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Protein

Aldehyde dehydrogenase, dimeric NADP-preferring

Gene

Aldh3a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes and in preventing corneal damage caused by ultraviolet light.2 Publications

Catalytic activityi

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei210 – 2101By similarity
Active sitei244 – 2441By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi188 – 1936NAD or NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.2.1.5. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase, dimeric NADP-preferring (EC:1.2.1.5)
Alternative name(s):
Aldehyde dehydrogenase 4
Aldehyde dehydrogenase family 3 member A1
Dioxin-inducible aldehyde dehydrogenase 3
Gene namesi
Name:Aldh3a1
Synonyms:Ahd-4, Ahd4, Aldh3, Aldh4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1353451. Aldh3a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 453452Aldehyde dehydrogenase, dimeric NADP-preferringPRO_0000056471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei178 – 1781N6-acetyllysineBy similarity
Modified residuei194 – 1941N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP47739.
PaxDbiP47739.
PRIDEiP47739.

PTM databases

PhosphoSiteiP47739.

Expressioni

Tissue specificityi

Constitutively expressed in cornea, stomach, skin, bladder and lungs. Lowest expression levels in lungs and bladder.1 Publication

Gene expression databases

CleanExiMM_ALDH3A1.
ExpressionAtlasiP47739. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP47739. 1 interaction.
MINTiMINT-4087533.
STRINGi10090.ENSMUSP00000019246.

Structurei

3D structure databases

ProteinModelPortaliP47739.
SMRiP47739. Positions 3-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00390000002825.
HOVERGENiHBG050483.
InParanoidiP47739.
KOiK00129.
OMAiQDEAYIH.
TreeFamiTF314264.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNISSIVNR ARDAFNSGKT RPLQFRVEQL EALQRMINEN LKGISKALAS
60 70 80 90 100
NLRKNEWTSY YEEVAHVLDE IDFTIKGLSD WAEDEPVAKT RQTQEDDLYI
110 120 130 140 150
HSEPLGVVLV IGAWNYPFNL TIQPMVGAIA AGNAVVLKPS EVSDHMADLL
160 170 180 190 200
STLIPQYMDK DLYPVIKGGV PETTELLKEK FDHIMYTGST AVGKIVMAAA
210 220 230 240 250
AKHLTPVTLE LGGKSPCYVD KDCDLDVACR RIAWGKFMNS GQTCVAPDYI
260 270 280 290 300
LCDPSIQNEI VEKLKKSLKD FYGEDAKQSH DYGRIINDRH FQRVINLIDS
310 320 330 340 350
KKVAHGGTWD QPSRYIAPTI LVDVDPQSPV MQEEIFGPVM PIVCVRSLDE
360 370 380 390 400
AIKFINQREK PLALYVFSNN DKVIKKMIAE TSSGGVTAND VIVHITVPTL
410 420 430 440 450
PFGGVGNSGM GAYHGKKSFE TFSHRRSCLV RSLRNEEANK ARYPPSPAKM

PRH
Length:453
Mass (Da):50,481
Last modified:October 3, 2012 - v2
Checksum:i7B4EA1CC56B5FAA1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881A → G in AAA20670 (PubMed:8148869).Curated
Sequence conflicti88 – 881A → R in AAD15964 (PubMed:10376761).Curated

Polymorphismi

There are two alleles, Ald3a1a and Ald3a1c. Ald3a1c codes for a low activity enzyme and is associated with extensive corneal clouding after exposure to ultraviolet light. Ald3a1a encodes the high activity enzyme.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti154 – 1541I → N in allele Ald3a1c. 1 Publication
Natural varianti305 – 3051H → R in allele Ald3a1c. 1 Publication
Natural varianti352 – 3521I → V in allele Ald3a1c. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12785 mRNA. Translation: AAA20670.1.
AF072815 mRNA. Translation: AAD15964.1.
AL646093 Genomic DNA. Translation: CAI25900.1.
CCDSiCCDS24808.1.
RefSeqiNP_001106196.1. NM_001112725.1.
NP_031462.2. NM_007436.2.
XP_006532089.1. XM_006532026.1.
XP_006532090.1. XM_006532027.2.
UniGeneiMm.4257.

Genome annotation databases

EnsembliENSMUST00000019246; ENSMUSP00000019246; ENSMUSG00000019102.
ENSMUST00000108716; ENSMUSP00000104356; ENSMUSG00000019102.
GeneIDi11670.
KEGGimmu:11670.
UCSCiuc007jhd.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12785 mRNA. Translation: AAA20670.1.
AF072815 mRNA. Translation: AAD15964.1.
AL646093 Genomic DNA. Translation: CAI25900.1.
CCDSiCCDS24808.1.
RefSeqiNP_001106196.1. NM_001112725.1.
NP_031462.2. NM_007436.2.
XP_006532089.1. XM_006532026.1.
XP_006532090.1. XM_006532027.2.
UniGeneiMm.4257.

3D structure databases

ProteinModelPortaliP47739.
SMRiP47739. Positions 3-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP47739. 1 interaction.
MINTiMINT-4087533.
STRINGi10090.ENSMUSP00000019246.

PTM databases

PhosphoSiteiP47739.

Proteomic databases

MaxQBiP47739.
PaxDbiP47739.
PRIDEiP47739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019246; ENSMUSP00000019246; ENSMUSG00000019102.
ENSMUST00000108716; ENSMUSP00000104356; ENSMUSG00000019102.
GeneIDi11670.
KEGGimmu:11670.
UCSCiuc007jhd.2. mouse.

Organism-specific databases

CTDi218.
MGIiMGI:1353451. Aldh3a1.

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00390000002825.
HOVERGENiHBG050483.
InParanoidiP47739.
KOiK00129.
OMAiQDEAYIH.
TreeFamiTF314264.

Enzyme and pathway databases

BRENDAi1.2.1.5. 3474.

Miscellaneous databases

NextBioi279295.
PROiP47739.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ALDH3A1.
ExpressionAtlasiP47739. baseline and differential.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036492. ALDH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse dioxin-inducible cytosolic aldehyde dehydrogenase-3: AHD4 cDNA sequence, genetic mapping, and differences in mRNA levels."
    Vasiliou V., Reuter S.F., Kozak C.A., Nebert D.W.
    Pharmacogenetics 3:281-290(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H.
  2. "Organization and characterization of the murine cytosolic TCDD-inducible aldehyde dehydrogenase gene (ahd4)."
    Vasiliou V., Reuter S.F., Nebert D.W.
    Toxicologist 14:410-410(1994)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Four amino acid changes are associated with the Aldh3a1 locus polymorphism in mice which may be responsible for corneal sensitivity to ultraviolet light."
    Shiao T., Tran P., Siegel D., Lee J., Vasiliou V.
    Pharmacogenetics 9:145-153(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANTS ALD3A1C ASN-154; ARG-305 AND VAL-352.
    Strain: DBA/2 and SWR/J.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "Structurally normal corneas in aldehyde dehydrogenase 3a1-deficient mice."
    Nees D.W., Wawrousek E.F., Robison W.G. Jr., Piatigorsky J.
    Mol. Cell. Biol. 22:849-855(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: CD-1.

Entry informationi

Entry nameiAL3A1_MOUSE
AccessioniPrimary (citable) accession number: P47739
Secondary accession number(s): B1ATI7, Q9R203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 3, 2012
Last modified: July 22, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.