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Protein

Cannabinoid receptor 1

Gene

Cnr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca2+ channel current.

GO - Molecular functioni

  1. cannabinoid receptor activity Source: UniProtKB
  2. drug binding Source: Ensembl

GO - Biological processi

  1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
  2. aging Source: Ensembl
  3. axonal fasciculation Source: MGI
  4. cannabinoid signaling pathway Source: MGI
  5. glucose homeostasis Source: MGI
  6. maternal process involved in female pregnancy Source: Ensembl
  7. memory Source: Ensembl
  8. negative regulation of action potential Source: Ensembl
  9. negative regulation of blood pressure Source: Ensembl
  10. negative regulation of dopamine secretion Source: Ensembl
  11. negative regulation of fatty acid beta-oxidation Source: Ensembl
  12. negative regulation of mast cell activation Source: Ensembl
  13. negative regulation of nitric-oxide synthase activity Source: Ensembl
  14. positive regulation of acute inflammatory response to antigenic stimulus Source: Ensembl
  15. positive regulation of apoptotic process Source: Ensembl
  16. positive regulation of blood pressure Source: Ensembl
  17. positive regulation of fever generation Source: Ensembl
  18. positive regulation of neuron projection development Source: Ensembl
  19. regulation of feeding behavior Source: Ensembl
  20. regulation of insulin secretion Source: Ensembl
  21. regulation of penile erection Source: Ensembl
  22. regulation of synaptic transmission, GABAergic Source: Ensembl
  23. regulation of synaptic transmission, glutamatergic Source: Ensembl
  24. response to cocaine Source: Ensembl
  25. response to ethanol Source: Ensembl
  26. response to lipopolysaccharide Source: Ensembl
  27. response to morphine Source: Ensembl
  28. response to nicotine Source: Ensembl
  29. response to nutrient Source: Ensembl
  30. sensory perception of pain Source: Ensembl
  31. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_250376. G alpha (i) signalling events.
REACT_261161. Class A/1 (Rhodopsin-like receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Cannabinoid receptor 1
Short name:
CB-R
Short name:
CB1
Alternative name(s):
Brain-type cannabinoid receptor
Gene namesi
Name:Cnr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:104615. Cnr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 117117ExtracellularSequence AnalysisAdd
BLAST
Transmembranei118 – 14326Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini144 – 15512CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei156 – 17621Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini177 – 18812ExtracellularSequence AnalysisAdd
BLAST
Transmembranei189 – 21325Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini214 – 23320CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei234 – 25623Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini257 – 27418ExtracellularSequence AnalysisAdd
BLAST
Transmembranei275 – 30026Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini301 – 34545CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei346 – 36621Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini367 – 37812ExtracellularSequence AnalysisAdd
BLAST
Transmembranei379 – 40022Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini401 – 47373CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axon Source: MGI
  2. growth cone Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: Ensembl
  5. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Cannabinoid receptor 1PRO_0000069316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Lipidationi416 – 4161S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylation at Cys-416 is important for recruitment at both plasma membrane and lipid rafts.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP47746.
PaxDbiP47746.
PRIDEiP47746.

PTM databases

PhosphoSiteiP47746.

Expressioni

Gene expression databases

BgeeiP47746.
CleanExiMM_CNR1.
ExpressionAtlasiP47746. baseline and differential.
GenevestigatoriP47746.

Interactioni

Subunit structurei

Interacts (via C-terminus) with CNRIP1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP47746.
SMRiP47746. Positions 117-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148018.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiP47746.
KOiK04277.
OMAiVAFCVMW.
OrthoDBiEOG7CK36K.
PhylomeDBiP47746.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000810. Canbinoid_rcpt_1.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR22750:SF10. PTHR22750:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTSiPR00522. CANABINOID1R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSILDGLAD TTFRTITTDL LYVGSNDIQY EDIKGDMASK LGYFPQKFPL
60 70 80 90 100
TSFRGSPFQE KMTAGDNSPL VPAGDTTNIT EFYNKSLSSF KENEDNIQCG
110 120 130 140 150
ENFMDMECFM ILNPSQQLAI AVLSLTLGTF TVLENLLVLC VILHSRSLRC
160 170 180 190 200
RPSYHFIGSL AVADLLGSVI FVYSFVDFHV FHRKDSPNVF LFKLGGVTAS
210 220 230 240 250
FTASVGSLFL TAIDRYISIH RPLAYKRIVT RPKAVVAFCL MWTIAIVIAV
260 270 280 290 300
LPLLGWNCKK LQSVCSDIFP LIDETYLMFW IGVTSVLLLF IVYAYMYILW
310 320 330 340 350
KAHSHAVRMI QRGTQKSIII HTSEDGKVQV TRPDQARMDI RLAKTLVLIL
360 370 380 390 400
VVLIICWGPL LAIMVYDVFG KMNKLIKTVF AFCSMLCLLN STVNPIIYAL
410 420 430 440 450
RSKDLRHAFR SMFPSCEGTA QPLDNSMGDS DCLHKHANNT ASMHRAAESC
460 470
IKSTVKIAKV TMSVSTDTSA EAL
Length:473
Mass (Da):52,831
Last modified:February 1, 1996 - v1
Checksum:iE504168191CB6429
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → G in AAA57202 (PubMed:8777318).Curated
Sequence conflicti115 – 1151S → R in AAA57202 (PubMed:8777318).Curated
Sequence conflicti211 – 2111T → R in AAA57202 (PubMed:8777318).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17985 mRNA. Translation: AAA57202.1.
U40709 mRNA. Translation: AAA91176.1.
Y18374 Genomic DNA. Translation: CAB42647.1.
AY522555 Genomic DNA. Translation: AAS91801.1.
AY522554 mRNA. Translation: AAS91800.1.
U22948 Genomic DNA. Translation: AAA64413.1.
AF153345 mRNA. Translation: AAD34624.1.
AK139417 mRNA. Translation: BAE24003.1.
BC070447 mRNA. Translation: AAH70447.1.
BC079564 mRNA. Translation: AAH79564.1.
CCDSiCCDS18025.1.
RefSeqiNP_031752.1. NM_007726.3.
XP_006537654.1. XM_006537591.1.
XP_006537655.1. XM_006537592.1.
XP_006537656.1. XM_006537593.1.
XP_006537657.1. XM_006537594.1.
XP_006537658.1. XM_006537595.1.
UniGeneiMm.7992.

Genome annotation databases

EnsembliENSMUST00000057188; ENSMUSP00000055797; ENSMUSG00000044288.
ENSMUST00000084736; ENSMUSP00000081787; ENSMUSG00000044288.
GeneIDi12801.
KEGGimmu:12801.
UCSCiuc008sfw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17985 mRNA. Translation: AAA57202.1.
U40709 mRNA. Translation: AAA91176.1.
Y18374 Genomic DNA. Translation: CAB42647.1.
AY522555 Genomic DNA. Translation: AAS91801.1.
AY522554 mRNA. Translation: AAS91800.1.
U22948 Genomic DNA. Translation: AAA64413.1.
AF153345 mRNA. Translation: AAD34624.1.
AK139417 mRNA. Translation: BAE24003.1.
BC070447 mRNA. Translation: AAH70447.1.
BC079564 mRNA. Translation: AAH79564.1.
CCDSiCCDS18025.1.
RefSeqiNP_031752.1. NM_007726.3.
XP_006537654.1. XM_006537591.1.
XP_006537655.1. XM_006537592.1.
XP_006537656.1. XM_006537593.1.
XP_006537657.1. XM_006537594.1.
XP_006537658.1. XM_006537595.1.
UniGeneiMm.7992.

3D structure databases

ProteinModelPortaliP47746.
SMRiP47746. Positions 117-417.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP47746.
ChEMBLiCHEMBL2111415.
GuidetoPHARMACOLOGYi56.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP47746.

Proteomic databases

MaxQBiP47746.
PaxDbiP47746.
PRIDEiP47746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057188; ENSMUSP00000055797; ENSMUSG00000044288.
ENSMUST00000084736; ENSMUSP00000081787; ENSMUSG00000044288.
GeneIDi12801.
KEGGimmu:12801.
UCSCiuc008sfw.1. mouse.

Organism-specific databases

CTDi1268.
MGIiMGI:104615. Cnr1.

Phylogenomic databases

eggNOGiNOG148018.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000049272.
HOVERGENiHBG051045.
InParanoidiP47746.
KOiK04277.
OMAiVAFCVMW.
OrthoDBiEOG7CK36K.
PhylomeDBiP47746.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiREACT_250376. G alpha (i) signalling events.
REACT_261161. Class A/1 (Rhodopsin-like receptors).

Miscellaneous databases

NextBioi282226.
PROiP47746.
SOURCEiSearch...

Gene expression databases

BgeeiP47746.
CleanExiMM_CNR1.
ExpressionAtlasiP47746. baseline and differential.
GenevestigatoriP47746.

Family and domain databases

InterProiIPR000810. Canbinoid_rcpt_1.
IPR002230. Cnbnoid_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR22750:SF10. PTHR22750:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTSiPR00522. CANABINOID1R.
PR00362. CANNABINOIDR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a cDNA encoding the mouse brain-type cannabinoid receptor protein."
    Chakrabarti A., Onaivi E.S., Chaudhuri G.
    DNA Seq. 5:385-388(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Determination of the cannabinoid receptors in mouse x rat hybridoma NG108-15 cells and rat GH4C1 cells."
    Ho B.Y., Zhao J.
    Neurosci. Lett. 212:123-126(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Unresponsiveness to cannabinoids and reduced addictive effects of opiates in CB1 receptor knockout mice."
    Ledent C., Valverde O., Cossu G., Petitet F., Aubert J.F., Beslot F., Boehme G.A., Imperato A., Pedrazzini T., Roques B.P., Vassart G., Fratta W., Parmentier M.
    Science 283:401-404(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  4. "Structure, expression and regulation of the cannabinoid receptor gene (CB1) in Huntington's disease transgenic mice."
    McCaw E.A., Hu H., Gomez G.T., Hebb A.L., Kelly M.E., Denovan-Wright E.M.
    Eur. J. Biochem. 271:4909-4920(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: C57BL/6 X CBA.
  5. Bonner T.I.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  6. "cDNA cloning and expression analysis of mouse cannabinoid receptor (CB1) gene."
    Yuan Z.-Q., Li L., Qiu B.-S., Song D.-K.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  7. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain cortex.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiCNR1_MOUSE
AccessioniPrimary (citable) accession number: P47746
Secondary accession number(s): Q5SF33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 4, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.