P47754 (CAZA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-actin-capping protein subunit alpha-2

Alternative name(s):
CapZ alpha-2

Gene names

Name:	Capza2
Synonyms:	Cappa2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Subunit structure	Heterodimer of an alpha and a beta subunit. Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH1, FAM21, KIAA1033, KIAA0196 and CCDC53. Interacts with RCSD1/CAPZIP By similarity.
Sequence similarities	Belongs to the F-actin-capping protein alpha subunit family.

Ontologies

Keywords
Ligand	Actin-binding
Molecular function	Actin capping
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro actin filament capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	F-actin capping protein complex Inferred from electronic annotation. Source: InterPro cortical cytoskeleton Inferred from direct assay. Source: MGI membrane Inferred from direct assay. Source: MGI
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 286	285	F-actin-capping protein subunit alpha-2		PRO_0000208628
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.3
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	86	1	N6-acetyllysine By similarity
	Modified residue	97	1	N6-acetyllysine By similarity
	Modified residue	273	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47754 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E706A9BC1830E70B	FASTA	286	32,967
	10 20 30 40 50 60 MADLEEQLSD EEKVRIAAKF IIHAPPGEFN EVFNDVRLLL NNDNLLREGA AHAFAQYNLD 70 80 90 100 110 120 QFTPVKIEGY EDQVLITEHG DLGNGKFLDP KNRICFKFDH LRKEATDPRP YEAENAIESW 130 140 150 160 170 180 RTSVETALRA YVKEHYPNGV CTVYGKKVDG QQTIIACIES HQFQAKNFWN GRWRSEWKFT 190 200 210 220 230 240 VTPSTTQVVG ILKIQVHYYE DGNVQLVSHK DIQDSLTVSN EVQTAKEFIK IVEAAENEYQ 250 260 270 280 TAISENYQTM SDTTFKALRR QLPVTRTKID WNKILSYKIG KEMQNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vertebrates have conserved capping protein alpha isoforms with specific expression patterns." Hart M.C., Korshunova Y.O., Cooper J.A. Cell Motil. Cytoskeleton 38:120-132(1997) [PubMed: 9331217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	Sumpton D.P., Sandilands E., Frame M.C., Bienvenut W.V. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-13; 20-86 AND 104-129, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
[4]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 20-37; 67-86 AND 194-210, MASS SPECTROMETRY. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16741 mRNA. Translation: AAC00567.1. BC082589 mRNA. Translation: AAH82589.1.
IPI	IPI00111265.
RefSeq	NP_031630.1. NM_007604.2.
UniGene	Mm.392504.
3D structure databases
ProteinModelPortal	P47754.
SMR	P47754. Positions 8-276.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-31382N.
IntAct	P47754. 8 interactions.
STRING	P47754.
PTM databases
PhosphoSite	P47754.
2D gel databases
REPRODUCTION-2DPAGE	P47754.
Proteomic databases
PRIDE	P47754.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000015877; ENSMUSP00000015877; ENSMUSG00000015733.
GeneID	12343.
KEGG	mmu:12343.
Organism-specific databases
CTD	830.
MGI	MGI:106222. Capza2.
Phylogenomic databases
eggNOG	roNOG04256.
HOGENOM	HBG397839.
HOVERGEN	HBG050810.
InParanoid	P47754.
OMA	AIESWRN.
OrthoDB	EOG45DWQ0.
PhylomeDB	P47754.
Gene expression databases
ArrayExpress	P47754.
Bgee	P47754.
Genevestigator	P47754.
GermOnline	ENSMUSG00000015733. Mus musculus.
Family and domain databases
InterPro	IPR017865. F-actin_cap_asu_CS. IPR002189. WASH_F-actin_cap_alpha. [Graphical view]
KO	K10364.
PANTHER	PTHR10653. F-actin_cap_A. 1 hit.
Pfam	PF01267. F-actin_cap_A. 1 hit. [Graphical view]
PRINTS	PR00191. FACTINCAPA.
PROSITE	PS00748. F_ACTIN_CAPPING_A_1. 1 hit. PS00749. F_ACTIN_CAPPING_A_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280972.
SOURCE	Search...

Entry information

Entry name

CAZA2_MOUSE

Accession

Primary (citable) accession number: P47754

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program