Reviewed, UniProtKB/Swiss-Prot P47739 (AL3A1_MOUSE)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase, dimeric NADP-preferring
    EC=1.2.1.5
Alternative name(s):
    Aldehyde dehydrogenase family 3 member A1
    Aldehyde dehydrogenase 4
    Dioxin-inducible aldehyde dehydrogenase 3

Gene names

Name:	Aldh3a1
Synonyms:	Ahd-4, Ahd4, Aldh3, Aldh4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes and in preventing corneal damage caused by ultraviolet light.
Catalytic activity	An aldehyde + NAD(P)(+) + H(2)O = an acid + NAD(P)H.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Constitutively expressed in cornea, stomach, skin, bladder and lungs. Lowest expression levels in lungs and bladder.
Polymorphism	There are two alleles, Ald3a1a and Ald3a1c. Ald3a1c codes for a low activity enzyme and is associated with extensive corneal clouding after exposure to ultraviolet light. Ald3a1a encodes the high activity enzyme.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	3-chloroallyl aldehyde dehydrogenase activity Ref.4 Inferred from direct assay. Source: MGI alcohol dehydrogenase (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB aldehyde dehydrogenase (NAD) activity Inferred from sequence or structural similarity. Source: UniProtKB aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Aldehyde dehydrogenase, dimeric NADP-preferring		PRO_0000056471
Regions
	Nucleotide binding	188 – 193	6	NAD By similarity
Sites
	Active site	210	1	By similarity
	Active site	244	1	By similarity
Natural variations
	Natural variant	88	1	G → R in allele Ald3a1c.
	Natural variant	154	1	I → N in allele Ald3a1c.
	Natural variant	305	1	H → R in allele Ald3a1c.
	Natural variant	352	1	I → V in allele Ald3a1c.

Sequences

	Sequence		Length	Mass (Da)
	P47739-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9D94FE30094AD76A	FASTA	453	50,467
	10 20 30 40 50 60 MSNISSIVNR ARDAFNSGKT RPLQFRVEQL EALQRMINEN LKGISKALAS NLRKNEWTSY 70 80 90 100 110 120 YEEVAHVLDE IDFTIKGLSD WAEDEPVGKT RQTQEDDLYI HSEPLGVVLV IGAWNYPFNL 130 140 150 160 170 180 TIQPMVGAIA AGNAVVLKPS EVSDHMADLL STLIPQYMDK DLYPVIKGGV PETTELLKEK 190 200 210 220 230 240 FDHIMYTGST AVGKIVMAAA AKHLTPVTLE LGGKSPCYVD KDCDLDVACR RIAWGKFMNS 250 260 270 280 290 300 GQTCVAPDYI LCDPSIQNEI VEKLKKSLKD FYGEDAKQSH DYGRIINDRH FQRVINLIDS 310 320 330 340 350 360 KKVAHGGTWD QPSRYIAPTI LVDVDPQSPV MQEEIFGPVM PIVCVRSLDE AIKFINQREK 370 380 390 400 410 420 PLALYVFSNN DKVIKKMIAE TSSGGVTAND VIVHITVPTL PFGGVGNSGM GAYHGKKSFE 430 440 450 TFSHRRSCLV RSLRNEEANK ARYPPSPAKM PRH

References

[1]	"Mouse dioxin-inducible cytosolic aldehyde dehydrogenase-3: AHD4 cDNA sequence, genetic mapping, and differences in mRNA levels." Vasiliou V., Reuter S.F., Kozak C.A., Nebert D.W. Pharmacogenetics 3:281-290(1993) [PubMed: 8148869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H.
[2]	"Organization and characterization of the murine cytosolic TCDD-inducible aldehyde dehydrogenase gene (ahd4)." Vasiliou V., Reuter S.F., Nebert D.W. Toxicologist 14:410-410(1994) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Four amino acid changes are associated with the Aldh3a1 locus polymorphism in mice which may be responsible for corneal sensitivity to ultraviolet light." Shiao T., Tran P., Siegel D., Lee J., Vasiliou V. Pharmacogenetics 9:145-153(1999) [PubMed: 10376761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANTS ALD3A1C ARG-88; ASN-154; ARG-305 AND VAL-352. Strain: DBA/2 and SWR/J.
[4]	"Structurally normal corneas in aldehyde dehydrogenase 3a1-deficient mice." Nees D.W., Wawrousek E.F., Robison W.G. Jr., Piatigorsky J. Mol. Cell. Biol. 22:849-855(2002) [PubMed: 11784860] [Abstract] Cited for: FUNCTION. Strain: CD-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12785 mRNA. Translation: AAA20670.1. AF072815 mRNA. Translation: AAD15964.1.
UniGene	Mm.4257
3D structure databases
HSSP	HSSP built from PDB template 1AD3 based on UniProtKB P11883.
SMR	P47739. Positions 3-448.
ModBase	Search...
PTM databases
PhosphoSite	P47739.
Genome annotation databases
Ensembl	ENSMUSG00000019102. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1353451. Aldh3a1.
Phylogenomic databases
HOGENOM	P47739.
HOVERGEN	P47739.
Gene expression databases
ArrayExpress	P47739.
CleanEx	MM_ALDH3A1.
GermOnline	ENSMUSG00000019102. Mus musculus.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR012394. Ald_DHase_NAD(P). IPR015590. Aldehyde_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF15. ALDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF036492. ALDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

AL3A1_MOUSE

Accession

Primary (citable) accession number: P47739
Secondary accession number(s): Q9R203

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)