P47793 (WNT4A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-4a

Gene names

Name:	wnt4a
Synonyms:	wnt-4, wnt4

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Inhibits cell movements.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Caudal forebrain and neural keel, the floor plate, the gill slit and the developing pronephros.
Developmental stage	First detected at the end of gastrulation in the anterior neuroectoderm. Expression increases in the developing brain during somitogenesis but has gone by 36 hours. Expression in the floor plate begins at the 10 somite stage and persists at 72 hours. Ref.2
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell migration involved in gastrulation Inferred from mutant phenotype. Source: ZFIN cell-cell signaling Non-traceable author statement. Source: UniProtKB habenula development Inferred from mutant phenotype. Source: ZFIN positive regulation of transcription, DNA-dependent Inferred from physical interaction. Source: ZFIN
Cellular component	proteinaceous extracellular matrix Non-traceable author statement. Source: UniProtKB
Molecular function	frizzled binding Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 352	330	Protein Wnt-4a		PRO_0000041424
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	245 – 246	2	EL → DV in AAA96518. Ref.2
	Sequence conflict	335	1	C → S in AAA96518. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47793 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D63CBF9F00886633	FASTA	352	39,431
	10 20 30 40 50 60 MSSEYLIRSL LMLFLALFSA NASNWLYLAK LSSVGSISDE ETCEKLRGLI QRQVQICKRN 70 80 90 100 110 120 VEVMDAVRRG AQLAIDECQY QFRNRRWNCS TLESVPVFGK VVTQGTREAA FVYAISAASV 130 140 150 160 170 180 AFAVTRACSS GELDKCGCDR NVHGVSPEGF QWSGCSDNIA YGVAFSQSFV DIRERSKGQS 190 200 210 220 230 240 SNRALMNLHN NEAGRKAILN HMRVECKCHG VSGSCEVKTC WKAMPPFRKV GNVIKEKFDG 250 260 270 280 290 300 ATEVELRKVG TTKVLVPRNS QFKPHTDEDL VYLDPSPDFC EHDPRTPGIM GTAGRFCNKT 310 320 330 340 350 SKAIDGCELM CCGRGFHTEE VEVVDRCSCK FHWCCYVKCK QCRKMVEMHT CR

References

[1]	"Wnt4 affects morphogenesis when misexpressed in the zebrafish embryo." Ungar A.R., Kelly G.M., Moon R.T. Mech. Dev. 52:153-164(1995) [PubMed: 8541205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Three Wnt genes expressed in a wide variety of tissues during development of the zebrafish, Danio rerio: developmental and evolutionary perspectives." Blader P., Straehle U., Ingham P.W. Dev. Genes Evol. 206:3-13(1996) Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25141 mRNA. Translation: AAA96004.1. U51267 mRNA. Translation: AAA96518.1.
IPI	IPI00500843.
RefSeq	NP_001035477.1. NM_001040387.1.
UniGene	Dr.385.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P47793.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000105133; ENSDARP00000095904; ENSDARG00000071208.
GeneID	30123.
KEGG	dre:30123.
Organism-specific databases
CTD	30123.
ZFIN	ZDB-GENE-980526-352. wnt4a.
Phylogenomic databases
eggNOG	fiNOG04363.
HOGENOM	HBG446188.
HOVERGEN	HBG001595.
InParanoid	P47793.
OMA	VERCSCK.
OrthoDB	EOG49P9ZP.
Gene expression databases
ArrayExpress	P47793.
Bgee	P47793.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009142. Wnt4. IPR018161. Wnt_grthfactor_CS. [Graphical view]
KO	K00408.
PANTHER	PTHR12027. Wnt. 1 hit. PTHR12027:SF19. Wnt4. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01844. WNT4PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WNT4A_DANRE

Accession

Primary (citable) accession number: P47793
Secondary accession number(s): Q92049

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program