P47719 (GYRA_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	MYCGA7230
ORF Names:	MGA_0612

Organism

Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]

Taxonomic identifier

710127 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	846 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 846	846	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145239
Sites
	Active site	132	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	55 – 57	3	HRR → TW in AAA57342. Ref.2
	Sequence conflict	91	1	S → G in AAA57342. Ref.2
	Sequence conflict	128	1	A → G in AAA57342. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P47719 [UniParc]. Last modified August 15, 2003. Version 2. Checksum: A5A16FEAAA5FB683	FASTA	846	94,333
	10 20 30 40 50 60 MNPNDKNNIK ELLNKTVVKE ASITKELETS FMEYAMSVIV SRALPESRDG LKPVHRRVLY 70 80 90 100 110 120 GAYTSGLTHD KPYRKSAQIV GHVMGKYHPH SDSAIYETMV RMAQPFSLRY MLIDGHGNFG 130 140 150 160 170 180 SIDGDSAAAM RYTEARLSKI SAEMLRNIDK DTVDFVDNYD ASEQEPIVLP SLFPNLLANG 190 200 210 220 230 240 SSGIAVGMAT NIPPHNLSEL IGGIKHLLVN ENATIEELKE FIKGPDFPTA AEILGETGIN 250 260 270 280 290 300 EYFNTGRGSV SVRAKSEIEE LANNKSNIVI TQIPYMVNKA NLINKIAELV KTEQIQGIAD 310 320 330 340 350 360 LRDESNREGI RIVIETKRDV IPEVLLNQLY KSTQLQTNFS VAMLALVNNQ PKVLNLKEAL 370 380 390 400 410 420 QIYIDHQFDI LLRKTNFELK KAKASAHIVE GLVIATNNID DVIETIKNAK DNEDAKNTLM 430 440 450 460 470 480 TKYELSDLQA KAILDMRLRS LSGLERENLQ KELAKLKELI KDLEEILQNK ERRIKIISDQ 490 500 510 520 530 540 LDEIDHKFGD ERRTKICYGL NSTIDNEQLI PVETVVITRS SKGYLKRIPI SAYKVQHRGG 550 560 570 580 590 600 VGVKGMNTYE DDDVESLIVC STHSDLLFFT NYGKVYRIRA HQVPLGSRIS KGIPAINLIS 610 620 630 640 650 660 IEKDEKLMSL LSINDYDSGY FFFSTKKGLV KRVKASEFSR IQNNGKIAIK LTENDSLFKV 670 680 690 700 710 720 IKTAGDEEIY IGVSSGLLVR FKEDVVRSMG RTAQGVIGVK FKNPNDEVIG LSSSHEGSLL 730 740 750 760 770 780 LAVCEKGVGK MTDREEYRMT NRGSKGVITI KVTPKTGNII NTQLVNGNEE LLMISSTGKI 790 800 810 820 830 840 VRVPLAEVSE QGRNTSGVKL ISLNEKETLQ SVAIFDVEQD DSQQVGSDNP DTELSSDDSN QDQDKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)." Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J. Microbiology 149:2307-2316(2003) [PubMed: 12949158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(low / passage 15 / clone 2).
[2]	"Sequence and transcriptional analysis of the genes encoding the class-II topoisomerase of Mycoplasma gallisepticum." Forsyth M.H., Sayed A.S., Geary S.J. Gene 163:161-162(1995) [PubMed: 7557470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-384. Strain: S6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015450 Genomic DNA. Translation: AAP57073.1. U18306 Genomic DNA. Translation: AAA57342.1.
RefSeq	NP_853505.1. NC_004829.2.
3D structure databases
ProteinModelPortal	P47719.
SMR	P47719. Positions 40-497.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1090305.
GenomeReviews	Gene locus MYCGA7230 in contig AE015450_GR.
KEGG	mga:MGA_0612.
PATRIC	20009369. VBIMycGal115357_0813.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	P47719.
ProtClustDB	PRK05560.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCGA

Accession

Primary (citable) accession number: P47719

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	August 15, 2003
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program