGI
    • skeletal muscle tissue development Source: MGI
    • stress-activated MAPK cascade Source: MGI
    • stress-induced premature senescence Source: MGI
    • striated muscle cell differentiation Source: MGI
    • transcription, DNA-templated Source: UniProtKB-KW
    • transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
    • vascular endothelial growth factor receptor signaling pathway Source: MGI
    View the complete GO annotation on QuickGO ...

    Keywordsi

    Molecular functionKinase, Serine/threonine-protein kinase, Transferase
    Biological processApoptosis, Stress response, Transcription, Transcription regulation
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24 3474
    ReactomeiR-MMU-168638 NOD1/2 Signaling Pathway
    R-MMU-171007 p38MAPK events
    R-MMU-198753 ERK/MAPK targets
    R-MMU-2559580 Oxidative Stress Induced Senescence
    R-MMU-375170 CDO in myogenesis
    R-MMU-376172 DSCAM interactions
    R-MMU-418592 ADP signalling through P2Y purinoceptor 1
    R-MMU-432142 Platelet sensitization by LDL
    R-MMU-4420097 VEGFA-VEGFR2 Pathway
    R-MMU-450302 activated TAK1 mediates p38 MAPK activation
    R-MMU-450341 Activation of the AP-1 family of transcription factors
    R-MMU-6798695 Neutrophil degranulation
    R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
    SABIO-RKiP47811

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 14 (EC:2.7.11.242 Publications)
    Short name:
    MAP kinase 14
    Short name:
    MAPK 14
    Alternative name(s):
    CRK1
    Mitogen-activated protein kinase p38 alpha
    Short name:
    MAP kinase p38 alpha
    Gene namesi
    Name:Mapk14
    Synonyms:Crk1, Csbp1, Csbp2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
    Proteomesi
    • UP000000589 Componenti: Chromosome 17

    Organism-specific databases

    MGIiMGI:1346865 Mapk14

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi180T → A: Phosphorylation blocked. 1 Publication1
    Mutagenesisi182Y → F: Phosphorylation blocked. 1 Publication1

    Chemistry databases

    ChEMBLiCHEMBL2336

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedBy similarity
    ChainiPRO_00001862922 – 360Mitogen-activated protein kinase 14Add BLAST359

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineBy similarity1
    Modified residuei2PhosphoserineBy similarity1
    Modified residuei16PhosphothreonineBy similarity1
    Modified residuei53N6-acetyllysineBy similarity1
    Modified residuei152N6-acetyllysineBy similarity1
    Modified residuei180PhosphothreonineCombined sources1 Publication1
    Modified residuei180Phosphothreonine; by MAP2K3, MAP2K4, MAP2K6 and autocatalysisBy similarity1
    Modified residuei182PhosphotyrosineCombined sources1 Publication1
    Modified residuei182Phosphotyrosine; by MAP2K3, MAP2K4, MAP2K6 and autocatalysisBy similarity1
    Modified residuei323Phosphotyrosine; by ZAP70By similarity1

    Post-translational modificationi

    Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory cytokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14 (By similarity).By similarity2 Publications
    Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3 (By similarity).By similarity
    Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway (By similarity).By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    EPDiP47811
    MaxQBiP47811
    PaxDbiP47811
    PeptideAtlasiP47811
    PRIDEiP47811

    PTM databases

    iPTMnetiP47811
    PhosphoSitePlusiP47811

    Expressioni

    Tissue specificityi

    Macrophages, monocytes, T- and B-lymphocytes. Isoform 2 is specifically expressed in kidney and liver.

    Gene expression databases

    BgeeiENSMUSG00000053436
    CleanExiMM_MAPK14
    ExpressionAtlasiP47811 baseline and differential
    GenevisibleiP47811 MM

    Interactioni

    Subunit structurei

    Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (By similarity). Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Interacts with PPM1D. Interacts with CDK5RAP3; recruits PPM1D to MAPK14 and may regulate its dephosphorylation (By similarity).By similarity6 Publications

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    View the complete GO annotation on QuickGO ...

    Protein-protein interaction databases

    BioGridi204969, 25 interactors
    DIPiDIP-31073N
    ELMiP47811
    IntActiP47811, 28 interactors
    MINTiP47811
    STRINGi10090.ENSMUSP00000004990

    Chemistry databases

    BindingDBiP47811

    Structurei

    Secondary structure

    1360
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi8 – 13Combined sources6
    Beta strandi16 – 21Combined sources6
    Beta strandi24 – 30Combined sources7
    Helixi31 – 33Combined sources3
    Beta strandi34 – 43Combined sources10
    Turni44 – 46Combined sources3
    Beta strandi49 – 54Combined sources6
    Helixi62 – 77Combined sources16
    Beta strandi87 – 90Combined sources4
    Helixi96 – 98Combined sources3
    Beta strandi103 – 107Combined sources5
    Beta strandi110 – 112Combined sources3
    Helixi113 – 117Combined sources5
    Helixi124 – 143Combined sources20
    Helixi153 – 155Combined sources3
    Beta strandi156 – 158Combined sources3
    Beta strandi164 – 166Combined sources3
    Beta strandi172 – 175Combined sources4
    Helixi176 – 180Combined sources5
    Beta strandi182 – 184Combined sources3
    Helixi185 – 188Combined sources4
    Helixi191 – 194Combined sources4
    Beta strandi197 – 199Combined sources3
    Helixi203 – 218Combined sources16
    Helixi228 – 239Combined sources12
    Helixi244 – 247Combined sources4
    Helixi253 – 261Combined sources9
    Helixi270 – 272Combined sources3
    Turni274 – 276Combined sources3
    Helixi279 – 288Combined sources10
    Helixi293 – 295Combined sources3
    Helixi299 – 303Combined sources5
    Helixi306 – 308Combined sources3
    Turni309 – 311Combined sources3
    Helixi314 – 316Combined sources3
    Helixi326 – 329Combined sources4
    Helixi334 – 346Combined sources13

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    		PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1LEWX-ray2.30A1-360[»]
    1LEZX-ray2.30A1-360[»]
    1YW2X-ray2.01A1-360[»]
    1YWRX-ray1.95A1-360[»]
    2EWAX-ray2.10A1-360[»]
    2GHLX-ray2.10A5-352[»]
    2GHMX-ray2.35A5-352[»]
    2GTMX-ray1.90A5-352[»]
    2GTNX-ray1.80A5-352[»]
    2OZAX-ray2.70B2-360[»]
    2PUUX-ray2.50A5-352[»]
    3P4KX-ray2.30A1-360[»]
    3P5KX-ray2.09A2-360[»]
    3P78X-ray2.30A2-360[»]
    3P79X-ray2.10A2-360[»]
    3P7AX-ray2.31A2-360[»]
    3P7BX-ray1.90A2-360[»]
    3P7CX-ray2.30A2-360[»]
    3PY3X-ray2.10A1-360[»]
    3TG1X-ray2.71A1-360[»]
    4KA3X-ray2.71A1-360[»]
    4LOOX-ray1.95A1-360[»]
    4LOPX-ray2.05A/B/C/D1-360[»]
    4LOQX-ray2.32A/B/C/D1-360[»]
    4TYHX-ray3.00B6-353[»]
    5LARX-ray1.50A1-360[»]
    5O90X-ray2.49A1-360[»]
    5UOJX-ray2.10A1-360[»]
    ProteinModelPortaliP47811
    SMRiP47811
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP47811

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini24 – 308Protein kinasePROSITE-ProRule annotationAdd BLAST285

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni106 – 111Inhibitor-binding6

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi180 – 182TXY3

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Curated

    Phylogenomic databases

    eggNOGiKOG0660 Eukaryota
    ENOG410XNY0 LUCA
    GeneTreeiENSGT00550000074271
    HOVERGENiHBG014652
    InParanoidiP47811
    KOiK04441
    OMAiDVIDTIC
    OrthoDBiEOG091G08QL
    PhylomeDBiP47811
    TreeFamiTF105100

    Family and domain databases

    CDDicd07877 STKc_p38alpha, 1 hit
    InterProiView protein in InterPro
    IPR011009 Kinase-like_dom_sf
    IPR003527 MAP_kinase_CS
    IPR008352 MAPK_p38-like
    IPR038784 p38alpha
    IPR000719 Prot_kinase_dom
    IPR017441 Protein_kinase_ATP_BS
    PfamiView protein in Pfam
    PF00069 Pkinase, 1 hit
    PRINTSiPR01773 P38MAPKINASE
    SMARTiView protein in SMART
    SM00220 S_TKc, 1 hit
    SUPFAMiSSF56112 SSF56112, 1 hit
    PROSITEiView protein in PROSITE
    PS01351 MAPK, 1 hit
    PS00107 PROTEIN_KINASE_ATP, 1 hit
    PS50011 PROTEIN_KINASE_DOM, 1 hit

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: P47811-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGHRV 
            60         70         80         90        100
    AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN 
           110        120        130        140        150
    DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD 
           160        170        180        190        200
    LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY 
           210        220        230        240        250
    NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI 
           260        270        280        290        300
    SSESARNYIQ SLAQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA 
           310        320        330        340        350
    QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP 
           360
    PPLDQEEMES
    Length:360
    Mass (Da):41,287
    Last modified:January 23, 2007 - v3
    Checksum:iDFB03EBCE97BB51A
    GO
    Isoform 2 (identifier: P47811-2) [UniParc]FASTAAdd to basket
    Also known as: Piccolo

    The sequence of this isoform differs from the canonical sequence as follows:
         255-278: ARNYIQSLAQMPKMNFANVFIGAN → DAKP
         279-360: Missing.

    Show »
    Length:258
    Mass (Da):29,637
    Checksum:iABBBDF65DC32AF03
    GO
    Isoform 3 (identifier: P47811-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         230-254: DQLKLILRLVGTPGAELLKKISSES → NQLQQIMRLTGTPPAYLINRMPSHE

    Show »
    Length:360
    Mass (Da):41,487
    Checksum:iF1F20352F76590BD
    GO
    Isoform 4 (identifier: P47811-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-77: Missing.

    Show »
    Length:283
    Mass (Da):32,327
    Checksum:i895D71CB69550519
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti98E → G in AAF06348 (Ref. 7) Curated1
    Sequence conflicti107 – 108HL → LS in AAF06348 (Ref. 7) Curated2
    Sequence conflicti115N → R in AAF06348 (Ref. 7) Curated1
    Sequence conflicti124D → G in AAF06348 (Ref. 7) Curated1
    Sequence conflicti159 – 162NEDC → TQVI in AAF06348 (Ref. 7) Curated4
    Sequence conflicti166I → L in AAF06348 (Ref. 7) Curated1
    Sequence conflicti202Q → R in AAF06348 (Ref. 7) Curated1
    Sequence conflicti211 – 212CI → GF in AAF06348 (Ref. 7) Curated2
    Sequence conflicti224P → L in AAF06348 (Ref. 7) Curated1
    Sequence conflicti271A → P in AAF06348 (Ref. 7) Curated1
    Sequence conflicti299A → V in AAF06348 (Ref. 7) Curated1
    Sequence conflicti315D → Y in AAF06348 (Ref. 7) Curated1
    Isoform 3 (identifier: P47811-3)
    Sequence conflicti238L → M in BAA19741 (Ref. 2) Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0223591 – 77Missing in isoform 4. 1 PublicationAdd BLAST77
    Alternative sequenceiVSP_007544230 – 254DQLKL…ISSES → NQLQQIMRLTGTPPAYLINR MPSHE in isoform 3. 3 PublicationsAdd BLAST25
    Alternative sequenceiVSP_004846255 – 278ARNYI…FIGAN → DAKP in isoform 2. 1 PublicationAdd BLAST24
    Alternative sequenceiVSP_007545279 – 360Missing in isoform 2. 1 PublicationAdd BLAST82

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		U10871 mRNA Translation: AAA20888.1
    D83073 mRNA Translation: BAA19741.1
    AF128892 mRNA Translation: AAF34818.1
    AK151348 mRNA Translation: BAE30324.1
    AK153025 mRNA Translation: BAE31659.1
    AK089059 mRNA Translation: BAC40726.1
    AK133684 mRNA Translation: BAE21782.1
    CT009661 Genomic DNA No translation available.
    BC012235 mRNA Translation: AAH12235.1
    AF195850 mRNA Translation: AAF06348.1
    X65067 mRNA Translation: CAA46200.1
    CCDSiCCDS28583.1 [P47811-1]
    CCDS50048.1 [P47811-3]
    CCDS50049.1 [P47811-4]
    PIRiI49066
    RefSeqiNP_001161980.1, NM_001168508.1 [P47811-3]
    NP_001161985.1, NM_001168513.1 [P47811-4]
    NP_001161986.1, NM_001168514.1 [P47811-4]
    NP_036081.1, NM_011951.3 [P47811-1]
    UniGeneiMm.311337

    Genome annotation databases

    EnsembliENSMUST00000004990; ENSMUSP00000004990; ENSMUSG00000053436 [P47811-3]
    ENSMUST00000062694; ENSMUSP00000061958; ENSMUSG00000053436 [P47811-1]
    ENSMUST00000114752; ENSMUSP00000110400; ENSMUSG00000053436 [P47811-4]
    ENSMUST00000114754; ENSMUSP00000110402; ENSMUSG00000053436 [P47811-4]
    GeneIDi26416
    KEGGimmu:26416
    UCSCiuc008brl.2 mouse [P47811-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiMK14_MOUSE
    AccessioniPrimary (citable) accession number: P47811
    Secondary accession number(s): B2KF37
    , B2KF38, O08666, Q3U6R5, Q3UZS3, Q8C289, Q9JLV8, Q9QZ80
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: June 20, 2018
    This is version 206 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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