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P47788 (THOP1_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thimet oligopeptidase

EC=3.4.24.15
Alternative name(s):
Endopeptidase 24.15
Gene names
Name:THOP1
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length687 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation.

Catalytic activity

Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Monomer.

Subcellular location

Cytoplasm.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the peptidase M3 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 687687Thimet oligopeptidase
PRO_0000078154

Sites

Active site4741 By similarity
Metal binding4731Zinc; catalytic By similarity
Metal binding4771Zinc; catalytic By similarity
Metal binding4801Zinc; catalytic By similarity

Amino acid modifications

Modified residue2781Phosphotyrosine By similarity
Modified residue5381N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P47788 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 70AEF85C6C466929

FASTA68778,144
        10         20         30         40         50         60 
MKPPAACAGD ALDVAAPCSA VNYLRWDLSA QQIGELTTEL IEQTKRVYDR VGTQELQDVS 

        70         80         90        100        110        120 
YENTLKALAD VEVSYTVQRN ILDFPQHVSP CKDIRTASTE ADKKLSEFDV EMSMRQDVYQ 

       130        140        150        160        170        180 
RIVWLQEKVQ KDSLRPEAAR YLERLIKLGR RNGLHLPKET QEKIKSIKKK LSLLCIDFNK 

       190        200        210        220        230        240 
NLNEDTTFLP VTREELGGLP EDFLNSLEKT EDEKLKVTLK YPHYFPLLKK CHVPETRRKV 

       250        260        270        280        290        300 
EEAFNCRCKE ENCAILRELV RLRAQKSSLL GFSTHADYVL EMNMAKTSQV VATFLDELAQ 

       310        320        330        340        350        360 
KLKPLGEQER AVILELKKAE CTKRGLDFDG RINAWDMRYY MNQVEETRYR VDQNLLKEYF 

       370        380        390        400        410        420 
PMQVVTRGLL GIYQELLGLT FHLEEGAAVW HEDVMLYSVR DAASGKVIGK FYLDLYPREG 

       430        440        450        460        470        480 
KYGHAACFGL QPGCLRQDGS RQIAIAAMVA NFTKPTPDAP SLLQHDEVET YFHEFGHVMH 

       490        500        510        520        530        540 
QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEAEPLLR MSQHYRTGSA IPQELLEKLI 

       550        560        570        580        590        600 
KSRQANTGLF NLRQIVLAKV DQALHTQTAA DPAEEYARLC QEILGVPATP GTNMPATFGH 

       610        620        630        640        650        660 
LAGGYDAQYY GYLWSEVYSA DMFHTRFKQE GILSGKVGMD YRSCILRPGG SEDASVMLKL 

       670        680 
FLGRDPKQDA FLLSKGLQVE GCEPPAS 

« Hide

References

[1]"Cloning, amino acid sequence and tissue distribution of porcine thimet oligopeptidase. A comparison with soluble angiotensin-binding protein."
Kato A., Sugiura N., Hagiwara H., Hirose S.
Eur. J. Biochem. 221:159-165(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Targeting of endopeptidase 24.16 to different subcellular compartments by alternative promoter usage."
Kato A., Sugiura N., Saruta Y., Hosoiri T., Yasue H., Hirose S.
J. Biol. Chem. 272:15313-15322(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D21871 mRNA. Translation: BAA04882.1.
AB000438 Genomic DNA. Translation: BAA19107.1.
PIRS43250.
RefSeqNP_999388.1. NM_214223.2.
UniGeneSsc.16161.

3D structure databases

ProteinModelPortalP47788.
SMRP47788. Positions 24-677.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000014316.

Protein family/group databases

MEROPSM03.001.

Proteomic databases

PaxDbP47788.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397442.
KEGGssc:397442.

Organism-specific databases

CTD7064.

Phylogenomic databases

eggNOGCOG0339.
HOGENOMHOG000245985.
HOVERGENHBG000238.
KOK01392.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHOP1_PIG
AccessionPrimary (citable) accession number: P47788
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries