P47775 (GPR12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor 12

Gene names

Name:

GPR12

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes neurite outgrowth and blocks myelin inhibition in neurons By similarity. Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. Ref.4 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	Was originally (Ref.4) thought to be a receptor for sphingosine 1-phosphate. It has been demonstrated that it is not the case in human (Ref.5).

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.1. Source: ProtInc phosphatidylcholine binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	G-protein coupled receptor 12		PRO_0000069527
Regions
	Topological domain	1 – 48	48	Extracellular Potential
	Transmembrane	49 – 69	21	Helical; Name=1; Potential
	Topological domain	70 – 77	8	Cytoplasmic Potential
	Transmembrane	78 – 98	21	Helical; Name=2; Potential
	Topological domain	99 – 113	15	Extracellular Potential
	Transmembrane	114 – 134	21	Helical; Name=3; Potential
	Topological domain	135 – 158	24	Cytoplasmic Potential
	Transmembrane	159 – 179	21	Helical; Name=4; Potential
	Topological domain	180 – 199	20	Extracellular Potential
	Transmembrane	200 – 220	21	Helical; Name=5; Potential
	Topological domain	221 – 252	32	Cytoplasmic Potential
	Transmembrane	253 – 273	21	Helical; Name=6; Potential
	Topological domain	274 – 282	9	Extracellular Potential
	Transmembrane	283 – 303	21	Helical; Name=7; Potential
	Topological domain	304 – 334	31	Cytoplasmic Potential
Amino acid modifications
	Modified residue	330	1	Phosphoserine Potential
	Modified residue	332	1	Phosphoserine Potential
	Lipidation	317	1	S-palmitoyl cysteine By similarity
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P47775 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 618334A6582D744B	FASTA	334	36,730
	10 20 30 40 50 60 MNEDLKVNLS GLPRDYLDAA AAENISAAVS SRVPAVEPEP ELVVNPWDIV LCTSGTLISC 70 80 90 100 110 120 ENAIVVLIIF HNPSLRAPMF LLIGSLALAD LLAGIGLITN FVFAYLLQSE ATKLVTIGLI 130 140 150 160 170 180 VASFSASVCS LLAITVDRYL SLYYALTYHS ERTVTFTYVM LVMLWGTSIC LGLLPVMGWN 190 200 210 220 230 240 CLRDESTCSV VRPLTKNNAA ILSVSFLFMF ALMLQLYIQI CKIVMRHAHQ IALQHHFLAT 250 260 270 280 290 300 SHYVTTRKGV STLAIILGTF AACWMPFTLY SLIADYTYPS IYTYATLLPA TYNSIINPVI 310 320 330 YAFRNQEIQK ALCLICCGCI PSSLAQRARS PSDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and chromosomal localization of human genes encoding three closely related G protein-coupled receptors." Song Z.-H., Modi W., Bonner T.I. Genomics 28:347-349(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.
[4]	"Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G protein-coupled receptors." Uhlenbrock K., Gassenhuber J., Kostenis E. Cell. Signal. 14:941-953(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY FUNCTION.
[5]	"Lipid G protein-coupled receptor ligand identification using beta-arrestin PathHunter assay." Yin H., Chu A., Li W., Wang B., Shelton F., Otero F., Nguyen D.G., Caldwell J.S., Chen Y.A. J. Biol. Chem. 284:12328-12338(2009) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF FUNCTION AS A SPHINGOSINE 1-PHOSPHATE RECEPTOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18548 Genomic DNA. Translation: AAA91630.1. AL159978 Genomic DNA. Translation: CAI14692.1. BC067448 mRNA. Translation: AAH67448.1. BC067452 mRNA. Translation: AAH67452.1. BC112144 mRNA. Translation: AAI12145.1. BC112146 mRNA. Translation: AAI12147.1.
IPI	IPI00026228.
RefSeq	NP_005279.1. NM_005288.3.
UniGene	Hs.123034.
3D structure databases
ProteinModelPortal	P47775.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47775. 1 interaction.
STRING	9606.ENSP00000370844.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	1346168.
Proteomic databases
PaxDb	P47775.
PRIDE	P47775.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000381436; ENSP00000370844; ENSG00000132975. ENST00000405846; ENSP00000384932; ENSG00000132975.
GeneID	2835.
KEGG	hsa:2835.
UCSC	uc010aal.3. human.
Organism-specific databases
CTD	2835.
GeneCards	GC13M027331.
HGNC	HGNC:4466. GPR12.
HPA	HPA050972.
MIM	600752. gene.
neXtProt	NX_P47775.
PharmGKB	PA28856.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG309471.
HOGENOM	HOG000294075.
HOVERGEN	HBG079561.
InParanoid	P47775.
KO	K04315.
OMA	LTYNSER.
OrthoDB	EOG4MSD02.
PhylomeDB	P47775.
Gene expression databases
ArrayExpress	P47775.
Bgee	P47775.
CleanEx	HS_GPR12.
Genevestigator	P47775.
GermOnline	ENSG00000132975. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000599. GPR12. IPR000723. GPR_3/6/12_orphan. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00650. GPR12ORPHANR. PR00644. GPRORPHANR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5006.
GenomeRNAi	2835.
NextBio	11189.
SOURCE	Search...

Entry information

Entry name

GPR12_HUMAN

Accession

Primary (citable) accession number: P47775
Secondary accession number(s): Q5T8P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.