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Protein

Cytosolic phospholipase A2

Gene

Pla2g4a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1By similarity
Metal bindingi40 – 401Calcium 2By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi43 – 431Calcium 2By similarity
Metal bindingi65 – 651Calcium 1By similarity
Metal bindingi93 – 931Calcium 2By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Calcium 2By similarity
Active sitei228 – 2281NucleophileBy similarity
Active sitei548 – 5481Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • arachidonic acid secretion Source: UniProtKB
  • cellular response to antibiotic Source: MGI
  • icosanoid biosynthetic process Source: MGI
  • phospholipid catabolic process Source: InterPro
  • regulation of cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4. 3474.
ReactomeiREACT_271710. Acyl chain remodelling of PE.
REACT_281187. Arachidonic acid metabolism.
REACT_287069. Acyl chain remodelling of PC.
REACT_304133. Synthesis of PA.
REACT_306352. phospho-PLA2 pathway.
REACT_323162. Acyl chain remodeling of CL.
REACT_334244. ADP signalling through P2Y purinoceptor 1.
REACT_335533. Platelet sensitization by LDL.
REACT_340996. Hydrolysis of LPC.
REACT_346145. Acyl chain remodelling of PG.
REACT_346170. Acyl chain remodelling of PI.
REACT_351300. Acyl chain remodelling of PS.
SABIO-RKP47713.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Pla2g4a
Synonyms:Cpla2, Pla2g4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1195256. Pla2g4a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB
  • endoplasmic reticulum Source: MGI
  • Golgi apparatus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 748748Cytosolic phospholipase A2PRO_0000187263Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphothreonineBy similarity
Modified residuei437 – 4371Phosphoserine1 Publication
Modified residuei505 – 5051Phosphoserine; by MAPK1 Publication
Modified residuei726 – 7261Phosphoserine1 Publication
Modified residuei728 – 7281PhosphoserineBy similarity

Post-translational modificationi

Activated by phosphorylation at both Ser-505 and Ser-726.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47713.
PaxDbiP47713.
PRIDEiP47713.

PTM databases

PhosphoSiteiP47713.

Miscellaneous databases

PMAP-CutDBP47713.

Expressioni

Gene expression databases

BgeeiP47713.
ExpressionAtlasiP47713. baseline and differential.
GenevisibleiP47713. MM.

Interactioni

Protein-protein interaction databases

BioGridi202222. 6 interactions.
IntActiP47713. 14 interactions.
MINTiMINT-4106449.
STRINGi10090.ENSMUSP00000070868.

Structurei

3D structure databases

ProteinModelPortaliP47713.
SMRiP47713. Positions 13-720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2PROSITE-ProRule annotationAdd
BLAST
Domaini140 – 739600PLA2cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingCuratedAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257248.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP47713.
KOiK16342.
OMAiETLIHNR.
OrthoDBiEOG73V6JM.
PhylomeDBiP47713.
TreeFamiTF325228.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGTFGDMLD TPDPYVELFI
60 70 80 90 100
STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE
110 120 130 140 150
TLGTATFPVS SMKVGEKKEV PFIFNQVTEM ILEMSLEVCS CPDLRFSMAL
160 170 180 190 200
CDQEKTFRQQ RKENIKENMK KLLGPKKSEG LYSTRDVPVV AILGSGGGFR
210 220 230 240 250
AMVGFSGVMK ALYESGILDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPEE
260 270 280 290 300
INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIQNRMSM TLSSLKEKVN AARCPLPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSGS QNKGSTMEEE LENITAKHIV SNDSSDSDDE AQGPKGTENE
460 470 480 490 500
EAEKEYQSDN QASWVHRMLM ALVSDSALFN TREGRAGKVH NFMLGLNLNT
510 520 530 540 550
SYPLSPLRDF SSQDSFDDEL DAAVADPDEF ERIYEPLDVK SKKIHVVDSG
560 570 580 590 600
LTFNLPYPLI LRPQRGVDLI ISFDFSARPS DTSPPFKELL LAEKWAKMNK
610 620 630 640 650
LPFPKIDPYV FDREGLKECY VFKPKNPDVE KDCPTIIHFV LANINFRKYK
660 670 680 690 700
APGVLRETKE EKEIADFDIF DDPESPFSTF NFQYPNQAFK RLHDLMYFNT
710 720 730 740
LNNIDVIKDA IVESIEYRRQ NPSRCSVSLS NVEARKFFNK EFLSKPTV
Length:748
Mass (Da):85,222
Last modified:February 1, 1996 - v1
Checksum:i49D12BBB2911492A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72394 mRNA. Translation: AAB00796.1.
BC003816 mRNA. Translation: AAH03816.1.
CCDSiCCDS15352.1.
PIRiB39898.
RefSeqiNP_032895.1. NM_008869.4.
UniGeneiMm.4186.

Genome annotation databases

EnsembliENSMUST00000070200; ENSMUSP00000070868; ENSMUSG00000056220.
GeneIDi18783.
KEGGimmu:18783.
UCSCiuc007cxt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72394 mRNA. Translation: AAB00796.1.
BC003816 mRNA. Translation: AAH03816.1.
CCDSiCCDS15352.1.
PIRiB39898.
RefSeqiNP_032895.1. NM_008869.4.
UniGeneiMm.4186.

3D structure databases

ProteinModelPortaliP47713.
SMRiP47713. Positions 13-720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202222. 6 interactions.
IntActiP47713. 14 interactions.
MINTiMINT-4106449.
STRINGi10090.ENSMUSP00000070868.

Chemistry

BindingDBiP47713.
ChEMBLiCHEMBL2907.

PTM databases

PhosphoSiteiP47713.

Proteomic databases

MaxQBiP47713.
PaxDbiP47713.
PRIDEiP47713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070200; ENSMUSP00000070868; ENSMUSG00000056220.
GeneIDi18783.
KEGGimmu:18783.
UCSCiuc007cxt.1. mouse.

Organism-specific databases

CTDi5321.
MGIiMGI:1195256. Pla2g4a.

Phylogenomic databases

eggNOGiNOG257248.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP47713.
KOiK16342.
OMAiETLIHNR.
OrthoDBiEOG73V6JM.
PhylomeDBiP47713.
TreeFamiTF325228.

Enzyme and pathway databases

BRENDAi3.1.1.4. 3474.
ReactomeiREACT_271710. Acyl chain remodelling of PE.
REACT_281187. Arachidonic acid metabolism.
REACT_287069. Acyl chain remodelling of PC.
REACT_304133. Synthesis of PA.
REACT_306352. phospho-PLA2 pathway.
REACT_323162. Acyl chain remodeling of CL.
REACT_334244. ADP signalling through P2Y purinoceptor 1.
REACT_335533. Platelet sensitization by LDL.
REACT_340996. Hydrolysis of LPC.
REACT_346145. Acyl chain remodelling of PG.
REACT_346170. Acyl chain remodelling of PI.
REACT_351300. Acyl chain remodelling of PS.
SABIO-RKP47713.

Miscellaneous databases

NextBioi295044.
PMAP-CutDBP47713.
PROiP47713.
SOURCEiSearch...

Gene expression databases

BgeeiP47713.
ExpressionAtlasiP47713. baseline and differential.
GenevisibleiP47713. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP."
    Clark J.D., Lin L.-L., Kriz R.W., Ramesha C.S., Sultzman L.A., Lin A.Y., Milona N., Knopf J.L.
    Cell 65:1043-1051(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. Cited for: PHOSPHORYLATION AT SER-505 AND SER-726, ACTIVATION.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-437, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPA24A_MOUSE
AccessioniPrimary (citable) accession number: P47713
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.