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Protein

Metabotropic glutamate receptor 8

Gene

Grm8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei156 – 1561GlutamateBy similarity
Binding sitei227 – 2271GlutamateBy similarity
Binding sitei309 – 3091GlutamateBy similarity
Binding sitei401 – 4011GlutamateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Olfaction, Sensory transduction

Enzyme and pathway databases

ReactomeiR-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 8
Short name:
mGluR8
Gene namesi
Name:Grm8
Synonyms:Gprc1h, Mglur8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1351345. Grm8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 583550ExtracellularSequence analysisAdd
BLAST
Transmembranei584 – 60825Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini609 – 62012CytoplasmicSequence analysisAdd
BLAST
Transmembranei621 – 64121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini642 – 6476ExtracellularSequence analysis
Transmembranei648 – 66821Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini669 – 69527CytoplasmicSequence analysisAdd
BLAST
Transmembranei696 – 71621Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini717 – 74630ExtracellularSequence analysisAdd
BLAST
Transmembranei747 – 76822Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini769 – 78113CytoplasmicSequence analysisAdd
BLAST
Transmembranei782 – 80322Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini804 – 81815ExtracellularSequence analysisAdd
BLAST
Transmembranei819 – 84325Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini844 – 90865CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi868 – 8681K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-872 and R-882. 1 Publication
Mutagenesisi872 – 8721K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-868 and R-882. 1 Publication
Mutagenesisi882 – 8821K → R: Abolishes sumoylation. Abolishes sumoylation; when associated with R-868 and R-872. 1 Publication

Chemistry

ChEMBLiCHEMBL4626.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence analysisAdd
BLAST
Chaini34 – 908875Metabotropic glutamate receptor 8PRO_0000012942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 106By similarity
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence analysis
Disulfide bondi246 ↔ 534By similarity
Glycosylationi298 – 2981N-linked (GlcNAc...)Sequence analysis
Disulfide bondi369 ↔ 384By similarity
Disulfide bondi424 ↔ 431By similarity
Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence analysis
Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence analysis
Disulfide bondi516 ↔ 535By similarity
Disulfide bondi520 ↔ 538By similarity
Disulfide bondi541 ↔ 553By similarity
Disulfide bondi556 ↔ 569By similarity
Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence analysis
Cross-linki882 – 882Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP47743.
PaxDbiP47743.
PRIDEiP47743.

PTM databases

iPTMnetiP47743.
PhosphoSiteiP47743.

Expressioni

Tissue specificityi

Strongly expressed in olfactory bulb, accessory olfactory bulb, and mammillary body. Weaker expression in the retina, and in scattered cells in the cortex and hindbrain.

Gene expression databases

BgeeiP47743.
CleanExiMM_GRM8.
ExpressionAtlasiP47743. baseline and differential.
GenevisibleiP47743. MM.

Interactioni

Subunit structurei

Interacts with PICK1.By similarity

Protein-protein interaction databases

BioGridi200074. 5 interactions.
IntActiP47743. 1 interaction.
STRINGi10090.ENSMUSP00000110978.

Chemistry

BindingDBiP47743.

Structurei

3D structure databases

ProteinModelPortaliP47743.
SMRiP47743. Positions 38-849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni177 – 1793Glutamate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP47743.
KOiK04608.
OMAiRTHGQEY.
OrthoDBiEOG7Z0JXG.
PhylomeDBiP47743.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000144. GPCR_3_mtglu_rcpt_8.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR24060:SF26. PTHR24060:SF26. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01058. MTABOTROPC8R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCEGKRSTS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RLDGDIILGG
60 70 80 90 100
LFPVHAKGER GVPCGDLKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV
110 120 130 140 150
RILDTCSRDT YALEQSLTFV QALIEKDASD VKCANGDPPI FTKPDKISGV
160 170 180 190 200
IGAAASSVSI MVANILRLFK IPQISYASTA PELSDNTRYD FFSRVVPPDS
210 220 230 240 250
YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR EIGGVCIAQS
260 270 280 290 300
QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS
310 320 330 340 350
GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL
360 370 380 390 400
ANNRRNVWFA EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG
410 420 430 440 450
KVQFVIDAVY SMAYALHNMH KELCPGYIGL CPRMVTIDGK ELLGYIRAVN
460 470 480 490 500
FNGSAGTPVT FNENGDAPGR YDIFQYQINN KSTEYKIIGH WTNQLHLKVE
510 520 530 540 550
DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER CEGYNYQVDE
560 570 580 590 600
LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF
610 620 630 640 650
VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF
660 670 680 690 700
RRIFLGLGMC FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF
710 720 730 740 750
SLISVQLLGV FVWFVVDPPH TIIDYGEQRT LDPENARGVL KCDISDLSLI
760 770 780 790 800
CSLGYSILLM VTCTVYAIKT RGVPETFNEA KPIGFTMYTT CIIWLAFIPI
810 820 830 840 850
FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI IIFHPEQNVQ
860 870 880 890 900
KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY

ISYSNHSI
Length:908
Mass (Da):101,828
Last modified:September 27, 2005 - v2
Checksum:iE9952B6D356EB8A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861I → T in AAA68149 (PubMed:7722646).Curated
Sequence conflicti289 – 2891R → G in AAA68149 (PubMed:7722646).Curated
Sequence conflicti363 – 3653WEE → SEG in AAA68149 (PubMed:7722646).Curated
Sequence conflicti371 – 3711L → S in AAA68149 (PubMed:7722646).Curated
Sequence conflicti539 – 5391E → G in AAA68149 (PubMed:7722646).Curated
Sequence conflicti589 – 5891F → L in AAA68149 (PubMed:7722646).Curated
Sequence conflicti905 – 9051N → D in AAA68149 (PubMed:7722646).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17252 mRNA. Translation: AAA68149.1.
AY673682 mRNA. Translation: AAT76980.1.
CCDSiCCDS80495.1.
PIRiI49142.
RefSeqiNP_001298001.1. NM_001311072.1.
UniGeneiMm.320732.

Genome annotation databases

EnsembliENSMUST00000090512; ENSMUSP00000087998; ENSMUSG00000024211.
GeneIDi14823.
KEGGimmu:14823.
UCSCiuc009bcj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17252 mRNA. Translation: AAA68149.1.
AY673682 mRNA. Translation: AAT76980.1.
CCDSiCCDS80495.1.
PIRiI49142.
RefSeqiNP_001298001.1. NM_001311072.1.
UniGeneiMm.320732.

3D structure databases

ProteinModelPortaliP47743.
SMRiP47743. Positions 38-849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200074. 5 interactions.
IntActiP47743. 1 interaction.
STRINGi10090.ENSMUSP00000110978.

Chemistry

BindingDBiP47743.
ChEMBLiCHEMBL4626.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP47743.
PhosphoSiteiP47743.

Proteomic databases

MaxQBiP47743.
PaxDbiP47743.
PRIDEiP47743.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090512; ENSMUSP00000087998; ENSMUSG00000024211.
GeneIDi14823.
KEGGimmu:14823.
UCSCiuc009bcj.1. mouse.

Organism-specific databases

CTDi2918.
MGIiMGI:1351345. Grm8.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP47743.
KOiK04608.
OMAiRTHGQEY.
OrthoDBiEOG7Z0JXG.
PhylomeDBiP47743.

Enzyme and pathway databases

ReactomeiR-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

PROiP47743.
SOURCEiSearch...

Gene expression databases

BgeeiP47743.
CleanExiMM_GRM8.
ExpressionAtlasiP47743. baseline and differential.
GenevisibleiP47743. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000144. GPCR_3_mtglu_rcpt_8.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR24060:SF26. PTHR24060:SF26. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01058. MTABOTROPC8R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel metabotropic glutamate receptor expressed in the retina and olfactory bulb."
    Duvoisin R.M., Zhang C., Ramonell K.
    J. Neurosci. 15:3075-3083(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Retina.
  2. "Coupling of metabotropic glutamate receptor 8 to N-type Ca2+ channels in rat sympathetic neurons."
    Guo J., Ikeda S.R.
    Mol. Pharmacol. 67:1840-1851(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Brain.
  3. "Pias1 interaction and sumoylation of metabotropic glutamate receptor 8."
    Tang Z., El Far O., Betz H., Scheschonka A.
    J. Biol. Chem. 280:38153-38159(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION AT LYS-882, MUTAGENESIS OF LYS-868; LYS-872 AND LYS-882.

Entry informationi

Entry nameiGRM8_MOUSE
AccessioniPrimary (citable) accession number: P47743
Secondary accession number(s): Q6B964
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 27, 2005
Last modified: May 11, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.