Reviewed, UniProtKB/Swiss-Prot P47738 (ALDH2_MOUSE)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase, mitochondrial
    EC=1.2.1.3
Alternative name(s):
    ALDH class 2
    AHD-M1
    ALDHI
    ALDH-E2

Gene names

Name:	Aldh2
Synonyms:	Ahd-1, Ahd1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is capable of converting retinaldehyde to retinoic acid.
Catalytic activity	An aldehyde + NAD+ + H2O = an acid + NADH.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion matrix.
Induction	By retinoic acid; 3-5 fold increase.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion By similarity
	Chain	20 – 519	500	Aldehyde dehydrogenase, mitochondrial		PRO_0000007169
Regions
	Nucleotide binding	264 – 269	6	NAD By similarity
Sites
	Active site	287	1	Proton acceptor By similarity
	Active site	321	1	Nucleophile By similarity
	Site	188	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	20	1	N-acetylserine Probable
	Modified residue	370	1	N6-acetyllysine Ref.6
Experimental info
	Sequence conflict	88 – 89	2	AF → C in AAC60691. Ref.2
	Sequence conflict	181	1	Missing in AAC60691. Ref.2
	Sequence conflict	227	1	I → S in AAC60691. Ref.2
	Sequence conflict	344	1	R → G in AAC60691. Ref.2
	Sequence conflict	370	1	K → N in AAC60691. Ref.2
	Sequence conflict	378	1	S → M in AAC60691. Ref.2
	Sequence conflict	476	1	D → V in AAC60691. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47738-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 200806F63D48F4DA	FASTA	519	56,538
	10 20 30 40 50 60 MLRAALTTVR RGPRLSRLLS AAATSAVPAP NHQPEVFCNQ IFINNEWHDA VSRKTFPTVN 70 80 90 100 110 120 PSTGEVICQV AEGNKEDVDK AVKAARAAFQ LGSPWRRMDA SDRGRLLYRL ADLIERDRTY 130 140 150 160 170 180 LAALETLDNG KPYVISYLVD LDMVLKCLRY YAGWADKYHG KTIPIDGDFF SYTRHEPVGV 190 200 210 220 230 240 CGQIIPWNFP LLMQAWKLGP ALATGNVVVM KVAEQTPLTA LYVANLIKEA GFPPGVVNIV 250 260 270 280 290 300 PGFGPTAGAA IASHEGVDKV AFTGSTEVGH LIQVAAGSSN LKRVTLELGG KSPNIIMSDA 310 320 330 340 350 360 DMDWAVEQAH FALFFNQGQC CCAGSRTFVQ ENVYDEFVER SVARAKSRVV GNPFDSRTEQ 370 380 390 400 410 420 GPQVDETQFK KILGYIKSGQ QEGAKLLCGG GAAADRGYFI QPTVFGDVKD GMTIAKEEIF 430 440 450 460 470 480 GPVMQILKFK TIEEVVGRAN DSKYGLAAAV FTKDLDKANY LSQALQAGTV WINCYDVFGA 490 500 510 QSPFGGYKMS GSGRELGEYG LQAYTEVKTV TVKVPQKNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the gene encoding mouse mitochondrial aldehyde dehydrogenase." Chang C., Yoshida A. Gene 148:331-336(1994) [PubMed: 7958964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"Enzymatic conversion of retinaldehyde to retinoic acid by cloned murine cytosolic and mitochondrial aldehyde dehydrogenases." Chen M., Achkar C., Gudas L.J. Mol. Pharmacol. 46:88-96(1994) [PubMed: 8058062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[4]	Lubec G., Kang S.U., Klug S., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 54-80; 87-96; 162-174; 198-228; 260-282; 327-340; 349-370; 386-409; 417-428; 431-438 AND 444-453, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[5]	"Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor." Caubin J., Iglesias T., Bernal J., Munoz A., Marquez G., Barbero J.L., Zaballos A. Nucleic Acids Res. 22:4132-4138(1994) [PubMed: 7937138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 472-509.
[6]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-370, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U07235 mRNA. Translation: AAA64636.1. S71509 mRNA. Translation: AAC60691.1. BC005476 mRNA. Translation: AAH05476.1. Z32545 Genomic DNA. No translation available.
IPI	IPI00111218.
PIR	I48966.
RefSeq	NP_033786.1.
UniGene	Mm.284446
3D structure databases
HSSP	HSSP built from PDB template 1O01 based on UniProtKB P05091.
SMR	P47738. Positions 26-519.
ModBase	Search...
PTM databases
PhosphoSite	P47738.
2-D gel databases
SWISS-2DPAGE	P47738.
REPRODUCTION-2DPAGE	P47738.
Proteomic databases
PRIDE	P47738.
Genome annotation databases
Ensembl	ENSMUSG00000029455. Mus musculus. [Contig view]
GeneID	11669.
KEGG	mmu:11669.
Organism-specific databases
MGI	MGI:99600. Aldh2.
Phylogenomic databases
HOGENOM	P47738.
HOVERGEN	P47738.
OMA	P47738. PFDSQTE.
Enzyme and pathway databases
BRENDA	1.2.1.3. 244.
Gene expression databases
ArrayExpress	P47738.
Bgee	P47738.
CleanEx	MM_ALDH2.
GermOnline	ENSMUSG00000029455. Mus musculus.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279291.
SOURCE	Search...

Entry information

Entry name

ALDH2_MOUSE

Accession

Primary (citable) accession number: P47738

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)