Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic phospholipase A2

Gene

Pla2g4a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Calcium 1By similarity1
Metal bindingi40Calcium 2By similarity1
Metal bindingi41Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi43Calcium 1By similarity1
Metal bindingi43Calcium 2By similarity1
Metal bindingi65Calcium 1By similarity1
Metal bindingi93Calcium 2By similarity1
Metal bindingi94Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi95Calcium 2By similarity1
Active sitei228NucleophileBy similarity1
Active sitei548Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • arachidonic acid secretion Source: UniProtKB
  • cellular response to antibiotic Source: MGI
  • icosanoid biosynthetic process Source: MGI
  • phospholipid catabolic process Source: InterPro
  • regulation of cell proliferation Source: MGI

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4 3474
ReactomeiR-MMU-111995 phospho-PLA2 pathway
R-MMU-1482788 Acyl chain remodelling of PC
R-MMU-1482798 Acyl chain remodeling of CL
R-MMU-1482801 Acyl chain remodelling of PS
R-MMU-1482839 Acyl chain remodelling of PE
R-MMU-1482922 Acyl chain remodelling of PI
R-MMU-1482925 Acyl chain remodelling of PG
R-MMU-1483115 Hydrolysis of LPC
R-MMU-1483166 Synthesis of PA
R-MMU-2142753 Arachidonic acid metabolism
R-MMU-418592 ADP signalling through P2Y purinoceptor 1
R-MMU-432142 Platelet sensitization by LDL
R-MMU-6811436 COPI-independent Golgi-to-ER retrograde traffic
SABIO-RKiP47713

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Pla2g4a
Synonyms:Cpla2, Pla2g4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1195256 Pla2g4a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2907

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872631 – 748Cytosolic phospholipase A2Add BLAST748

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineBy similarity1
Modified residuei268PhosphothreonineBy similarity1
Modified residuei434PhosphoserineBy similarity1
Modified residuei435PhosphoserineCombined sources1
Modified residuei437PhosphoserineCombined sources1
Modified residuei505Phosphoserine; by MAPKCombined sources1 Publication1
Modified residuei515PhosphoserineBy similarity1
Cross-linki540Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki605Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei726PhosphoserineCombined sources1 Publication1
Modified residuei728PhosphoserineCombined sources1

Post-translational modificationi

Activated by phosphorylation at both Ser-505 and Ser-726.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP47713
PeptideAtlasiP47713
PRIDEiP47713

PTM databases

iPTMnetiP47713
PhosphoSitePlusiP47713
SwissPalmiP47713

Miscellaneous databases

PMAP-CutDBiP47713

Expressioni

Gene expression databases

BgeeiENSMUSG00000056220
ExpressionAtlasiP47713 baseline and differential
GenevisibleiP47713 MM

Interactioni

Protein-protein interaction databases

BioGridi202222, 11 interactors
IntActiP47713, 14 interactors
MINTiP47713
STRINGi10090.ENSMUSP00000070868

Chemistry databases

BindingDBiP47713

Structurei

3D structure databases

ProteinModelPortaliP47713
SMRiP47713
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 106C2PROSITE-ProRule annotationAdd BLAST102
Domaini140 – 739PLA2cPROSITE-ProRule annotationAdd BLAST600

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 178Phospholipid bindingCuratedAdd BLAST178

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG1012 Eukaryota
KOG1325 Eukaryota
ENOG410XR72 LUCA
GeneTreeiENSGT00550000074489
HOGENOMiHOG000115420
HOVERGENiHBG053479
InParanoidiP47713
KOiK16342
OMAiRFSMALC
OrthoDBiEOG091G0276
PhylomeDBiP47713
TreeFamiTF325228

Family and domain databases

Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR002642 LysoPLipase_cat_dom
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF01735 PLA2_B, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00022 PLAc, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS51210 PLA2C, 1 hit

Sequencei

Sequence statusi: Complete.

P47713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGTFGDMLD TPDPYVELFI
60 70 80 90 100
STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE
110 120 130 140 150
TLGTATFPVS SMKVGEKKEV PFIFNQVTEM ILEMSLEVCS CPDLRFSMAL
160 170 180 190 200
CDQEKTFRQQ RKENIKENMK KLLGPKKSEG LYSTRDVPVV AILGSGGGFR
210 220 230 240 250
AMVGFSGVMK ALYESGILDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPEE
260 270 280 290 300
INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIQNRMSM TLSSLKEKVN AARCPLPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSGS QNKGSTMEEE LENITAKHIV SNDSSDSDDE AQGPKGTENE
460 470 480 490 500
EAEKEYQSDN QASWVHRMLM ALVSDSALFN TREGRAGKVH NFMLGLNLNT
510 520 530 540 550
SYPLSPLRDF SSQDSFDDEL DAAVADPDEF ERIYEPLDVK SKKIHVVDSG
560 570 580 590 600
LTFNLPYPLI LRPQRGVDLI ISFDFSARPS DTSPPFKELL LAEKWAKMNK
610 620 630 640 650
LPFPKIDPYV FDREGLKECY VFKPKNPDVE KDCPTIIHFV LANINFRKYK
660 670 680 690 700
APGVLRETKE EKEIADFDIF DDPESPFSTF NFQYPNQAFK RLHDLMYFNT
710 720 730 740
LNNIDVIKDA IVESIEYRRQ NPSRCSVSLS NVEARKFFNK EFLSKPTV
Length:748
Mass (Da):85,222
Last modified:February 1, 1996 - v1
Checksum:i49D12BBB2911492A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72394 mRNA Translation: AAB00796.1
BC003816 mRNA Translation: AAH03816.1
CCDSiCCDS15352.1
PIRiB39898
RefSeqiNP_032895.1, NM_008869.4
UniGeneiMm.4186

Genome annotation databases

EnsembliENSMUST00000070200; ENSMUSP00000070868; ENSMUSG00000056220
GeneIDi18783
KEGGimmu:18783
UCSCiuc007cxt.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPA24A_MOUSE
AccessioniPrimary (citable) accession number: P47713
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health