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Protein

Aldehyde dehydrogenase, mitochondrial

Gene

Aldh2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is capable of converting retinaldehyde to retinoic acid.

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei188 – 1881Transition state stabilizerBy similarity
Active sitei287 – 2871Proton acceptorPROSITE-ProRule annotation
Active sitei321 – 3211NucleophilePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi264 – 2696NADBy similarity

GO - Molecular functioni

  1. aldehyde dehydrogenase (NAD) activity Source: MGI

GO - Biological processi

  1. ethanol catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_231054. Metabolism of serotonin.
REACT_260489. Ethanol oxidation.
UniPathwayiUPA00780; UER00768.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase, mitochondrial (EC:1.2.1.3)
Alternative name(s):
AHD-M1
ALDH class 2
ALDH-E2
ALDHI
Gene namesi
Name:Aldh2
Synonyms:Ahd-1, Ahd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:99600. Aldh2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. mitochondrial matrix Source: UniProtKB-SubCell
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1919MitochondrionBy similarityAdd
BLAST
Chaini20 – 519500Aldehyde dehydrogenase, mitochondrialPRO_0000007169Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201N-acetylserineCurated
Modified residuei54 – 541N6-acetyllysine1 Publication
Modified residuei75 – 751N6-acetyllysine1 Publication
Modified residuei80 – 801N6-acetyllysine1 Publication
Modified residuei161 – 1611N6-acetyllysine1 Publication
Modified residuei370 – 3701N6-acetyllysine1 Publication
Modified residuei377 – 3771N6-acetyllysine1 Publication
Modified residuei385 – 3851N6-acetyllysine1 Publication
Modified residuei409 – 4091N6-acetyllysine1 Publication
Modified residuei428 – 4281N6-acetyllysine1 Publication
Modified residuei430 – 4301N6-acetyllysine2 Publications
Modified residuei443 – 4431N6-acetyllysine1 Publication
Modified residuei453 – 4531N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP47738.
PaxDbiP47738.
PRIDEiP47738.

2D gel databases

REPRODUCTION-2DPAGEP47738.
SWISS-2DPAGEP47738.
UCD-2DPAGEP47738.

PTM databases

PhosphoSiteiP47738.

Expressioni

Inductioni

By retinoic acid; 3-5 fold increase.

Gene expression databases

BgeeiP47738.
CleanExiMM_ALDH2.
ExpressionAtlasiP47738. baseline and differential.
GenevestigatoriP47738.

Interactioni

Subunit structurei

Homotetramer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Sirt3Q8R1042EBI-2308120,EBI-6999888

Protein-protein interaction databases

IntActiP47738. 5 interactions.
MINTiMINT-1859437.

Structurei

3D structure databases

ProteinModelPortaliP47738.
SMRiP47738. Positions 26-519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP47738.
KOiK00128.
OMAiHLIMEAA.
OrthoDBiEOG7PS1F7.
PhylomeDBiP47738.
TreeFamiTF300455.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47738-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRAALTTVR RGPRLSRLLS AAATSAVPAP NHQPEVFCNQ IFINNEWHDA
60 70 80 90 100
VSRKTFPTVN PSTGEVICQV AEGNKEDVDK AVKAARAAFQ LGSPWRRMDA
110 120 130 140 150
SDRGRLLYRL ADLIERDRTY LAALETLDNG KPYVISYLVD LDMVLKCLRY
160 170 180 190 200
YAGWADKYHG KTIPIDGDFF SYTRHEPVGV CGQIIPWNFP LLMQAWKLGP
210 220 230 240 250
ALATGNVVVM KVAEQTPLTA LYVANLIKEA GFPPGVVNIV PGFGPTAGAA
260 270 280 290 300
IASHEGVDKV AFTGSTEVGH LIQVAAGSSN LKRVTLELGG KSPNIIMSDA
310 320 330 340 350
DMDWAVEQAH FALFFNQGQC CCAGSRTFVQ ENVYDEFVER SVARAKSRVV
360 370 380 390 400
GNPFDSRTEQ GPQVDETQFK KILGYIKSGQ QEGAKLLCGG GAAADRGYFI
410 420 430 440 450
QPTVFGDVKD GMTIAKEEIF GPVMQILKFK TIEEVVGRAN DSKYGLAAAV
460 470 480 490 500
FTKDLDKANY LSQALQAGTV WINCYDVFGA QSPFGGYKMS GSGRELGEYG
510
LQAYTEVKTV TVKVPQKNS
Length:519
Mass (Da):56,538
Last modified:February 1, 1996 - v1
Checksum:i200806F63D48F4DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 892AF → C in AAC60691. (PubMed:8058062)Curated
Sequence conflicti181 – 1811Missing in AAC60691. (PubMed:8058062)Curated
Sequence conflicti227 – 2271I → S in AAC60691. (PubMed:8058062)Curated
Sequence conflicti344 – 3441R → G in AAC60691. (PubMed:8058062)Curated
Sequence conflicti370 – 3701K → N in AAC60691. (PubMed:8058062)Curated
Sequence conflicti378 – 3781S → M in AAC60691. (PubMed:8058062)Curated
Sequence conflicti476 – 4761D → V in AAC60691. (PubMed:8058062)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07235 mRNA. Translation: AAA64636.1.
S71509 mRNA. Translation: AAC60691.1.
BC005476 mRNA. Translation: AAH05476.1.
Z32545 Genomic DNA. No translation available.
CCDSiCCDS19638.1.
PIRiI48966.
RefSeqiNP_033786.1. NM_009656.3.
UniGeneiMm.284446.

Genome annotation databases

EnsembliENSMUST00000031411; ENSMUSP00000031411; ENSMUSG00000029455.
GeneIDi11669.
KEGGimmu:11669.
UCSCiuc008zjt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07235 mRNA. Translation: AAA64636.1.
S71509 mRNA. Translation: AAC60691.1.
BC005476 mRNA. Translation: AAH05476.1.
Z32545 Genomic DNA. No translation available.
CCDSiCCDS19638.1.
PIRiI48966.
RefSeqiNP_033786.1. NM_009656.3.
UniGeneiMm.284446.

3D structure databases

ProteinModelPortaliP47738.
SMRiP47738. Positions 26-519.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP47738. 5 interactions.
MINTiMINT-1859437.

PTM databases

PhosphoSiteiP47738.

2D gel databases

REPRODUCTION-2DPAGEP47738.
SWISS-2DPAGEP47738.
UCD-2DPAGEP47738.

Proteomic databases

MaxQBiP47738.
PaxDbiP47738.
PRIDEiP47738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031411; ENSMUSP00000031411; ENSMUSG00000029455.
GeneIDi11669.
KEGGimmu:11669.
UCSCiuc008zjt.1. mouse.

Organism-specific databases

CTDi217.
MGIiMGI:99600. Aldh2.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP47738.
KOiK00128.
OMAiHLIMEAA.
OrthoDBiEOG7PS1F7.
PhylomeDBiP47738.
TreeFamiTF300455.

Enzyme and pathway databases

UniPathwayiUPA00780; UER00768.
ReactomeiREACT_231054. Metabolism of serotonin.
REACT_260489. Ethanol oxidation.

Miscellaneous databases

ChiTaRSiAldh2. mouse.
NextBioi279291.
PROiP47738.
SOURCEiSearch...

Gene expression databases

BgeeiP47738.
CleanExiMM_ALDH2.
ExpressionAtlasiP47738. baseline and differential.
GenevestigatoriP47738.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the gene encoding mouse mitochondrial aldehyde dehydrogenase."
    Chang C., Yoshida A.
    Gene 148:331-336(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Enzymatic conversion of retinaldehyde to retinoic acid by cloned murine cytosolic and mitochondrial aldehyde dehydrogenases."
    Chen M., Achkar C., Gudas L.J.
    Mol. Pharmacol. 46:88-96(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. Lubec G., Kang S.U., Klug S., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 54-80; 87-96; 162-174; 198-228; 260-282; 327-340; 349-370; 386-409; 417-428; 431-438 AND 444-453, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  5. "Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor."
    Caubin J., Iglesias T., Bernal J., Munoz A., Marquez G., Barbero J.L., Zaballos A.
    Nucleic Acids Res. 22:4132-4138(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 472-509.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-54; LYS-75; LYS-80; LYS-161; LYS-370; LYS-377; LYS-385; LYS-409; LYS-428; LYS-430; LYS-443 AND LYS-453, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiALDH2_MOUSE
AccessioniPrimary (citable) accession number: P47738
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 4, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.