P47796 (CTRA_GADMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Chymotrypsin A EC=3.4.21.1
Organism	Gadus morhua (Atlantic cod)
Taxonomic identifier	8049 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Paracanthopterygii › Gadiformes › Gadidae › Gadus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.2
	Chain	19 – 263	245	Chymotrypsin A		PRO_0000027646
Regions
	Domain	34 – 261	228	Peptidase S1
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	213	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	19 ↔ 140		By similarity
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	154 ↔ 219		By similarity
	Disulfide bond	186 ↔ 200		By similarity
	Disulfide bond	209 ↔ 238		By similarity
Experimental info
	Sequence conflict	21	1	R → S AA sequence Ref.2
	Sequence conflict	25	1	S → Q AA sequence Ref.2
	Sequence conflict	29	1	T → S AA sequence Ref.2
	Sequence conflict	44	1	S → T AA sequence Ref.2
	Sequence conflict	46	1	S → Y AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47796 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 47AAC699A0A64FBB	FASTA	263	28,294
	10 20 30 40 50 60 MGHEVDSVLP GLFRRTYGCG RPAISPVITG YSRIVNGEEA VPHSWSWQVS LQDQTGFHFC 70 80 90 100 110 120 GGSLINENWV VTAAHCNVKN YHRVVLGEHD RSSNSEGVQV MTVGQVFKHP RYNGFTINND 130 140 150 160 170 180 ILLVKLATPA TLNMRVSPVC LAETDDVFEG GMKCVTSGWG LTRYNAADTP ALLQQAALPL 190 200 210 220 230 240 LTNEQCKKFW GNKISDLMIC AGAAGASSCM GDSGGPLVCQ KAGSWTLVGI VSWGSGTCTP 250 260 TMPGVYARVT ELRAWVDQTI AAN

References

[1]	"Atlantic cod cDNA encoding a psychrophilic chymotrypsinogen." Gudmundsdottir A., Oskarsson S., Eakin A.E., Craik C.S., Bjarnason J.B. Biochim. Biophys. Acta 1219:211-214(1994) [PubMed: 8086467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pyloric caecum.
[2]	"Structural and kinetic properties of chymotrypsin from Atlantic cod (Gadus morhua). Comparison with bovine chymotrypsin." Asgeirsson B., Bjarnason J.B. Comp. Biochem. Physiol. 99B:327-335(1991) [PubMed: 1764912] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-30 AND 34-49. Tissue: Pyloric caecum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78490 mRNA. Translation: CAA55242.1.
PIR	S47537.
3D structure databases
ProteinModelPortal	P47796.
SMR	P47796. Positions 19-263.
ModBase	Search...
Protein family/group databases
MEROPS	S01.437.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG013304.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTRA_GADMO

Accession

Primary (citable) accession number: P47796

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program