Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P47739 (AL3A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aldehyde dehydrogenase, dimeric NADP-preferring

EC=1.2.1.5
Alternative name(s):
Aldehyde dehydrogenase 4
Aldehyde dehydrogenase family 3 member A1
Dioxin-inducible aldehyde dehydrogenase 3
Gene names
Name:Aldh3a1
Synonyms:Ahd-4, Ahd4, Aldh3, Aldh4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes and in preventing corneal damage caused by ultraviolet light. Ref.3 Ref.5

Catalytic activity

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

Constitutively expressed in cornea, stomach, skin, bladder and lungs. Lowest expression levels in lungs and bladder. Ref.3

Polymorphism

There are two alleles, Ald3a1a and Ald3a1c. Ald3a1c codes for a low activity enzyme and is associated with extensive corneal clouding after exposure to ultraviolet light. Ald3a1a encodes the high activity enzyme.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandNAD
NADP
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

cellular aldehyde metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

oxidation-reduction process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to nutrient

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Non-traceable author statement Ref.1. Source: UniProtKB

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

   Molecular_function3-chloroallyl aldehyde dehydrogenase activity

Inferred from direct assay PubMed 12610736PubMed 742739. Source: MGI

alcohol dehydrogenase (NADP+) activity

Inferred from sequence or structural similarity. Source: UniProtKB

aldehyde dehydrogenase (NAD) activity

Inferred from sequence or structural similarity. Source: UniProtKB

aldehyde dehydrogenase [NAD(P)+] activity

Inferred from electronic annotation. Source: UniProtKB-EC

oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Inferred from direct assay Ref.3. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 453452Aldehyde dehydrogenase, dimeric NADP-preferring
PRO_0000056471

Regions

Nucleotide binding188 – 1936NAD or NADP By similarity

Sites

Active site2101 By similarity
Active site2441 By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1781N6-acetyllysine By similarity
Modified residue1941N6-acetyllysine By similarity

Natural variations

Natural variant1541I → N in allele Ald3a1c. Ref.3
Natural variant3051H → R in allele Ald3a1c. Ref.3
Natural variant3521I → V in allele Ald3a1c. Ref.3

Experimental info

Sequence conflict881A → G in AAA20670. Ref.1
Sequence conflict881A → R in AAD15964. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P47739 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 7B4EA1CC56B5FAA1

FASTA45350,481
        10         20         30         40         50         60 
MSNISSIVNR ARDAFNSGKT RPLQFRVEQL EALQRMINEN LKGISKALAS NLRKNEWTSY 

        70         80         90        100        110        120 
YEEVAHVLDE IDFTIKGLSD WAEDEPVAKT RQTQEDDLYI HSEPLGVVLV IGAWNYPFNL 

       130        140        150        160        170        180 
TIQPMVGAIA AGNAVVLKPS EVSDHMADLL STLIPQYMDK DLYPVIKGGV PETTELLKEK 

       190        200        210        220        230        240 
FDHIMYTGST AVGKIVMAAA AKHLTPVTLE LGGKSPCYVD KDCDLDVACR RIAWGKFMNS 

       250        260        270        280        290        300 
GQTCVAPDYI LCDPSIQNEI VEKLKKSLKD FYGEDAKQSH DYGRIINDRH FQRVINLIDS 

       310        320        330        340        350        360 
KKVAHGGTWD QPSRYIAPTI LVDVDPQSPV MQEEIFGPVM PIVCVRSLDE AIKFINQREK 

       370        380        390        400        410        420 
PLALYVFSNN DKVIKKMIAE TSSGGVTAND VIVHITVPTL PFGGVGNSGM GAYHGKKSFE 

       430        440        450 
TFSHRRSCLV RSLRNEEANK ARYPPSPAKM PRH 

« Hide

References

« Hide 'large scale' references
[1]"Mouse dioxin-inducible cytosolic aldehyde dehydrogenase-3: AHD4 cDNA sequence, genetic mapping, and differences in mRNA levels."
Vasiliou V., Reuter S.F., Kozak C.A., Nebert D.W.
Pharmacogenetics 3:281-290(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H.
[2]"Organization and characterization of the murine cytosolic TCDD-inducible aldehyde dehydrogenase gene (ahd4)."
Vasiliou V., Reuter S.F., Nebert D.W.
Toxicologist 14:410-410(1994)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Four amino acid changes are associated with the Aldh3a1 locus polymorphism in mice which may be responsible for corneal sensitivity to ultraviolet light."
Shiao T., Tran P., Siegel D., Lee J., Vasiliou V.
Pharmacogenetics 9:145-153(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANTS ALD3A1C ASN-154; ARG-305 AND VAL-352.
Strain: DBA/2 and SWR/J.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"Structurally normal corneas in aldehyde dehydrogenase 3a1-deficient mice."
Nees D.W., Wawrousek E.F., Robison W.G. Jr., Piatigorsky J.
Mol. Cell. Biol. 22:849-855(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: CD-1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12785 mRNA. Translation: AAA20670.1.
AF072815 mRNA. Translation: AAD15964.1.
AL646093 Genomic DNA. Translation: CAI25900.1.
RefSeqNP_001106196.1. NM_001112725.1.
NP_031462.2. NM_007436.2.
XP_006532089.1. XM_006532026.1.
XP_006532090.1. XM_006532027.1.
UniGeneMm.4257.

3D structure databases

ProteinModelPortalP47739.
SMRP47739. Positions 3-448.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP47739. 1 interaction.
MINTMINT-4087533.

PTM databases

PhosphoSiteP47739.

Proteomic databases

PaxDbP47739.
PRIDEP47739.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019246; ENSMUSP00000019246; ENSMUSG00000019102.
ENSMUST00000108716; ENSMUSP00000104356; ENSMUSG00000019102.
GeneID11670.
KEGGmmu:11670.
UCSCuc007jhd.2. mouse.

Organism-specific databases

CTD218.
MGIMGI:1353451. Aldh3a1.

Phylogenomic databases

eggNOGCOG1012.
GeneTreeENSGT00390000002825.
HOVERGENHBG050483.
InParanoidP47739.
KOK00129.
OMAHHFQRVM.
TreeFamTF314264.

Gene expression databases

ArrayExpressP47739.
CleanExMM_ALDH3A1.
GenevestigatorP47739.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012394. Aldehyde_DH_NAD(P).
[Graphical view]
PANTHERPTHR11699:SF15. PTHR11699:SF15. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFPIRSF036492. ALDH. 1 hit.
SUPFAMSSF53720. SSF53720. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279295.
PROP47739.
SOURCESearch...

Entry information

Entry nameAL3A1_MOUSE
AccessionPrimary (citable) accession number: P47739
Secondary accession number(s): B1ATI7, Q9R203
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot