P47746 (CNR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cannabinoid receptor 1
Short name=CB-R
Short name=CB1

Alternative name(s):
Brain-type cannabinoid receptor

Gene names

Name:

Cnr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca2+ channel current.
Subunit structure	Interacts (via C-terminus) with CNRIP1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Palmitoylation at Cys-416 is important for recruitment at both plasma membrane and lipid rafts By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB aging Inferred from electronic annotation. Source: Compara maternal process involved in female pregnancy Inferred from electronic annotation. Source: Compara memory Inferred from electronic annotation. Source: Compara negative regulation of action potential Inferred from electronic annotation. Source: Compara negative regulation of blood pressure Inferred from electronic annotation. Source: Compara negative regulation of dopamine secretion Inferred from electronic annotation. Source: Compara negative regulation of fatty acid beta-oxidation Inferred from electronic annotation. Source: Compara negative regulation of mast cell activation Inferred from electronic annotation. Source: Compara negative regulation of nitric-oxide synthase activity Inferred from electronic annotation. Source: Compara positive regulation of acute inflammatory response to antigenic stimulus Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of blood pressure Inferred from electronic annotation. Source: Compara positive regulation of fever generation Inferred from electronic annotation. Source: Compara positive regulation of neuron projection development Inferred from electronic annotation. Source: Compara regulation of feeding behavior Inferred from electronic annotation. Source: Compara regulation of insulin secretion Inferred from electronic annotation. Source: Compara regulation of penile erection Inferred from electronic annotation. Source: Compara regulation of synaptic transmission, GABAergic Inferred from electronic annotation. Source: Compara regulation of synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara response to cocaine Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to morphine Inferred from electronic annotation. Source: Compara response to nicotine Inferred from electronic annotation. Source: Compara response to nutrient Inferred from electronic annotation. Source: Compara response to stress Inferred from electronic annotation. Source: Compara sensory perception of pain Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cannabinoid receptor activity Inferred from sequence or structural similarity. Source: UniProtKB drug binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Cannabinoid receptor 1		PRO_0000069316
Regions
	Topological domain	1 – 117	117	Extracellular Potential
	Transmembrane	118 – 143	26	Helical; Name=1; Potential
	Topological domain	144 – 155	12	Cytoplasmic Potential
	Transmembrane	156 – 176	21	Helical; Name=2; Potential
	Topological domain	177 – 188	12	Extracellular Potential
	Transmembrane	189 – 213	25	Helical; Name=3; Potential
	Topological domain	214 – 233	20	Cytoplasmic Potential
	Transmembrane	234 – 256	23	Helical; Name=4; Potential
	Topological domain	257 – 274	18	Extracellular Potential
	Transmembrane	275 – 300	26	Helical; Name=5; Potential
	Topological domain	301 – 345	45	Cytoplasmic Potential
	Transmembrane	346 – 366	21	Helical; Name=6; Potential
	Topological domain	367 – 378	12	Extracellular Potential
	Transmembrane	379 – 400	22	Helical; Name=7; Potential
	Topological domain	401 – 473	73	Cytoplasmic Potential
Amino acid modifications
	Lipidation	416	1	S-palmitoyl cysteine By similarity
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	9	1	A → G in AAA57202. Ref.1
	Sequence conflict	115	1	S → R in AAA57202. Ref.1
	Sequence conflict	211	1	T → R in AAA57202. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47746 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E504168191CB6429	FASTA	473	52,831
	10 20 30 40 50 60 MKSILDGLAD TTFRTITTDL LYVGSNDIQY EDIKGDMASK LGYFPQKFPL TSFRGSPFQE 70 80 90 100 110 120 KMTAGDNSPL VPAGDTTNIT EFYNKSLSSF KENEDNIQCG ENFMDMECFM ILNPSQQLAI 130 140 150 160 170 180 AVLSLTLGTF TVLENLLVLC VILHSRSLRC RPSYHFIGSL AVADLLGSVI FVYSFVDFHV 190 200 210 220 230 240 FHRKDSPNVF LFKLGGVTAS FTASVGSLFL TAIDRYISIH RPLAYKRIVT RPKAVVAFCL 250 260 270 280 290 300 MWTIAIVIAV LPLLGWNCKK LQSVCSDIFP LIDETYLMFW IGVTSVLLLF IVYAYMYILW 310 320 330 340 350 360 KAHSHAVRMI QRGTQKSIII HTSEDGKVQV TRPDQARMDI RLAKTLVLIL VVLIICWGPL 370 380 390 400 410 420 LAIMVYDVFG KMNKLIKTVF AFCSMLCLLN STVNPIIYAL RSKDLRHAFR SMFPSCEGTA 430 440 450 460 470 QPLDNSMGDS DCLHKHANNT ASMHRAAESC IKSTVKIAKV TMSVSTDTSA EAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a cDNA encoding the mouse brain-type cannabinoid receptor protein." Chakrabarti A., Onaivi E.S., Chaudhuri G. DNA Seq. 5:385-388(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Determination of the cannabinoid receptors in mouse x rat hybridoma NG108-15 cells and rat GH4C1 cells." Ho B.Y., Zhao J. Neurosci. Lett. 212:123-126(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Unresponsiveness to cannabinoids and reduced addictive effects of opiates in CB1 receptor knockout mice." Ledent C., Valverde O., Cossu G., Petitet F., Aubert J.F., Beslot F., Boehme G.A., Imperato A., Pedrazzini T., Roques B.P., Vassart G., Fratta W., Parmentier M. Science 283:401-404(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[4]	"Structure, expression and regulation of the cannabinoid receptor gene (CB1) in Huntington's disease transgenic mice." McCaw E.A., Hu H., Gomez G.T., Hebb A.L., Kelly M.E., Denovan-Wright E.M. Eur. J. Biochem. 271:4909-4920(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: C57BL/6 X CBA.
[5]	Bonner T.I. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[6]	"cDNA cloning and expression analysis of mouse cannabinoid receptor (CB1) gene." Yuan Z.-Q., Li L., Qiu B.-S., Song D.-K. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Brain cortex.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17985 mRNA. Translation: AAA57202.1. U40709 mRNA. Translation: AAA91176.1. Y18374 Genomic DNA. Translation: CAB42647.1. AY522555 Genomic DNA. Translation: AAS91801.1. AY522554 mRNA. Translation: AAS91800.1. U22948 Genomic DNA. Translation: AAA64413.1. AF153345 mRNA. Translation: AAD34624.1. AK139417 mRNA. Translation: BAE24003.1. BC070447 mRNA. Translation: AAH70447.1. BC079564 mRNA. Translation: AAH79564.1.
IPI	IPI00111245.
RefSeq	NP_031752.1. NM_007726.3.
UniGene	Mm.7992.
3D structure databases
ProteinModelPortal	P47746.
SMR	P47746. Positions 117-417.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P47746.
Proteomic databases
PaxDb	P47746.
PRIDE	P47746.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000057188; ENSMUSP00000055797; ENSMUSG00000044288. ENSMUST00000084736; ENSMUSP00000081787; ENSMUSG00000044288.
GeneID	12801.
KEGG	mmu:12801.
Organism-specific databases
CTD	1268.
MGI	MGI:104615. Cnr1.
Phylogenomic databases
eggNOG	NOG148018.
GeneTree	ENSGT00700000104088.
HOGENOM	HOG000049272.
HOVERGEN	HBG051045.
InParanoid	P47746.
KO	K04277.
OMA	ECFMILN.
OrthoDB	EOG41ZF9S.
Gene expression databases
ArrayExpress	P47746.
Bgee	P47746.
CleanEx	MM_CNR1.
Genevestigator	P47746.
GermOnline	ENSMUSG00000044288. Mus musculus.
Family and domain databases
InterPro	IPR002230. Cnbnoid_rcpt. IPR000810. Cnoid_rcpt_1. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
PANTHER	PTHR22750:SF10. PTHR22750:SF10. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PIRSF	PIRSF037995. Cnoid_rcpt_1. 1 hit.
PRINTS	PR00522. CANABINOID1R. PR00362. CANNABINOIDR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P47746.
ChEMBL	CHEMBL3037.
NextBio	282226.
SOURCE	Search...

Entry information

Entry name

CNR1_MOUSE

Accession

Primary (citable) accession number: P47746
Secondary accession number(s): Q5SF33

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program