P47788 (THOP1_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thimet oligopeptidase
EC=3.4.24.15

Alternative name(s):
Endopeptidase 24.15

Gene names

Name:

THOP1

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	687 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation.
Catalytic activity	Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 687	687	Thimet oligopeptidase		PRO_0000078154
Sites
	Active site	474	1	By similarity
	Metal binding	473	1	Zinc; catalytic By similarity
	Metal binding	477	1	Zinc; catalytic By similarity
	Metal binding	480	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	278	1	Phosphotyrosine By similarity
	Modified residue	538	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47788 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 70AEF85C6C466929	FASTA	687	78,144
	10 20 30 40 50 60 MKPPAACAGD ALDVAAPCSA VNYLRWDLSA QQIGELTTEL IEQTKRVYDR VGTQELQDVS 70 80 90 100 110 120 YENTLKALAD VEVSYTVQRN ILDFPQHVSP CKDIRTASTE ADKKLSEFDV EMSMRQDVYQ 130 140 150 160 170 180 RIVWLQEKVQ KDSLRPEAAR YLERLIKLGR RNGLHLPKET QEKIKSIKKK LSLLCIDFNK 190 200 210 220 230 240 NLNEDTTFLP VTREELGGLP EDFLNSLEKT EDEKLKVTLK YPHYFPLLKK CHVPETRRKV 250 260 270 280 290 300 EEAFNCRCKE ENCAILRELV RLRAQKSSLL GFSTHADYVL EMNMAKTSQV VATFLDELAQ 310 320 330 340 350 360 KLKPLGEQER AVILELKKAE CTKRGLDFDG RINAWDMRYY MNQVEETRYR VDQNLLKEYF 370 380 390 400 410 420 PMQVVTRGLL GIYQELLGLT FHLEEGAAVW HEDVMLYSVR DAASGKVIGK FYLDLYPREG 430 440 450 460 470 480 KYGHAACFGL QPGCLRQDGS RQIAIAAMVA NFTKPTPDAP SLLQHDEVET YFHEFGHVMH 490 500 510 520 530 540 QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEAEPLLR MSQHYRTGSA IPQELLEKLI 550 560 570 580 590 600 KSRQANTGLF NLRQIVLAKV DQALHTQTAA DPAEEYARLC QEILGVPATP GTNMPATFGH 610 620 630 640 650 660 LAGGYDAQYY GYLWSEVYSA DMFHTRFKQE GILSGKVGMD YRSCILRPGG SEDASVMLKL 670 680 FLGRDPKQDA FLLSKGLQVE GCEPPAS

References

[1]	"Cloning, amino acid sequence and tissue distribution of porcine thimet oligopeptidase. A comparison with soluble angiotensin-binding protein." Kato A., Sugiura N., Hagiwara H., Hirose S. Eur. J. Biochem. 221:159-165(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Targeting of endopeptidase 24.16 to different subcellular compartments by alternative promoter usage." Kato A., Sugiura N., Saruta Y., Hosoiri T., Yasue H., Hirose S. J. Biol. Chem. 272:15313-15322(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D21871 mRNA. Translation: BAA04882.1. AB000438 Genomic DNA. Translation: BAA19107.1.
PIR	S43250.
RefSeq	NP_999388.1. NM_214223.2.
UniGene	Ssc.16161.
3D structure databases
ProteinModelPortal	P47788.
SMR	P47788. Positions 24-677.
ModBase	Search...
Protein family/group databases
MEROPS	M03.001.
Proteomic databases
PaxDb	P47788.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	397442.
KEGG	ssc:397442.
Organism-specific databases
CTD	7064.
Phylogenomic databases
eggNOG	COG0339.
HOGENOM	HOG000245985.
HOVERGEN	HBG000238.
KO	K01392.
OrthoDB	EOG4CG07P.
Family and domain databases
Gene3D	1.10.1370.10. 2 hits. 1.20.1050.40. 1 hit. 3.40.390.10. 1 hit.
InterPro	IPR024079. MetalloPept_cat_dom. IPR024077. Neurolysin/TOP_dom2. IPR024080. Neurolysin/TOP_N. IPR001567. Pept_M3A_M3B. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THOP1_PIG

Accession

Primary (citable) accession number: P47788

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program