P47776 (HEP2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparin cofactor 2

Alternative name(s):
Heparin cofactor II
Short name=HC-II
Protease inhibitor leuserpin-2
Serpin D1

Gene names

Name:	SERPIND1
Synonyms:	HCF2

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT).
Domain	The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition By similarity.
Post-translational modification	N-glycosylated; different glycan composition appears to lead to two forms of this protein (56 and 60 kDa).
Sequence similarities	Belongs to the serpin family.

Ontologies

Keywords
Biological process	Blood coagulation
Domain	Repeat Signal
Ligand	Heparin-binding
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein Sulfation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 480	461	Heparin cofactor 2		PRO_0000032496
Regions
	Repeat	56 – 66	11	1
	Repeat	70 – 80	11	2
	Region	56 – 80	25	2 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like)
	Region	173 – 193	21	Glycosaminoglycan-binding site By similarity
Sites
	Site	444 – 445	2	Reactive bond By similarity
Amino acid modifications
	Modified residue	62	1	Sulfotyrosine By similarity
	Modified residue	75	1	Sulfotyrosine By similarity
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
	Glycosylation	368	1	N-linked (GlcNAc...) Potential
	Glycosylation	404	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P47776 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 803BCF25203CA4FA	FASTA	480	54,532
	10 20 30 40 50 60 MQHRPHLLLI SLTIMSVCGG SNGLTDQLNN KNLTMPLLPI EFHKENTVTN DWIPEGEEDD 70 80 90 100 110 120 DYLDLEKLLS EDDDYIDIID AVSPTDSEAS AGNILQLFQG KSRIQRLNIL NAKFAFSLYR 130 140 150 160 170 180 ALKDQANAFD NIFIAPVGIS TAMGMISLGL KGETHEQVHS VLHFRDFVNA SSKYEILTIH 190 200 210 220 230 240 NLFRKLTHRL FRRNFGYTLR SVNDLYVQKQ FPIREDFKAK VREYYFAEAQ AADFSDPAFI 250 260 270 280 290 300 SKANNHILKV TKGLIKEALE NVDPATQMMI LNCIYFKGTW VNKFPVEMTH NHNFRLNERE 310 320 330 340 350 360 VVKVSMMQTK GNFLAANDQE LACDVLQLEY VGGISMLIVV PHKLSGMKTL EAQLTPQVVE 370 380 390 400 410 420 RWQKSMTNRT REVLLPKFKL EKNYNLVEAL KSMGVTELFD KNGNMSGISD QGITMDLFKH 430 440 450 460 470 480 QGTITVNEEG TQAAAVTTVG FMPLSTQVRF TVDRPFLFLV YEHRTSCLLF MGKVANPVRS

References

[1]	"Molecular cloning and expression of rabbit heparin cofactor II: a plasma thrombin inhibitor highly conserved between species." Sheffield W.P., Schuyler P.D., Blajchman M.A. Thromb. Haemost. 71:778-782(1994) [PubMed: 7974347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S73493 mRNA. Translation: AAB32401.1.
PIR	I46990.
RefSeq	NP_001075798.1. NM_001082329.1.
UniGene	Ocu.3320.
3D structure databases
ProteinModelPortal	P47776.
SMR	P47776. Positions 56-480.
ModBase	Search...
Protein-protein interaction databases
STRING	P47776.
Protein family/group databases
MEROPS	I04.019.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009170.
Organism-specific databases
CTD	3053.
Phylogenomic databases
eggNOG	maNOG12700.
GeneTree	ENSGT00560000076649.
HOVERGEN	HBG101242.
OrthoDB	EOG4RV2R5.
Family and domain databases
InterPro	IPR000295. Prot_inh_Lserp2. IPR000215. Protease_inhib_I4_serpin. IPR023795. Protease_inhib_I4_serpin_CS. IPR023796. Sepin_dom. [Graphical view]
PANTHER	PTHR11461. Prot_inh_serpin. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
PRINTS	PR00780. LEUSERPINII.
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEP2_RABIT

Accession

Primary (citable) accession number: P47776

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program