P47734 (FADH_METMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase
EC=1.1.1.284

Alternative name(s):
Glutathione-dependent formaldehyde dehydrogenase
Short name=FALDH
Short name=FDH

Gene names

Name:

fdh

Organism

Methylobacter marinus

Taxonomic identifier

34058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylobacter

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	The enzyme is equally active with NAD and NADP.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
Sequence caution	The sequence AAA65728.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	S-(hydroxymethyl)glutathione dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	S-(hydroxymethyl)glutathione dehydrogenase		PRO_0000160787
Sites
	Metal binding	38	1	Zinc 1; catalytic By similarity
	Metal binding	60	1	Zinc 1; catalytic By similarity
	Metal binding	90	1	Zinc 2 By similarity
	Metal binding	93	1	Zinc 2 By similarity
	Metal binding	96	1	Zinc 2 By similarity
	Metal binding	104	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47734 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 3926BE73EDF5713F	FASTA	390	42,221
	10 20 30 40 50 60 MKALCWHGKY DVRVDTVPDP IIVDPTDAIV KITATAICGS DLHLYDGYMP TMESGDILGH 70 80 90 100 110 120 EPMGEVVEVG SEVTSLKTGD RVVVPFTISC GHCFFCEKTL YSCCDTSNPN ADMARKAMGH 130 140 150 160 170 180 SPAGIFGYSH MLGGFAGGQA EYLRVPYADV GPIKITSDLP DDKVLFLSDI FPTGYMAAEN 190 200 210 220 230 240 AQIEPGDTVA VWGCGPVAQF VIQSCWMLGA GRVIAIDRVP ERLTMAQEHG KAEIIDFEEE 250 260 270 280 290 300 DVYDRLMAMT NGRGPDCCID AVGCEAHGAG SFDAVLDKAK AALYLGTDRP HVLREAIMCC 310 320 330 340 350 360 RKGGTVSIPG VYVGLLDQLP MGAAMNKGLT LKMGQTHVQR YLEPLLEKIE EDQIDPSFVI 370 380 390 THSLGLDEGP QAYKTFRDKE DGCIKVVLKP

References

[1]	"Sequence of the gene for a NAD(P)-dependent formaldehyde dehydrogenase (class III alcohol dehydrogenase) from a marine methanotroph Methylobacter marinus A45." Speer B.S., Chistoserdova L., Lidstrom M.E. FEMS Microbiol. Lett. 121:349-355(1994) [PubMed: 7926692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A45.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33464 Genomic DNA. Translation: AAA65728.1. Different initiation.
3D structure databases
ProteinModelPortal	P47734.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADH_METMR

Accession

Primary (citable) accession number: P47734

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 20, 2009
Last modified:	December 14, 2011
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program