Reviewed, UniProtKB/Swiss-Prot P47746 (CNR1_MOUSE)

Names and origin

Protein names

Recommended name:
    Cannabinoid receptor 1
      Short name=CB1
      Short name=CB-R
Alternative name(s):
    Brain-type cannabinoid receptor

Gene names

Name:

Cnr1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca2+ channel current.
Subunit structure	Interacts (via C-terminus) with CNRIP1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cannabinoid receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Cannabinoid receptor 1		PRO_0000069316
Regions
	Topological domain	1 – 117	117	Extracellular Potential
	Transmembrane	118 – 143	26	1 Potential
	Topological domain	144 – 155	12	Cytoplasmic Potential
	Transmembrane	156 – 176	21	2 Potential
	Topological domain	177 – 188	12	Extracellular Potential
	Transmembrane	189 – 213	25	3 Potential
	Topological domain	214 – 233	20	Cytoplasmic Potential
	Transmembrane	234 – 256	23	4 Potential
	Topological domain	257 – 274	18	Extracellular Potential
	Transmembrane	275 – 300	26	5 Potential
	Topological domain	301 – 345	45	Cytoplasmic Potential
	Transmembrane	346 – 366	21	6 Potential
	Topological domain	367 – 378	12	Extracellular Potential
	Transmembrane	379 – 400	22	7 Potential
	Topological domain	401 – 473	73	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	9	1	A → G in AAA57202. Ref.1
	Sequence conflict	115	1	S → R in AAA57202. Ref.1
	Sequence conflict	211	1	T → R in AAA57202. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47746-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E504168191CB6429	FASTA	473	52,831
	10 20 30 40 50 60 MKSILDGLAD TTFRTITTDL LYVGSNDIQY EDIKGDMASK LGYFPQKFPL TSFRGSPFQE 70 80 90 100 110 120 KMTAGDNSPL VPAGDTTNIT EFYNKSLSSF KENEDNIQCG ENFMDMECFM ILNPSQQLAI 130 140 150 160 170 180 AVLSLTLGTF TVLENLLVLC VILHSRSLRC RPSYHFIGSL AVADLLGSVI FVYSFVDFHV 190 200 210 220 230 240 FHRKDSPNVF LFKLGGVTAS FTASVGSLFL TAIDRYISIH RPLAYKRIVT RPKAVVAFCL 250 260 270 280 290 300 MWTIAIVIAV LPLLGWNCKK LQSVCSDIFP LIDETYLMFW IGVTSVLLLF IVYAYMYILW 310 320 330 340 350 360 KAHSHAVRMI QRGTQKSIII HTSEDGKVQV TRPDQARMDI RLAKTLVLIL VVLIICWGPL 370 380 390 400 410 420 LAIMVYDVFG KMNKLIKTVF AFCSMLCLLN STVNPIIYAL RSKDLRHAFR SMFPSCEGTA 430 440 450 460 470 QPLDNSMGDS DCLHKHANNT ASMHRAAESC IKSTVKIAKV TMSVSTDTSA EAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a cDNA encoding the mouse brain-type cannabinoid receptor protein." Chakrabarti A., Onaivi E.S., Chaudhuri G. DNA Seq. 5:385-388(1995) [PubMed: 8777318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Determination of the cannabinoid receptors in mouse x rat hybridoma NG108-15 cells and rat GH4C1 cells." Ho B.Y., Zhao J. Neurosci. Lett. 212:123-126(1996) [PubMed: 8832654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Unresponsiveness to cannabinoids and reduced addictive effects of opiates in CB1 receptor knockout mice." Ledent C., Valverde O., Cossu G., Petitet F., Aubert J.F., Beslot F., Boehme G.A., Imperato A., Pedrazzini T., Roques B.P., Vassart G., Fratta W., Parmentier M. Science 283:401-404(1999) [PubMed: 9888857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[4]	"Structure, expression and regulation of the cannabinoid receptor gene (CB1) in Huntington's disease transgenic mice." McCaw E.A., Hu H., Gomez G.T., Hebb A.L., Kelly M.E., Denovan-Wright E.M. Eur. J. Biochem. 271:4909-4920(2004) [PubMed: 15606779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: C57BL/6 X CBA.
[5]	Bonner T.I. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[6]	"cDNA cloning and expression analysis of mouse cannabinoid receptor (CB1) gene." Yuan Z.-Q., Li L., Qiu B.-S., Song D.-K. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Brain cortex.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U17985 mRNA. Translation: AAA57202.1. U40709 mRNA. Translation: AAA91176.1. Y18374 Genomic DNA. Translation: CAB42647.1. AY522555 Genomic DNA. Translation: AAS91801.1. AY522554 mRNA. Translation: AAS91800.1. U22948 Genomic DNA. Translation: AAA64413.1. AF153345 mRNA. Translation: AAD34624.1. AK139417 mRNA. Translation: BAE24003.1. BC070447 mRNA. Translation: AAH70447.1. BC079564 mRNA. Translation: AAH79564.1.
IPI	IPI00111245.
RefSeq	NP_031752.1.
UniGene	Mm.462047 Mm.7992
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P47746.
Proteomic databases
PRIDE	P47746.
Genome annotation databases
Ensembl	ENSMUSG00000044288. Mus musculus. [Contig view]
GeneID	12801.
KEGG	mmu:12801.
NMPDR	fig|10090.3.peg.9375.
Organism-specific databases
MGI	MGI:104615. Cnr1.
Phylogenomic databases
HOGENOM	P47746.
HOVERGEN	P47746.
OMA	P47746. FPLIDET.
Gene expression databases
ArrayExpress	P47746.
Bgee	P47746.
CleanEx	MM_CNR1.
GermOnline	ENSMUSG00000044288. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR002230. Cnbnoid_rcpt. IPR000810. Cnoid_rcpt_1. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR22750:SF10. Cnoid_rcpt_1. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PIRSF	PIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTS	PR00522. CANABINOID1R. PR00362. CANNABINOIDR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	282226.
SOURCE	Search...

Entry information

Entry name

CNR1_MOUSE

Accession

Primary (citable) accession number: P47746
Secondary accession number(s): Q5SF33

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)