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P47806 (GLI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein GLI1
Alternative name(s):
Glioma-associated oncogene homolog
Gene names
Name:Gli1
Synonyms:Gli
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1111 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation By similarity.

Subunit structure

Interacts with ZIC1; the interaction enhances transcription activation By similarity. Interacts with KIF7. Ref.6

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1 By similarity.

Developmental stage

Is detected on days 10 through 18 of embryonic development. During gestation it is detected in meckels precartilage mesenchyme, the basis occipitus, rib mesenchymal condensations, primordial vertebral bodies, digital mesenchymal condensations in forefoot and hindfoot plates, the ependymal layer of the spinal cord, and the mesoderm of the gastrointestinal tract. Expression persists throughout gestation in developing bone and cartilage of the extremities, the ribs, and the vertebral bodies as well as the gastrointestinal tract mesoderm.

Post-translational modification

Phosphorylated in vitro by ULK3.

Acetylation at Lys-520 down-regulates transcriptional activity. Deacetylated by HDAC1.

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DiseaseProto-oncogene
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
Developmental protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcerebellar cortex morphogenesis

Inferred from genetic interaction PubMed 16571625. Source: MGI

dorsal/ventral pattern formation

Inferred from genetic interaction PubMed 16342201. Source: MGI

epidermal cell differentiation

Inferred from electronic annotation. Source: Ensembl

lung development

Inferred from genetic interaction PubMed 10725236. Source: MGI

negative regulation of canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

notochord regression

Inferred from genetic interaction PubMed 10725236. Source: MGI

osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

pituitary gland development

Inferred from genetic interaction PubMed 10725236. Source: MGI

positive regulation of DNA replication

Inferred from electronic annotation. Source: Ensembl

positive regulation of smoothened signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16611981PubMed 17442700. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 17035233. Source: UniProtKB

proximal/distal pattern formation

Inferred from genetic interaction PubMed 16342201. Source: MGI

signal transduction

Traceable author statement PubMed 11717126. Source: MGI

smoothened signaling pathway

Inferred from direct assay PubMed 19124651PubMed 9769173. Source: MGI

smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation

Inferred from genetic interaction PubMed 16571625. Source: MGI

spermatogenesis

Inferred from direct assay PubMed 11717126. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 17442700. Source: GOC

ventral midline development

Inferred from genetic interaction PubMed 10725236. Source: MGI

   Cellular_componentcilium

Inferred from direct assay PubMed 16254602. Source: MGI

cytoplasm

Inferred from direct assay PubMed 11717126PubMed 11821712PubMed 19684112. Source: MGI

cytosol

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 11717126PubMed 16254602PubMed 16316410PubMed 16489008PubMed 17442700PubMed 19684112. Source: MGI

primary cilium

Inferred from direct assay PubMed 19684112. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15614767. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 17442700. Source: MGI

chromatin binding

Inferred from direct assay PubMed 16396903PubMed 17442700. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule binding

Inferred from direct assay PubMed 11717126. Source: MGI

protein binding

Inferred from physical interaction Ref.6. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11111111Zinc finger protein GLI1
PRO_0000047198

Regions

Zinc finger238 – 26326C2H2-type 1
Zinc finger271 – 29828C2H2-type 2
Zinc finger304 – 32825C2H2-type 3
Zinc finger334 – 35926C2H2-type 4
Zinc finger365 – 39026C2H2-type 5
Compositional bias1042 – 105918Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue5201N6-acetyllysine By similarity

Experimental info

Sequence conflict1541V → L in AAC09169. Ref.1
Sequence conflict1701H → Y in AAC09169. Ref.1
Sequence conflict1731S → A in BAA85004. Ref.2
Sequence conflict1791T → I in AAC09169. Ref.1
Sequence conflict1941P → R in AAC09169. Ref.1
Sequence conflict2101T → I in BAA85004. Ref.2
Sequence conflict2711F → S in AAC09169. Ref.1
Sequence conflict4741Missing in AAC09169. Ref.1
Sequence conflict567 – 5693FPP → LPT in AAC09169. Ref.1
Sequence conflict7071E → D in AAC09169. Ref.1
Sequence conflict7771Missing in AAC09169. Ref.1
Sequence conflict8641G → V in AAC09169. Ref.1
Sequence conflict919 – 9202GL → RA in AAC09169. Ref.1
Sequence conflict9361S → Y in AAC09169. Ref.1
Sequence conflict9471Missing in AAC09169. Ref.1
Sequence conflict951 – 9522AA → RR in AAC09169. Ref.1
Sequence conflict9671G → R in AAC09169. Ref.1
Sequence conflict9901P → A in AAC09169. Ref.1
Sequence conflict10291A → P in AAC09169. Ref.1
Sequence conflict1062 – 10632QG → R in AAC09169. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P47806 [UniParc].

Last modified October 3, 2012. Version 4.
Checksum: 8A83B254DCBB9BDC

FASTA1,111118,560
        10         20         30         40         50         60 
MFNPMTPPQV NSYSEPCCLR PLHSQGVPSM GTEGLSGLPF CHQANFMSGS QGYGAARETS 

        70         80         90        100        110        120 
SCTEGSLFPP PPPPRSSVKL TKKRALSISP LSDASLDLQT VIRTSPSSLV AFINSRCTSP 

       130        140        150        160        170        180 
GGSYGHLSIG TMSPSLGFPP QMSHQKGTSP PYGVQPCVPH DSTRGSMMLH PQSRGPRATC 

       190        200        210        220        230        240 
QLKSELDMMV GKCPEDPLEG DMSSPNSTGT QDHLLGMLDG REDLEREEKP EPESVYETDC 

       250        260        270        280        290        300 
RWDGCSQEFD SQEQLVHHIN SEHIHGERKE FVCHWGGCSR ELRPFKAQYM LVVHMRRHTG 

       310        320        330        340        350        360 
EKPHKCTFEG CRKSYSRLEN LKTHLRSHTG EKPYMCEQEG CSKAFSNASD RAKHQNRTHS 

       370        380        390        400        410        420 
NEKPYVCKLP GCTKRYTDPS SLRKHVKTVH GPDAHVTKRH RGDGPLPRAQ PLSTVEPKRE 

       430        440        450        460        470        480 
REGGSGREES RLTVPESAMP QQSPGAQSSC SSDHSPAGSA ANTDSGVEMA GNAGGSTEDL 

       490        500        510        520        530        540 
SSLDEGPCVS ATGLSTLRRL ENLRLDQLHQ LRPIGSRGLK LPSLTHAGAP VSRRLGPPVS 

       550        560        570        580        590        600 
LDRRSSSSSS MSSAYTVSRR SSLASPFPPG TPPENGASSL PGLTPAQHYM LRARYASARG 

       610        620        630        640        650        660 
SGTPPTAAHS LDRMGGLSVP PWRSRTEYPG YNPNAGVTRR ASDPARAADH PAPARVQRFK 

       670        680        690        700        710        720 
SLGCVHTPPS VATGRNFDPH HPTSVYSPQP PSITENVAMD TRGLQEEPEV GTSVMGNGLN 

       730        740        750        760        770        780 
PYMDFSSTDT LGYGGPEGTA AEPYEARGPG SLPLGPGPPT NYGPGHCAQQ VSYPDPTPEN 

       790        800        810        820        830        840 
WGEFPSHAGV YPSNKAPGAA YSQCPRLEHY GQVQVKPEQG CPVGSDSTGL APCLNAHPSE 

       850        860        870        880        890        900 
GSPGPQPLFS HHPQLPQPQY PQSGPYPQPP HGYLSTEPRL GLNFNPSSSH STGQLKAQLV 

       910        920        930        940        950        960 
CNYVQSQQEL LWEGRNRGGL PNQELPYQSP KFLGGSQVSQ SPAKTPAAAA AAYGSGFAPA 

       970        980        990       1000       1010       1020 
SANHKSGSYP APSPCHETFT VGVNRPSHRP AAPPRLLPPL SPCYGPLKVG DTNPSCGHPE 

      1030       1040       1050       1060       1070       1080 
VGRLGAGPAL YPPPEGQVCN ALDSLDLDNT QLDFVAILDE AQGLSPPLSH EQGDSSKNTP 

      1090       1100       1110 
SPSGPPNMAV GNMSVLLGSL PGETQFLNSS A 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the promoter region and genomic organization of GLI, a member of the Sonic hedgehog-Patched signaling pathway."
Liu C.Z., Yang J.T., Yoon J.W., Walterhouse D., Iannaccone P.
Gene 209:1-11(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling."
Sasaki H., Nishizaki Y., Hui C., Nakafuku M., Kondoh H.
Development 126:3915-3924(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"gli, a zinc finger transcription factor and oncogene, is expressed during normal mouse development."
Waterhouse D., Ahmed M., Slusarski D., Kalamaras J., Boucher D., Holmgren R., Iannaccone P.
Dev. Dyn. 196:91-102(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 272-837.
Strain: CD-1.
Tissue: Embryo.
[6]"The mammalian Cos2 homolog Kif7 plays an essential role in modulating Hh signal transduction during development."
Endoh-Yamagami S., Evangelista M., Wilson D., Wen X., Theunissen J.W., Phamluong K., Davis M., Scales S.J., Solloway M.J., de Sauvage F.J., Peterson A.S.
Curr. Biol. 19:1320-1326(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KIF7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF026305 mRNA. Translation: AAC09169.1.
AB025922 mRNA. Translation: BAA85004.1.
AC114678 Genomic DNA. No translation available.
CH466578 Genomic DNA. Translation: EDL24501.1.
CCDSCCDS24238.1.
RefSeqNP_034426.2. NM_010296.2.
UniGeneMm.391450.

3D structure databases

ProteinModelPortalP47806.
SMRP47806. Positions 237-444.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199942. 5 interactions.
IntActP47806. 1 interaction.
STRING10090.ENSMUSP00000026474.

Chemistry

BindingDBP47806.
ChEMBLCHEMBL5007.

PTM databases

PhosphoSiteP47806.

Proteomic databases

PaxDbP47806.
PRIDEP47806.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026474; ENSMUSP00000026474; ENSMUSG00000025407.
GeneID14632.
KEGGmmu:14632.
UCSCuc007hjf.1. mouse.

Organism-specific databases

CTD2735.
MGIMGI:95727. Gli1.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00740000115106.
HOGENOMHOG000290688.
HOVERGENHBG080668.
InParanoidP47806.
KOK16797.
OMACPRLEHY.
OrthoDBEOG7X6KZ8.
TreeFamTF350216.

Gene expression databases

ArrayExpressP47806.
CleanExMM_GLI1.
GenevestigatorP47806.

Family and domain databases

Gene3D3.30.160.60. 5 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio286482.
PROP47806.
SOURCESearch...

Entry information

Entry nameGLI1_MOUSE
AccessionPrimary (citable) accession number: P47806
Secondary accession number(s): G5E857, Q9QYK1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 126 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot