Reviewed, UniProtKB/Swiss-Prot P47784 (RNAS2_PONPY)

Names and origin

Protein names

Recommended name:
    Non-secretory ribonuclease
    EC=3.1.27.5
Alternative name(s):
    Ribonuclease US
    Eosinophil-derived neurotoxin
    RNase UpI-2
    Ribonuclease 2
      Short name=RNase 2

Gene names

Name:	RNASE2
Synonyms:	EDN, RNS2

Organism

Pongo pygmaeus (Bornean orangutan)

Taxonomic identifier

9600 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subcellular location	Lysosome Probable. Cytoplasmic granule. Note: Matrix of eosinophil's large specific granule.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein Nitration
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 161	134	Non-secretory ribonuclease		PRO_0000030880
Regions
	Region	65 – 69	5	Substrate binding By similarity
Sites
	Active site	42	1	Proton acceptor By similarity
	Active site	156	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	34	1	C-linked (Man) By similarity
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Disulfide bond	50 ↔ 110		By similarity
	Disulfide bond	64 ↔ 123		By similarity
	Disulfide bond	82 ↔ 138		By similarity
	Disulfide bond	89 ↔ 98		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47784-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F237D6D1566750C5	FASTA	161	18,327
	10 20 30 40 50 60 MVPKLFTSQI SLLLLLGLLA VDGSLHVKPP QFTWAQWFET QHINMTSQQC NNAMQVINNF 70 80 90 100 110 120 QRRCKNQNTF LRTTFANVVN VCGNPNITCP SNRSRNNCHH SGVQVPLIHC NLTTPSPQNI 130 140 150 160 SNCRYAQTPA NMFYIVACDN RDPRRDPPQY PVVPVHLDRI I

References

[1]	"Rapid evolution of a unique family of primate ribonuclease genes." Rosenberg H.F., Dyer K.D., Tiffany H.L., Gonzalez M. Nat. Genet. 10:219-223(1995) [PubMed: 7663519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U24104 Genomic DNA. Translation: AAC50148.1.
PIR	I61897.
3D structure databases
HSSP	HSSP built from PDB template 1K2A based on UniProtKB P10153.
SMR	P47784. Positions 26-161.
ModBase	Search...
Genome annotation databases
Ensembl	ENSPPYT00000006602; ENSPPYP00000006348; ENSPPYG00000005580; Pongo pygmaeus. [Genome view]
Phylogenomic databases
HOVERGEN	P47784.
Enzyme and pathway databases
BRENDA	3.1.27.5. 269192.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS2_PONPY

Accession

Primary (citable) accession number: P47784

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	October 13, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)