P47783 (RNAS2_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-secretory ribonuclease
EC=3.1.27.5

Alternative name(s):
Eosinophil-derived neurotoxin
RNase UpI-2
Ribonuclease 2
Short name=RNase 2
Ribonuclease US

Gene names

Name:	RNASE2
Synonyms:	EDN, RNS2

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities. Ref.1
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates. Ref.1
Subunit structure	Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur By similarity.
Subcellular location	Lysosome Probable. Cytoplasmic granule. Note: Matrix of eosinophil's large specific granule.
Sequence similarities	Belongs to the pancreatic ribonuclease family.
Biophysicochemical properties	Kinetic parameters: KM=1.8 µM for yeast tRNA (in the presence of 40 mM sodium phosphate at pH 7.0) Ref.1

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein Nitration
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 160	133	Non-secretory ribonuclease		PRO_0000030876
Regions
	Region	65 – 69	5	Substrate binding By similarity
Sites
	Active site	42	1	Proton acceptor By similarity
	Active site	155	1	Proton donor By similarity
Amino acid modifications
	Modified residue	60	1	Nitrated tyrosine By similarity
	Glycosylation	34	1	C-linked (Man) By similarity
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Disulfide bond	50 ↔ 110		By similarity
	Disulfide bond	64 ↔ 122		By similarity
	Disulfide bond	82 ↔ 137		By similarity
	Disulfide bond	89 ↔ 98		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47783 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 918F7840BC40C6F3	FASTA	160	18,000
	10 20 30 40 50 60 MVPKLFTSQI CLLLLLGLMG VEGSLHAKPG QFTWAQWFEI QHINMTSGQC TNAMQVINNY 70 80 90 100 110 120 QRRCKNQNTF LLTTFADVVH VCGNPSMPCP SNTSLNNCHH SGVQVPLIHC NLTTPSRRIS 130 140 150 160 NCRYTQTTAN KYYIVACNNS DPVRDPPQYP VVPVHLDRII

References

[1]	"Rapid evolution of a unique family of primate ribonuclease genes." Rosenberg H.F., Dyer K.D., Tiffany H.L., Gonzalez M. Nat. Genet. 10:219-223(1995) [PubMed: 7663519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24096 Genomic DNA. Translation: AAC50145.1.
PIR	I84444.
3D structure databases
ProteinModelPortal	P47783.
SMR	P47783. Positions 26-160.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS2_MACFA

Accession

Primary (citable) accession number: P47783

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	July 27, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program