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Protein

Rap1 GTPase-activating protein 1

Gene

RAP1GAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • Ras GTPase binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000076864-MONOMER.
ReactomeiR-HSA-392517. Rap1 signalling.
R-HSA-8853659. RET signaling.
SignaLinkiP47736.
SIGNORiP47736.

Names & Taxonomyi

Protein namesi
Recommended name:
Rap1 GTPase-activating protein 1
Short name:
Rap1GAP
Short name:
Rap1GAP1
Gene namesi
Name:RAP1GAP
Synonyms:KIAA0474, RAP1GA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9858. RAP1GAP.

Subcellular locationi

GO - Cellular componenti

  • axon Source: Ensembl
  • cytosol Source: UniProtKB
  • dendrite Source: Ensembl
  • early endosome Source: Ensembl
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • neuronal cell body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100F → E: Impaired dimerization; when associated with E-173. 1 Publication1
Mutagenesisi173L → E: Impaired dimerization; when associated with E-100. 1 Publication1
Mutagenesisi207E → A: Reduces GTPase activation. 1 Publication1
Mutagenesisi267H → A: Abolishes GTPase activation. 1 Publication1
Mutagenesisi286R → A: Reduces GTPase activation. 1 Publication1
Mutagenesisi287H → A: Abolishes GTPase activation. 1 Publication1
Mutagenesisi290N → A or K: Abolishes GTPase activation. 1 Publication1
Mutagenesisi291D → A: Abolishes GTPase activation. 1 Publication1
Mutagenesisi388R → A or P: Reduces GTPase activation. 1 Publication1

Organism-specific databases

DisGeNETi5909.
OpenTargetsiENSG00000076864.
PharmGKBiPA34220.

Polymorphism and mutation databases

BioMutaiRAP1GAP.
DMDMi215273877.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000567431 – 663Rap1 GTPase-activating protein 1Add BLAST663

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei441PhosphoserineCombined sources1
Modified residuei484PhosphoserineCombined sources1
Modified residuei499PhosphoserineCombined sources1
Modified residuei515PhosphoserineCombined sources1
Modified residuei541PhosphoserineBy similarity1
Modified residuei542PhosphoserineBy similarity1
Isoform 3 (identifier: P47736-3)
Modified residuei17PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47736.
PaxDbiP47736.
PeptideAtlasiP47736.
PRIDEiP47736.

PTM databases

iPTMnetiP47736.
PhosphoSitePlusiP47736.

Expressioni

Tissue specificityi

Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.1 Publication

Inductioni

By 12-O-tetradecanoylphorbol-13-acetate (TPA) in promyelocytic HL-60 cells.1 Publication

Gene expression databases

BgeeiENSG00000076864.
CleanExiHS_RAP1GAP.
ExpressionAtlasiP47736. baseline and differential.
GenevisibleiP47736. HS.

Organism-specific databases

HPAiCAB003851.
HPA001922.

Interactioni

Subunit structurei

Homodimer and heterodimer with RAP1B.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAP1BP612243EBI-722307,EBI-358143

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • Ras GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111844. 18 interactors.
IntActiP47736. 10 interactors.
MINTiMINT-3308298.
STRINGi9606.ENSP00000290101.

Structurei

Secondary structure

1663
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi85 – 88Combined sources4
Helixi89 – 93Combined sources5
Turni94 – 96Combined sources3
Beta strandi100 – 106Combined sources7
Turni107 – 109Combined sources3
Beta strandi110 – 121Combined sources12
Beta strandi124 – 132Combined sources9
Beta strandi137 – 143Combined sources7
Helixi153 – 160Combined sources8
Helixi177 – 186Combined sources10
Beta strandi192 – 200Combined sources9
Helixi207 – 211Combined sources5
Helixi218 – 227Combined sources10
Beta strandi228 – 233Combined sources6
Beta strandi245 – 247Combined sources3
Beta strandi252 – 258Combined sources7
Beta strandi263 – 268Combined sources6
Helixi269 – 271Combined sources3
Helixi282 – 288Combined sources7
Beta strandi292 – 300Combined sources9
Helixi306 – 308Combined sources3
Beta strandi316 – 323Combined sources8
Beta strandi327 – 329Combined sources3
Beta strandi331 – 338Combined sources8
Beta strandi354 – 358Combined sources5
Helixi359 – 376Combined sources18
Helixi380 – 409Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SRQX-ray2.90A/B/C/D75-415[»]
3BRWX-ray3.40A/B/C75-415[»]
ProteinModelPortaliP47736.
SMRiP47736.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47736.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 17GoLocoPROSITE-ProRule annotationAdd BLAST17
Domaini181 – 397Rap-GAPPROSITE-ProRule annotationAdd BLAST217

Sequence similaritiesi

Contains 1 GoLoco domain.PROSITE-ProRule annotation
Contains 1 Rap-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3686. Eukaryota.
ENOG410XTIX. LUCA.
GeneTreeiENSGT00760000119182.
HOGENOMiHOG000231640.
HOVERGENiHBG016371.
InParanoidiP47736.
KOiK17700.
PhylomeDBiP47736.
TreeFamiTF318626.

Family and domain databases

InterProiIPR003109. GoLoco_motif.
IPR000331. Rap_GAP_dom.
[Graphical view]
PfamiPF02188. GoLoco. 1 hit.
PF02145. Rap_GAP. 1 hit.
[Graphical view]
SMARTiSM00390. GoLoco. 1 hit.
[Graphical view]
SUPFAMiSSF111347. SSF111347. 1 hit.
PROSITEiPS50877. GOLOCO. 1 hit.
PS50085. RAPGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P47736-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIEKMQGSRM DEQRCSFPPP LKTEEDYIPY PSVHEVLGRE GPFPLILLPQ
60 70 80 90 100
FGGYWIEGTN HEITSIPETE PLQSPTTKVK LECNPTARIY RKHFLGKEHF
110 120 130 140 150
NYYSLDAALG HLVFSLKYDV IGDQEHLRLL LRTKCRTYHD VIPISCLTEF
160 170 180 190 200
PNVVQMAKLV CEDVNVDRFY PVLYPKASRL IVTFDEHVIS NNFKFGVIYQ
210 220 230 240 250
KLGQTSEEEL FSTNEESPAF VEFLEFLGQK VKLQDFKGFR GGLDVTHGQT
260 270 280 290 300
GTESVYCNFR NKEIMFHVST KLPYTEGDAQ QLQRKRHIGN DIVAVVFQDE
310 320 330 340 350
NTPFVPDMIA SNFLHAYVVV QAEGGGPDGP LYKVSVTARD DVPFFGPPLP
360 370 380 390 400
DPAVFRKGPE FQEFLLTKLI NAEYACYKAE KFAKLEERTR AALLETLYEE
410 420 430 440 450
LHIHSQSMMG LGGDEDKMEN GSGGGGFFES FKRVIRSRSQ SMDAMGLSNK
460 470 480 490 500
KPNTVSTSHS GSFAPNNPDL AKAAGISLIV PGKSPTRKKS GPFGSRRSSA
510 520 530 540 550
IGIENIQEVQ EKRESPPAGQ KTPDSGHVSQ EPKSENSSTQ SSPEMPTTKN
560 570 580 590 600
RAETAAQRAE ALKDFSRSSS SASSFASVVE ETEGVDGEDT GLESVSSSGT
610 620 630 640 650
PHKRDSFIYS TWLEDSVSTT SGGSSPGPSR SPHPDAGKLG DPACPEIKIQ
660
LEASEQHMPQ LGC
Length:663
Mass (Da):73,361
Last modified:November 25, 2008 - v2
Checksum:i89B307CC67F975DD
GO
Isoform 2 (identifier: P47736-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     476-476: I → ISLLIPGKSASRFGRRGSAIGIGTVEE
     626-633: Missing.

Show »
Length:681
Mass (Da):75,187
Checksum:i7B2B680087CFCCB4
GO
Isoform 3 (identifier: P47736-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAQLRPAVPPGRPRRGSLPAGASWQNTDLFEM
     280-280: Missing.

Show »
Length:693
Mass (Da):76,592
Checksum:iBEB784BF1F8E67F3
GO
Isoform 4 (identifier: P47736-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSGRKRSFTFGAYGGVDKSFTSRRSVWRSDGQNQHFPQALDLSRVNLVPSYTPSLYPKNTDLFEM

Note: No experimental confirmation available.
Show »
Length:727
Mass (Da):80,645
Checksum:iB106FC67B06E1615
GO

Sequence cautioni

The sequence AAH54490 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA32319 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti304F → L in AAH54490 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047792107A → T.1 PublicationCorresponds to variant rs2275363dbSNPEnsembl.1
Natural variantiVAR_035547257C → R in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_035548609Y → C in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs147394161dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0402601M → MAQLRPAVPPGRPRRGSLPA GASWQNTDLFEM in isoform 3. 1 Publication1
Alternative sequenceiVSP_0470251M → MSGRKRSFTFGAYGGVDKSF TSRRSVWRSDGQNQHFPQAL DLSRVNLVPSYTPSLYPKNT DLFEM in isoform 4. Curated1
Alternative sequenceiVSP_040261280Missing in isoform 3. 1 Publication1
Alternative sequenceiVSP_035256476I → ISLLIPGKSASRFGRRGSAI GIGTVEE in isoform 2. 1 Publication1
Alternative sequenceiVSP_035257626 – 633Missing in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64788 mRNA. Translation: AAA60252.1.
AB007943 mRNA. Translation: BAA32319.3. Different initiation.
AL359815 Genomic DNA. Translation: CAI16250.1.
AL359815 Genomic DNA. Translation: CAI16255.1.
CH471134 Genomic DNA. Translation: EAW94980.1.
CH471134 Genomic DNA. Translation: EAW94981.1.
AB003930 mRNA. Translation: BAA83674.1.
BC054490 mRNA. Translation: AAH54490.1. Different initiation.
CCDSiCCDS218.1. [P47736-1]
CCDS53276.1. [P47736-2]
CCDS53277.1. [P47736-4]
PIRiA39897.
B39897.
RefSeqiNP_001139129.1. NM_001145657.1. [P47736-2]
NP_001139130.1. NM_001145658.1. [P47736-4]
NP_002876.2. NM_002885.2. [P47736-1]
XP_005246012.2. XM_005245955.3. [P47736-2]
XP_016857459.1. XM_017001970.1. [P47736-2]
XP_016857462.1. XM_017001973.1. [P47736-1]
XP_016857473.1. XM_017001984.1. [P47736-2]
XP_016857475.1. XM_017001986.1. [P47736-1]
XP_016857476.1. XM_017001987.1. [P47736-1]
XP_016857477.1. XM_017001988.1. [P47736-1]
UniGeneiHs.148178.

Genome annotation databases

EnsembliENST00000374765; ENSP00000363897; ENSG00000076864. [P47736-1]
ENST00000495204; ENSP00000434033; ENSG00000076864. [P47736-4]
ENST00000542643; ENSP00000441661; ENSG00000076864. [P47736-2]
GeneIDi5909.
KEGGihsa:5909.
UCSCiuc001bew.4. human. [P47736-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64788 mRNA. Translation: AAA60252.1.
AB007943 mRNA. Translation: BAA32319.3. Different initiation.
AL359815 Genomic DNA. Translation: CAI16250.1.
AL359815 Genomic DNA. Translation: CAI16255.1.
CH471134 Genomic DNA. Translation: EAW94980.1.
CH471134 Genomic DNA. Translation: EAW94981.1.
AB003930 mRNA. Translation: BAA83674.1.
BC054490 mRNA. Translation: AAH54490.1. Different initiation.
CCDSiCCDS218.1. [P47736-1]
CCDS53276.1. [P47736-2]
CCDS53277.1. [P47736-4]
PIRiA39897.
B39897.
RefSeqiNP_001139129.1. NM_001145657.1. [P47736-2]
NP_001139130.1. NM_001145658.1. [P47736-4]
NP_002876.2. NM_002885.2. [P47736-1]
XP_005246012.2. XM_005245955.3. [P47736-2]
XP_016857459.1. XM_017001970.1. [P47736-2]
XP_016857462.1. XM_017001973.1. [P47736-1]
XP_016857473.1. XM_017001984.1. [P47736-2]
XP_016857475.1. XM_017001986.1. [P47736-1]
XP_016857476.1. XM_017001987.1. [P47736-1]
XP_016857477.1. XM_017001988.1. [P47736-1]
UniGeneiHs.148178.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SRQX-ray2.90A/B/C/D75-415[»]
3BRWX-ray3.40A/B/C75-415[»]
ProteinModelPortaliP47736.
SMRiP47736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111844. 18 interactors.
IntActiP47736. 10 interactors.
MINTiMINT-3308298.
STRINGi9606.ENSP00000290101.

PTM databases

iPTMnetiP47736.
PhosphoSitePlusiP47736.

Polymorphism and mutation databases

BioMutaiRAP1GAP.
DMDMi215273877.

Proteomic databases

MaxQBiP47736.
PaxDbiP47736.
PeptideAtlasiP47736.
PRIDEiP47736.

Protocols and materials databases

DNASUi5909.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374765; ENSP00000363897; ENSG00000076864. [P47736-1]
ENST00000495204; ENSP00000434033; ENSG00000076864. [P47736-4]
ENST00000542643; ENSP00000441661; ENSG00000076864. [P47736-2]
GeneIDi5909.
KEGGihsa:5909.
UCSCiuc001bew.4. human. [P47736-1]

Organism-specific databases

CTDi5909.
DisGeNETi5909.
GeneCardsiRAP1GAP.
HGNCiHGNC:9858. RAP1GAP.
HPAiCAB003851.
HPA001922.
MIMi600278. gene.
neXtProtiNX_P47736.
OpenTargetsiENSG00000076864.
PharmGKBiPA34220.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3686. Eukaryota.
ENOG410XTIX. LUCA.
GeneTreeiENSGT00760000119182.
HOGENOMiHOG000231640.
HOVERGENiHBG016371.
InParanoidiP47736.
KOiK17700.
PhylomeDBiP47736.
TreeFamiTF318626.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000076864-MONOMER.
ReactomeiR-HSA-392517. Rap1 signalling.
R-HSA-8853659. RET signaling.
SignaLinkiP47736.
SIGNORiP47736.

Miscellaneous databases

ChiTaRSiRAP1GAP. human.
EvolutionaryTraceiP47736.
GeneWikiiRAP1GAP.
GenomeRNAii5909.
PROiP47736.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000076864.
CleanExiHS_RAP1GAP.
ExpressionAtlasiP47736. baseline and differential.
GenevisibleiP47736. HS.

Family and domain databases

InterProiIPR003109. GoLoco_motif.
IPR000331. Rap_GAP_dom.
[Graphical view]
PfamiPF02188. GoLoco. 1 hit.
PF02145. Rap_GAP. 1 hit.
[Graphical view]
SMARTiSM00390. GoLoco. 1 hit.
[Graphical view]
SUPFAMiSSF111347. SSF111347. 1 hit.
PROSITEiPS50877. GOLOCO. 1 hit.
PS50085. RAPGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPGP1_HUMAN
AccessioniPrimary (citable) accession number: P47736
Secondary accession number(s): J3QSS6
, O75062, Q5T3S9, Q5T3T4, Q7Z5S8, Q9UQ51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 25, 2008
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.