P47806 (GLI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein GLI1

Alternative name(s):
Glioma-associated oncogene homolog

Gene names

Name:	Gli1
Synonyms:	Gli

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1111 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation By similarity.
Subunit structure	Interacts with ZIC1; the interaction enhances transcription activation By similarity. Interacts with KIF7. Ref.6
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1 By similarity.
Developmental stage	Is detected on days 10 through 18 of embryonic development. During gestation it is detected in meckels precartilage mesenchyme, the basis occipitus, rib mesenchymal condensations, primordial vertebral bodies, digital mesenchymal condensations in forefoot and hindfoot plates, the ependymal layer of the spinal cord, and the mesoderm of the gastrointestinal tract. Expression persists throughout gestation in developing bone and cartilage of the extremities, the ribs, and the vertebral bodies as well as the gastrointestinal tract mesoderm.
Post-translational modification	Phosphorylated in vitro by ULK3. Acetylation at Lys-520 down-regulates transcriptional activity. Deacetylated by HDAC1.
Sequence similarities	Belongs to the GLI C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Disease	Proto-oncogene
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cerebellar cortex morphogenesis Inferred from genetic interaction PubMed 16571625. Source: MGI dorsal/ventral pattern formation Inferred from genetic interaction PubMed 16342201. Source: MGI epidermal cell differentiation Inferred from electronic annotation. Source: Compara lung development Inferred from genetic interaction PubMed 10725236. Source: MGI negative regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara notochord regression Inferred from genetic interaction PubMed 10725236. Source: MGI osteoblast differentiation Inferred from electronic annotation. Source: Compara pituitary gland development Inferred from genetic interaction PubMed 10725236. Source: MGI positive regulation of DNA replication Inferred from electronic annotation. Source: Compara positive regulation of smoothened signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16611981PubMed 17442700. Source: MGI proximal/distal pattern formation Inferred from genetic interaction PubMed 16342201. Source: MGI smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation Inferred from genetic interaction PubMed 16571625. Source: MGI spermatogenesis Inferred from direct assay PubMed 11717126. Source: MGI ventral midline development Inferred from genetic interaction PubMed 10725236. Source: MGI
Cellular_component	cytoplasm Inferred from direct assay PubMed 11717126PubMed 11821712PubMed 19684112. Source: MGI cytosol Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 11717126PubMed 16254602PubMed 16316410PubMed 16489008PubMed 17442700PubMed 19684112. Source: MGI primary cilium Inferred from direct assay PubMed 19684112. Source: MGI
Molecular_function	DNA binding Inferred from direct assay PubMed 15614767. Source: MGI RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 17442700. Source: MGI chromatin binding Inferred from direct assay PubMed 16396903PubMed 17442700. Source: MGI microtubule binding Inferred from direct assay PubMed 11717126. Source: MGI transcription regulatory region DNA binding Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1111	1111	Zinc finger protein GLI1		PRO_0000047198
Regions
	Zinc finger	238 – 263	26	C2H2-type 1
	Zinc finger	271 – 298	28	C2H2-type 2
	Zinc finger	304 – 328	25	C2H2-type 3
	Zinc finger	334 – 359	26	C2H2-type 4
	Zinc finger	365 – 390	26	C2H2-type 5
	Compositional bias	1042 – 1059	18	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	520	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	154	1	V → L in AAC09169. Ref.1
	Sequence conflict	170	1	H → Y in AAC09169. Ref.1
	Sequence conflict	173	1	S → A in BAA85004. Ref.2
	Sequence conflict	179	1	T → I in AAC09169. Ref.1
	Sequence conflict	194	1	P → R in AAC09169. Ref.1
	Sequence conflict	210	1	T → I in BAA85004. Ref.2
	Sequence conflict	271	1	F → S in AAC09169. Ref.1
	Sequence conflict	474	1	Missing in AAC09169. Ref.1
	Sequence conflict	567 – 569	3	FPP → LPT in AAC09169. Ref.1
	Sequence conflict	707	1	E → D in AAC09169. Ref.1
	Sequence conflict	777	1	Missing in AAC09169. Ref.1
	Sequence conflict	864	1	G → V in AAC09169. Ref.1
	Sequence conflict	919 – 920	2	GL → RA in AAC09169. Ref.1
	Sequence conflict	936	1	S → Y in AAC09169. Ref.1
	Sequence conflict	947	1	Missing in AAC09169. Ref.1
	Sequence conflict	951 – 952	2	AA → RR in AAC09169. Ref.1
	Sequence conflict	967	1	G → R in AAC09169. Ref.1
	Sequence conflict	990	1	P → A in AAC09169. Ref.1
	Sequence conflict	1029	1	A → P in AAC09169. Ref.1
	Sequence conflict	1062 – 1063	2	QG → R in AAC09169. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47806 [UniParc]. Last modified October 3, 2012. Version 4. Checksum: 8A83B254DCBB9BDC	FASTA	1,111	118,560
	10 20 30 40 50 60 MFNPMTPPQV NSYSEPCCLR PLHSQGVPSM GTEGLSGLPF CHQANFMSGS QGYGAARETS 70 80 90 100 110 120 SCTEGSLFPP PPPPRSSVKL TKKRALSISP LSDASLDLQT VIRTSPSSLV AFINSRCTSP 130 140 150 160 170 180 GGSYGHLSIG TMSPSLGFPP QMSHQKGTSP PYGVQPCVPH DSTRGSMMLH PQSRGPRATC 190 200 210 220 230 240 QLKSELDMMV GKCPEDPLEG DMSSPNSTGT QDHLLGMLDG REDLEREEKP EPESVYETDC 250 260 270 280 290 300 RWDGCSQEFD SQEQLVHHIN SEHIHGERKE FVCHWGGCSR ELRPFKAQYM LVVHMRRHTG 310 320 330 340 350 360 EKPHKCTFEG CRKSYSRLEN LKTHLRSHTG EKPYMCEQEG CSKAFSNASD RAKHQNRTHS 370 380 390 400 410 420 NEKPYVCKLP GCTKRYTDPS SLRKHVKTVH GPDAHVTKRH RGDGPLPRAQ PLSTVEPKRE 430 440 450 460 470 480 REGGSGREES RLTVPESAMP QQSPGAQSSC SSDHSPAGSA ANTDSGVEMA GNAGGSTEDL 490 500 510 520 530 540 SSLDEGPCVS ATGLSTLRRL ENLRLDQLHQ LRPIGSRGLK LPSLTHAGAP VSRRLGPPVS 550 560 570 580 590 600 LDRRSSSSSS MSSAYTVSRR SSLASPFPPG TPPENGASSL PGLTPAQHYM LRARYASARG 610 620 630 640 650 660 SGTPPTAAHS LDRMGGLSVP PWRSRTEYPG YNPNAGVTRR ASDPARAADH PAPARVQRFK 670 680 690 700 710 720 SLGCVHTPPS VATGRNFDPH HPTSVYSPQP PSITENVAMD TRGLQEEPEV GTSVMGNGLN 730 740 750 760 770 780 PYMDFSSTDT LGYGGPEGTA AEPYEARGPG SLPLGPGPPT NYGPGHCAQQ VSYPDPTPEN 790 800 810 820 830 840 WGEFPSHAGV YPSNKAPGAA YSQCPRLEHY GQVQVKPEQG CPVGSDSTGL APCLNAHPSE 850 860 870 880 890 900 GSPGPQPLFS HHPQLPQPQY PQSGPYPQPP HGYLSTEPRL GLNFNPSSSH STGQLKAQLV 910 920 930 940 950 960 CNYVQSQQEL LWEGRNRGGL PNQELPYQSP KFLGGSQVSQ SPAKTPAAAA AAYGSGFAPA 970 980 990 1000 1010 1020 SANHKSGSYP APSPCHETFT VGVNRPSHRP AAPPRLLPPL SPCYGPLKVG DTNPSCGHPE 1030 1040 1050 1060 1070 1080 VGRLGAGPAL YPPPEGQVCN ALDSLDLDNT QLDFVAILDE AQGLSPPLSH EQGDSSKNTP 1090 1100 1110 SPSGPPNMAV GNMSVLLGSL PGETQFLNSS A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the promoter region and genomic organization of GLI, a member of the Sonic hedgehog-Patched signaling pathway." Liu C.Z., Yang J.T., Yoon J.W., Walterhouse D., Iannaccone P. Gene 209:1-11(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling." Sasaki H., Nishizaki Y., Hui C., Nakafuku M., Kondoh H. Development 126:3915-3924(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"gli, a zinc finger transcription factor and oncogene, is expressed during normal mouse development." Waterhouse D., Ahmed M., Slusarski D., Kalamaras J., Boucher D., Holmgren R., Iannaccone P. Dev. Dyn. 196:91-102(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 272-837. Strain: CD-1. Tissue: Embryo.
[6]	"The mammalian Cos2 homolog Kif7 plays an essential role in modulating Hh signal transduction during development." Endoh-Yamagami S., Evangelista M., Wilson D., Wen X., Theunissen J.W., Phamluong K., Davis M., Scales S.J., Solloway M.J., de Sauvage F.J., Peterson A.S. Curr. Biol. 19:1320-1326(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KIF7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF026305 mRNA. Translation: AAC09169.1. AB025922 mRNA. Translation: BAA85004.1. AC114678 Genomic DNA. No translation available. CH466578 Genomic DNA. Translation: EDL24501.1.
IPI	IPI00112331.
RefSeq	NP_034426.2. NM_010296.2.
UniGene	Mm.391450.
3D structure databases
ProteinModelPortal	P47806.
SMR	P47806. Positions 237-449.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47806. 1 interaction.
STRING	10090.ENSMUSP00000026474.
PTM databases
PhosphoSite	P47806.
Proteomic databases
PaxDb	P47806.
PRIDE	P47806.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026474; ENSMUSP00000026474; ENSMUSG00000025407.
GeneID	14632.
KEGG	mmu:14632.
UCSC	uc007hjf.1. mouse.
Organism-specific databases
CTD	2735.
MGI	MGI:95727. Gli1.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00700000104153.
HOGENOM	HOG000290688.
HOVERGEN	HBG080668.
InParanoid	P47806.
KO	K16797.
OMA	CPRLEHY.
OrthoDB	EOG4T1HKP.
Gene expression databases
CleanEx	MM_GLI1.
Genevestigator	P47806.
GermOnline	ENSMUSG00000025407. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 5 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 5 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 4 hits. PS50157. ZINC_FINGER_C2H2_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P47806.
ChEMBL	CHEMBL5007.
NextBio	286482.
SOURCE	Search...

Entry information

Entry name

GLI1_MOUSE

Accession

Primary (citable) accession number: P47806
Secondary accession number(s): G5E857, Q9QYK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 115 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program