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Protein

Gamma-aminobutyric acid receptor subunit rho-2

Gene

Gabrr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Rho-2 GABA receptor could play a role in retinal neurotransmission.

GO - Molecular functioni

  • chloride channel activity Source: RGD
  • GABA-A receptor activity Source: RGD
  • inhibitory extracellular ligand-gated ion channel activity Source: RGD
  • protein domain specific binding Source: RGD

GO - Biological processi

  • chloride transmembrane transport Source: GOC
  • gamma-aminobutyric acid signaling pathway Source: Ensembl
  • ion transmembrane transport Source: GOC
  • visual perception Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977442. GABA A (rho) receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit rho-2
Alternative name(s):
GABA(A) receptor subunit rho-2
GABA(C) receptor
Gene namesi
Name:Gabrr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61902. Gabrr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 260240ExtracellularCuratedAdd
BLAST
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Transmembranei294 – 31421HelicalSequence analysisAdd
BLAST
Transmembranei326 – 34621HelicalSequence analysisAdd
BLAST
Topological domaini347 – 44397CytoplasmicCuratedAdd
BLAST
Transmembranei444 – 46421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 465445Gamma-aminobutyric acid receptor subunit rho-2PRO_0000000490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence analysis
Disulfide bondi178 ↔ 192By similarity
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP47742.
PRIDEiP47742.

PTM databases

iPTMnetiP47742.

Expressioni

Tissue specificityi

Retina.

Gene expression databases

ExpressionAtlasiP47742. baseline and differential.
GenevisibleiP47742. RN.

Interactioni

Subunit structurei

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Interacts with SQSTM1.1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009973.

Structurei

3D structure databases

ProteinModelPortaliP47742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP47742.
KOiK05190.
OMAiRIVANNH.
OrthoDBiEOG712TVZ.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008059. GABAAa_rho2_rcpt.
IPR008057. GABAAa_rho_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01670. GABAARRHO.
PR01672. GABAARRHO2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P47742-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPYFMRLALF LFCLMALVES RKPRRKRWTG HLETSKPSHL YKKNLDVTKI
60 70 80 90 100
RTGKPRPLLR VEDHDFTMRP AFGGPAIPVG VDVQVESLDS ISEVDMDFTM
110 120 130 140 150
TLYLRHYWRD ERLAFPSSSN RSMTFDGRLV KKIWVPDVFF VHSKRSFTHD
160 170 180 190 200
TTTDNIMLRV FPDGHVLYSM RITVTAMCNM DFSHFPLDSQ TCSLELESYA
210 220 230 240 250
YTDEDLMLYW KNGDESLKTD EKISLSQFLI QKFHTTSRLA FYSSTGWYNR
260 270 280 290 300
LYINFTLRRH IFFFLLQTYF PATLMVMLSW VSFWIDHRAV PARVSLGIMT
310 320 330 340 350
VLTMSTIITG VNASMPRVSY IRAVDIYLWV SFVFVFLSVL EYAAVNYLTT
360 370 380 390 400
VQEQKERKLR DKFPCTCGML HSRTMTLDGS YSESEANSLA GYPRSHILPE
410 420 430 440 450
EERQDKIVVH LALNSELTSS RKKGLLKGQM GLYIFQNTHA IDKYSRLIFP
460
AFYIVFNLIY WSVFS
Length:465
Mass (Da):54,296
Last modified:October 31, 2012 - v3
Checksum:i3A182C429C750028
GO
Isoform 2 (identifier: P47742-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVKSQGIFPCSCCSPVPACCVIDVCRM

Note: Isoform 2 could be translated from an upstream initiator ATG located in frame within the first coding exon. The probability of a signal peptide within this isoform is very low. No experimental confirmation available.
Show »
Length:491
Mass (Da):57,024
Checksum:i0F02DA97312F46EC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MVKSQGIFPCSCCSPVPACC VIDVCRM in isoform 2. CuratedVSP_044375

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38494 mRNA. Translation: BAA07506.1.
PIRiI56523.
RefSeqiNP_058988.1. NM_017292.2. [P47742-1]
UniGeneiRn.48659.

Genome annotation databases

EnsembliENSRNOT00000009973; ENSRNOP00000009973; ENSRNOG00000007490. [P47742-1]
GeneIDi29695.
KEGGirno:29695.
UCSCiRGD:61902. rat. [P47742-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38494 mRNA. Translation: BAA07506.1.
PIRiI56523.
RefSeqiNP_058988.1. NM_017292.2. [P47742-1]
UniGeneiRn.48659.

3D structure databases

ProteinModelPortaliP47742.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009973.

PTM databases

iPTMnetiP47742.

Proteomic databases

PaxDbiP47742.
PRIDEiP47742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009973; ENSRNOP00000009973; ENSRNOG00000007490. [P47742-1]
GeneIDi29695.
KEGGirno:29695.
UCSCiRGD:61902. rat. [P47742-1]

Organism-specific databases

CTDi2570.
RGDi61902. Gabrr2.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP47742.
KOiK05190.
OMAiRIVANNH.
OrthoDBiEOG712TVZ.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-RNO-975298. Ligand-gated ion channel transport.
R-RNO-977442. GABA A (rho) receptor activation.

Miscellaneous databases

PROiP47742.

Gene expression databases

ExpressionAtlasiP47742. baseline and differential.
GenevisibleiP47742. RN.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008059. GABAAa_rho2_rcpt.
IPR008057. GABAAa_rho_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01670. GABAARRHO.
PR01672. GABAARRHO2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of GABAA receptor subunits in rat retina: cloning of the rat GABAA receptor rho 2-subunit cDNA."
    Ogurusu T., Taira H., Shingai R.
    J. Neurochem. 65:964-968(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Retina.
  2. "ZIP3, a new splice variant of the PKC-zeta-interacting protein family, binds to GABAC receptors, PKC-zeta, and Kv beta 2."
    Croci C., Brandstaetter J.H., Enz R.
    J. Biol. Chem. 278:6128-6135(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SQSTM1.

Entry informationi

Entry nameiGBRR2_RAT
AccessioniPrimary (citable) accession number: P47742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 31, 2012
Last modified: June 8, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.