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P47775 (GPR12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 12
Gene names
Name:GPR12
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes neurite outgrowth and blocks myelin inhibition in neurons By similarity. Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. Ref.4 Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Caution

Was originally (Ref.4) thought to be a receptor for sphingosine 1-phosphate. It has been demonstrated that it is not the case in human (Ref.5).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334G-protein coupled receptor 12
PRO_0000069527

Regions

Topological domain1 – 4848Extracellular Potential
Transmembrane49 – 6921Helical; Name=1; Potential
Topological domain70 – 778Cytoplasmic Potential
Transmembrane78 – 9821Helical; Name=2; Potential
Topological domain99 – 11315Extracellular Potential
Transmembrane114 – 13421Helical; Name=3; Potential
Topological domain135 – 15824Cytoplasmic Potential
Transmembrane159 – 17921Helical; Name=4; Potential
Topological domain180 – 19920Extracellular Potential
Transmembrane200 – 22021Helical; Name=5; Potential
Topological domain221 – 25232Cytoplasmic Potential
Transmembrane253 – 27321Helical; Name=6; Potential
Topological domain274 – 2829Extracellular Potential
Transmembrane283 – 30321Helical; Name=7; Potential
Topological domain304 – 33431Cytoplasmic Potential

Amino acid modifications

Modified residue3301Phosphoserine Potential
Modified residue3321Phosphoserine Potential
Lipidation3171S-palmitoyl cysteine By similarity
Glycosylation81N-linked (GlcNAc...) Potential
Glycosylation241N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P47775 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 618334A6582D744B

FASTA33436,730
        10         20         30         40         50         60 
MNEDLKVNLS GLPRDYLDAA AAENISAAVS SRVPAVEPEP ELVVNPWDIV LCTSGTLISC 

        70         80         90        100        110        120 
ENAIVVLIIF HNPSLRAPMF LLIGSLALAD LLAGIGLITN FVFAYLLQSE ATKLVTIGLI 

       130        140        150        160        170        180 
VASFSASVCS LLAITVDRYL SLYYALTYHS ERTVTFTYVM LVMLWGTSIC LGLLPVMGWN 

       190        200        210        220        230        240 
CLRDESTCSV VRPLTKNNAA ILSVSFLFMF ALMLQLYIQI CKIVMRHAHQ IALQHHFLAT 

       250        260        270        280        290        300 
SHYVTTRKGV STLAIILGTF AACWMPFTLY SLIADYTYPS IYTYATLLPA TYNSIINPVI 

       310        320        330 
YAFRNQEIQK ALCLICCGCI PSSLAQRARS PSDV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and chromosomal localization of human genes encoding three closely related G protein-coupled receptors."
Song Z.-H., Modi W., Bonner T.I.
Genomics 28:347-349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.
[4]"Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G protein-coupled receptors."
Uhlenbrock K., Gassenhuber J., Kostenis E.
Cell. Signal. 14:941-953(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY FUNCTION.
[5]"Lipid G protein-coupled receptor ligand identification using beta-arrestin PathHunter assay."
Yin H., Chu A., Li W., Wang B., Shelton F., Otero F., Nguyen D.G., Caldwell J.S., Chen Y.A.
J. Biol. Chem. 284:12328-12338(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: LACK OF FUNCTION AS A SPHINGOSINE 1-PHOSPHATE RECEPTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18548 Genomic DNA. Translation: AAA91630.1.
AL159978 Genomic DNA. Translation: CAI14692.1.
BC067448 mRNA. Translation: AAH67448.1.
BC067452 mRNA. Translation: AAH67452.1.
BC112144 mRNA. Translation: AAI12145.1.
BC112146 mRNA. Translation: AAI12147.1.
RefSeqNP_005279.1. NM_005288.3.
UniGeneHs.123034.

3D structure databases

ProteinModelPortalP47775.
SMRP47775. Positions 46-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109096. 2 interactions.
IntActP47775. 1 interaction.
STRING9606.ENSP00000370844.

Chemistry

ChEMBLCHEMBL5006.
GuidetoPHARMACOLOGY86.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM1346168.

Proteomic databases

PaxDbP47775.
PRIDEP47775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000381436; ENSP00000370844; ENSG00000132975.
ENST00000405846; ENSP00000384932; ENSG00000132975.
GeneID2835.
KEGGhsa:2835.
UCSCuc010aal.3. human.

Organism-specific databases

CTD2835.
GeneCardsGC13M027331.
HGNCHGNC:4466. GPR12.
HPAHPA050972.
MIM600752. gene.
neXtProtNX_P47775.
PharmGKBPA28856.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG309471.
HOGENOMHOG000294075.
HOVERGENHBG079561.
InParanoidP47775.
KOK04315.
OMALTYNSER.
OrthoDBEOG7966H0.
PhylomeDBP47775.
TreeFamTF330052.

Gene expression databases

ArrayExpressP47775.
BgeeP47775.
CleanExHS_GPR12.
GenevestigatorP47775.

Family and domain databases

InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000599. GPR12.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00650. GPR12ORPHANR.
PR00644. GPRORPHANR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR12.
GenomeRNAi2835.
NextBio11189.
PROP47775.
SOURCESearch...

Entry information

Entry nameGPR12_HUMAN
AccessionPrimary (citable) accession number: P47775
Secondary accession number(s): Q5T8P3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries