P47741 (TNR4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor receptor superfamily member 4

Alternative name(s):
OX40 antigen
OX40L receptor
CD_antigen=CD134

Gene names

Name:	Tnfrsf4
Synonyms:	Ox40, Txgp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for TNFSF4/OX40L/GP34.
Subunit structure	Interacts with TRAF2, TRAF3 and TRAF5 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 4 TNFR-Cys repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular defense response Inferred from mutant phenotype PubMed 10586044. Source: MGI inflammatory response Inferred from mutant phenotype PubMed 14730361. Source: MGI negative regulation of T cell apoptotic process Inferred from mutant phenotype PubMed 11567634. Source: BHF-UCL negative regulation of cytokine secretion Inferred from physical interaction PubMed 14635034. Source: MGI negative regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 7749983. Source: BHF-UCL negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 7749983. Source: BHF-UCL positive regulation of B cell proliferation Inferred from mutant phenotype PubMed 7749983. Source: BHF-UCL positive regulation of immunoglobulin secretion Inferred from direct assay PubMed 7749983. Source: BHF-UCL regulation of protein kinase activity Inferred from mutant phenotype PubMed 14730361. Source: MGI
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 16301745PubMed 19008373. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	tumor necrosis factor-activated receptor activity Inferred from physical interaction PubMed 14635034. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Traf2	P39429	3	EBI-520001,EBI-520016
Traf3	Q60803	3	EBI-520001,EBI-520135

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 272	253	Tumor necrosis factor receptor superfamily member 4		PRO_0000034555
Regions
	Topological domain	20 – 211	192	Extracellular Potential
	Transmembrane	212 – 236	25	Helical; Potential
	Topological domain	237 – 272	36	Cytoplasmic Potential
	Repeat	26 – 61	36	TNFR-Cys 1
	Repeat	62 – 103	42	TNFR-Cys 2
	Repeat	104 – 124	21	TNFR-Cys 3; truncated
	Repeat	125 – 165	41	TNFR-Cys 4
Amino acid modifications
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 38		By similarity
	Disulfide bond	39 ↔ 52		By similarity
	Disulfide bond	42 ↔ 60		By similarity
	Disulfide bond	63 ↔ 77		By similarity
	Disulfide bond	80 ↔ 95		By similarity
	Disulfide bond	83 ↔ 103		By similarity
	Disulfide bond	105 ↔ 123		By similarity
	Disulfide bond	126 ↔ 139		By similarity
	Disulfide bond	145 ↔ 164		By similarity
Experimental info
	Sequence conflict	15	1	A → G in CAA59476. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47741 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 06E7BB4156F0D08E	FASTA	272	30,154
	10 20 30 40 50 60 MYVWVQQPTA LLLLALTLGV TARRLNCVKH TYPSGHKCCR ECQPGHGMVS RCDHTRDTLC 70 80 90 100 110 120 HPCETGFYNE AVNYDTCKQC TQCNHRSGSE LKQNCTPTQD TVCRCRPGTQ PRQDSGYKLG 130 140 150 160 170 180 VDCVPCPPGH FSPGNNQACK PWTNCTLSGK QTRHPASDSL DAVCEDRSLL ATLLWETQRP 190 200 210 220 230 240 TFRPTTVQST TVWPRTSELP SPPTLVTPEG PAFAVLLGLG LGLLAPLTVL LALYLLRKAW 250 260 270 RLPNTPKPCW GNSFRTPIQE EHTDAHFTLA KI

References

[1]	"Cloning of mouse Ox40: a T cell activation marker that may mediate T-B cell interactions." Calderhead D.M., Buhlmann J.E., van den Eertwegh A.J., Claassen E., Noelle R.J., Fell H. J. Immunol. 151:5261-5271(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Gene structure and chromosomal localization of the mouse homologue of rat OX40 protein." Birkeland M.L., Copeland N.G., Gilbert D.J., Jenkins N.A., Barclay A.N. Eur. J. Immunol. 25:926-930(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21674 mRNA. Translation: CAA79772.1. X85214 Genomic DNA. Translation: CAA59476.1.
IPI	IPI00111237.
PIR	I48700.
RefSeq	NP_035789.1. NM_011659.2.
UniGene	Mm.13885.
3D structure databases
ProteinModelPortal	P47741.
SMR	P47741. Positions 25-166.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6239N.
IntAct	P47741. 4 interactions.
Proteomic databases
PRIDE	P47741.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	22163.
KEGG	mmu:22163.
Organism-specific databases
CTD	7293.
MGI	MGI:104512. Tnfrsf4.
Phylogenomic databases
eggNOG	NOG43906.
HOGENOM	HOG000049154.
HOVERGEN	HBG005031.
InParanoid	P47741.
KO	K05142.
OrthoDB	EOG4JQ3ZC.
Gene expression databases
Genevestigator	P47741.
GermOnline	ENSMUSG00000029075. Mus musculus.
Family and domain databases
InterPro	IPR001368. TNFR/NGFR_Cys_rich_reg. IPR020445. TNFR_4. [Graphical view]
Pfam	PF00020. TNFR_c6. 2 hits. [Graphical view]
PRINTS	PR01921. TNFACTORR4.
SMART	SM00208. TNFR. 3 hits. [Graphical view]
PROSITE	PS00652. TNFR_NGFR_1. 2 hits. PS50050. TNFR_NGFR_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	302094.
SOURCE	Search...

Entry information

Entry name

TNR4_MOUSE

Accession

Primary (citable) accession number: P47741

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program