P47753 (CAZA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-actin-capping protein subunit alpha-1

Alternative name(s):
CapZ alpha-1

Gene names

Name:	Capza1
Synonyms:	Cappa1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Subunit structure	Heterodimer of an alpha and a beta subunit. Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH1, FAM21, KIAA1033, KIAA0196 and CCDC53. Interacts with S100B and S100A By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Sequence similarities	Belongs to the F-actin-capping protein alpha subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Molecular function	Actin capping
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro barbed-end actin filament capping Inferred from direct assay PubMed 17656356. Source: MGI
Cellular_component	F-actin capping protein complex Inferred from direct assay PubMed 11604420. Source: MGI WASH complex Inferred from sequence or structural similarity. Source: UniProtKB cortical cytoskeleton Inferred from direct assay PubMed 18723693. Source: MGI membrane Inferred from direct assay PubMed 18723693. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 286	285	F-actin-capping protein subunit alpha-1		PRO_0000208625
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	19	1	N6-acetyllysine By similarity
	Modified residue	97	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	222	1	V → I in AAC00566. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47753 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 6FE97BCE5D9B9D3A	FASTA	286	32,940
	10 20 30 40 50 60 MADFEDRVSD EEKVRIAAKF ITHAPPGEFN EVFNDVRLLL NNDNLLREGA AHAFAQYNMD 70 80 90 100 110 120 QFTPVKIEGY DDQVLITEHG DLGNSRFLDP RNQISFKFDH LRKEASDPQP EDVDGGLKSW 130 140 150 160 170 180 RESCDSALRA YVKDHYSNGF CTVYAKTIDG QQTIIACIES HQFQPKNFWN GRWRSEWKFT 190 200 210 220 230 240 ITPPSAQVVG VLKIQVHYYE DGNVQLVSHK DVQDSVTVSN EVQTTKEFIK IIESAENEYQ 250 260 270 280 TAISENYQTM SDTTFKALRR QLPVTRTKID WNKILSYKIG KEMQNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Vertebrates have conserved capping protein alpha isoforms with specific expression patterns." Hart M.C., Korshunova Y.O., Cooper J.A. Cell Motil. Cytoskeleton 38:120-132(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-286. Tissue: Muscle.
[3]	Lubec G., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 194-210. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC016232 mRNA. Translation: AAH16232.1. U16740 mRNA. Translation: AAC00566.1.
IPI	IPI00330063.
RefSeq	NP_033927.2. NM_009797.2.
UniGene	Mm.19142.
3D structure databases
ProteinModelPortal	P47753.
SMR	P47753. Positions 7-281.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47753. 22 interactions.
PTM databases
PhosphoSite	P47753.
2D gel databases
REPRODUCTION-2DPAGE	P47753.
Proteomic databases
PaxDb	P47753.
PRIDE	P47753.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	12340.
KEGG	mmu:12340.
Organism-specific databases
CTD	829.
MGI	MGI:106227. Capza1.
Phylogenomic databases
eggNOG	NOG261759.
HOVERGEN	HBG050810.
InParanoid	P47753.
KO	K10364.
OrthoDB	EOG45DWQ0.
Gene expression databases
Genevestigator	P47753.
GermOnline	ENSMUSG00000074348. Mus musculus.
Family and domain databases
InterPro	IPR017865. F-actin_cap_asu_CS. IPR002189. WASH_F-actin_cap_alpha. [Graphical view]
PANTHER	PTHR10653. PTHR10653. 1 hit.
Pfam	PF01267. F-actin_cap_A. 1 hit. [Graphical view]
PRINTS	PR00191. FACTINCAPA.
PROSITE	PS00748. F_ACTIN_CAPPING_A_1. 1 hit. PS00749. F_ACTIN_CAPPING_A_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CAPZA1. mouse.
NextBio	280968.
SOURCE	Search...

Entry information

Entry name

CAZA1_MOUSE

Accession

Primary (citable) accession number: P47753
Secondary accession number(s): Q91YN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 105 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program