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Protein

Metabotropic glutamate receptor 8

Gene

Grm8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156GlutamateBy similarity1
Binding sitei227GlutamateBy similarity1
Binding sitei309GlutamateBy similarity1
Binding sitei401GlutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processSensory transduction

Enzyme and pathway databases

ReactomeiR-MMU-418594 G alpha (i) signalling events
R-MMU-420499 Class C/3 (Metabotropic glutamate/pheromone receptors)

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 8
Short name:
mGluR8
Gene namesi
Name:Grm8
Synonyms:Gprc1h, Mglur8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1351345 Grm8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 583ExtracellularSequence analysisAdd BLAST550
Transmembranei584 – 608Helical; Name=1Sequence analysisAdd BLAST25
Topological domaini609 – 620CytoplasmicSequence analysisAdd BLAST12
Transmembranei621 – 641Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini642 – 647ExtracellularSequence analysis6
Transmembranei648 – 668Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini669 – 695CytoplasmicSequence analysisAdd BLAST27
Transmembranei696 – 716Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini717 – 746ExtracellularSequence analysisAdd BLAST30
Transmembranei747 – 768Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini769 – 781CytoplasmicSequence analysisAdd BLAST13
Transmembranei782 – 803Helical; Name=6Sequence analysisAdd BLAST22
Topological domaini804 – 818ExtracellularSequence analysisAdd BLAST15
Transmembranei819 – 843Helical; Name=7Sequence analysisAdd BLAST25
Topological domaini844 – 908CytoplasmicSequence analysisAdd BLAST65

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi868K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-872 and R-882. 1 Publication1
Mutagenesisi872K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-868 and R-882. 1 Publication1
Mutagenesisi882K → R: Abolishes sumoylation. Abolishes sumoylation; when associated with R-868 and R-872. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL4626

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000001294234 – 908Metabotropic glutamate receptor 8Add BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi64 ↔ 106By similarity
Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi246 ↔ 534By similarity
Glycosylationi298N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi369 ↔ 384By similarity
Disulfide bondi424 ↔ 431By similarity
Glycosylationi452N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi480N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi516 ↔ 535By similarity
Disulfide bondi520 ↔ 538By similarity
Disulfide bondi541 ↔ 553By similarity
Disulfide bondi556 ↔ 569By similarity
Glycosylationi565N-linked (GlcNAc...) asparagineSequence analysis1
Cross-linki882Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP47743
PRIDEiP47743

PTM databases

iPTMnetiP47743
PhosphoSitePlusiP47743

Expressioni

Tissue specificityi

Strongly expressed in olfactory bulb, accessory olfactory bulb, and mammillary body. Weaker expression in the retina, and in scattered cells in the cortex and hindbrain.

Gene expression databases

BgeeiENSMUSG00000024211
CleanExiMM_GRM8
ExpressionAtlasiP47743 baseline and differential
GenevisibleiP47743 MM

Interactioni

Subunit structurei

Interacts with PICK1.By similarity

Protein-protein interaction databases

BioGridi200074, 5 interactors
IntActiP47743, 1 interactor
MINTiP47743
STRINGi10090.ENSMUSP00000110978

Chemistry databases

BindingDBiP47743

Structurei

3D structure databases

ProteinModelPortaliP47743
SMRiP47743
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni177 – 179Glutamate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056 Eukaryota
ENOG410XR6W LUCA
GeneTreeiENSGT00760000118884
HOGENOMiHOG000218635
HOVERGENiHBG107965
InParanoidiP47743
KOiK04608
OMAiVDFPMVK
PhylomeDBiP47743

Family and domain databases

Gene3Di2.10.50.30, 1 hit
InterProiView protein in InterPro
IPR001828 ANF_lig-bd_rcpt
IPR000337 GPCR_3
IPR011500 GPCR_3_9-Cys_dom
IPR038550 GPCR_3_9-Cys_sf
IPR017978 GPCR_3_C
IPR017979 GPCR_3_CS
IPR000162 GPCR_3_mtglu_rcpt
IPR000144 GPCR_3_mtglu_rcpt_8
IPR028082 Peripla_BP_I
PfamiView protein in Pfam
PF00003 7tm_3, 1 hit
PF01094 ANF_receptor, 1 hit
PF07562 NCD3G, 1 hit
PRINTSiPR00248 GPCRMGR
PR01058 MTABOTROPC8R
PR00593 MTABOTROPICR
SUPFAMiSSF53822 SSF53822, 1 hit
PROSITEiView protein in PROSITE
PS00979 G_PROTEIN_RECEP_F3_1, 1 hit
PS00980 G_PROTEIN_RECEP_F3_2, 1 hit
PS00981 G_PROTEIN_RECEP_F3_3, 1 hit
PS50259 G_PROTEIN_RECEP_F3_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCEGKRSTS CPCFFLLTAK FYWILTMMQR THSQEYAHSI RLDGDIILGG
60 70 80 90 100
LFPVHAKGER GVPCGDLKKE KGIHRLEAML YAIDQINKDP DLLSNITLGV
110 120 130 140 150
RILDTCSRDT YALEQSLTFV QALIEKDASD VKCANGDPPI FTKPDKISGV
160 170 180 190 200
IGAAASSVSI MVANILRLFK IPQISYASTA PELSDNTRYD FFSRVVPPDS
210 220 230 240 250
YQAQAMVDIV TALGWNYVST LASEGNYGES GVEAFTQISR EIGGVCIAQS
260 270 280 290 300
QKIPREPRPG EFEKIIKRLL ETPNARAVIM FANEDDIRRI LEAAKKLNQS
310 320 330 340 350
GHFLWIGSDS WGSKIAPVYQ QEEIAEGAVT ILPKRASIDG FDRYFRSRTL
360 370 380 390 400
ANNRRNVWFA EFWEENFGCK LGSHGKRNSH IKKCTGLERI ARDSSYEQEG
410 420 430 440 450
KVQFVIDAVY SMAYALHNMH KELCPGYIGL CPRMVTIDGK ELLGYIRAVN
460 470 480 490 500
FNGSAGTPVT FNENGDAPGR YDIFQYQINN KSTEYKIIGH WTNQLHLKVE
510 520 530 540 550
DMQWANREHT HPASVCSLPC KPGERKKTVK GVPCCWHCER CEGYNYQVDE
560 570 580 590 600
LSCELCPLDQ RPNINRTGCQ RIPIIKLEWH SPWAVVPVFI AILGIIATTF
610 620 630 640 650
VIVTFVRYND TPIVRASGRE LSYVLLTGIF LCYSITFLMI AAPDTIICSF
660 670 680 690 700
RRIFLGLGMC FSYAALLTKT NRIHRIFEQG KKSVTAPKFI SPASQLVITF
710 720 730 740 750
SLISVQLLGV FVWFVVDPPH TIIDYGEQRT LDPENARGVL KCDISDLSLI
760 770 780 790 800
CSLGYSILLM VTCTVYAIKT RGVPETFNEA KPIGFTMYTT CIIWLAFIPI
810 820 830 840 850
FFGTAQSAEK MYIQTTTLTV SMSLSASVSL GMLYMPKVYI IIFHPEQNVQ
860 870 880 890 900
KRKRSFKAVV TAATMQSKLI QKGNDRPNGE VKSELCESLE TNTSSTKTTY

ISYSNHSI
Length:908
Mass (Da):101,828
Last modified:September 27, 2005 - v2
Checksum:iE9952B6D356EB8A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86I → T in AAA68149 (PubMed:7722646).Curated1
Sequence conflicti289R → G in AAA68149 (PubMed:7722646).Curated1
Sequence conflicti363 – 365WEE → SEG in AAA68149 (PubMed:7722646).Curated3
Sequence conflicti371L → S in AAA68149 (PubMed:7722646).Curated1
Sequence conflicti539E → G in AAA68149 (PubMed:7722646).Curated1
Sequence conflicti589F → L in AAA68149 (PubMed:7722646).Curated1
Sequence conflicti905N → D in AAA68149 (PubMed:7722646).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17252 mRNA Translation: AAA68149.1
AY673682 mRNA Translation: AAT76980.1
CCDSiCCDS80495.1
PIRiI49142
RefSeqiNP_001298001.1, NM_001311072.1
XP_017176886.1, XM_017321397.1
UniGeneiMm.320732

Genome annotation databases

EnsembliENSMUST00000090512; ENSMUSP00000087998; ENSMUSG00000024211
GeneIDi14823
KEGGimmu:14823
UCSCiuc009bcj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGRM8_MOUSE
AccessioniPrimary (citable) accession number: P47743
Secondary accession number(s): Q6B964
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 27, 2005
Last modified: March 28, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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