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P47739

- AL3A1_MOUSE

UniProt

P47739 - AL3A1_MOUSE

Protein

Aldehyde dehydrogenase, dimeric NADP-preferring

Gene

Aldh3a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes and in preventing corneal damage caused by ultraviolet light.2 Publications

    Catalytic activityi

    An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei210 – 2101By similarity
    Active sitei244 – 2441By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi188 – 1936NAD or NADPBy similarity

    GO - Molecular functioni

    1. 3-chloroallyl aldehyde dehydrogenase activity Source: MGI
    2. alcohol dehydrogenase (NADP+) activity Source: UniProtKB
    3. aldehyde dehydrogenase (NAD) activity Source: UniProtKB
    4. aldehyde dehydrogenase [NAD(P)+] activity Source: UniProtKB-EC
    5. oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Source: UniProtKB

    GO - Biological processi

    1. aging Source: Ensembl
    2. cellular aldehyde metabolic process Source: UniProtKB
    3. oxidation-reduction process Source: UniProtKB
    4. positive regulation of cell proliferation Source: Ensembl
    5. response to cAMP Source: Ensembl
    6. response to drug Source: Ensembl
    7. response to glucocorticoid Source: Ensembl
    8. response to hypoxia Source: Ensembl
    9. response to nutrient Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldehyde dehydrogenase, dimeric NADP-preferring (EC:1.2.1.5)
    Alternative name(s):
    Aldehyde dehydrogenase 4
    Aldehyde dehydrogenase family 3 member A1
    Dioxin-inducible aldehyde dehydrogenase 3
    Gene namesi
    Name:Aldh3a1
    Synonyms:Ahd-4, Ahd4, Aldh3, Aldh4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1353451. Aldh3a1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: UniProtKB
    3. endoplasmic reticulum Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 453452Aldehyde dehydrogenase, dimeric NADP-preferringPRO_0000056471Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei178 – 1781N6-acetyllysineBy similarity
    Modified residuei194 – 1941N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP47739.
    PaxDbiP47739.
    PRIDEiP47739.

    PTM databases

    PhosphoSiteiP47739.

    Expressioni

    Tissue specificityi

    Constitutively expressed in cornea, stomach, skin, bladder and lungs. Lowest expression levels in lungs and bladder.1 Publication

    Gene expression databases

    ArrayExpressiP47739.
    CleanExiMM_ALDH3A1.
    GenevestigatoriP47739.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiP47739. 1 interaction.
    MINTiMINT-4087533.

    Structurei

    3D structure databases

    ProteinModelPortaliP47739.
    SMRiP47739. Positions 3-448.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1012.
    GeneTreeiENSGT00390000002825.
    HOVERGENiHBG050483.
    InParanoidiP47739.
    KOiK00129.
    OMAiDHIEDAL.
    TreeFamiTF314264.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR012394. Aldehyde_DH_NAD(P).
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036492. ALDH. 1 hit.
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P47739-1 [UniParc]FASTAAdd to Basket

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    MSNISSIVNR ARDAFNSGKT RPLQFRVEQL EALQRMINEN LKGISKALAS    50
    NLRKNEWTSY YEEVAHVLDE IDFTIKGLSD WAEDEPVAKT RQTQEDDLYI 100
    HSEPLGVVLV IGAWNYPFNL TIQPMVGAIA AGNAVVLKPS EVSDHMADLL 150
    STLIPQYMDK DLYPVIKGGV PETTELLKEK FDHIMYTGST AVGKIVMAAA 200
    AKHLTPVTLE LGGKSPCYVD KDCDLDVACR RIAWGKFMNS GQTCVAPDYI 250
    LCDPSIQNEI VEKLKKSLKD FYGEDAKQSH DYGRIINDRH FQRVINLIDS 300
    KKVAHGGTWD QPSRYIAPTI LVDVDPQSPV MQEEIFGPVM PIVCVRSLDE 350
    AIKFINQREK PLALYVFSNN DKVIKKMIAE TSSGGVTAND VIVHITVPTL 400
    PFGGVGNSGM GAYHGKKSFE TFSHRRSCLV RSLRNEEANK ARYPPSPAKM 450
    PRH 453
    Length:453
    Mass (Da):50,481
    Last modified:October 3, 2012 - v2
    Checksum:i7B4EA1CC56B5FAA1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti88 – 881A → G in AAA20670. (PubMed:8148869)Curated
    Sequence conflicti88 – 881A → R in AAD15964. (PubMed:10376761)Curated

    Polymorphismi

    There are two alleles, Ald3a1a and Ald3a1c. Ald3a1c codes for a low activity enzyme and is associated with extensive corneal clouding after exposure to ultraviolet light. Ald3a1a encodes the high activity enzyme.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti154 – 1541I → N in allele Ald3a1c. 1 Publication
    Natural varianti305 – 3051H → R in allele Ald3a1c. 1 Publication
    Natural varianti352 – 3521I → V in allele Ald3a1c. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12785 mRNA. Translation: AAA20670.1.
    AF072815 mRNA. Translation: AAD15964.1.
    AL646093 Genomic DNA. Translation: CAI25900.1.
    CCDSiCCDS24808.1.
    RefSeqiNP_001106196.1. NM_001112725.1.
    NP_031462.2. NM_007436.2.
    XP_006532089.1. XM_006532026.1.
    XP_006532090.1. XM_006532027.1.
    UniGeneiMm.4257.

    Genome annotation databases

    EnsembliENSMUST00000019246; ENSMUSP00000019246; ENSMUSG00000019102.
    ENSMUST00000108716; ENSMUSP00000104356; ENSMUSG00000019102.
    GeneIDi11670.
    KEGGimmu:11670.
    UCSCiuc007jhd.2. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12785 mRNA. Translation: AAA20670.1 .
    AF072815 mRNA. Translation: AAD15964.1 .
    AL646093 Genomic DNA. Translation: CAI25900.1 .
    CCDSi CCDS24808.1.
    RefSeqi NP_001106196.1. NM_001112725.1.
    NP_031462.2. NM_007436.2.
    XP_006532089.1. XM_006532026.1.
    XP_006532090.1. XM_006532027.1.
    UniGenei Mm.4257.

    3D structure databases

    ProteinModelPortali P47739.
    SMRi P47739. Positions 3-448.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P47739. 1 interaction.
    MINTi MINT-4087533.

    PTM databases

    PhosphoSitei P47739.

    Proteomic databases

    MaxQBi P47739.
    PaxDbi P47739.
    PRIDEi P47739.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019246 ; ENSMUSP00000019246 ; ENSMUSG00000019102 .
    ENSMUST00000108716 ; ENSMUSP00000104356 ; ENSMUSG00000019102 .
    GeneIDi 11670.
    KEGGi mmu:11670.
    UCSCi uc007jhd.2. mouse.

    Organism-specific databases

    CTDi 218.
    MGIi MGI:1353451. Aldh3a1.

    Phylogenomic databases

    eggNOGi COG1012.
    GeneTreei ENSGT00390000002825.
    HOVERGENi HBG050483.
    InParanoidi P47739.
    KOi K00129.
    OMAi DHIEDAL.
    TreeFami TF314264.

    Miscellaneous databases

    NextBioi 279295.
    PROi P47739.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P47739.
    CleanExi MM_ALDH3A1.
    Genevestigatori P47739.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR012394. Aldehyde_DH_NAD(P).
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036492. ALDH. 1 hit.
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse dioxin-inducible cytosolic aldehyde dehydrogenase-3: AHD4 cDNA sequence, genetic mapping, and differences in mRNA levels."
      Vasiliou V., Reuter S.F., Kozak C.A., Nebert D.W.
      Pharmacogenetics 3:281-290(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H.
    2. "Organization and characterization of the murine cytosolic TCDD-inducible aldehyde dehydrogenase gene (ahd4)."
      Vasiliou V., Reuter S.F., Nebert D.W.
      Toxicologist 14:410-410(1994)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Four amino acid changes are associated with the Aldh3a1 locus polymorphism in mice which may be responsible for corneal sensitivity to ultraviolet light."
      Shiao T., Tran P., Siegel D., Lee J., Vasiliou V.
      Pharmacogenetics 9:145-153(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANTS ALD3A1C ASN-154; ARG-305 AND VAL-352.
      Strain: DBA/2 and SWR/J.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "Structurally normal corneas in aldehyde dehydrogenase 3a1-deficient mice."
      Nees D.W., Wawrousek E.F., Robison W.G. Jr., Piatigorsky J.
      Mol. Cell. Biol. 22:849-855(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: CD-1.

    Entry informationi

    Entry nameiAL3A1_MOUSE
    AccessioniPrimary (citable) accession number: P47739
    Secondary accession number(s): B1ATI7, Q9R203
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3