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Protein

G-protein coupled receptor 12

Gene

GPR12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity). Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production.By similarity

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: ProtInc
  2. phosphatidylcholine binding Source: Ensembl

GO - Biological processi

  1. cellular calcium ion homeostasis Source: Ensembl
  2. G-protein coupled receptor signaling pathway Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 12
Gene namesi
Name:GPR12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 13

Organism-specific databases

HGNCiHGNC:4466. GPR12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848ExtracellularSequence AnalysisAdd
BLAST
Transmembranei49 – 6921Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini70 – 778CytoplasmicSequence Analysis
Transmembranei78 – 9821Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini99 – 11315ExtracellularSequence AnalysisAdd
BLAST
Transmembranei114 – 13421Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini135 – 15824CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei159 – 17921Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini180 – 19920ExtracellularSequence AnalysisAdd
BLAST
Transmembranei200 – 22021Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini221 – 25232CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei253 – 27321Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini274 – 2829ExtracellularSequence Analysis
Transmembranei283 – 30321Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini304 – 33431CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28856.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334G-protein coupled receptor 12PRO_0000069527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Lipidationi317 – 3171S-palmitoyl cysteineBy similarity
Modified residuei330 – 3301PhosphoserineSequence Analysis
Modified residuei332 – 3321PhosphoserineSequence Analysis

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP47775.
PRIDEiP47775.

Expressioni

Gene expression databases

BgeeiP47775.
CleanExiHS_GPR12.
ExpressionAtlasiP47775. baseline and differential.
GenevestigatoriP47775.

Organism-specific databases

HPAiHPA050972.

Interactioni

Protein-protein interaction databases

BioGridi109096. 2 interactions.
IntActiP47775. 1 interaction.
STRINGi9606.ENSP00000370844.

Structurei

3D structure databases

ProteinModelPortaliP47775.
SMRiP47775. Positions 46-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309471.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiP47775.
KOiK04315.
OMAiLTYNSER.
OrthoDBiEOG7966H0.
PhylomeDBiP47775.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000599. GPR12.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00650. GPR12ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEDLKVNLS GLPRDYLDAA AAENISAAVS SRVPAVEPEP ELVVNPWDIV
60 70 80 90 100
LCTSGTLISC ENAIVVLIIF HNPSLRAPMF LLIGSLALAD LLAGIGLITN
110 120 130 140 150
FVFAYLLQSE ATKLVTIGLI VASFSASVCS LLAITVDRYL SLYYALTYHS
160 170 180 190 200
ERTVTFTYVM LVMLWGTSIC LGLLPVMGWN CLRDESTCSV VRPLTKNNAA
210 220 230 240 250
ILSVSFLFMF ALMLQLYIQI CKIVMRHAHQ IALQHHFLAT SHYVTTRKGV
260 270 280 290 300
STLAIILGTF AACWMPFTLY SLIADYTYPS IYTYATLLPA TYNSIINPVI
310 320 330
YAFRNQEIQK ALCLICCGCI PSSLAQRARS PSDV
Length:334
Mass (Da):36,730
Last modified:February 1, 1996 - v1
Checksum:i618334A6582D744B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18548 Genomic DNA. Translation: AAA91630.1.
AL159978 Genomic DNA. Translation: CAI14692.1.
BC067448 mRNA. Translation: AAH67448.1.
BC067452 mRNA. Translation: AAH67452.1.
BC112144 mRNA. Translation: AAI12145.1.
BC112146 mRNA. Translation: AAI12147.1.
CCDSiCCDS9319.1.
RefSeqiNP_005279.1. NM_005288.3.
UniGeneiHs.123034.

Genome annotation databases

EnsembliENST00000381436; ENSP00000370844; ENSG00000132975.
ENST00000405846; ENSP00000384932; ENSG00000132975.
GeneIDi2835.
KEGGihsa:2835.
UCSCiuc010aal.3. human.

Polymorphism databases

DMDMi1346168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18548 Genomic DNA. Translation: AAA91630.1.
AL159978 Genomic DNA. Translation: CAI14692.1.
BC067448 mRNA. Translation: AAH67448.1.
BC067452 mRNA. Translation: AAH67452.1.
BC112144 mRNA. Translation: AAI12145.1.
BC112146 mRNA. Translation: AAI12147.1.
CCDSiCCDS9319.1.
RefSeqiNP_005279.1. NM_005288.3.
UniGeneiHs.123034.

3D structure databases

ProteinModelPortaliP47775.
SMRiP47775. Positions 46-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109096. 2 interactions.
IntActiP47775. 1 interaction.
STRINGi9606.ENSP00000370844.

Chemistry

ChEMBLiCHEMBL5006.
GuidetoPHARMACOLOGYi86.

Protein family/group databases

GPCRDBiSearch...

Polymorphism databases

DMDMi1346168.

Proteomic databases

PaxDbiP47775.
PRIDEiP47775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000381436; ENSP00000370844; ENSG00000132975.
ENST00000405846; ENSP00000384932; ENSG00000132975.
GeneIDi2835.
KEGGihsa:2835.
UCSCiuc010aal.3. human.

Organism-specific databases

CTDi2835.
GeneCardsiGC13M027331.
HGNCiHGNC:4466. GPR12.
HPAiHPA050972.
MIMi600752. gene.
neXtProtiNX_P47775.
PharmGKBiPA28856.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG309471.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiP47775.
KOiK04315.
OMAiLTYNSER.
OrthoDBiEOG7966H0.
PhylomeDBiP47775.
TreeFamiTF330052.

Miscellaneous databases

GeneWikiiGPR12.
GenomeRNAii2835.
NextBioi11189.
PROiP47775.
SOURCEiSearch...

Gene expression databases

BgeeiP47775.
CleanExiHS_GPR12.
ExpressionAtlasiP47775. baseline and differential.
GenevestigatoriP47775.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000599. GPR12.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00650. GPR12ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and chromosomal localization of human genes encoding three closely related G protein-coupled receptors."
    Song Z.-H., Modi W., Bonner T.I.
    Genomics 28:347-349(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.
  4. "Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G protein-coupled receptors."
    Uhlenbrock K., Gassenhuber J., Kostenis E.
    Cell. Signal. 14:941-953(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY FUNCTION.
  5. "Lipid G protein-coupled receptor ligand identification using beta-arrestin PathHunter assay."
    Yin H., Chu A., Li W., Wang B., Shelton F., Otero F., Nguyen D.G., Caldwell J.S., Chen Y.A.
    J. Biol. Chem. 284:12328-12338(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF FUNCTION AS A SPHINGOSINE 1-PHOSPHATE RECEPTOR.

Entry informationi

Entry nameiGPR12_HUMAN
AccessioniPrimary (citable) accession number: P47775
Secondary accession number(s): Q5T8P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 7, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally (PubMed:12220620) thought to be a receptor for sphingosine 1-phosphate. It has been demonstrated that it is not the case in human (PubMed:19286662).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.